• Korean Journal of Optics and Photonics
• /
• v.33 no.6
• /
• pp.260-266
• /
• 2022

A diffraction grating structure composed of two matching layers and two grating layers was formed, and a diffraction grating with high transmission diffraction efficiency in the -1st order was designed through an optimization technique. The designed diffraction grating had a transverse electric wave diffraction efficiency of 99.997% at the design center wavelength, and had a wavelength width of 80 nm and an incident angle width of 20.0° that maintained a diffraction efficiency of 95% or more. By performing the grating tolerance analysis, it was confirmed that the thickness tolerance for a diffraction efficiency of 95% or more was secured to at least 60 nm, and the diffraction efficiency could be maintained even in a trapezoidal shape with an internal angle of less than 10°.

• Proceedings of the Microbiological Society of Korea Conference
• /
• 1998.04a
• /
• pp.43.1-43.1
• /
• 1998

• International Journal of Fuzzy Logic and Intelligent Systems
• /
• v.2 no.1
• /
• pp.26-32
• /
• 2002

The objectives of this paper are twofold. First is to introduce a model for representing documents with semantics relatedness using rough sets but with tolerance relations instead of equivalence relations (TRSM). Second is to introduce two document hierarchical and nonhierarchical clustering algorithms based on this model and TRSM cluster-based information retrieval using these two algorithms. The experimental results show that TRSM offers an alterative approach to text clustering and information retrieval.

• Journal of the Korea Safety Management & Science
• /
• v.2 no.1
• /
• pp.89-97
• /
• 2000

This paper shows the study for development inprocess measuring magnetic sensor for tolerance control, First, we fabricated and annealed sample amorphous ribbons which were made of ($CO_{0.94}$$Fe_{0.06}$)$_{79}$$Si_{2}$$b_{19}$ having near zero magnetostrictive. It had 12［$\mu\textrm{m}$］ of thickness, 10［mm］ of length, and 2.5［mm］ of width. Then, we measured the magnetic characteristics such as frequency dependency over impedance variation rate and position change over impedance variation. As the results of the evaluation, we found that the sample ribbons had excellent sensor characteristics. Then, we made sensors by using the sample ribbons, As the results of the measurements, it proved that the ribbon could be used as excellent magnetic sensor for which it was possible to control inprocess measurement within the tolerance range(~$\mu\textrm{m}$ ).

• Design & Manufacturing
• /
• v.15 no.2
• /
• pp.42-48
• /
• 2021

In this paper, when manufacturing large/multi-mold parts (more than 30 core parts),A mold manufacturing (tolerance) management system was established through design-processing linkage.The mold manufacturing (tolerance) management system is a design-based measurement shape/measurement position determination system, M/C processing-linked measurement drive system,It is composed of four parts: CAD-linked measurement result analysis system and manager mold part quality management system.In addition, the constructed system was applied to the field and the effect of system construction was evaluated by comparing it with the existing process.As a result of the evaluation, the measurement precision is within 0.02mm, and the time it takes to measure after the end of processing is shorter than that of the existing process.(12 hours → 2 hours) It was shortened to 16.7%.In addition, it was confirmed that the time required for reprocessing after measurement was reduced by 25% (4 hours → 1 hour) compared to the existing process.

• Journal of Microbiology and Biotechnology
• /
• v.34 no.8
• /
• pp.1609-1616
• /
• 2024

The Burkholderia cepacia complex (Bcc) consists of opportunistic pathogens known to cause pneumonia in immunocompromised individuals, especially those with cystic fibrosis. Treating Bcc pneumonia is challenging due to the pathogens' high multidrug resistance. Therefore, inhalation therapy with tobramycin powder, which can achieve high antibiotic concentrations in the lungs, is a promising treatment option. In this study, we investigated potential mechanisms that could compromise the effectiveness of tobramycin therapy. By selecting for B. cenocepacia survivors against tobramycin, we identified three spontaneous mutations that disrupt a gene encoding a key enzyme in the biosynthesis of cobalamin (Vitamin B₁₂). This disruption may affect the production of succinyl-CoA by methylmalonyl-CoA mutase, which requires adenosylcobalamin as a cofactor. The depletion of cellular succinyl-CoA may impact the tricarboxylic acid (TCA) cycle, which becomes metabolically overloaded upon exposure to tobramycin. Consequently, the mutants exhibited significantly reduced reactive oxygen species (ROS) production. Both the wild-type and mutants showed tolerance to tobramycin and various other bactericidal antibiotics under microaerobic conditions. This suggests that compromised ROS-mediated killing, due to the impacted TCA cycle, underlies the mutants' tolerance to bactericidal antibiotics. The importance of ROS-mediated killing and the potential emergence of mutants that evade it through the depletion of cobalamin (Vitamin B₁₂) provide valuable insights for developing strategies to enhance antibiotic treatments of Bcc pneumonia.

• BMB Reports
• /
• v.43 no.5
• /
• pp.330-336
• /
• 2010

5-Aminolevulinate (ALA) is well-known as an essential biosynthetic precursor of all tetrapyrrole compounds, which has been suggested to improve plant salt tolerance by exogenous application. In this work, the gene encoding aminolevulinate synthase (ALA-S) in yeast (Saccharomyces cerevisiae Hem1) was introduced into the genome of Arabidopsis controlled by the Arabidopsis thaliana HemA1 gene promoter. All transgenic lines were able to transcribe the YHem1 gene, especially under light condition. The chimeric protein (YHem1-EGFP) was found co-localizing with the mitochondria in onion epidermal cells. The transgenic Arabidopsis plants could synthesize more endogenous ALA with higher levels of metabolites including chlorophyll and heme. When the $T_2$ homozygous seeds were cultured under NaCl stress, their germination and seedling growth were much better than the wild type. Therefore, introduction of ALA-S gene led to higher level of ALA metabolism with more salt tolerance in higher plants.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.115-115
• /
• 2017

Zinc finger proteins constitute a large family which has been studied to have various functions in different organisms. Tandem CCCH zinc finger proteins (TZFs), members of the zinc finger protein family, are known to participate as post-transcriptional regulators of gene expression in eukaryotes. Here, we showed that the OsZn15, a gene for tandem CCCH zinc finger protein, is induced by abiotic stress and its overexpression in transgenic rice plants (PGD1:OsZn15) gains higher drought tolerance. Gene expression analysis of promoter:GFP plants revealed that OsZn15 is specifically expressed in anther and embryo, but not in vegetative organs. In-field evaluation, grain yield was higher in the PGD1:OsZn15 than nontransgenic plants under drought conditions. Interestingly, OsZn15 is shown to not only localize at nucleus but also co-localize with both processing bodies (PB) and stress granules (SG), two messenger ribo-nucleoprotein complexes which are known to activate by forming cytoplasmic foci under stress conditions. In sum, these results suggest that OsZn15 increases drought stress tolerance of rice probably by participating in RNA turnover in PB and SG.

• IMMUNE NETWORK
• /
• v.8 no.2
• /
• pp.46-52
• /
• 2008

Background: Oral tolerance is defined by the inhibition of immune responsiveness to a protein previously exposed via the oral route. Protein antigens exposed via the oral route can be absorbed through the mucosal surfaces of the gastrointestinal tract and can make physical contact with immune cells residing in the intestinal lamina propria (LP). However, the mechanisms of oral tolerance and immune regulation in the intestines currently remain to be clearly elucidated. Methods: In order to determine the effect of oral protein antigen intake (ovalbumin, OVA) on the intestinal LP, we assessed the expression profile of the T cell receptor and the co-receptors on the cells from the intestines of the tolerant and immune mouse groups. Results: We determined that the proportion of OVA-specific B cells and ${\gamma}{\delta}$ T cells had decreased, but the CD8${\alpha}{\beta}$ and D8${\alpha}{\alpha}$ T cells were increased in the LP from the tolerant group. The proportion of CD8＋ T cells in the spleen did not evidence any significant differences between treatment groups. Conclusion: These results indicate that CD8＋ T cells in the intestinal LP may perform a regulatory role following antigen challenge via the oral route.

• Journal of Pharmaceutical Investigation
• /
• v.30 no.1
• /
• pp.47-50
• /
• 2000

A new technique has been developed for the preparation of Lactobacillus microcapsules to enhance the stability against high temperature, humidity, gastric acid and bile acid. Employing fluidized bed coating, primary sub-coating was processed in non-organic solvent system, so that Lactobacillus did not directly contact with organic solvent. Secondary enteric-coating was processed in organic solvent with low temperature $(below\;33^{\circ}C)$ technique, which minimized the heat labilability of Lactobacillus. Survival rate of Lactobacillus within microcapsule was not less than 95% and acid tolerance was above 30% in the artificial gastric acid. Further more it was dissolved in the artificial intestine juice within 2-3 hr. Average size of Lactobacillus microcapsules was $450\;{\mu}m$(25-50 mesh) and its viability was above 90% in the direct tableting.