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Analysis of Meteorological Elements in the Cultivated Area of Hadong Green Tea (하동녹차 재배지역의 기상요소별 분석)

  • Hwang, Jung-Gyu;Kim, Jong-Cheol;Cho, Kyoung-Hwan;Han, Jae-Yoon;Kim, Ru-Mi;Kim, Yeon-Su;Cheong, Gang-Won;Kim, Yong-Duck
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.12 no.2
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    • pp.132-142
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    • 2010
  • Characteristics of meteorological elements were analyzed at Hwagae and Agyang where are the representative areas of Hadong green tea cultivation in Korea. An automatic weather monitoring system (AWS) and a simple data log were employed to measure meteorological data such as temperature, relative humidity, precipitation, and wind direction and speed for 2009. The annual average air temperature of Hwagae and Agyang was 14.5 and 14.2, respectively, showing the warmest month in August ($25.4^{\circ}C$ for Hwagae and $24.9^{\circ}C$ for Agyang) and the coldest month in January ($0.3^{\circ}C$ for Hwagae and $0.2^{\circ}C$ for Agyang). Annual average of daily temperature difference (= daily maximum temperature - daily minimum temperature) was $11.3^{\circ}C$ for Hwagae and $11.1^{\circ}C$ for Agyang. Hwagae and Agyang had 62.7% and 65.3% of the annual average relative humidity, respectively. Annual precipitation was 1387 mm for Hwagae and 1793 mm for Agyang of which were higher of 605mm for Hwagae and 835 mm for Agyang compared to that in 2008. Majority of precipitation occurred between May and August, attributing 77.6% for Hwagae and 76.6% for Agyang to the annual precipitation. The annual total sunshine duration was 2054.3 hrs in Hwagae with the longest monthly sunshine duration in May (235.1 hrs) and the shortest monthly sunshine duration in July (102.5 hrs). Dominant wind direction changed seasonally from northwesterly wind in fall and winter to southeasterly wind in spring and summer. The annual average wind speed was 1.5 m $s^{-1}$ with the highest monthly wind speed of 2.0 m $s^{-1}$ in December and the lowest monthly wind speed of 1.1 m $s^{-1}$ in February. It is expected that continuous observation and assessment of meteorological data will improve our understanding of optimal environmental conditions for green tea cultivation and be used for developing models of green tea cultivation in the Hadong area.

Flow Resistance of Plane Nettings for Net Cages (우리 그물용 평면 그물감의 유수저항)

  • KIM Tae-Ho;KIM Dae-An;RYU Cheong-Ro
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.3
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    • pp.254-259
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    • 2001
  • In order to make clear the resistance of plane nettings u,sed widely in constructing net cages, the resistance R taken by $R=kSU^2$, where S was the wall area of nettings, U the flow velocity, and k the resistance coefficient, was measured in a circulating water channel by using nylon Raschel nettings and PE trawler-knotted nettings coated with anti-fouling paint or not and then the properties of coefficient k were investigated. The mesh size L and the angle $\phi$ between two adjacent bars was given by the function of Reynolds number ${\lambda}U/v$ in the region of ${\lambda}U/v<180$, i. e., $$k=350(\frac{\lambda U}{v})^{-0.25}$$.where $\lambda$ was the representative size of nettings expressed as $$\lambda=\frac{{\pi}d^2}{2L\;sin\;2{\phi}}$$On the other hand, the coefficient k was almost fixed between 92 and 102 ($kg{\cdot}s^2/m^4$) in the region of ${\lambda}U/v{\geq}180$ and varied according to the ratio $S_n/S$ of the total area $S_n$ of nettings projected to the plane perpendicular to the water flow to the wall area S of nettings, i.e., it was given by $$k=98.6(\frac{S_n}{S})^{1.19}$$ regardless of the coating of paint.

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Improvement of Meju Preparation Method for the Production of Korean Traditional kanjang (Soy Sauce) (한국 재래식 간장의 품질 향상을 위한 메주 제조법 개선)

  • Im, Moo-Hyeog;Choi, Jong-Dong;Chung, Hyun-Chae;Lee, Seon-Ho;Lee, Coon-Woo;Choi, Cheong;Choi, Kwang-Soo
    • Korean Journal of Food Science and Technology
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    • v.30 no.3
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    • pp.608-614
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    • 1998
  • In order to establish the scientific foundations for the production of Korean traditional kanjang (soy sauce) in a semi-pilot scale, meju preparation and kanjang mashing methods were investigated. Two types of meju, one that was prepared by conventional method (CM-meju) and the other that prepared by modified conventional method (MCM-meju), were made and compared their characteristics. The former made of cooked and crushed soybean with a brick shape was fermented at $15{\sim}20^{\circ}C\;and\;40{\sim}50%$ relative humidity (RH) for 30 days in koji room and the latter was fermented at $25{\sim}30^{\circ}C\;and\;80{\sim}90%$ RH seemingly being optimum for the growth of meju-organisms for the same period. The quality of MCM-meju as a raw material for the kanjang preparation was considered to be superior to that of CM-meju as the higher soluble nitrogen and total free amino acids content, the major factor for the evaluation of the quality of meju, were found in the former although the higher total nitrogen content and lower % weight loss were observed in the latter during meju preparation process. The quality of MCM-kanjang with higher total nitrogen, free amino acids, free sugars and the lower residual nitrogen content in cake after separation of kanjang was also found to be superior to that of CM-kanjang in sensory evaluation results.

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Application of Gamma Irradiation for the Microbiological Safety of Fried-Frozen Cheese Ball (냉동치즈볼의 미생물학적 안전성 확보를 위한 감마선 조사기술의 이용)

  • Lee, Ju-Woon;Kim, Jae-Hun;Kim, Jang-Ho;Oh, Sang-Hee;Seo, Ji-Hyun;Kim, Cheon-Jei;Cheong, Sung-Hee;Byun, Myung-Woo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.5
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    • pp.729-733
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    • 2005
  • This study was conducted to sanitize fried-frozen cheese ball by gamma irradiation. Total aerobic bacteria and yeasts and molds counts were 4.4 and 2.8 log CFU/g in non-irradiated sample, respectively. Microorganisms were decreased with increase of irradiation dose $(D_{10}=1.25\;kGy)$, and were not detected in samples irradiated at 3 kGy or more $(<10^2\;CFU/g)$. $D_{10}$ value for Escherichia coli (KCTC 1682) was 0.25 kGy. TBA (2-thiobarbituric acid) values were increased as irradiation dose was increased, but there was no significant difference between non-irradiated and irradiated samples at 3 kGy or less (p<0.05). The results of sensory evaluation showed that there was no significant difference between non-irradiated and irradiated samples at 3 kGy or less, the sensory scores were decreased with irradiation at 5 kGy or more (p<0.05). These results indicated that gamma irradiation at 3 kGy was considered to be an effective treatment to ensure the microbiological safety of fried-frozen cheese balls without any sensorial change, even though further studies should be investigated to reduce detrimental effects induced by irradiation.

Studies on Change of Lipids Improvement-Meju during the Fermentation (개량(改良)메주의 숙성과정(熟成過程) 중 지질(脂質)조성의 변화(變化)에 관(關)한 연구(硏究))

  • Yang, Soo Dong;Bae, Man Jong;Yoon, Sang Hong;Choi, Cheong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.12 no.3
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    • pp.189-195
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    • 1983
  • Changes of lipid composition in the Improvement-Meju inoculated with Aspergillus oryzae were examined. To investigate those changes systematically, silicic acid column chromatography was used for analysis of glycolipid, neutral lipid, phospholipid, and gas chromatography to examine the change of those fatty acid content. Following results were obtained. The lipid fraction obtained from soaked soybean and cooked soybean were mainly composed of about 93~94% neutral lipid, whereas phospholipid and glycolipid was 4.0~5.0%, 2.0~2.1% level, respectively. During meju incuvation period, neutral lipid decreased gradually, but glycolipid and phospholipid increased. Among the nonpolar lipids prepared from cooked soybean and soaked soybean, triglyceride content was mainly composed of 88~89%, and the content of sterol ester, free fatty acid, diglyceride and sterol was higher in soaked soybean than in cooked soybean. During meju incuvation period, triglyceride content decreased remarkablely, whereas content of sterol ester, free fatty acid and diglyceride increased gradually. From the soaked soybean and the cooked soybean, the fatty acids content of crude lipid, neutral lipid, glycolipid and phospholipid were composed of linoleic acid 54~70%, oleic acid 20.0~22.6%, palmitic acid 11.0~12.4%, linolenic acid 6.0~7.8% and stearic acid 3.4~4.3% in turn and myristic acid showed the trace, palmitic acid was a little higher in glycolipid and phospholipid than in crude lipid and neutral lipid. During meju incuvation period, the change of fatty acid content showed linoleic acid and linolenic acid reduction gradually in the neutral lipid, glycolipid and phospholipid. On the other hand, palmitic acid, stearic acid and oleic acid increased gradually, the maximum value was at the 4-days. The change of glycolipid fatty acid and phospholipid fatty acid was examined. 9-kinds including traced 3-kinds was detected. It was supposed that traced 2-kinks was occurred for incuvation, and those are the matter investigating in the future.

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Effects of Simulated Acid Rain on the Chlorophyll Contents in the Needles of Pinus koraiensis and Ligustrum obtusifolium Seedlings (인공산성우(人工酸性雨)가 잣나무 및 쥐똥나무 유묘(幼苗)의 엽내(葉內) 엽록소(葉綠素) 함량(含量)에 미치는 영향(影響))

  • Kim, Chang Ho;Cheong, Yong Moon
    • Journal of Korean Society of Forest Science
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    • v.76 no.1
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    • pp.11-16
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    • 1987
  • With the purpose of pursuing the increase and decrease of chlorophyll a and chlorophyll b caused by application of the simulated acid rain treatment on Pinus koraiensis seedlings and Ligustrum obtusifolium cuttings, the experimental design of randomized block arrangement with three replications was implemented in the experimental field of Yesan National Agricultural Junior College, during the growing season of 1985. Pinus koraiensis seeds stratified in cool and moist condition were sown on pots, and in case of Ligustrum obtusifolium, C1/1 cuttings were potted for experimental use in the early spring. The regime of artificial acid rain, based upon precipitation frequency and density, was simulated from the learning of climatological data averaged from 30 years records. The spray of acid water containing pH values of 4.0 and 2.0 was initiated from the 1st of May and ended on the 31st of August. As control, ground water was also treated at the same time. To analyse the chlorophyll content, those leaves looking representative and unaffected by other harmful agents were sampled on the 18th of September, and UV-visible spectrophotometer was used. With decrease in pH values of acid rain, the content of chlorophyll a and chlorophyll b decreased in both species. The decrease in chlorophyll a could be confirmed through statistical significance, but not in chlorophyll b. And when we discussed the chlorophyll decrease index which was explained in detail in the paper, an attention might be given to similarly decreasing values in both chlorophyll a and chlorophyll b, according as pH levels of acid rain decreased. The ratios of chlorophyll a to chlorophyll b in both species were not affected by different pH leaves of acid rain.

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A Study on the Demander's Consciousness of the National Forest Management System (국유림 경영제도에 대한 수요자의 인식정도에 관한 연구)

  • Park, Kyung-Seok;Lee, Seong-Youn;Choi, Soo-Im;Kim, Hyun-Sik;Jeong, Se-Myong;An, Ki-Wan
    • Journal of Korean Society of Forest Science
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    • v.99 no.3
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    • pp.380-390
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    • 2010
  • This research was conducted to investigate the consciousness of demander on National Forest Management System and to seek for solution to activate it by measuring the variables such as the extent of goal achievement on the system, the extent of importance on function of national forest, benefit for participation of the system, and the extent of importance on the system as well as the extent of demander recognition and intention of participation on the system like, Cooperative Forest Program and People's Forest Program. The target of this investigation is to categorize consumers of National Forest Management System (NFMS) into four types such as environmental organization that do not participate in the system, environmental organization that participate in the team, company to publish a white paper on environmental and local government. As a result, the intention of participation was showed in C Type (62.7%), among fields that respondents interested in participation were tree planting and forest tending (56.2%). However, the rest of the participants were reluctant participate in the NFMS due to more benefit (72.2%) of corporate social responsibility from other fields than those from the field utilizing forestry. If only, diverse facilities related to national forest and active supporting policy are provided by Korea Forest Service, social participation using forest resources would be considered. Although 61% among A Type recognized NFMS in advance, the prime reason for not utilizing are the problems with reserving place for participates in and the inconvenience to participate in on-site. Type D shows slightly high interest in People's Forest Program (3.69). Also, it shows high interest in Forest Recreation (4.15) and Forest Reports (3.90). Particularly, it indicates that GyeongGi-Do and GangWon-Do local government prefers Forest Experience, and Cheolla, ChungCheong and GyeongSang local government prefer Forest Reports. Based on the above study, we finally suggest that legal alignment of the system, and the provision of incentive for inducement of voluntary participation by Corporate Social Responsibility (CSR), and establishment of new exclusive nongovernmental organization be able to operate the system as the solution to activate NFMS in terms of the consumer.

A Study on the Distributional Characteristics of Unminable Manganese Nodule Area from the Investigation of Seafloor Photographs (해저면 영상 관찰을 통한 망간단괴 채광 장애지역 분포 특성 연구)

  • Kim, Hyun-Sub;Jung, Mee-Sook;Park, Cheong-Kee;Ko, Young-Tak
    • Geophysics and Geophysical Exploration
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    • v.10 no.3
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    • pp.173-182
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    • 2007
  • It is well known that manganese nodules enriched with valuable metals are abundantly distributed in the abyssal plain area in the Clarion-Clipperton (C-C) fracture zone of the northeast Pacific. Previous studies using deep-sea camera (DSC) system reported different observations about the relation of seafloor topographic change and nodule abundance, and they were sometimes contradictory. Moreover, proper foundation on the estimation of DSC underwater position, was not introduced clearly. The variability of the mining condition of manganese nodule according to seafloor topography was examined in the Korea Deep Ocean Study (KODOS) area, located in the C-C zone. In this paper, it is suggested that the utilization of deep towing system such as DSC is very useful approach to whom are interested in analysing the distributional characteristics of manganese nodule filed and in selecting promising minable area. To this purpose, nodule abundance and detailed bathymetry were acquired using deep-sea camera system and multi-beam echo sounder, respectively on the seamount free abyssal hill area of southern part ($132^{\circ}10'W$, $9^{\circ}45'N$) in KODOS regime. Some reasonable assumptions were introduced to enhance the accuracy of estimated DSC sampling position. The accuracy in the result of estimated underwater position was verified indirectly through the comparison of measured abundances on the crossing point of neighboring DSC tracks. From the recorded seafloor images, not only nodules and sediments but cracks and cliffs could be also found frequently. The positions of these probable unminable area were calculated by use of the recorded time being encountered with them from the seafloor images of DSC. The results suggest that the unminable areas are mostly distributed on the slope sides and hill tops, where nodule collector can not travel over.

Protective Effects on A2Kb Transgenic Mice That Were Immunized with Hepatitis B Virus X Antigen Peptides by the Activation of CD8+ T Cells; XEP-3 Specific CTL Responses in the in vitro Culture (B형 간염 바이러스 X 항원을 면역한 A2Kb Transgenic Mice에서 CD8+ T Cell의 활성화에 의한 X 항원 표현 재조합 Vaccinia Virus에 대한 방어 효과; in vitro 배양을 통한 XEP-3 특이적인 CTL의 반응)

  • Hwang, Yu Kyeong;Kim, Hyung-Il;Kim, Nam Kyung;Park, Jung Min;Cheong, Hong Seok
    • IMMUNE NETWORK
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    • v.2 no.1
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    • pp.41-48
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    • 2002
  • Background: Viral antigens presented on the cell surface in association with MHC class I molecules are recognized by CD8+ T cells. MHC restricted peptides are important in eliciting cellular immune responses. As peptide antigens have a weak immunigenicity, pH-sensitive liposomes were used for peptide delivery to induce effective cytotoxic T lymphocyte (CTL) responses. In the previous study, as the HBx peptides could induce specific CTLs in vitro, we tested whether the HLA-A2/$K^b$ transgenic mice that were immunized by HBx-derived peptides could be protected from a viral challenge. Methods: HBx-peptides encapsulated by pH-sensitive liposomes were prepared. $A2K^b$ transgenic mice were immunized i.m. on days one and seven with the indicated concentrations of liposome-encapsulated peptides. Three weeks later, mice were infected with $1{\times}10^7pfu$/head of recombinant vaccinia virus (rVV)-HBx via i.p. administration. The ovaries were extracted from the mice, and the presence of rVV-HBx in the ovaries was analyzed using human TK-143B cells. IFN-${\gamma}$ secretion by these cells was directly assessed using a peptide-pulsed target cell stimulation assay with either peptide-pulsed antigen presenting cells (APCs), concanavalin A ($2{\mu}g/ml$), or a vehicle. To generate peptide-specific CTLs, splenocytes obtained from the immunized mice were stimulated with $20{\mu}g/ml$ of each peptide and restimulated with peptide-pulsed APC four times. The cytotoxic activity of the CTLs was assessed by standard $^{51}Cr$-release assay and intracellular IFN-${\gamma}$ assay. Results: Immunization of these peptides as a mixture in pH-sensitive liposomes to transgenic mice induced a good protective effect from a viral challenge by inducing the peptide-specific CD8+ T cells. Mice immunized with $50{\mu}g/head$ were much better protected against viral challenge compared to those immunized with $5{\mu}g$/head, whereas the mice immunized with empty liposomes were not protected at all. After in vitro CTL culture by peptide stimulation, however, specific cytotoxicity was much higher in the CTLs from mice immunized with $5{\mu}g/head$ than $50{\mu}g/head$ group. Increase of the number of cells that intracellular IFN-${\gamma}$ secreting cell among CD8+ T cells showed similar result. Conclusion: Mice immunized with XEPs within pH-sensitive liposome were protected against viral challenge. The protective effect depended on the amount of antigen used during immunization. XEP-3-specific CTLs could be induced by peptide stimulation in vitro from splenocytes obtained from immunized mice. The cytotoxic effect of CTLs was measured by $^{51}Cr$-release assay and the percentage of accumulated intracellular IFN-${\gamma}$ secreting cells after in vitro restimulation was measured by flow cytometric analysis. The result of $^{51}Cr$-release cytotoxicity test was well correlated with that of the flow cytometric analysis. Viral protection was effective in immunized group of $50{\mu}g/head$, while in the in vitro restimulation, it showed more spectific response in $5{\mu}g$/head group.

Finite element analysis of peri-implant bone stress influenced by cervical module configuration of endosseous implant (임플란트 경부형상이 주위골 응력에 미치는 영향에 관한 유한요소법적 분석)

  • Chung, Jae-Min;Jo, Kwang-Heon;Lee, Cheong-Hee;Yu, Won-Jae;Lee, Kyu-Bok
    • The Journal of Korean Academy of Prosthodontics
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    • v.47 no.4
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    • pp.394-405
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    • 2009
  • Statement of problem: Crestal bone loss, a common problem associated with dental implant, has been attributed to excessive bone stresses. Design of implant's transgingival (TG) part may affect the crestal bone stresses. Purpose: To investigate if concavely designed geometry at a dental implant's TG part reduces peri-implant bone stresses. Material and methods: A total of five differently configured TG parts were compared. Base model was the ITI one piece implant (Straumann, Waldenburg, Switzerland) characterized by straight TG part. Other 4 experimental models, i.e. Model-1 to Model-4, were designed to have concave TG part. Finite element analyses were carried out using an axisymmetric assumption. A vertical load of 50 N or an oblique load of 50 N acting at $30^{\circ}$ with the implant's long axis was applied. For a systematic stress comparison, a total of 19 reference points were defined on nodal points around the implant. The peak crestal bone stress acting at the intersection of implant and crestal bone was estimated using regression analysis from the stress results obtained at 5 reference points defined along the mid plane of the crestal bone. Results: Base Model with straight configuration at the transgingival part created highest stresses on the crestal bone. Stress level was reduced when concavity was imposed. The greater the concavity and the closer the concavity to the crestal bone level, the less the crestal stresses. Conclusion: The transgingival part of dental implant affect the crestal bone stress. And that concavely designed one may be used to reduce bone stress.