• Journal of fish pathology
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• v.17 no.3
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• pp.217-222
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• 2004

This study was performed to investigate the immunological side effects of chloramphenicol (CAP) on olive flounder, Paralichthys olivaceus. To investigate immunological effects on olive flounder, we determined the changes of chemiluminescence (CL) response of flounder kidney-derived leucocyte after the treatment of CAP in vivo and in vitro. The CL activity was significantly decreased in a dose-dependent manner during the treatment of CAP in vitro. Similarly, a dose-dependent reduction of CL response, although not significant, were observed during the treatment of CAP in vivo. The results suggest that CAP reduced the function of flounder phagocytosis in vivo and in vitro, indicating the immunosuppressive ability of CAP.

• Nuclear Engineering and Technology
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• v.33 no.6
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• pp.626-637
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• 2001

A series of study on energy dependence in chemiluminescence dosimetry with sugar and sorbite produced in two different countries was carried out administering a dose of 5 Gy to the samples at six different mean photon energies of 30, 50, 80, 130, 662 and 1250 keV. The results revealed distinct energy dependence of chemiluminescence(CL) output of sugar and sorbite. Although the energy dependence, in general, could be fitted by a polynomial of (os E, with I being radiation energy, up to cubic term, we reached a conclusion that the adoption of a fitting function, $y_{R}$$^{=a}$.(1-e $^{-b.logE}$)$^{c}$ +d, deduced from theoretical energy response curve calculated as the ratio of the mass energy absorption of the samples of interest to the soft tissue is more reasonable and rational. Herecoefficients $y_{R}$ is CL intensity, and a, b, c and d are constants to be determined in the fitting process. Energy dependence of relative sensitivities of one sample to the other, discrepancy in sensitivities of the samples from the two countries, and prominent grain size effect in Sorbitol were also shown.shown.

• Journal of fish pathology
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• v.14 no.2
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• pp.103-105
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• 2001

The effect of opsonization of zymosan with serum on the repiratory burst of haemocytes isolated from the Pacific oyster Crassostrea gigas was investigated. The reactive oxygen species (ROS) produced by the respiratory burst of haemocytes in response to the opsonized or unopsonized zymosan were measured using chemiluminescence (CL). The CL values were increased according to the increment of haemocyte number. The degree of CL amplification by increase of haemocytes from $0.1{\times}10^6$ to $0.5{\times}10^6$ cells/ml was 3-5 times, but comparatively low amplification was elicited by increase of haemocytes from $0.5{\times}10^6$ to $1{\times}10^6$ cells/ml. Chemiluminescences produced by the haemocytes in response to the zymosan opsonized with oyster serum were considerably higher than the CL produced by the haemocytes in response to the unopsonized zymosan.

• Bulletin of the Korean Chemical Society
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• v.30 no.10
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• pp.2387-2392
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• 2009

A composite film of multi-walled carbon nanotube (MWCNT)/sol-gel-derived zinc oxide(ZnO)/Nafion has been utilized as an efficient immobilization matrix for the construction of a highly sensitive and stable tris(2,2'-bipyridyl) ruthenium(II) (Ru(${bpy)_3}^{2+})$ electrogenerated chemiluminescence (ECL) sensor. The electrochemical and ECL behaviors of Ru(${bpy)_3}^{2+})$ ion-exchanged into the composite film were strongly dependent upon the sol-gel preparation condition, the amount of MWCNT incorporated into the ZnO/Nafion composite film, and the buffer solution pH. The synergistic effect of MWCNTs and ZnO in the composite films increased not only the sensitivity but also the long-term stability of the ECL sensor. The present ECL sensor based on the MWCNT/ZnO/Nafion gave a linear response ($R^2$ = 0.999) for tripropylamine concentration from 500 nM to 1.0 mM with a remarkable detection limit (S/N = 3) of 15 nM. The present ECL sensor showed outstanding long-term stability (94% initial signal retained for 5 weeks). Since the present ECL sensor exhibits large response towards NADH, it could be applied as a transduction platform for the ECL biosensor in which the NADH is produced from the dehydrogenase-based enzymatic reaction in the presence of NA$D^+$ cofactor.

• Journal of fish pathology
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• v.32 no.1
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• pp.21-28
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• 2019

It has been reported that various anti-oxidant substances stimulate non-specific immune responses in fishes. In this study it was examined whether N-acetylcysteine (NAC), a precusor for anti-oxidant glutathione (GSH) synthesis, can modulate non-specific immune function in Far Eastern catfish Silurus asotus. Immune functions were assessed using the respiratory burst activity monitored by chemiluminescence (CL) responses in isolated leucocyte. NAC stimulated CL responses with doses of 10 or 100 mg/kg, but not with 1 mg/kg after 48 hr injection. It was observed with 10 mg/kg NAC that CL activity continued to elevate from 24 hr through 96 hr post-dosing, and returned to the near preinjection level by 10 days. To understand whether NAC can also activate CL activity in vitro, NAC was directly added to isolated catfish leucocytes. It was observed, however, that NAC can not stimulate CL at reasonable concentration ranges in vitro. As NAC is a precursor for the strong anti-oxidant glutathione (GSH), a putative immune stimulator, it was assessed whether GSH can also stimulate CL responses. Observed results show that GSH activated CL both in vivo and in vitro. The data obtained collectively support the proposition that NAC indirectly stimulates non-specific immune functions in catfish by enhancing GSH biosynthesis, but not by direct action of NAC. Such effects may have beneficial significance in aquaculture for practical utilization.

• 한국연소학회:학술대회논문집
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• 2000.05a
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• pp.142-151
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• 2000

Dump combustor is a combustor having a dump plane to make coherent structures. A non-premixed flame dump combustor of simple geometry was constructed. We conducted basic experiments such as frequency response on the combustor to confirm the characteristics of the phenomena as a typical dump combustion and unsteady combustion. Furthermore we visualized the flame front behavior by CH chemiluminescence and high speed motion analysis. In spite of the lack of another data such as velocity, species concentration and temperature, the results showed not only the periodic motion of flame front but the ignition process of vortex ring flame. Also we could check out Rayleigh criterion by combining the visualization data with the pressure data.

• Journal of the Korean Society of Combustion
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• v.14 no.4
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• pp.48-53
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• 2009

This paper describes the forced flame response in a turbulent premixed gas turbine combustor. The fuel was premixed with the air upstream of a choked inlet to avoid equivalence ratio fluctuations. To impose the inlet flow velocity, a siren type modulation device was developed using an AC motor, rotating and static plates. Measurements were made of the velocity fluctuation in the nozzle using hot wire anemometry and of the heat release fluctuation in the combustor using chemiluminescence emission. The test results showed that flame length as well as geometry was strongly dependent upon modulation frequency in addition to operating conditions such as inlet velocity. Convection delay time between the velocity perturbation and heat release fluctuations was calculated using phase information of the transfer function, which agreed well with the results of flame length measurements. Also, basic characteristics of the flame nonlinear response shown in the current test conditions were introduced.

• Journal of the Korean Society of Combustion
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• v.22 no.2
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• pp.19-26
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• 2017

An experimental study investigates the flame response characteristics of jet-diffusion flame and premixed flame. The experiment was conducted while varying the amplitude. Flame lengths were quantified for OH chemiluminescence measurement and compared with the result of the flame transfer function. Flame length and flame velocity perturbation were normalized and compared with the result of the flame transfer function. The comparison results appear that velocity perturbation and flame length oscillation of premixed flame show linear behaviors on the other hand jet-diffusion flame, amplitudes are more thant 0.20, shows nonlinear behaviors of flame velocity perturbation and flame length oscillation.

• Journal of the Korean Society of Combustion
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• v.13 no.2
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• pp.1-6
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• 2008

An experimental study of the flame response in a turbulent premixed combustor has been conducted with room temperature, atmospheric pressure inlet conditions using premixed natural gas. The fuel is premixed with the air upstream of a choked inlet to avoid equivalence ratio fluctuations. Therefore the observed flame response is only the result of the imposed velocity fluctuations, which are produced using a variable speed siren. Measurements are made of the velocity fluctuation in the nozzle using hot wire anemometry and of the heat release fluctuation in the combustor using chemiluminescence emission. The results are analyzed to determine the phase and gain of the flame transfer function as a function of the modulation frequency. Of particular interest is the effect of flame structure on the flame response predictions and measurements. The results show that both the gain and the phase of flame transfer function are closely associated with the flame length and structure, which is dependent upon the upstream flow perturbation as well as equivalence ratio in the current study.

• The Journal of Pediatrics of Korean Medicine
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• v.15 no.2
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• pp.15-30
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• 2001

The purpose of this research was to investigate the effects of BITG on immune response in the young mice. BITG(500mg/kg) was administerd per orally once a day for 7 days to the young BALB/C(4 wks old)mice. The administrationo of BITG enhanced the cell viability of splenocytes, but did not affect that of thymocytes. In vitro system, BITG decreased the cell viability of thymocytes and splenocytes at the concentration of $100{\mu}g/m{\ell}$. The administration of BITG did not affect DNA fragmentation of thymocytes, but decreased that of splenocytes. The administration of BITG increased the population of Thy1+ cell and CD4+CD8- cell in splenocytes. In addition, BITG decreased the production of NO and increased the lucigenin chemiluminescence from peritoneal macrophages. These results suggest that BITG enhances the specific-immune and nonspecific-immune response via increase of cell viability in splenocytes and of phagocytic activity in peritoneal macrophages.