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http://dx.doi.org/10.7847/jfp.2019.32.1.021

Effects of N-acetylcysteine (NAC) on chemiluminescence response as a non-specific immune parameter in Far Eastern catfish Silurus asotus  

Park, Kwan Ha (Departments of Aquatic Life Medicine, Kunsan National University)
Lee, Han-Na (Departments of Aquatic Life Medicine, Kunsan National University)
An, Jae-Young (Departments of Aquatic Life Medicine, Kunsan National University)
Bae, Jun Sung (Departments of Aquatic Life Medicine, Kunsan National University)
Lee, Chae Won (Departments of Aquatic Life Medicine, Kunsan National University)
Yang, Chan Young (Departments of Aquatic Life Medicine, Kunsan National University)
Choi, Sang-Hoon (Departments of Aquatic Life Medicine, Kunsan National University)
Publication Information
Journal of fish pathology / v.32, no.1, 2019 , pp. 21-28 More about this Journal
Abstract
It has been reported that various anti-oxidant substances stimulate non-specific immune responses in fishes. In this study it was examined whether N-acetylcysteine (NAC), a precusor for anti-oxidant glutathione (GSH) synthesis, can modulate non-specific immune function in Far Eastern catfish Silurus asotus. Immune functions were assessed using the respiratory burst activity monitored by chemiluminescence (CL) responses in isolated leucocyte. NAC stimulated CL responses with doses of 10 or 100 mg/kg, but not with 1 mg/kg after 48 hr injection. It was observed with 10 mg/kg NAC that CL activity continued to elevate from 24 hr through 96 hr post-dosing, and returned to the near preinjection level by 10 days. To understand whether NAC can also activate CL activity in vitro, NAC was directly added to isolated catfish leucocytes. It was observed, however, that NAC can not stimulate CL at reasonable concentration ranges in vitro. As NAC is a precursor for the strong anti-oxidant glutathione (GSH), a putative immune stimulator, it was assessed whether GSH can also stimulate CL responses. Observed results show that GSH activated CL both in vivo and in vitro. The data obtained collectively support the proposition that NAC indirectly stimulates non-specific immune functions in catfish by enhancing GSH biosynthesis, but not by direct action of NAC. Such effects may have beneficial significance in aquaculture for practical utilization.
Keywords
N-acetylcysteine (NAC); Non-specific immune functions; Chemiluminescence (CL) response; Far Eastern catfish;
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