Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Effects of N-acetylcysteine (NAC) on chemiluminescence response as a non-specific immune parameter in Far Eastern catfish Silurus asotus

항산화물질 N-acetylcysteine (NAC)이 메기에서 비특이적 면역지표인 화학발광 반응에 미치는 영향

  • Park, Kwan Ha (Departments of Aquatic Life Medicine, Kunsan National University) ;
  • Lee, Han-Na (Departments of Aquatic Life Medicine, Kunsan National University) ;
  • An, Jae-Young (Departments of Aquatic Life Medicine, Kunsan National University) ;
  • Bae, Jun Sung (Departments of Aquatic Life Medicine, Kunsan National University) ;
  • Lee, Chae Won (Departments of Aquatic Life Medicine, Kunsan National University) ;
  • Yang, Chan Young (Departments of Aquatic Life Medicine, Kunsan National University) ;
  • Choi, Sang-Hoon (Departments of Aquatic Life Medicine, Kunsan National University)
  • 박관하 (군산대학교 해양과학대학 해양생명의학과) ;
  • 이한나 (군산대학교 해양과학대학 해양생명의학과) ;
  • 안재영 (군산대학교 해양과학대학 해양생명의학과) ;
  • 배준성 (군산대학교 해양과학대학 해양생명의학과) ;
  • 이채원 (군산대학교 해양과학대학 해양생명의학과) ;
  • 양찬영 (군산대학교 해양과학대학 해양생명의학과) ;
  • 최상훈 (군산대학교 해양과학대학 해양생명의학과)
  • Received : 2019.06.06
  • Accepted : 2019.06.18
  • Published : 2019.06.30
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Abstract

It has been reported that various anti-oxidant substances stimulate non-specific immune responses in fishes. In this study it was examined whether N-acetylcysteine (NAC), a precusor for anti-oxidant glutathione (GSH) synthesis, can modulate non-specific immune function in Far Eastern catfish Silurus asotus. Immune functions were assessed using the respiratory burst activity monitored by chemiluminescence (CL) responses in isolated leucocyte. NAC stimulated CL responses with doses of 10 or 100 mg/kg, but not with 1 mg/kg after 48 hr injection. It was observed with 10 mg/kg NAC that CL activity continued to elevate from 24 hr through 96 hr post-dosing, and returned to the near preinjection level by 10 days. To understand whether NAC can also activate CL activity in vitro, NAC was directly added to isolated catfish leucocytes. It was observed, however, that NAC can not stimulate CL at reasonable concentration ranges in vitro. As NAC is a precursor for the strong anti-oxidant glutathione (GSH), a putative immune stimulator, it was assessed whether GSH can also stimulate CL responses. Observed results show that GSH activated CL both in vivo and in vitro. The data obtained collectively support the proposition that NAC indirectly stimulates non-specific immune functions in catfish by enhancing GSH biosynthesis, but not by direct action of NAC. Such effects may have beneficial significance in aquaculture for practical utilization.

Keywords

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Fig. 1. Effects of different N-acetylcysteine (NAC) doses on chemiluminesence (CL) responses in isolated catfish leucocytes after intraperitoneal injection. CL activity was examined 48 hr after NAC injections. CL was expressed as peak responses obtained from 40 min continuous monitoring. Mean ± S.D. of 6 fish for each group. *Significantly different from control at p < 0.05 with Newman-Keuls t-test.

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Fig. 2. Effects of different treatment period of N-acetylcysteine (NAC) on chemiluminesence (CL) responses in isolated catfish leucocytes after intraperitoneal injection. CL activity was examined 48 hr after NAC injections. CL was expressed as peak responses obtained from 40 min continuous monitoring. Mean ± S.D. of 6 fish for each group. *Significantly different from control at p < 0.05 with Newman-Keuls t-test.

HGOPB8_2019_v32n1_21_f0003.png 이미지

Fig. 3. In vitro effects of N-acetylcysteine (NAC) on chemiluminesence (CL) responses in isolated catfish leucocytes. CL was expressed as peak responses obtained from 40 min continuous monitoring. Mean ± S.D. of N=10 replicates. *Significantly different from control at p < 0.05 with Newman-Keuls t-test.

HGOPB8_2019_v32n1_21_f0004.png 이미지

Fig. 4. In vivo (a) and in vitro (b) effects of glutathione (GSH) on chemiluminesence (CL) responses in isolated catfish leucocytes. GSH was injected intraperitoneally 48 hr before CL response assessment for in vivo experiment (a), while GSH was added directly into isolated leucocyles for in vitro tests (b). CL was expressed as peak responses obtained from 40 min continuous monitoring. Mean ± S.D. of 6 fish for in vivo and 10 replicates for in vitro. *Significantly different from control at p < 0.05 with Newman-Keuls t-test.

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