• Korean Journal of Environmental Biology
• /
• v.21 no.1
• /
• pp.52-55
• /
• 2003

There is considerable evidence that ionizing radiation (IR) mediates checkpoint control, repair and cell death. In this study, we have used a high density microarray hybridization approach to characterize the transcriptional response of human breast carcinoma MCF-7 cell line to ${\gamma}$-radiation, such as 4 Gy 4 hr, 8 Gy 4 hr, and 8 Gy 12 hr. We found that exposure to ${\gamma}$-ray alters by at least a $log_2$ factor of 1.0 the expression of 115 known genes. Of the 66 genes affected by ${\gamma}$-radiation, 49 are down-regulated. In our results, the cellular response to irradiation includes induction of the c-jun and EGR1 early response genes. The present work has examined potential cytoplasmic signaling cascades that transduce IR-induced signals to the nucleus. 40S ribosomal protein s6 kinase modulates the activities of the mitogen activated protein kinase (MAPK) and c-Jun $NH_2$-terminal kinase (JNK1) cascades in human monocytic leukemia (U937/pREP4) cells. 14-3-3 family members are dimeric phosphoserine -binding proteins that participate in signal transduction and checkpoint control pathways.

• YAKHAK HOEJI
• /
• v.46 no.1
• /
• pp.18-23
• /
• 2002

Ailanthus altissima has been used to settle an upset stomach, to alleviate a fever and as an insecticide. We reported that the water extract of A. altissima induced apoptotic cell death in Jurkat T-acute Iymphoblastic leukemia cells. Here, we showed the dose-dependent inhibitions of cell viability by the extract, as measured by cell morphology. The cell cycle control genes are considered to play important roles in tumorigenesis. The purpose of the present study is also to investigate the effect of A. altissima on cell cycle progression and its molecular mechanism in the cells. The level of p21 protein was increased after treatment of the extract, whereas both Bcl-2 and Bax protein levels were not changed. These results suggest that A. altissima induces apoptotic cell death via p21-dependent signaling pathway in Jurkat cells which delete wild type p53. Gl checkpoint related gene products tested (cyclin D3, cyclin dependent kinase 4, retinoblastoma, E2Fl) were decreased in their protein levels in a dose-dependent manner after treatment of the extract Taken together, these results indicate that the increase of apoptotic cell death by A. altissima may be due to the inhibition of cell cycle in Jurkat cells.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.27 no.1B
• /
• pp.13-23
• /
• 2002

A mobile host is prone to failure due to lack of stable storage, low bandwidth of wireless channel, high mobility, and limited battery life on the wireless network. Many researchers have studied to overcome these problems. For high level Availability in the cellular networks, it is necessary to consider recovery from the failures of mobile support stations as well as mobile as mobile hosts. In this paper, we present modified trickle scheme for recovery from failures of Mobile Support Station based on checkpointing scheme and analyze and compare the performance. We propose and analyze the performance of two schemes : one is waiting recovery scheme for the mobile support station having the last checkpoint and the other is searching the new path to the another mobile support station having the checkpoint.

• Asian Pacific Journal of Cancer Prevention
• /
• v.17 no.3
• /
• pp.905-910
• /
• 2016

Breast cancer is one of the major threats to female health, and its incidence is rapidly increasing in many countries. Currently, breast cancer is treated with surgery, followed by chemotherapy or radiation therapy, or both. However, a substantial proportion of breast cancer patients might have a risk for local relapse that leads to recurrence of their disease and/or metastatic breast cancer. Therefore searching for new and potential strategies for breast cancer treatment remains necessary. Immunotherapy is an attractive and promising approach that can exploit the ability of the immune system to identify and destroy tumors and thus prevent recurrence and metastatic lesions. The most promising and attractive approach of immunotherapeutic research in cancer is the blockade of immune checkpoints. In this review, we discuss the potential of certain inhibitors of immune checkpoints, such as antibodies targeting cytotoxic T-lymphocyte antigen 4 (CTLA-4), programmed death 1 (PD-1) and lymphocyte activation gene-3 (LAG-3), in breast cancer therapeutics. Immune checkpoint inhibitors may represent future standards of care for breast cancer as monotherapy or combined with standard therapies.

• Korean Journal of Pharmacognosy
• /
• v.33 no.1 s.128
• /
• pp.29-34
• /
• 2002

Albizzia julibrissin belonging to the family Leguminosae has been used for the treatment of contusion, sore throat, amnesia, and insomnia in Oriental traditional medicine. The water extract of A. julibrissin induced apoptosis in Jurkat T-acute lymphoblastic leukemia (ALL) cells as measured by cell morphology. The capability of this herb medicine to induce apoptosis was associated with proteolytic cleavage of specific target protein such as beta-catenin protein suggesting the possible involvement of caspases. The purpose of the present study is also to investigate the effect of A. julibrissin on cell cycle progression. Our results showed that GI checkpoint related gene products (cyclin D1, cyclin dependent kinase 4, retinoblastoma, E2F1) were decreased in their protein levels in a dose-dependent manners after treatment of the extract. These results indicate that the increase of apoptotic cell death by A. julibrissin may be due to the inhibition of cell cycle progression in wild type p53-lacking Jurkat cells.

• Korean Journal of Head & Neck Oncology
• /
• v.33 no.1
• /
• pp.1-5
• /
• 2017

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer globally with high morbidity and mortality. Immune surveillance is well recognized as an important mechanism to prevent development or progression of HNSCC. HNSCC can escape the immune system through multiple mechanisms including development of tolerance in T cells and inhibition of T-cell-related pathways, generally referred to as checkpoint inhibitors. Recent clinical trials have demonstrated a clear advantage in advanced HNSCC patients treated with immune checkpoint blockade. Right at the front of the new era of immunotherapy, we will review current knowledge of immune escape mechanisms and clinical implication for HNSCC.

• Radiation Oncology Journal
• /
• v.38 no.1
• /
• pp.1-10
• /
• 2020

Radiotherapy (RT) has been used for decades as one of the main treatment modalities for cancer patients. The therapeutic effect of RT has been primarily ascribed to DNA damage leading to tumor cell death. Besides direct tumoricidal effect, RT affects antitumor responses through immune-mediated mechanism, which provides a rationale for combining RT and immunotherapy for cancer treatment. Thus far, for the combined treatment with RT, numerous studies have focused on the immune checkpoint inhibitors and have shown promising results. However, treatment resistance is still common, and one of the main resistance mechanisms is thought to be due to the immunosuppressive tumor microenvironment where myeloid-derived suppressor cells (MDSCs) play a crucial role. MDSCs are immature myeloid cells with a strong immunosuppressive activity. MDSC frequency is correlated with tumor progression, recurrence, negative clinical outcome, and reduced efficacy of immunotherapy. Therefore, increasing efforts to target MDSCs have been made to overcome the resistance in cancer treatments. In this review, we focus on the role of MDSCs in RT and highlight growing evidence for targeting MDSCs in combination with RT to improve cancer treatment.

• Journal of the Institute of Electronics Engineers of Korea TC
• /
• v.44 no.7 s.361
• /
• pp.112-121
• /
• 2007

In this paper, we propose a new checkpointing strategy for dual modular redundancy real-time systems. For every checkpoints the execution results from two processors, and the result saved in the previous checkpoint are compared to detect faults. We devised an operation algorithm in chectpoints to recover from transient faults as well as permanent faults. We also develop a Markov model for the optimization of the proposed checkpointing strategy. The probability of successful task execution within its deadline is derived from the Markov model. The optimal number of checkpoints is the checkpoints which makes the successful probability maximum.

• The KIPS Transactions:PartA
• /
• v.19A no.3
• /
• pp.113-120
• /
• 2012

The visibility for signals in designs is required for their analysis and debug during the verification process. It could be achieved through the signal dumping for designs during the execution of HDL simulation. However, such signal dumping, in general, degrades the speed of simulation significantly, or can result in the number of simulation runs. In this paper, we have proposed an efficient and fast simulation method for dumping based on the design checkpoint, and shown its effectiveness by applying it to industrial SOC designs.

• The Transactions of the Korea Information Processing Society
• /
• v.6 no.9
• /
• pp.2533-2539
• /
• 1999

Two scheme are widely used for fault-tolerant systems : one is the duplex system that has a physical redundancy, and the other one is the checkpointing scheme that rolls back to the last checkpoint at a failure. The average execution time and availability are important factors for measuring the performance of the fault-tolerant systems. However, in fault-tolerant real-time systems with a time constraint, meeting the time constrain instead of reducing the average execution time is the most important factor in the performance evaluation. We analyze and compare the performance of two fault-tolerant scheme (the duplex system and the checkpointing scheme) for real-time applications.