Browse > Article

Effect of Ailanthus altissima Water Extract on Cell Cycle Control Genes in Jurkat T Lymphocytes  

전병훈 (원광대학교 한의과대학 병리학교실)
황상구 (원광대학교 한의과대학 병리학교실)
이형철 (원광대학교 한의과대학 병리학교실)
김춘관 (원광대학교 한의과대학 병리학교실)
김대근 (원광대학교 한의과대학 병리학교실)
이기옥 (원광대학교 한의과대학 방제학교실)
윤용갑 (원광대학교 한의과대학 방제학교실)
Publication Information
YAKHAK HOEJI / v.46, no.1, 2002 , pp. 18-23 More about this Journal
Abstract
Ailanthus altissima has been used to settle an upset stomach, to alleviate a fever and as an insecticide. We reported that the water extract of A. altissima induced apoptotic cell death in Jurkat T-acute Iymphoblastic leukemia cells. Here, we showed the dose-dependent inhibitions of cell viability by the extract, as measured by cell morphology. The cell cycle control genes are considered to play important roles in tumorigenesis. The purpose of the present study is also to investigate the effect of A. altissima on cell cycle progression and its molecular mechanism in the cells. The level of p21 protein was increased after treatment of the extract, whereas both Bcl-2 and Bax protein levels were not changed. These results suggest that A. altissima induces apoptotic cell death via p21-dependent signaling pathway in Jurkat cells which delete wild type p53. Gl checkpoint related gene products tested (cyclin D3, cyclin dependent kinase 4, retinoblastoma, E2Fl) were decreased in their protein levels in a dose-dependent manner after treatment of the extract Taken together, these results indicate that the increase of apoptotic cell death by A. altissima may be due to the inhibition of cell cycle in Jurkat cells.
Keywords
Ailanthus altissima; water extract; apoptosis; cell cycle; G1 checkpoint; Jurkat T-ALL cells;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that acclerates programmed cell death /
[ Oltvai, Z. N.;Milliman, C. L;Korsmeyer, S. J. ] / Cell   DOI
2 The retinoblastoma tumor suppressor protein /
[ Wang, J. Y.;Knudsen, E. S.;Welch, P. J. ] / Adv. Cancer Res.   DOI
3 /
[ 保健社會部 ] / 大韓藥典外韓藥(生藥)規格集
4 Cancer cell cycles /
[ Sherr, C. J. ] / Science   DOI
5 Retinoblastoma protein represses transcription by recruiting a histone deacetylase /
[ Magnaghi-Jaulin, L.;Groisman, R.;Naguibneva, I.;Robin, P.;Lorain, S.;Le Villain, J. P.;Troalen. F.;Trouche. D.;Harel-Bellan. A. ] / Nature   DOI   ScienceOn
6 How cells cycle toward cancer /
[ Marx, J. ] / Science   DOI
7 Cell cycle control in mammalian cells: role of cyclins, cyclin depedent kinases(CDKs), growth suppressor genes and cyclin-dependent kinase inhibitors(CKIs) /
[ Grana, X.;Reddy, E. P. ] / Oncogene
8 /
[ 방약중 ] / 실용중의내과학
9 저근백피 성분의 생리활성에 관한 연구(I) : 메탄올 추출물과 클로로포룸 분획이 Epoxide 분해계에 미치는 영향 /
[ 김종;최종원;김혜경;박수원;이창규 ] / 생약학회지
10 Oncogene : 20 years later /
[ Verma, I. M.;Vogt, P. K. ] / Genes Dev.   DOI   ScienceOn
11 Braking the cycle /
[ Hunter, T. ] / Cell   DOI   ScienceOn
12 /
[ Tang, W.;Eisenbrand, G. ] / Chinese drugs of plant origin
13 The p53 network /
[ Agarwal, M. L.;Taylor, W. R.;Chernov, M. V.;Chernova, O. B.;Stark, G. R. ] / J. Biol. Chem.   DOI   ScienceOn
14 Current status of the NCI plant and animal product program /
[ Suffness, M.;Douros, J. ] / J. Nat. Prod.   DOI
15 A new marker for human cancer cells /
[ Ashall, F.;Bramwell, M. E.;Harris, H. ] / Lancet
16 A new regulatory motif in cell-cycle control causing specific inhibition of cyclin D/CDK4 /
[ Serrano, M.;Hannon, G. J.;Beach, D. ] / Nature   DOI
17 /
[ 白洪龍 ] / 辨證施治槪要
18 Exit from G1 and S phase of the cell cycle is regulated by repressor complexes containing HDAC-Bb-hSWI/SNF and Rb-hSWI/SNF /
[ Zhang, H. S.;Gavin, M.;Dahiya, A.;Postigo, A. A.;Ma, D.;Luo, R. X.;Harbour, J. W. ] / Cell   DOI   ScienceOn
19 Human cyclin E, a nuclear protein essential for the G1-to-S phase transition /
[ Ohtsubo, M.;Theodoras, A. M.;Schumacher, J.;Robert, J. M.;Pagano, M. ] / Mol. Cell Biol.   DOI
20 Mammalian G1 cyclins and cell cycle progression /
[ Sherr, C. J. ] / Proc. Assoc. Am. Physicians
21 /
[ 江蘇新醫院 ] / 中葯大?典(제1판)
22 저근백피 Tar의 항균작용에 관한 연구 /
[ 주화균;노영수;김신규 ] / 경희약대논문집