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Effect of the Water Extract of Albizzia julibrissin on Cell Cycle Progression in the Human Leukemic Jurkat Cells  

Hwang, Sang-Gu (Department of Pathology, College of Oriental Medicine, Wonkwang University)
Lee, Hyung-Chul (Department of Pathology, College of Oriental Medicine, Wonkwang University)
Kim, Dae-Geun (Department of Pathology, College of Oriental Medicine, Wonkwang University)
An, Won-Gun (Department of Biology, College of Natural Sciences, Kyungpook University)
Jeon, Byung-Hun (Department of Biology, College of Natural Sciences, Kyungpook University)
Publication Information
Korean Journal of Pharmacognosy / v.33, no.1, 2002 , pp. 29-34 More about this Journal
Abstract
Albizzia julibrissin belonging to the family Leguminosae has been used for the treatment of contusion, sore throat, amnesia, and insomnia in Oriental traditional medicine. The water extract of A. julibrissin induced apoptosis in Jurkat T-acute lymphoblastic leukemia (ALL) cells as measured by cell morphology. The capability of this herb medicine to induce apoptosis was associated with proteolytic cleavage of specific target protein such as beta-catenin protein suggesting the possible involvement of caspases. The purpose of the present study is also to investigate the effect of A. julibrissin on cell cycle progression. Our results showed that GI checkpoint related gene products (cyclin D1, cyclin dependent kinase 4, retinoblastoma, E2F1) were decreased in their protein levels in a dose-dependent manners after treatment of the extract. These results indicate that the increase of apoptotic cell death by A. julibrissin may be due to the inhibition of cell cycle progression in wild type p53-lacking Jurkat cells.
Keywords
Albizzia julibrissin; Water extract; Acute lymphoblastic leukemia; Jurkat T cells; Cell cycle; Gl checkpoint;
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1 김동희, 김성훈(1999) 가미지황탕, 가미사군자탕 및 가미군자지창탕의 항종양활성. 대전대학교 한의학연구소 논문집 8: 131-146
2 Ohtsubo, M., and Roberts, J. M. (1993) Cyclin-dependentregulation of Gl in mammalian fibroblasts. Science 259:1908-1912   DOI   PUBMED
3 Sherr, C. J. (1994) The ins and outs of Rb: coupling geneexpression to the cell cycle clock. Trends Cell Biol. 4: 15-18   DOI   PUBMED   ScienceOn
4 Hunter, T., and Pines, J. (1994) Cyclins and cancer II: cyclinD and CDK inhibitors come of age. Cell 79: 573-582   DOI   ScienceOn
5 황상구, 이형철, 김춘관, 김대근, 송호준, 박영준, 김원신,전병훈(2001) p53-결핍 Jurkat T 임파구의 세포사멸에 대한 계혈등 물 추출물의 세포주기 억제효과. 동의생리병리학회지 15: 887-892
6 Vaux, D. L. (1993) Toward an understanding of themolecular mechanisms of physiological cell death. Proc.Natl. Acad. Sci. USA 90: 786-789   DOI   ScienceOn
7 Marx, J. (1994) How cells cycle toward cancer. Science 263:319-321   DOI   PUBMED
8 Chen, S. P., and Zhang, R. Y. (1997) Studies on the triterpenesapogenine from Albizziae Cortex. Acta Pharm. Sin. 32: 144-147
9 Hwang, S.-G., Park, J. U., Lee, H. C., Joo, W. H., Cho, Y.-K., Moon, J.-Y. (2000) Proteolysis of beta-catenin inapoptotic Jurkat cells. J. Life Sci. 10: 57-63
10 Ruddon, R. W.(1990) Chemical carcinogenesis. In prin-ciples of drug action (3rd.), Churchill Livingstone,p.735
11 황상구, 이형철, 김춘관, 김용익, 주성민, 김윈신, 전병훈(2001) 백혈병세포주 Jurkat T 세포에서 합환피(Albizziajulibrissin) 물 추출물의 아포토시스 유도 효과. 약학회지45: 730-738
12 Nevins, J. R. (1992) E2F: a link between the Rb tumor suppressor protein and viral oncoproteins. Science 258: 424-429   DOI   PUBMED
13 최승훈(1995) 東醫腫痬學. 杏林出版社, pp. 32-34, 서울
14 이봉우(1994) 防毒湯의 항종양효과와 면역반응에 관한 실험적 연구. 대한한의학회지 15: 245-263
15 문구, 정병학, 김병주(1999) 암 동서의 결합치료. 원광대학교출판국, pp. 745-767, 익산
16 Chen, S. P., Zhang, R. Y., Ma, L. B., and Tu, G. Z. (1997)Structure determination of three saponins from the stem barkof Albizzia julibrissin Durazz. Acta Pharm. Sin. 32: 110-115
17 Kaneta, M., Hikichi, H., Endo, S., and Sugiyama, N. (1980)Identification of flavones in sixteen Leguminosae species.Aeric. Biol. Chem. 44: 1407-1512   DOI
18 Chamsuksai, P., Choi, J. S., and Woo, W. S. (1981) 3',4',7-trihydroxyflavone in Albizzia julibrissin. Arch. Pharm. Res.4: 129-133   DOI
19 Woo, W. S., and Kang, S. S. (1984) Isolation of a newmonoterpene conjugated triterpenoid from the stem bark of Albizzia julibrissin. J. Nat. Prod. 47: 547-549   DOI   PUBMED
20 Pardee, A. B. (1989) Gl events and regulation of cellproliferation, Science 246: 603-608   DOI   PUBMED
21 대한병리학회(1990) 病理學. 高文社, p. 225, 서울
22 전국분자생물학교수(2000) 세포학. 한우리, pp599-636,서울
23 Weinberg, R. A. (1995) The retinoblastoma protein and cellcycle control. Cell 81: 323-330   DOI   PUBMED   ScienceOn
24 Wyllie, A. H. (1992) Apoptosis and the reguladon of cellnumbers in normal and neoplastic tissues: an overview.Cancer Meta. Rev. 11: 95-103   DOI   ScienceOn
25 대한의학협회 분과학회(1992) 암의 진단과 치료. 여문각,서울
26 Tang, W., and Eisenbrand, G. (1992) Chinese drugs of plantorigin. Springer-Verlag, New York
27 Kinjo, J., Fukui, K., Higuchi, H., and Nohara, T. (1991) The first isolation of lignan tri- and tetra-glycosides. Chem.Pharm. Bull. 39: 1623-1625   DOI