• Electrical & Electronic Materials
• /
• v.23 no.3
• /
• pp.31-39
• /
• 2010

• Electrical & Electronic Materials
• /
• v.20 no.2
• /
• pp.3-11
• /
• 2007

• Korean Journal of Microbiology
• /
• v.32 no.1
• /
• pp.40-46
• /
• 1994

The pcb genes of Pseudomonas sp. DJ-12 coded for the catabolism of polychlorinated biphenyl (PCBs) and biphenyl. The products of the pcbCD genes were 2,3-dihydroxy-4'-chlorobiphenyl dioxygenase and meta-cleavage product (MCP) hydrolase, which acted on degradation of 2,3-dihydroxy-4'-chlorobiphenyl to 4-chlorobenzoate. The pcbCD genes were cloned in E. coli XLl-Blue, and then the pcbD gene was further subcloned. As a metabolite transformed from 2,3-dihydroxybiphenyl by the cloned cell of E coli CU103, benzoate was detected by the resting cell assay. The enzyme activities of 2,3-dihydroxybiphenyl dioxygease and MCP hydrolase produced in the cloned cells E. coli CU103 and CU105 were about 17 and 3 times higher than those of Pseudomonas sp. DJ-12, respectively.

• BMB Reports
• /
• v.28 no.1
• /
• pp.51-56
• /
• 1995

Thioredoxin is a small redox protein with an active-site disulfide/dithiol, and is ubiquitous in bacteria, plants, and animals. To investigate the structure-function relationship of thioredoxin, the genes encoding Escherichia coli thioredoxin and Corynebacterium nephridii thioredoxin C3 were fused via a common restriction site in the nucleotide sequence coding for the active site of the proteins to generate two chimeric thioredoxins, designated E-C3(N to C-terminal) and C3-E. The hybrid thioredoxin genes were put under the T7 promoter and their productions were confirmed. The two hybrid thioredoxins complemented phenotypes of a thioredoxin-deficient E. coli strain. A strain containing the C3-E hybrid thioredoxin supported growth of the T7 phage, whereas a strain expressing the E-C3 hybrid thioredoxin did not. However, both hybrids supported growth of M13 phages. The two hybrid thioredoxins were also characterized in other aspects. Differences in activity between the hybrid thioredoxins were attributed to altered interactions of the N- and C-terminal domains of the molecule, which produced changes in the three-dimensional structure of the active site region.

• Mycobiology
• /
• v.37 no.3
• /
• pp.225-229
• /
• 2009

Single spore isolates of Plasmodiophora brassicae e4 and e9 obtained from diseased Chinese cabbage were identified as race 4 and race 9, respectively, by the Williams' differential variety set. To confirm the possibility of variation in same generation and progeny of a single spore isolate of P. brassicae, random amplified polymorphic DNA (RAPD) analysis was conducted using the URP 3, 6 and OPA 7 primers. There was no difference in band type at each part of the gall of Chinese cabbage obtained by inoculation of e4 and e9 and amplification using the URP 3 and 6 primers when the same generation was analyzed. In addition, the progeny analysis, which was expanded to the third generation and conducted using the URP 3 and OPA 7 primers, revealed no differences in the band type of the e4 isolate. Based on these results, the single spore isolate of P. brassicae was genetically stable.

• Journal of Family Resource Management and Policy Review
• /
• v.10 no.3
• /
• pp.149-169
• /
• 2006

As e-commerce is expanding rapidly, the consumer's damage in the e-commerce is also dramatically increased. This research was mainly focused on the consumer's damage experiences through the e-commerce, the consumer's behavior's when they were confronted with troubles, and the way to handle their damages in the e-commerce. This research was made with the intention of not only supplying the academic data on the consumer's damage but also understanding the consumer's basic behavior patterns in e-commerce. This research was supported by the surveys conducted to consumers who had previously experienced fashion e-commerce. Total 1230 questionnaires out of returned questionnaires were sampled for the final analysis. Main findings are as follows. 1) More than 90% of respondents answered that they had experienced troubles 2) Most consumers did complain their damages actively and tried to obtain proper cure from the seller.

• Journal of the Korean Society for Aviation and Aeronautics
• /
• v.14 no.3
• /
• pp.40-50
• /
• 2006

This study investigates how airline customers evaluate e-ticket process newly adopted by many of airlines. Customers are highly satisfied with the easy of confirmation on their itineraries and the channel provided through while they are worry about the likelihood of leakage of personal information during e-ticket process. Highly experienced customers on the usage of e-ticket are more concerned about ancillary aspects such as visual image of I.T.R(Itinerary and Receipt) and travel information than functional aspect such as easy of confirmation on itinerary. And also experienced customers are more likely to repurchase e-ticket and when purchasing tickets they do it through internet and call center of airlines rather than travel agencies and ticket office of airlines.

• Asia pacific journal of information systems
• /
• v.15 no.3
• /
• pp.111-132
• /
• 2005

Company-wide initiatives mandate understanding the benefits of adopting e-Business. A benefits evaluation may ensure that the company's Web site in integral, rather than supplemental, to its business and revenue goals. Our research is motivated by the goal of extracting anticipated benefits factors of e-Business in Small and Medium-sized Enterprises(SMEs) in Korea. And we examined the impacts of anticipated benefits factors on the extent of assimilation in SMEs. Eight benefits factors emerged from this study: productivity, competitiveness, management, customer relationship, information quality, interorganization, cost down, and marketing. And competitiveness, customer relationship, interorganization, and marketing were influenced assimilation of e-Business in SMEs. But productivity, management, information quality, cost down did not.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.7
• /
• pp.969-978
• /
• 2014

The aprE2 gene with its prosequence from Bacillus subtilis CH3-5 was overexpressed in Escherichia coli BL21(DE3) by using plasmid pET26b(+). After IPTG induction, active and mature AprE2 was produced when cells were grown at $20^{\circ}C$, whereas inactive and insoluble enzyme was produced in a large amount when cells were grown at $37^{\circ}C$. The insoluble fraction was resuspended with 6 M guanidine-HCl and dialyzed against 2 M Tris-HCl (pH 7.0) or 0.5 M sodium acetate (pH 7.0) buffer. Then active AprE2 was regenerated and purified by a Ni-NTA column. Purified AprE2 from the soluble fraction had a specific activity of $1,069.4{\pm}42.4U/mg$ protein, higher than that from the renatured insoluble fraction. However, more active AprE2 was obtained by renaturation of the insoluble fraction. AprE2 was most stable at pH 7 and $40^{\circ}C$, respectively. The fibrinolytic activity of AprE2 was inhibited by PMSF, but not by EDTA and metal ions. AprE2 degraded $A{\alpha}$ and $B{\beta}$ chains of fibrinogen quickly, but not the ${\gamma}$-chain. AprE2 exhibited the highest specificity for N-succinyl-Ala-Ala-Pro-Phe-pNA. The $K_m$ and $k_{cat}/K_m$ of AprE2 was 0.56 mM and $3.10{\times}10^4S^{-1}M^{-1}$, respectively.

• Journal of Life Science
• /
• v.26 no.2
• /
• pp.241-246
• /
• 2016

In the present study, ethanol extracts and their subsequent organic solvent fractions were extracted from the lees of Ehwa Makgeolli containing oriental herbs, a commercialized traditional Korean rice wine, and the prepared lees samples were designated as from KSD-E3-1 to KSD-E3-5. First, their effects on cell viability and on the expression of pro-apoptotic ATF3 and NAG-1 genes in human colorectal HCT116 cells were investigated. Among the treated lees samples, the hexane fraction (KSD-E3-2) and the ethyl acetate fraction (KSD-E3-3) of lees extracts from Ehwa Makgeolli significantly reduced cell viabilities, in a dose dependent manner. The treatment with KSD-E3-2 and KSD-E3-3 also increased the expression of pro-apoptotic NAG-1 and ATF-3 genes and their proteins, which were detected with RT-PCR and Western blot analysis, respectively. In addition, poly-(ADP-ribose) polymerase (PARP) cleavage was detected by treatment with the fraction KSD-E3-3, indicating that KSD-E3-3 could induce apoptosis in HCT116 cells. Interestingly, this PARP cleavage was recovered by transfection of NAG-1 small interfering RNA. The results indicate that NAG-1 is one of the genes responsible for apoptosis induced by the fraction KSD-E3-3 from Ehwa Makgeolli. Overall, the findings may help in understanding the molecular mechanisms of the anti-proliferative and pro-apoptotic activities mediated by the lees of Ehwa Makgeolli.