Browse > Article
http://dx.doi.org/10.4489/MYCO.2009.37.3.225

Chinese Cabbage Club root Pathogen, Plasmodiophora brassicae, Is Genetically Stable  

Heo, Seung-Hwan (National Plant Quarantine Service)
Jang, Se-Jeong (Seocheon Agricultural Technology Center)
Choi, Jin-Soo (Department of Agricultural Biology, Chungnam National University)
Jang, Chang-Soon (Department of Agricultural Biology, Chungnam National University)
Song, Jeong-Young (Department of Agricultural Biology, Chungnam National University)
Kim, Hong-Gi (Department of Agricultural Biology, Chungnam National University)
Publication Information
Mycobiology / v.37, no.3, 2009 , pp. 225-229 More about this Journal
Abstract
Single spore isolates of Plasmodiophora brassicae e4 and e9 obtained from diseased Chinese cabbage were identified as race 4 and race 9, respectively, by the Williams' differential variety set. To confirm the possibility of variation in same generation and progeny of a single spore isolate of P. brassicae, random amplified polymorphic DNA (RAPD) analysis was conducted using the URP 3, 6 and OPA 7 primers. There was no difference in band type at each part of the gall of Chinese cabbage obtained by inoculation of e4 and e9 and amplification using the URP 3 and 6 primers when the same generation was analyzed. In addition, the progeny analysis, which was expanded to the third generation and conducted using the URP 3 and OPA 7 primers, revealed no differences in the band type of the e4 isolate. Based on these results, the single spore isolate of P. brassicae was genetically stable.
Keywords
Genetic variation; Plasmodiophora brassicae; Progeny analysis; RAPD; Single spore isolate;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Buczacki, S. T, Toxopeus, H., Mattush, R, Johnston, T. D., Dixon, G. R. and Hobolth, L. A. 1975. Studies of physiologic specialization in PIusmodiophora brassicae: proposals for attempted rationalization through an international approach. Trans. Br. Mycol. Soc. 65:295-303   DOI
2 Graf, F., Fahling, M. and Siemens, J. 2004. Chromosome poIymorphism of the obligate biotrophic parasite Plasmodiophora hrassicue. J. Phytopathology 152:86-91   DOI   ScienceOn
3 Jang, C. S. 2005. Development of diagnostic system of root rot disease of ginseng caused by Cylindrocarpon destructans using nested PCR from soil. Ms. D. Thesis. Chungnam National University
4 Fahling, M., Graf, H. and Siemens, J. 2004. Characterization of single-spore isolate population of Plusmodiophora brassicae resulting from a single club. J. Phytopathology 152:438-444   DOI   ScienceOn
5 Kageyama, K., Kamimura, Y. and Hyakumachi, M. 1995. A Simple inoculation method with a single resting spore of Plasmodiophora brassicae. Ann. Phytopathol. Soc. Jpn. 61:415-418
6 Buczacki, S. T. 1977. Root infections from single resting spores of Plasmodiophora brassicae. Trans. Br. Mycol. Soc. 69:328-329   DOI
7 Some, A., Manzanares, M. J., Laurens, F., Baron, F., Thomas, G and Rouxel, F. 1996. Variation for virulence on Brassica napus L. amoongst Plasmodiophora brassicae collections from France and derived single-spore isolates. Plant Pathol. 45:432-439   DOI   ScienceOn
8 Moller, M. and Harling, R. 1996. Randomly amplified poIymorphic DNA(RAPD) profiling of Plasmodiophora brassicae. Lett. Appl. Microbiol. 22:70-75   DOI
9 Castlebyry, L. A., Maddox, J. V. and Glawe, D. A. 1994. A technique for the extraction and purification of viable Plasmodiophora brassicae resting spores from host root tissue. Mycologia 86:458-460   DOI
10 Doyle, J. J. and Doyle, J. L. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13
11 Heo, S. H. 2004. Characteristics analysis of genetic variation in Plasmodiophora brassicae from Korea. Ms. D. Thesis. Chungnam National University
12 Voorrips, R. E. 1995. Plasmodiophora brassicae: aspects of pathogenesis and resistance in Brassica oleracea. Euphytica 83:139-146   DOI   ScienceOn
13 Jang, S. J. 2006. Characteristics of infection and novel singlespore isolation method through two-step inoculation of clubroot pathogen. Ptasmodiophora brassicae. Ms. D. Thesis. Chungnam National University
14 Klewer, A., Luerben, H., Graf, H. and Siemens, J. 2001. Restriction fragment length polymorphism markers to characterize PIasmodiophora brassicae single spore isolates with different virulence patterns. J. Phytopathology. 149:121-127   DOI   ScienceOn
15 Tinggal, S. H. and Webster, J. 1981. Technique for single spore infection by Plasmodiophora brassicae. Trans. Br. Mycol. Soc. 76:187-190   DOI
16 Williams, P. H. 1966. A system tor the determination of race of Plasmodiophora brassicae that infect cabbage and rutabaga. Phytopathology 56:624-626