Mycobiology

The Korean Society of Mycology (한국균학회)
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Chinese Cabbage Club root Pathogen, Plasmodiophora brassicae, Is Genetically Stable

  • Heo, Seung-Hwan (National Plant Quarantine Service) ;
  • Jang, Se-Jeong (Seocheon Agricultural Technology Center) ;
  • Choi, Jin-Soo (Department of Agricultural Biology, Chungnam National University) ;
  • Jang, Chang-Soon (Department of Agricultural Biology, Chungnam National University) ;
  • Song, Jeong-Young (Department of Agricultural Biology, Chungnam National University) ;
  • Kim, Hong-Gi (Department of Agricultural Biology, Chungnam National University)
  • Published : 2009.09.30
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Abstract

Single spore isolates of Plasmodiophora brassicae e4 and e9 obtained from diseased Chinese cabbage were identified as race 4 and race 9, respectively, by the Williams' differential variety set. To confirm the possibility of variation in same generation and progeny of a single spore isolate of P. brassicae, random amplified polymorphic DNA (RAPD) analysis was conducted using the URP 3, 6 and OPA 7 primers. There was no difference in band type at each part of the gall of Chinese cabbage obtained by inoculation of e4 and e9 and amplification using the URP 3 and 6 primers when the same generation was analyzed. In addition, the progeny analysis, which was expanded to the third generation and conducted using the URP 3 and OPA 7 primers, revealed no differences in the band type of the e4 isolate. Based on these results, the single spore isolate of P. brassicae was genetically stable.

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References

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