• 한국HCI학회:학술대회논문집
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• 한국HCI학회 2007년도 학술대회 2부
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• pp.582-591
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• 2007

지금까지 HCI분야의 많은 연구들은 사용성 (Usability)을 중요한 연구 주제로 인식하여 왔다. 그러나 연구의 범위를 사용성에 한정하는 것은 HCI 분야의 잠재성을 좁히고, 사용자 경험 (User Experience)의 개념도 좁은 범위로 한정시키게 된다. 따라서 향후 HCI 분야의 연구는 정보 기술의 사용성 측면의 뿐만 아니라, 정보 기술의 사용을 통한 다양한 사용자 경험을 구조적인 관점에서 살펴보아야 한다. 즉 사용성 중심의 연구가 아닌, 보다 전반적이고 구조적인 측면에서 사용자 경험을 살펴보는 새로운 방향의 HCI 연구가 필요할 것으로 보인다. 이와 같이 사용자 경험을 보다 전반적이고 구조적인 측정에서 이해하기 위해서는 사용자가 특정 정보 기술의 사용을 통해 추구하는 가치 (Value)와 그와 같은 가치를 실현 시킬 수 있는 정보 기술의 속성 (Attribute) 및 결과 (Consequence), 그리고 속성, 결과, 가치 간 연관 관계를 구조적인 측면에서 살펴보아야 한다. 본 연구는 사용자 가치에 대한 대표적인 이론인 Means-End Chain Theory와 속성, 결과, 가치 간 연관 관계를 파악하기 위한 정성적 연구 방법론인 Laddering 기법을 기반으로 사용자 경험 구조 파악 및 가치 중심적 HCI (Value-Centered HCI)를 위한 새로운 연구 방법론을 제안하고자 한다. 또한 본 연구에서는 새롭게 제안된 방법론을 통해 모바일 인터넷 서비스에 대한 사용자 경험 구조를 도출하고, 모바일 인터넷 상황에서 도출된 결과 및 본 연구에서 새롭게 제안된 방법론의 이론적이고 실용적인 의의를 제시하고자 한다.

• 대한의생명과학회지
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• 제4권2호
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• pp.129-135
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• 1998

Cytochrome P45O 2El (CYP2El)의 retropseudogene이 사람에 존재하는지 확인하기 위해 혈액에서 분리한 DNA를 주형으로 한 polymerase chain reaction (PCR)을 수행하였다. Primer는 CYP2E1 유전자를 기초하여 여러개 제작하되 적절한 조합에 따라 정상유전자와 retropseudogene이 동시에 증폭될 수 있도록 고안하였다. 본 실험의 결과 그동안 예상은 되었지만 DNA차원에서 미처확인되지 못하였던 CYP2E1의 retropseudogene을 직접 증폭해낼 수 있었다. 세부 구조는 Southern blotting과 DNA 염기서열 결정에서 최종 분석되었다. PCR 반응으로 증폭된 부분에서는 염기서열이 mRNA와 완전히 일치하고 있는 점으로 미루어서 CYP2E1의 retropseudogene은 비교적 최근에 발생했을 것으로 추정되었다.

• 한국작물학회지
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• 제63권3호
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• pp.210-218
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• 2018

This study was conducted to examine the influence of different transplanting dates on rice quality and starch properties (morphology and pasting properties) of rice varieties that may be used for the production of rice flour. Three rice flour varieties, 'Seolgaeng', 'Hangaru', and 'Milyang317', were transplanted on May 20, May 30, June 10, and June 20. The peak viscosity decreased with a delay in the transplanting date. However, the amylose content increased with a delay in the transplanting date, whereas that of protein decreased. Amylopectin short-branch chain content increased in the rice varieties that were transplanted on May 30. The morphology of the starch granule of the varieties was determined by SEM. No apparent external difference in the starch granules was observed for the different transplanting dates. These results indicate that the transplanting date influenced the amylopectin structure and pasting properties, which led to changes in the physicochemical characteristics of rice starch.

• Journal of Microbiology and Biotechnology
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• 제13권2호
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• pp.182-190
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• 2003

A new phaC gene cluster encoding polyhydroxyalkanoate (PHA) synthase I PHA depolymerase, and PHA synthase II was cloned using the touchdown PCR method, from medium-chain length (mcl-) PHA-producing strain Pseudomonas putida KT2440. The molecular structure of the cloned phaCl gene was analyzed, and the phylogenic relationship was compared with other phaCl genes cloned from Pseudomonas species. The cloned phaCl gene was expressed in a recombinant E. coli to the similar level of PHA synthase in the parent strain P. putida KT2440, but no significant amount of mcl-PHA was accumulated. The isolated phaCl gene was re-introduced into the parent strain P. putida KT2440 to amplify the PHA synthase I activity, and the recombinant P. purida accumulated mcl-PHA more effectively, increasing from 26.6 to $43.5\%$. The monomer compositions of 3-hydroxylalkanoates in mcl-PHA were also modified significantly in the recombinant P. putida enforcing the cloned phaCl gene.

• Mass Spectrometry Letters
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• 제2권1호
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• pp.8-11
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• 2011

Two types of glycerolipids [monoacylglycerols (MAG) and cyclitols] were isolated by reversed phase high-performance liquid chromatography from the methanol extracts of a marine sponge, and analyzed by fast atom bombardment mass spectrometry (FAB-MS) in positive-ion mode. FAB mass spectra of these compounds yielded protonated molecules $[M + H]^+$ and abundant sodiated molecules $[M + Na]^+$ from a mixture of 3-nitrobenzyl alcohol and NaI. The structures of these compounds were elucidated by FAB-collisional-induced dissociation (CID)-tandem mass spectrometry. We carried out collision-indused dissociation (CID) of these lipids in B/E-linked scan mode. The CID B/E-linked scan of $[M + H]^+$ and $[M + Na]^+$ precursor ions resulted in the formation of numerous characteristic product ions through a series of dissociative processes. The product ions formed by charge-remote fragmentation (CRF) provided important information for the identification of the acyl chain structure substituted at the glycerol backbone. Some of the product the ions were diagnostic for the presence of a glycerol backbone or acyl chain structure.

• Journal of Microbiology and Biotechnology
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• 제20권10호
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• pp.1393-1396
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• 2010

The attachment of sugar to flavonoids enhances their solubility. Glycosylation is performed primarily by uridine diphosphate-dependent glycosyltransferases (UGTs). The UGT from Bacillus cereus, BcGT-1, transferred three glucose molecules into kaempferol. The structural analysis of BcGT-1 showed that its substrate binding site is wider than that of plant flavonoid monoglucosyltransferases. In order to create monoglucosyltransferase from BcGT-1, the error-prone polymerase chain reaction (PCR) was performed. We analyzed 150 clones. Among them, two mutants generated only kaempferol O-monoglucoside, albeit with reduced reactivity. Unexpectedly, the two mutants harbored mutations in the amino acids located outside of the active sites. Based on the modeled structure of BcGT-1, it was proposed that the local change in the secondary structure of BcGT-1 caused the alteration of triglucosyltransferase into monoglucosyltransferase.

• 대한안전경영과학회:학술대회논문집
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• 대한안전경영과학회 2011년도 추계학술대회
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• pp.499-506
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• 2011

새로운 IT 환경에서의 변화를 이해하고 경쟁력 있는 클라우드 생태계를 구축하며, 지속가능한 성장을 위한 경쟁정책을 수립하기 위해서는 먼저 우리나라 IT시장의 구조와 그 변화를 정확히 이해하는 것이 필요하다. IT시장의 구조를 이해하기 위한 과거의 선행연구들은 통신시장을 배경으로 수직적 가치사슬 구조를 중심으로 설명한 것이 대부분이었으나, 최근 들어 이슈로 부각하고 있는 클라우드 생태계에 관한 연구는 부족한 실정이다. 본 연구의 목적은 IT시장에서의 클라우드 서비스의 등장에 따른 가치사슬의 변화를 이해하는 것이다. 이를 위해 먼저 기존의 연구 문헌들을 통하여 기업 또는 산업의 공급 구조, 그리고 시장의 생태계를 설명하기 위해 사용하는 기본 틀인 가치사슬의 개념을 정의하고, 이를 통하여 우리나라 IT시장의 가치사슬에 대해, 특히 클라우드 서비스 도입이후의 우리나라 IT시장의 변화를 반영하여 그 구조의 변화를 설명하며, 이를 바탕으로 우리나라 IT시장을 진단하고 시장의 성장을 견인하는 요인들을 파악하도록 한다. 본 연구를 통해 가치사슬 관점에서 IT시장을 이해함으로써, 클라우드 서비스 산업의 경쟁력을 높이고, 향후 클라우드 기반으로 IT생태계를 구축하기 위한 이론적 근거로 활용되길 기대한다.

• 한국해양공학회지
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• 제31권4호
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• pp.299-307
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• 2017

The design of the mooring line to maintain the position of an offshore structure in rough marine environments is recognized as a very important consideration. Conventional fatigue evaluation of a mooring line was performed by considering the tensile force acting on the mooring line, but the mooring line broke after 238 days in the girassol area even though the expected fatigue life was expected to be longer. The causes of this event are known to be due to OPB/IPB (out-of-plane bending/in-plane bending) caused by chain link friction due to the excessive tensile strength of the mooring line. In this study, three models with different boundary conditions were proposed for fatigue analysis of a mooring line considering OPB/IPB. Interlink stiffness was calculated by nonlinear structure analysis and a stress concentration factor was derived. In addition, the sensitivity of interlink stiffness according to the magnitude of tensile force, large deformation effect, and coefficient of friction was analyzed, and the effect of critical elastic slip and bending moment calculation position on interlink stiffness was confirmed.

• 한국전기전자재료학회논문지
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• 제16권5호
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• pp.390-396
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• 2003

As the design rule of device continued to shrink, the contact resistance in small contact size became important. Although the conventional TiN/Ti structure as a ohmic layer has been widely used, we propose a new TiN/Co film structure. We characterized a contact resistance by using a chain pattern and a KELVIN pattern, and a leakage current determined by current-voltage measurements. Moreover, the microstructure of TiN/ Ti/ silicide/n$\^$+/ contact was investigated by a cross-sectional transmission electron microscope (TEM). The contact resistance by the Co ohmic layer showed the decrease of 26 % compared to that of a Ti ohmic layer in the chain resistance, and 50 ％ in KELYIN resistance, respectively. A Co ohmic layer shows enough ohmic behaviors comparable to the Ti ohmic layer, while higher leakage currents in wide area pattern than Ti ohmic layer. We confirmed that an uniform silicide thickness and a good interface roughness were able to be achieved in a CoSi$_2$ Process formed on a n$\^$+/ silicon junction from TEM images.

• Design & Manufacturing
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• 제11권3호
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• pp.35-40
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• 2017

PCR(Polymerase chain reaction) is a technique to replicate and amplify a desired part of DNA. It is used in various aspects such as DNA fingerprint analysis and rare DNA amplification of an extinct animal. Especially in the medical diagnosis field, it provides various measurement methods at the molecular level such as genetic diagnosis, and is a basic tool for molecular diagnostics. The internal shape of the plastic vial tube for PCR analysis used in the DNA detection process, and the surface roughness and internal cleanliness can affect detection and discrimination results. The plastic vial tube demanded by the developer of the PCR analysis equipment should be changed to a structure that eliminates the residual washing solution in the washing process to ensure the internal cleanliness. Thus the internal structure and the internal surface design for improving the PCR amplification efficiency are key issues to develop the plastic vial tube for the DNA detection process.