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Molecular Structure of PCR Cloned PHA Synthase Genes of Pseudomonas putida KT2440 and Its Utilization for Medium-Chain Length Polyhydroxyalkanoate Production  

Kim, Tae-Kwon (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University)
Shin, Hyun-Dong (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University)
Seo, Min-Cheol (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University)
Lee, Jin-Nam (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University)
Lee, Yong-Hyun (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University)
Publication Information
Journal of Microbiology and Biotechnology / v.13, no.2, 2003 , pp. 182-190 More about this Journal
Abstract
A new phaC gene cluster encoding polyhydroxyalkanoate (PHA) synthase I PHA depolymerase, and PHA synthase II was cloned using the touchdown PCR method, from medium-chain length (mcl-) PHA-producing strain Pseudomonas putida KT2440. The molecular structure of the cloned phaCl gene was analyzed, and the phylogenic relationship was compared with other phaCl genes cloned from Pseudomonas species. The cloned phaCl gene was expressed in a recombinant E. coli to the similar level of PHA synthase in the parent strain P. putida KT2440, but no significant amount of mcl-PHA was accumulated. The isolated phaCl gene was re-introduced into the parent strain P. putida KT2440 to amplify the PHA synthase I activity, and the recombinant P. purida accumulated mcl-PHA more effectively, increasing from 26.6 to $43.5\%$. The monomer compositions of 3-hydroxylalkanoates in mcl-PHA were also modified significantly in the recombinant P. putida enforcing the cloned phaCl gene.
Keywords
phaC gene cluster; phaCl; Pseudomonas putida KT2440; mcl-PHA; touchdown PCR; PHA accumulation;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
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1 Expression analysis of phbC coding for poly-3-hyoroxybutyrate (PHB) synthase of Rhodobacter sphaeroides /
[ Kho, D. H.;K. M. Yang;K. S. Kim;J. K. Lee ] / J. Microbiol. Biotechno.   과학기술학회마을
2 A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding /
[ Bradford, M. M. ] / Anal. Biochem.   DOI   ScienceOn
3 Synthesis of poly(3-hydroxyalkanoates) in Escherichia coli expressing the PHA synthase gene phaC2 from Pseudomonas aeruginosa: Comparison of PhaC1 and PhaC2 /
[ Qi, Q.;B. H. A. Rehm;A. Steinbuchel ] / FEMS Microbiol. Lett.   DOI   ScienceOn
4 Specificpurpose plasmid vectors II . Broad host tange, high copy number, RSF1010-derived vectors and a host0vector system for gene cloning in Pseudomonas /
[ Bagdasarian, M.;R. Lurz;B. Ruckert;F. C. H. Franklin;M. M. Bagdasarian;J. Frey;K. N. Timmis ] / Gene   DOI
5 A new metabolic link between fatty acid de novo synthesis and polyhydroxyalkanoic acid synthesis: The phaG gene from Pseudomonas putida KT2440 encodes a 3-hydroxyacyl-acyl carrer protein-coenzyme a transferase /
[ Rehm, B. H. A.;N. Kruger;A. Steinbuvhel ] / J. Biol. Chem.   DOI   ScienceOn
6 DNA sequencing with chain terminating inhibitor /
[ Sanger, F.;S. Nicklen;A. R. Coulson ] / Proc. Natl. Acad. Sci. USA   DOI   ScienceOn
7 Biosynthesis and characterization of poly(β-hydroxyalkanoates) produced by Pseudomonas oleovorans /
[ Gross, R. A.;C. DeMello;R. W. Lenz;H. Brandl;R. C. Fuller ] / Macromolecules   DOI   ScienceOn
8 Cloning and molecular analysis of poly(3-hydroxyalkanoate) biosynthesis genes in Pseudomonas aureofaciens /
[ Nishikawa, T.;K. Ogawa;R. Kohda;W. Zhixiong;H. Miyasaka;F. Umeda;I. Maeda;M. Kawase;K. Yagi ] / Curr. Microbiol.   DOI   ScienceOn
9 Manipulation of the gene gor poly-β-hydroxybutyric acid synthesis in Alcaligenes eutrophus /
[ Park, H. C.;J. S. Park;Y. H. Lee;T. L. Huh ] / Biotechnol. Lett.   DOI   ScienceOn
10 Production of poly-β-hydroxybutyrate by Alcaligenes eutrophus transformant barboring cloned genes /
[ Park, J. S.;T. L. Huh;Y. H. Lee ] / Biotechnol. Lett.   DOI   ScienceOn
11 Polyhydroxybutyrate synthase: Evidence for covalent catalysis /
[ Wodzinska, J.;K. D. Snell;A. Rhomberg;A. J. Sinskey;K. Biemann;J. Stubbe ] / J. Am. Chem. Soc.   DOI   ScienceOn
12 Biosynthesis and structural characteruzation of medium-chain-lenght poly(3-hydroxyalkanoates) produced by Pseudomonas aeruginosa from fatty acids /
[ Ballistreri, A.;M. Giuffriea, S. P. P. Guglielmino;S. Carnaza;A. Ferreri;G. Impallomeni ] / Int. J. Biol. Mactromol.   DOI   ScienceOn
13 Production of P(3-hydroxybutyrate-3-hydroxy-valerate) and P(3-hydroxybutyrate-4-hydroxybutyrate )using transformant Alcaligenes latus enforcing cloned owq phbC gene /
[ Seo, I. S.;Y. M. Jung;Y. H. Lee ] / J. Mircobiol Biotechnol.   과학기술학회마을
14 Physical characteristics of poly(3-hydroxyalkanoates) produced by Pseunomonas oleovorans grown in aliphatic hydrocarbons /
[ Preusting, H.;A. Nijnhus;B. Witholt ] / Macromolecules   DOI
15 Cloning, cnaracterization and comparison of the Pseudomonas meridocina polyhydroxyalkanoate synthases PhaC1 and PhaC2 /
[ Fein, S.;J. R. J. Paletta;A. Streinbuchel ] / Appl. Microbiol. Biotechnol.   DOI   ScienceOn
16 Polymerase-Chain-reaction-based detection of individual polyhydroxyalkanoate synthase phaC1and phaC2 genes /
[ Solaiman, D. K. Y. ] / Biotechnol. Lett.   DOI   ScienceOn
17 Cloning and molecular analysis of the poluy(3-hydroxyalkanoic acid) gene locus of Pseudomonas aeruginosa PAO1 /
[ Timm, A.;A. Steinbuchel ] / Eur. J. Biochem.   DOI   ScienceOn
18 Production of genetically engineered Pseudomonas /
[ Solaiman, D. K. Y.;R. D. Ashby;T. A. Foglia ] / Appl Microbiol. Biotechnol.   DOI   ScienceOn
19 Construction of transformant Alcaligenes latus enforcing cloned own phbC gene and characterization of poly-β-hydroxy-butyrate biosynthesis /
[ Lee, Y. H.;I. S. Seo;Y. M. Jung;T. L. Huh ] / Biotechnol. Lett.   DOI   ScienceOn
20 /
[ Sambrook, J.;E. F. Fritsch;T. Maniatis ] / Molecular Cloning: A Laboratory Manual (2nd ed.)
21 Comparison of phbC genes cloned from Ralstonia eutropha and Alcaligenes latus for utilization in metabolic engineering of polyhydroxylkanoate biosyntheis /
[ Shin, H. D. ;D. H. Oh;Y. M. Jung;S. Y. Kim;Y. H. Lee ] / Biotechnol. Lett.   DOI   ScienceOn
22 An improved method for solation of chromosomal DNA from vatious bacteria and cyanobacteria /
[ Mak, Y. M.;K. K. Ho ] / Nucleic Acids Res.   DOI   ScienceOn
23 Accumulation of a polyhydroxydecanoate from simple cartiohydrate substrates by Pseudomonas sp. strain /
[ Haywood, G. W.;A. J. Anderson;D. F. Ewing;E. A. Dawes ] / Appl. Environ. Microbiol.
24 Closedloop control of bacterial high-cell-density fed-batch cultures: Production of mcl-PHAs by Pseudomonas putida KT2442 under single-substrate and cofeeding coditions /
[ Kellerhals, M. B.;B. Kessler;B. Witholt ] / Biotechnol. Bioeng.   DOI   ScienceOn
25 Touchdown PCR to circumvent spurious priming during gene amplification /
[ Don, R. H.;P. T. Cox;B. J. Wainwtright;K. Baker;J. S. Mattick ] / Nucleic Acids Res.   DOI   ScienceOn
26 Metabolism on poly-β-hydroxybutyrate. I . Purification, composition, and properties of native poly-β-hydroxybutyrate granules from Bacillus megaterium /
[ Griebel, R.;Z. Smith;J. M. Merrick ] / Biochem.   DOI   ScienceOn
27 Rapid and specific identifiation of mediumchain-length polyhydroxyalkanoate synthase gene bypolyumerase chanin reaction /
[ Solaiman. D. K. Y.;R. D. Ashby;T. A. Foglia ] / Appl. Microbiol. Biotechnol.   DOI   ScienceOn
28 High frequency tranformation of Alcaligenes eutrophus producing poly-β-gydroxybutyric acid by electroporation /
[ Park, H. C.;K. J. Lim;J. S. Park;Y. H. Lee;T. L. Huh ] / Biotechnol. Tech.   DOI   ScienceOn
29 Cometabolism of ω-pjenylalkanoic acids with butyric acid for efficient production of aromatic polyesters in Pseudomonas putida BM01 /
[ Song, J. J. ;M. H. Choi;S. C. Yoon;N. E. Huh ] / J. Microbiol. Biotehnol.   과학기술학회마을
30 PCR cloning of type II polyhydroxyalkanoate biosynthesis genes from two PSEUDOMONAS STRAINS /
[ Zhang, G.;X. Hang;P. Green;K. P. Ho;G. Q. Chen ] / FEMS Microbiol. Lett.   DOI   ScienceOn
31 Polyhydroxyalkanoic acid /
[ Steinbuchel, A.;Byrom, D.(ed.) ] / Biomaterials: Novel Materials from Biological Sources
32 Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans: Identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of PHA /
[ Huisman, G. W.;E. Wonink;R. Meima;B. Kazemier;P. Terpstra;B. Witholt ] / J. Biol. Chem.
33 Enhanced biosynthesis of P(3HB-2HV) and P(3HB-4HB) by amplification of the cloned PHB biosynthesis genes in Alcaligenes eutrophus /
[ Lee, Y. H.;J. S. Park;T. L. Huh ] / Biotechnol. Lett.   DOI   ScienceOn
34 Biochemical and genetic analysis of PHA synthases and other proteins required for PHA synthesis /
[ Rehm, B. H. A.;A. Steinbuchel ] / Int. J. Biol. Macromol.   DOI
35 Production of polyhydroxyalkanoates by Pseudomonas nitroreducens /
[ Yao, J.;G. Zhang;Q. Wu;G. Q. Chen;R. Zhang ] / Antonie Van Leeuwenhoek   DOI   ScienceOn
36 Formation of polyesters by Pseudomonas oleovorans: Effect of substrates on formation and composition of poly-R-3-hydroxyalkanoates and poly-R-3-hydroxyalkenoates /
[ Lageveen, R. G.;G. W. Huisman;H. Preusting;P. Ketelaar;G. Eggink;B. Witholt ] / Appl. Environ. Microbiol
37 Ein Submersverfahren zur Kultur wasserstoffoxidierender Bakterien: Wachstumsphysiologische Untersuchungen /
[ Schlegel, H. G. ;H. Kaltwasser;G. Gottschalk ] / Arch. Mikrobiol.   DOI
38 PCR cloning of Pseudomonas resinovorans polyhydroxyalkanoate biosynthesis genes and expression in Escherichia coli /
[ Solaiman, D. K. Y.; ] / Biotechnol. Lett.   DOI   ScienceOn
39 Polyhydroxyalkanoate inclusion body-associated proteins and coding region in Bacillus megaterium /
[ McCool, G.J.;M. C. Cannon ] / J. Bacteriol.
40 Formation of polyesters consisting of medium-chain-length 3-hydroxyalkanoic acids from gluconate by Pseudomonas aeruginosa and other fluorescent Pseudomonads /
[ Timm, A.;A. Steinbuvhel ] / Appl. Environ. Microbiol.
41 A rapid gas chromatographic method for the determination of polyu-β-hydroxybutyric acid in microbial biomass /
[ Braunegg, G.;B. Sonneleitner;R. M. Lafferty ] / Eur. J. Appl. Microbiol.   DOI
42 Novel biodegradable aromatic plastics from a bacterial source. Genetic and biochemical studies of a route of the phenylacetyl -CoA catabolon /
[ Garcia, B.;E. R. Olivera;B. Minambres;M. Fernandez-Valverde;L. M. Canedo;M. A. Proeto;J. L. Garcia;M. Martinez;J. M. Luengo ] / J. Biol. Chem.   DOI
43 Cloning and molecular analysis of the poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyalkanoate) biosynthesis genes in Pseudomonas sp. strain 61-3 /
[ Matsusaki, H.;S. Manji;K. Taguchi;M. Kato;T. Fukui;Y. Doi ] / J. Bacteriol.