• Journal of the Korean Institute of Landscape Architecture
• /
• v.31 no.4
• /
• pp.82-89
• /
• 2003

This study was carried out to find the suitable root-ball size for the planting construction of landscape trees. Surveyed trees for this study were Cercidiphyllum japonicum and Prunus yedoensis, and the root-ball size was classified into 3D, 4D, 5D, and 6D(D meaning the diameter at the base of a tree). Visual ratings on a scale of 1 to 9 were used as a means for measuring the regrowth strength after planting tested trees. Test trees with 3, 4, 5 and 6D root-ball size were planted on March. 16, 2002 and visual ratings were measured up to April. 30, 2003. Cercidiphyllum japonicum and Prunus yedoensis started showing a difference in regrowth by each rootball size a month after planting. The regrowth quality of Cercidiphyllum japonicum was low at 3D, medium at 4∼5D and high at 6D root-ball size, while the regrowth quality of Prunus yedoensis was low at 3∼4D and medium-high at 5∼6D root-ball size. According to the above results, a root-ball size for good regrowth quality was a little bit different between Cercidiphyllum japonicum and Prunus yedoensis. 4D of Cercidiphyllum japonicum and 5D of Prunus yedoensis could be guidelines for root-ball diameters at the base of trees when planting. However, we concluded that 4D∼5D root-ball is the optimum guideline for regrowth when adapting this guideline to all landscape trees with 8∼l0cm diameter at the base.

• Natural Product Sciences
• /
• v.27 no.4
• /
• pp.280-283
• /
• 2021

Cercidiphyllum japonicum is being used for the treatment of obesity and liver fibrosis in Korean local clinics. In the present study, we tried to develop an analytical methodology for the determination of the chemical markers of Cercidiphyllum japonicum. Four chemicals, maltol (1), chlorogenic acid (2), quercetin (3), and avicularin (4), were selected for method validation, and the analytical conditions were optimized and validated using high-performance liquid chromatography coupled with an ultraviolet detector (HPLC-UV). Additionally, the seasonal variations of four markers were monitored every month for six months. The contents of four chemicals markers were most detected in a sample collected in June.

• Journal of the Korean Wood Science and Technology
• /
• v.44 no.4
• /
• pp.559-570
• /
• 2016

The EtOAc and $H_2O$ soluble fractions of Katsura tree (Cercidiphyllum japonicum Sieb. Et Zucc) bark extracts were chromatographed on a Sephadex LH-20 column with various aqueous MeOH. Gallic acid (1), methyl galate (2), kurigalin (3), 1,2,3,6-tetra-O-galloyl-${\beta}$-D-glucose (4) and 1,2,3,4,6-penta-O-galloyl-${\beta}$-D-glucose (5) were isolated from EtOAc fraction. Isocorilagin (6) and methyl galate (2) were separated from $H_2O$ fraction. The structure determination was done by $^1H$ and $^{13}C$ NMR. Of these isolated compounds, methyl galate (2), kurigalin (3) and isocorilagin (6) were isolated, for the first time, from the bark extracts of Cercidiphyllum japonicum.

• Journal of the Korean Wood Science and Technology
• /
• v.43 no.5
• /
• pp.558-565
• /
• 2015

Katsura tree (Cercidiphyllum japonicum Sieb. Et Zucc) leaves were collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, methylene chloride ($CH_2Cl_2$), ethylacetate (EtOAc), and $H_2O$. The EtOAc fraction was chromatographed on a Sephadex LH-20 column with various aqueous MeOH eluting solvents and finally treated with acetone-$H_2O$ (7:3, v/v) to isolate a gallotannin. According to the NMR analysis, including HSQC and HMBC, and with the comparison of authentic literature data, the isolate was elucidated as 6-m-digalloyl-1,2,3,4-tetra-O-galloyl ${\beta}$-D-(+)-glucose, one of hydrolyzable tannins and one of gallotannins. The compound was only gallotannin which was firstly isolated from the extracts of Katsura tree leaves, and has not been reported before in domestic tree sources.

• Journal of the Korean Wood Science and Technology
• /
• v.43 no.5
• /
• pp.591-599
• /
• 2015

Cercidiphyllum japonicum leaves were collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, methylene chloride ($CH_2Cl_2$), ethylacetate (EtOAc), and $H_2O$. A portion of EtOAc fraction (10 g) was chromatographed on a Sephadex LH-20 column, by the successively elution with various aqueous $MeOH-H_2O$ (1:9, fraction $1-2{\rightarrow}3:7$, fraction $3-5{\rightarrow}1:1$, fraction $6-9{\rightarrow}7:3$, fraction $10-13{\rightarrow}9:1$, fraction 14-16). Compound 2 was isolated from fraction 6 and compound 1 was separated from fraction 11 and 12. Compound 3 and 4 were purified from fraction 13. The isolated compounds were elucidated as quercetin-3-O-${\alpha}$-L-rhamnopyranoside (1), chlorogenic acid (2), quercetin-3-O-${\alpha}$-L-arabinofuranoside (3) and quercetin-3-O-${\beta}$-D-xylopyranoside (4) by the spectral and literature data, and by comparison with the authentic samples. These compounds were reported, for the first time, from the extracts of C. japonicum leaves. Also chlorogenic acid (2) has never been reported before in domestic tree species and can be used as an index compound for C. japonicum.

• Journal of the Korean Wood Science and Technology
• /
• v.44 no.4
• /
• pp.551-558
• /
• 2016

Katsura tree (Cercidiphyllum japonicum Sieb. Et Zucc) bark was collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, methylene chloride ($CH_2Cl_2$), ethylacetate (EtOAc), and $H_2O$. The $H_2O$ fraction was chromatographed on a Sephadex LH-20 column with various aqueous MeOH eluting solvents to isolate an ellagitannin. The isolate was elucidated as macabarterin, a polyoxygenated ellagitannin by NMR analysis, including HSQC, HMBC, Q-TOF MS, and with the comparison of authentic literature data. The compound was an ellagitannin which was isolated, for the first time, from the extracts of Katsura tree bark, and has never been reported before in domestic tree or plant sources.

• Journal of the Korean Wood Science and Technology
• /
• v.43 no.2
• /
• pp.238-247
• /
• 2015

Katsura tree (Cercidiphyllum japonicum Sieb. Et Zucc) leaves were collected, air-dried and extracted with 70% aqueous acetone, then concentrated and sequentially fractionated using n-hexane, $CH_2Cl_2$, EtOAc, and $H_2O$ to be freeze dried for antioxidant and ${\alpha}$-glucosidase inhibition activity tests. The antioxidant activity of the extracts was evaluated using DPPH (1,1-diphenyl 2-picrylhydrazyl) free radical scavenging assay. The test concentrations were adjusted to 500, 250, 125, 62.5, 31.25, 15.62, 7.81, 3.9, 1.95 and 0.97 ppm. The $H_2O$ and EtOAc fractions showed higher activities compared with the control, ${\alpha}$-tocopherol, at all concentrations. The crude fraction also gave better activity at the concentrations lower than 62.5 ppm. However, the nonpolar n-hexane and $CH_2Cl_2$ fractions gave prominently lower activities compared with the control at all concentrations. The $IC_{50}$ values of the crude, EtOAc, and $H_2O$ fractions exhibited 11.78, 4.29 and $9.80{\mu}g/m{\ell}$, respectively, compared with $12.08{\mu}g/m{\ell}$ of the control. But the n-hexane and $CH_2Cl_2$ fractions indicated 300 and $91.85{\mu}g/m{\ell}$ of $IC_{50}$, respectively. ${\alpha}$-Glucosidase inhibition activity was evaluated at the concentrations of 50, 25, 12.5, 6.3, 3.1, 1.6 and 0.8 ppm. The inhibition activities were increased according to as the increase of sample concentrations. However, the nonpolar n-hexane and $CH_2Cl_2$ fractions indicated very low inhibition activities compared with acarbose, a positive control. The EtOAc fraction showed very good capability as almost 100% compared with the control at the higher concentrations than 12.5 ppm and the crude fraction also indicated good potential as 95% and 100% at 25 and 50 ppm, respectively. The $H_2O$ fraction gave good inhibition value as 90% at 50 ppm although the value was lower than the control. These results showed that the polar fractions had better ${\alpha}$-glucosidase inhibition activities. The $IC_{50}$ values of the nonpolar fractions, n-hexane and $CH_2Cl_2$, showed very lower values as 468 and $103.6{\mu}g/m{\ell}$, respectively, than the control. ${\alpha}$-Glucosidase Inhibition Activity of the Extracts of Katsura Tree (Cercidiphyllum japonicum Sieb. Et Zucc) Leaves However, the polar fractions, crude, EtOAc and $H_2O$, showed 7.1, 3.7 and $13{\mu}g/m{\ell}$, respectively, indicating that these fractions can be used as natural bioresources for treating diabetes mellitus. Also ${\alpha}$-glucosidase inhibition activity had a positive correlation with antioxidant activity of the extracts.

• Journal of the Korean Wood Science and Technology
• /
• v.45 no.3
• /
• pp.250-257
• /
• 2017

Eight hydrolyzable tannin compounds, such as gallic acid (1), methyl gallate (2), kurigalin (3), 1,2,3,6-tetra-O-galloyl-${\beta}$-D-(+)-glucose (4), 1,2,3,4,6-penta-O-galloyl-${\beta}$-D-(+)-glucose (5), 6-m-digalloyl-1,2,3,4,6-penta-O-galloyl-${\beta}$-D-(+)-glucose (6), isocorilagin (7), macabarterin (8), were isolated from the EtOAc and $H_2O$ soluble fractions of Katsura tree (Cercidiphyllum japonicum) leaves, wood and bark. Then antioxidative and anti-inflammatory activity were evaluated on the each isolated compound. The antioxidative test was DPPH radical scavenging activity and all of the isolated compounds indicated much higher antioxidative values compare to the controls, BHT and ${\alpha}$-tocopherol. In the anti-inflammatory test measuring nitric oxide (NO) inhibition activity, methyl gallate, 1,2,3,6-tetra-O-galloyl-${\beta}$-D-glucose and 1,2,3,4,6-penta-O-galloyl-${\beta}$-D-glucose inhibited NO production, and especially, methyl gallate showed high inhibition activity. However, the anti-inflammatory activity of the hydrolyzable tannins did not show positive effect. Based on the above results, the hydrolyzable tannins of katsura tree may be used as one of the natural biomass sources that can substitute with the synthetic antioxidant.

• Journal of the Korean Institute of Landscape Architecture
• /
• v.18 no.2
• /
• pp.57-65
• /
• 1990

To select suitable siecies for barrier trees in the middle tmperature forest zone of Korea, sprout ratio, verdi cal density ratio and impenetrability were analyzed for 41 species including 9 evergreen conifer trees which had been transplanted 18 years ago and maintained as a hedge at Pocheon - Gun, Kyonggi -Do, Korea. 1. The species with high sprout ratio of hedges were Rosa multiflora, Lonicera maackii, Chamaecyparis pisifera, Thuja occidentalis in order at the upper portion, Thuja occidentalis, Rosa multiflora, Chamaecyparis pisifera, in order oa the middle portion, and Thuja occidentals, Chamaecyparis pisigera, Rosa multiflora, Cercdiphyllum japonicum, Cornus kousa, Ulmus pumila, Robinia pseudoacacia in order at the lower portion of hedges. 2. the species with high vertical density ratio were Thuja occidentalis, Chamaecyparis pisifera, Weigela subsessilis, Acer ginnala in order. 3.The species with hight impnetrability were Thuja occidentalis, Chamaecyparis pisifera, Forsythia Koreana, Juniperus rigida, Juniperus chinensis, Pinus strobus in order. 4. the values of correlation coefficents between the sprout ratio and impenetrability are given in table 3 and positive significant correlations can be observed in conifer trees.5. The species with high suitability for tree barrier were Thuja occidontalis, Chamaecyparis pisifera, Acer ginnala, Juniperus chinensis, Rosa multiflora, Counus kousa, Cercidiphyllum japonocum, Weigela subsessilis, Cornus officinalis, Philadelphus schrenckii in order.

• Korean Journal of Environment and Ecology
• /
• v.16 no.1
• /
• pp.10-21
• /
• 2002

This study was intended to investigate injury rates of landscape trees planted in the reclaimed seaside areas and to analyse their causes in planting environment. The planting environment such as soil properties, reclaimed depth of soil, soil hardness, salt consistency of soil, and drainage system were surveyed in 8 reclaimed seaside areas in the middle and southern regions of the country. Injury rates of 42 species, 1,233 trees and growth of branches in 6 species. 130 trees were measured and analysed to fond out salt-resistant trees in the reclaimed lands. The results of the survey are as follows; the average injury rate of evergreen trees was 32%. which was much lower than that of deciduous trees as 52%. The lower injured trees in the 8 reclaimed lands were Pinus thunbergii, Pinus parvinora. Juniperus chinensis 'Kaizuka', Pinus koraiensis, Acer trifdum, Koelreuteria paniculata and Metasequoia glyptostroboides. The higher injured trees were Acer palmatum, Magnolia kobus. Lagerstroemia indica, Diospiros kaki, Cercidiphyllum japonicum, Sorbus commixta, Prunus yedoensis, Pinus desinora, Chaenomeles sinensis, Albizzia julibrissin and Zelkowa serrata. At least the mounding of 1.7m depth of soil is needed from the tidal saline soil to the roots of trees to protect trees from salt injury Roots of trees were found to have infiltrated to 0.77m under the soil of which solidity was over 4.5kg/㎥. And 22 of total 25 soils were acid from pH 3.72 to pH 5.85. Injury rate of trees was much higher when they were detached from the sea, and planted on the drainage.