• Molecules and Cells
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• 제43권11호
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• pp.935-944
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• 2020

Aryl hydrocarbon receptor nuclear translocator (ARNT) plays an essential role in maintaining cellular homeostasis in response to environmental stress. Under conditions of hypoxia or xenobiotic exposure, ARNT regulates the subset of genes involved in adaptive responses, by forming heterodimers with hypoxia-inducible transcription factors (HIF1α and HIF2α) or aryl hydrocarbon receptor (AhR). Here, we have shown that ARNT interacts with DDB1 and CUL4-associated factor 15 (DCAF15), and the aryl sulfonamides, indisulam and E7820, induce its proteasomal degradation through Cullin-RING finger ligase 4 containing DCAF15 (CRL4^DCAF15) E3 ligase. Moreover, the two known neo-substrates of aryl sulfonamide, RNA-binding motif protein 39 (RBM39) and RNA-binding motif protein 23 (RBM23), are not required for ARNT degradation. In line with this finding, aryl sulfonamides inhibited the transcriptional activities of HIFs and AhR associated with ARNT. Our results collectively support novel regulatory roles of aryl sulfonamides in both hypoxic and xenobiotic responses.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2002년도 춘계학술대회 논문집
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• pp.890-893
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• 2002

This study is focused to predict the behavior of Al foam with closed-cell structure during the 3 point bending test and the upsetting test according to aspect ratio. We calculated characters of aluminum foams with closed-cell structure and took the simulation. The effects on the aspect ratio of the cell was investigated parametrically. The analysis was carried out on two models, First, the bending test in elasticity of the rectangular beam, and Second, the upsetting test in plasticity of the circular cylinder. In the analysis, the deformation of the beam and the cylinder was influenced by the aspect ratio of the cell. Further, We assumed that the geometry of feared aluminum cell change the stress and strain in the test.

• Molecular & Cellular Toxicology
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• 제4권2호
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• pp.170-176
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• 2008

The quantitative structure-activity relationship (QSAR) of a set of 35 flavonoid compounds presenting antioxidant activity was established by means of Genetic Algorithm-Multiple Linear Regression (GA-MLR) technique. Four-parametric models for two sets of data, the 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity $(R^2=0.788,\;Q^2_{cv}=0.699\;and\;Q^2_{ext}=0.577)$ and scavenging activity of reactive oxgen species (ROS) induced by $H_2O_2 (R^=0.829,\;Q^2_{cv}=0.754\;and\;Q^2_{ext}=0.573)$ were obtained with low external predictive ability on a mass basis, respectively. Each model gave some different mechanistic aspects of the flavonoid compounds tested in terms of the radical scavenging activity. Topological charge, H-bonding complex and deprotonation processes were likely to be involved in the radical scavenging activity.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2000년도 ITC-CSCC -1
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• pp.50-53
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• 2000

It is effectively removed noise in the image using FCNN(Fuzzy Cellular Neural Network) applying fuzzy theory to CNN(Cellular Neural Network) structure and HD(Hausdorff Distance) commonly used measures for object matching. HD calculates the distance between two point set of pixels in two-dimensional binary images without establishing correspondence. Also, this method is proposed in order to improve the operation speed. In this paper, $\alpha$-LTSHD(Least Trimmed Square HD) operator applying $\alpha$-Trimmed to LTSHD, one field of HD, is applied to FCNN structure, and it is proposed as the modified method in order to remove noise in the image. Also, it is made a comparison with the other filters by using MSE and SNR after removing noise using the FCNNS which are applied $\alpha$-LTSHD operator through the computer simulation. In a result, FCNN performance which is applied the proposed $\alpha$-LTSHD demonstrated the superiority to the other filters in the noise removal.

• 한국음향학회지
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• 제16권1호
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• pp.88-95
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• 1997

마이크로셀 환경인 개인통신망은 셀의 직경이 감소함으로써 매크로셀 환경인 셀룰라보다 더 많은 가입자를 수용할 수 있게 되었다. 이러한 환경 변화에 따라 개인통신망에 알맞은 채널 할당 기법이 필요하게 되었다. 본 논문에서는 셀루라의 채널 할당 방식을 개인통신망에 적용할 경우의 문제점을 분석하고 그러한 문제점을 극복하기 위하여 개인통신망에 알맞은 채널 할당 기법을 제안하였다. 제안된 기법의 효율성을 분석하고 다른 채널 할당 기법과 비교 평가하였다. 성능평가 파라미터는 호의 블록킹확률이다.

• Molecules and Cells
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• 제45권6호
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• pp.362-364
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• 2022

• Journal of Microbiology and Biotechnology
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• 제24권12호
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• pp.1744-1754
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• 2014

The hepatitis C virus (HCV) RNA genome is replicated by an RNA replicase complex (RC) consisting of cellular proteins and viral nonstructural (NS) proteins, including NS5B, an RNA-dependent RNA polymerase (RdRp) and key enzyme for viral RNA genome replication. The HCV RC is known to be associated with an intracellular membrane structure, but the cellular components of the RC and their roles in the formation of the HCV RC have not been well characterized. In this study, we took a proteomic approach to identify stomatin, a member of the integral proteins of lipid rafts, as a cellular protein interacting with HCV NS5B. Co-immunoprecipitation and co-localization studies confirmed the interaction between stomatin and NS5B. We demonstrated that the subcellular fraction containing viral NS proteins and stomatin displays RdRp activity. Membrane flotation assays with the HCV genome replication-competent subcellular fraction revealed that the HCV RdRp and stomatin are associated with the lipid raft-like domain of membranous structures. Stomatin silencing by RNA interference led to the release of NS5B from the detergent-resistant membrane, thereby inhibiting HCV replication in both HCV subgenomic replicon-harboring cells and HCV-infected cells. Our results identify stomatin as a cellular protein that plays a role in the formation of an enzymatically active HCV RC on a detergent-resistant membrane structure.

• 한국항행학회논문지
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• 제20권1호
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• pp.65-71
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• 2016

양자점 셀룰라 오토마타 (QCA; quantum-dot cellular automata)는 나노 규모의 크기와 낮은 전력 소비로 각광받고 있으며, CMOS 기술의 규모의 한계를 극복할 수 있는 대체 기술로 떠오르고 있다. 현재까지 QCA상에서 설계된 BCD-3초과 코드는 확장성을 고려하지 않았으며 대규모 회로 설계에는 적합하지 않았다. 이를 해결하기 위해 본 논문에서는 확장성을 고려한 BCD-3초과 코드 회로를 설계한다. 확장이 가능한 구조를 설계하기 위해 확장된 교차부 구조를 이용하여 입력과 출력의 흐름을 제어하고, 출력되는 값들의 동기화를 위해 5입력 다수결 게이트를 이용한다. 설계한 구조에 대해 QCADesigner를 이용하여 시뮬레이션을 수행한 후 그 결과에 대해 유효성을 검증한다. 제안된 구조는 기존의 URG BCD-3초과 코드변환기와 비교하여 32개의 게이트를 줄이며 빈 공간의 비율 또한 7% 감소시켰다. 또한 확장성이 고려되지 않은 기존의 QCA BCD-3초과 코드 변환기가 회로 확장 시 필요한 7개의 클럭을 1개의 클럭으로 줄였다.

• 예술인문사회 융합 멀티미디어 논문지
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• 제7권3호
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• pp.291-300
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• 2017

양자점 셀룰라 오토마타(QCA: quantum-dot cellular automata)는 셀룰라 오토마타와 유사하게 고안된 컴퓨팅 모델이며, 빠른 연산속도와 적은 전력손실로 차세대의 각광받는 기술도 떠오르고 있다. QCA는 최근 실험 결과와 함께 다양한 연구가 진행되고 있으며 나노 단위 소재로서 디바이스 밀도 및 상호 연결 문제를 해결할 수 있는 트랜지스터의 패러다임 중 하나이다. XOR(exclusive or) 게이트는 논리의 둘 중 하나가 참일 때 결과가 참이 되도록 작동하는 게이트이다. 제안하는 XOR 게이트는 5개의 층으로 구성되어 있다. 첫 번째 층은 OR 게이트, 세 번째 층과 다섯 번째 층은 AND 게이트로 구성되어 있고 중간에 두 번째 층과 네 번째 층은 통로로 구성하여 설계한다. 반가산기는 XOR 게이트와 AND 게이트로 이루어져 있다. 제안한 반가산기는 제안하는 XOR 게이트에서 셀 두 개를 추가하여 설계한다. 제안한 반가산기는 기존의 반가산기에 비해 보다 적은 수의 셀, 전체 면적, 그리고 클럭으로 구성한다.

• Molecules and Cells
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• 제43권10호
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• pp.841-847
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• 2020

Histone acetylation and deacetylation play central roles in the regulation of chromatin structure and transcription by RNA polymerase II (RNA Pol II). Although Hda1 histone deacetylase complex (Hda1C) is known to selectively deacetylate histone H3 and H2B to repress transcription, previous studies have suggested its potential roles in histone H4 deacetylation. Recently, we have shown that Hda1C has two distinct functions in histone deacetylation and transcription. Histone H4-specific deacetylation at highly transcribed genes negatively regulates RNA Pol II elongation and H3 deacetylation at inactive genes fine-tunes the kinetics of gene induction upon environmental changes. Here, we review the recent understandings of transcriptional regulation via histone deacetylation by Hda1C. In addition, we discuss the potential mechanisms for histone substrate switching by Hda1C, depending on transcriptional frequency and activity.