• 대한전기학회:학술대회논문집
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• 대한전기학회 1998년도 추계학술대회 논문집 학회본부 B
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• pp.612-614
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• 1998

As chip feature size decrease, interconnect delay gains more importance. A accurate timing analysis required to estimate interconnect delay as well as cell delay. In this paper, we present a timing-level delay calculation tool of which the accuracy is bounded within 10% of SPICE results. This delay calculation tool generates delay values in SDF(Standard Delay Format) for parasitic data extracted in SPEF(Standard Parasitic Exchange Format). The efficiency of the tool is easily seen because it uses AWE(Asymptotic Waveform Evaluation) algorithm for interconnect delay calculation, and precharacterized library and effective capacitance model for cell delay calculation.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1995년도 하계학술대회 논문집 B
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• pp.922-924
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• 1995

The modeling of accurate timing in storage elements of ASIC cell library was studied. The propagation delay time of clock signal affects the critical time and this can cause malfunction in the chip designed in synchronous. In this paper, an analysis on the effect of input slope of clock signal in timing modeling were carried out. For the first time, in ASIC design, the design guides that can be used in both $0.6{\mu}M$ and $0.8{\mu}m$ design rule were offered, reducing the run time of SPICE and the time of cell library development.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1993년도 하계학술대회 논문집 A
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• pp.450-452
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• 1993

In this study, a method is suggested to design the A/D conversion system which has high resolution to convert load cell signal. First, hardware was designed to reduce the offset voltage of integrator and comparator. And then, a calibration software technique was performed to obtain the stable data from A/D converter. The optimal parameters of each elements in the circuits was selected using the SPICE simulation. The main advantage of our method is high precision A/D converter can be constructed with low cost and high confidence. Therefore proposed method is expected to be used in the industrial field where a high precision measurement is required.

• BMB Reports
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• 제56권1호
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• pp.32-42
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• 2023

Heart disease is one of the major life-threatening diseases with high mortality and incidence worldwide. Several model systems, such as primary cells and animals, have been used to understand heart diseases and establish appropriate treatments. However, they have limitations in accuracy and reproducibility in recapitulating disease pathophysiology and evaluating drug responses. In recent years, three-dimensional (3D) cardiac tissue models produced using tissue engineering technology and human cells have outperformed conventional models. In particular, the integration of cell reprogramming techniques with bioengineering platforms (e.g., microfluidics, scaffolds, bioprinting, and biophysical stimuli) has facilitated the development of heart-on-a-chip, cardiac spheroid/organoid, and engineered heart tissue (EHT) to recapitulate the structural and functional features of the native human heart. These cardiac models have improved heart disease modeling and toxicological evaluation. In this review, we summarize the cell types for the fabrication of cardiac tissue models, introduce diverse 3D human cardiac tissue models, and discuss the strategies to enhance their complexity and maturity. Finally, recent studies in the modeling of various heart diseases are reviewed.

• 대한전자공학회논문지SD
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• 제45권8호
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• pp.1-10
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• 2008

본 논문에서는 새로운 스위칭 방식인 LFS (Local Field Switching)을 이용하여 설계한 128비트 MRAM (Magnetoresistive Random Access Memo교)에 대해 기술하였다. LFS 방식은 MTJ (Magnetic Tunnel Junction)를 직접 통과해 흐르는 전류에 의해 형성되는 국소 자기장을 이용하여 MTJ의 극성을 변환시킨다. 이 방식은 MTJ와 전류의 거리가 가깝기 때문에 작은 전류로도 충분히 큰 자기장을 형성하므로 writing current가 적어도 된다. 또한 Digit Line이 없어도 되므로 half select disturbance가 발생하지 않아 기존 MTJ를 이용한 방식에 비해 셀 선택도가 우수하다. 설계한 MRAM은 IT(트랜지스터)-1MTJ의 메모리 셀 구조를 가지며 양방향 write driver와 mid-point reference cell block, current mode sense amplifier를 사용한다. 그리고 MTJ 공정 없이 회로 동작을 확인하기 위해 LFS-MTJ cell을 CMOS emulation cell로 대체하였다. 설계한 회로를 6 metal을 사용하는 $0.18{\mu}m$ CMOS 공정으로 구현하였고 제작된 chip을 custom board 상에서 테스트하여 동작을 확인하였다.

• 동의생리병리학회지
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• 제17권2호
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• pp.529-541
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• 2003

Yukmijihwang-tang has been widely used as an and-aging herbal medicine for hundred years in Asian countries. Numerous studies show that Yukmijihwangtang has anti-oxidative effect both in vivo and in vitro. It has been reported that Moutan Cortex Radicis extract (MCR) was the most effective herb in Yukmijihwang-tang on undifferentiated PC12 cells upon oxidative-stressed with hydrogen peroxide. The purpose of this study is to; 1) evaluate the recovery of neuronal damage by assessing the anti-oxidant effect of MCR on PC12 cells differentiated with nerve growth factor (NGF), 2) identify candidate genes responsible for anti-oxidative effect on differentiated PC12 cells by oligonucleotide chip microarray. PC12 cells, which were differentiated by treating with NGF, were treated without or with hydrogen peroxide in the presence or absence of various concentration of MCR. Cell survival was determined by using MTS assay. Measurement of intracellular reactive oxygen species (ROS) generation was determined using the H2DCFDA assay The viability of cells treated with MCR was significantly recovered from stressed PC12 cell. In addition, wide rage of concentrations of MCR shows dose-dependent inhibitory effect on ROS production in oxidative-stressed cells. Total RNAs of cells without treatment(Control group), only treated with H₂O₂ (stressed group) and treated with both H₂O₂ and of MCR (MCR group) were isolated, and cDNAs was synthesized using oligoT7(dT) primer. The fragmented cRNAs, synthesized from cDNAs, were applied to Affymetrix GeneChip Rat Neurobiology U34 Array. mRNA of Calcium/calmodulin-dependent protein kinase II delta subunit(CaMKII), neuron glucose transporter (GLUT3) and myelin/oligodendrocyte glycoprotein(MOG) were downregulated in Stressed group comparing to Control group. P2X2-5 receptor (P2X2R-5), P2X2-4 receptor (P2X2R-4), c-fos, 25 kDa synaptosomal attachment protein(SNAP-25a) and GLUT3 were downregulated, whereas A2 adenosine receptor (A2AR), cathechol-O-methyltransferase(COMT), glucose transporter 1 (GLUT1), EST223333, heme oxygenase (HO), VGF, UI-R-CO-ja-a-07-0-Ul.s1 and macrophage migration inhibitory factor (MIF) were upregulated in MCA group comparing to Control group. Expression of Putative potassium channel subunit protein (ACK4), P2X2A-5, P2X2A-4, Interferon-gamma inducing factor isoform alpha precursor (IL-18α), EST199031, P2XR, P2X2 purinoceptor isoform e (P2X2R-e), Precursor interleukin 18 (IL-18) were downregulated, whereas MOO, EST223333, GLUT-1, MIF, Neuronatin alpha, UI-R-C0-ja-a-07-0-Ul.s1, A2. adenosine receptor, COMT, neuron-specific enolase (NSE), HO, VGF, A rat novel protein which is expressed with nerve injury (E12625) were upregulated in MCR group comparing to Stressed group. The results suggest that decreased viability and AOS production of PC12 cell by H₂O₂ may be, at lease, mediated by impaired glucose transporter expression. It is implicated that the MCR treatment protect PC12 cell from oxidative stress via following mechanisms; improving glucose transport into the cell, enhancing expression of anti-oxidative genes and protecting from dopamine cytotoxicity by increment of COMT and MIF expression. The list of differentially expressed genes may implicate further insight on the action and mechanism behind the anti-oxidative effects of herbal extract Moutan Cortex Radicis.

• 한국식품위생안전성학회지
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• 제33권1호
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• pp.50-57
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• 2018

Salmonella는 전세계적으로 식중독을 유발하는 주요 원인 균으로서, 식중독을 유발하는 Salmonella를 신속하게 검출하는 방법은 식품 안전을 위한 중요한 도구이다. Real-time PCR은 식중독균을 검출하기 위한 신속검사법으로 널리 사용되어 왔다. 최근에는 NBS LabChip real-time PCR이라는 새로운 시스템이 칩타입으로 조작이 간편하며 초고속의 real-time PCR 시스템이라는 보고가 있었다. 본 연구에서는 살모넬라의 신속한 검출을 위하여 NBS LabChip real-time PCR에 기반하여 real-time PCR 반응 시간이 20분 이내인 검출법을 확인하고자 하였다. 프라이머와 프로브 설계를 위해 두 개의 타겟 유전자(invA, stn)가 선택되었으며, 특이도와 민감도(검출한계)를 평가함으로 개발된 검출법을 검증하고자 하였다. 본 연구에서는 특이도 검증을 위해 Salmonella 균주 42주와 Non-Salmonella 균주 21주를 포함하였으며, 본 방법으로 Salmonella 42주에 대해서만 정확하게 검출이 가능하였다. 검출한계는 살모넬라 genome DNA 기준으로 $10^1copies/{\mu}L$으며, 소시지에서는 4시간 증균 이후 접종균수로서 $10^1CFU/g$에서 $10^2CFU/g$까지 검출이 가능하였다. 본 연구에서 개발된 검출법은 신속한 식중독 원인조사에 활용될 수 있을 것으로 기대된다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권5호
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• pp.2519-2525
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• 2016

There are several existing reports of microarray chip use for assessment of altered gene expression in different diseases. In fact, there have been over 1.5 million assays of this kind performed over the last twenty years, which have influenced clinical and translational research studies. The most commonly used DNA microarray platforms are Affymetrix GeneChip and Quality Control Software along with their GeneChip Probe Arrays. These chips are created using several quality controls to confirm the success of each assay, but their actual impact on gene expression profiles had not been previously analyzed until the appearance of several bioinformatics tools for this purpose. We here performed a data mining analysis, in this case specifically focused on ovarian cancer, as well as healthy ovarian tissue and ovarian cell lines, in order to confirm quality control results and associated variation in gene expression profiles. The microarray data used in our research were downloaded from ArrayExpress and Gene Expression Omnibus (GEO) and analyzed with Expression Console Software using RMA, MAS5 and Plier algorithms. The gene expression profiles were obtained using Partek Genomics Suite v6.6 and data were visualized using principal component analysis, heat map, and Venn diagrams. Microarray quality control analysis showed that roughly 40% of the microarray files were false negative, demonstrating over- and under-estimation of expressed genes. Additionally, we confirmed the results performing second analysis using independent samples. About 70% of the significant expressed genes were correlated in both analyses. These results demonstrate the importance of appropriate microarray processing to obtain a reliable gene expression profile.

• Asian-Australasian Journal of Animal Sciences
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• 제20권10호
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• pp.1557-1566
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• 2007

Ten male crossbred (Thai $Native{\times}Anglo$ Nubian) goats with average live weight of $27{\pm}2$ kg were randomly assigned according to a $5{\times}5$ replicated Latin square design to receive five diets, $T_1$ = concentrate with 0% cassava chip (CC), $T_2$ = 25% CC, $T_3$ = 50% CC, $T_4$ = 75% CC and $T_5$ = 100% CC. Fresh elephant grass (FEG) was offered ad libitum as the roughage. A metabolism trial lasted for 21 days during which liveweight changes and feed intakes were measured. Based on this experiment, there were no significant differences (p>0.05) among treatment groups regarding DM intake and digestion coefficients of nutrients (DM, OM, NDF and ADF), except for $T_5$ (100% CC) which was lowest (p<0.05) in digestion coefficient of CP than $T_1$ and $T_3$. Rumen parameters (ruminal pH, $NH_3$-N and volatile fatty acids), blood urea nitrogen, blood glucose and packed cell volume were similar among treatments. Moreover, rumen microorganism populations were not affected (p>0.05) by cassava inclusion. The amount of N absorption and retention were similar among treatments, except for $T_5$ which tended to be slightly lower. Based on this experiment, it could be concluded that the optimal level of cassava inclusion to replace corn in goat diets was in the range of 25-75% of CC when fed with FEG and it was a good approach in exploiting the use of local feed resources for goat production.

• 한국콘텐츠학회논문지
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• 제7권1호
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• pp.40-47
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• 2007

단백질 매크로 어레이 영상에서 단백질 칩 각각의 특징을 규명하는 것은 중요한 것이다. 사람의 시각에 의한 판단은 많은 단백질 칩 영상을 실험할 경우, 장시간의 관찰과 그로 인한 오류가 발생할 수 있다. 따라서 시뮬레이터를 통한 특성 파악이 필요하게 되고, 매크로 어레이 스캔 영상에 대해 특성 분석을 할 경우 효율을 극대화할 수 있다. 형광 스캔 영상에 있어서, 각 셀의 반응도는 컬러 영상의 R, G, B 분포에 의존하여 왔다. 그러나 중첩되는 영상의 경우는 한쪽으로 구분하여 분류하기가 어렵다. 본 논문은 이러한 단점을 극복하기 위해 사용자가 원하는 색상에 대한 퍼지 측도 값을 적용한 퍼지 적분 값으로서 단백질 칩의 반응색상을 구분 지었다. Scan Array 5000에 의해 구성된 매크로 어레이 형광 영상들에 대해 실험한 결과, 퍼지 적분을 사용한 제안 방법이 모호한 색상에 대해 결정을 내릴 수 있는 요소가 됨을 보여 주었다.