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Effect of Moutan Cortex Radicis on gene expression profile of differentiated PC12 rat cells oxidative-stressed with hydrogen peroxide  

Kim Hyun Hee (Department of Oriental Medicine, Graduate School of Kyunghee University)
Rho Sam Woong (Department of Oriental Medicine, Graduate School of Kyunghee University)
Na Youn Gin (Purimed R&D Institute)
Bae Hyun Su (Department of Oriental Medicine, Graduate School of Kyunghee University)
Shin Min Kyu (Department of Oriental Medicine, Graduate School of Kyunghee University)
Kim Chung Suk (Korea Institute of Oriental Medicine)
Hong Moo Chang (Department of Oriental Medicine, Graduate School of Kyunghee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.17, no.2, 2003 , pp. 529-541 More about this Journal
Abstract
Yukmijihwang-tang has been widely used as an and-aging herbal medicine for hundred years in Asian countries. Numerous studies show that Yukmijihwangtang has anti-oxidative effect both in vivo and in vitro. It has been reported that Moutan Cortex Radicis extract (MCR) was the most effective herb in Yukmijihwang-tang on undifferentiated PC12 cells upon oxidative-stressed with hydrogen peroxide. The purpose of this study is to; 1) evaluate the recovery of neuronal damage by assessing the anti-oxidant effect of MCR on PC12 cells differentiated with nerve growth factor (NGF), 2) identify candidate genes responsible for anti-oxidative effect on differentiated PC12 cells by oligonucleotide chip microarray. PC12 cells, which were differentiated by treating with NGF, were treated without or with hydrogen peroxide in the presence or absence of various concentration of MCR. Cell survival was determined by using MTS assay. Measurement of intracellular reactive oxygen species (ROS) generation was determined using the H2DCFDA assay The viability of cells treated with MCR was significantly recovered from stressed PC12 cell. In addition, wide rage of concentrations of MCR shows dose-dependent inhibitory effect on ROS production in oxidative-stressed cells. Total RNAs of cells without treatment(Control group), only treated with H₂O₂ (stressed group) and treated with both H₂O₂ and of MCR (MCR group) were isolated, and cDNAs was synthesized using oligoT7(dT) primer. The fragmented cRNAs, synthesized from cDNAs, were applied to Affymetrix GeneChip Rat Neurobiology U34 Array. mRNA of Calcium/calmodulin-dependent protein kinase II delta subunit(CaMKII), neuron glucose transporter (GLUT3) and myelin/oligodendrocyte glycoprotein(MOG) were downregulated in Stressed group comparing to Control group. P2X2-5 receptor (P2X2R-5), P2X2-4 receptor (P2X2R-4), c-fos, 25 kDa synaptosomal attachment protein(SNAP-25a) and GLUT3 were downregulated, whereas A2 adenosine receptor (A2AR), cathechol-O-methyltransferase(COMT), glucose transporter 1 (GLUT1), EST223333, heme oxygenase (HO), VGF, UI-R-CO-ja-a-07-0-Ul.s1 and macrophage migration inhibitory factor (MIF) were upregulated in MCA group comparing to Control group. Expression of Putative potassium channel subunit protein (ACK4), P2X2A-5, P2X2A-4, Interferon-gamma inducing factor isoform alpha precursor (IL-18α), EST199031, P2XR, P2X2 purinoceptor isoform e (P2X2R-e), Precursor interleukin 18 (IL-18) were downregulated, whereas MOO, EST223333, GLUT-1, MIF, Neuronatin alpha, UI-R-C0-ja-a-07-0-Ul.s1, A2. adenosine receptor, COMT, neuron-specific enolase (NSE), HO, VGF, A rat novel protein which is expressed with nerve injury (E12625) were upregulated in MCR group comparing to Stressed group. The results suggest that decreased viability and AOS production of PC12 cell by H₂O₂ may be, at lease, mediated by impaired glucose transporter expression. It is implicated that the MCR treatment protect PC12 cell from oxidative stress via following mechanisms; improving glucose transport into the cell, enhancing expression of anti-oxidative genes and protecting from dopamine cytotoxicity by increment of COMT and MIF expression. The list of differentially expressed genes may implicate further insight on the action and mechanism behind the anti-oxidative effects of herbal extract Moutan Cortex Radicis.
Keywords
Moutan Cortex Radicis; Yukmijihwangtang; anti-oxidative effect; PC12 cell; oligonucelotide chip microarray; anti-aging;
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  • Reference
1 Interleukin-18: a novel cytokine that augments both innate and acquired immunity. /
[ Okamura H.;Tsutsui H.;Kashiwamura S.;Yoshimoto T.;Nakanishi K. ] / Adv lmmunol   DOI
2 Intracellular calcium and arachidonic acid increase SNAP-25 expression in cultured rat hippocampal explants, but not in cultured rat cerebellar explants. /
[ Sepulveda C.M.;Troncoso C.C.;Lara H.;Cardenas A.M. ] / Neurosci Lett   DOI
3 Syntaxin 13 is a developmentally regulated SNARE involved in neurite outgrowth and endosomal trafficking. /
[ Hirling H.;Steiner P.;Chaperon C.;Marsault R.;Regazzi R.;Catsicas S. ] / Eur J Neurosci   DOI
4 Direct evidence for the coupling of A2-adenosine receptor to stimulatory guanine nucleotide-binding-protein in bovine brain striatum. /
[ Marala R. B.;Mustafa S. J. ] / J Pharmacol Exp Ther
5 Definition of subclasses of adenosine receptors associated with adenyl ate cyclase: interaction of adenosine analogs with inhibitory Al receptors and stimulatory A2 receptors. /
[ Ukena D.;Olsson R. A.;Daly J. W. ] / Can J Physiol Pharmacol   DOI
6 Anti-ischemia reperfusion damage and anti-lipid peroxidation effects of paeonol in rat heart. /
[ Zhang W. G.;Zhang Z. S. ] / Yao Xue Xue Bao
7 Inhibitory effects of paeonol on experimental atherosclerosis and platelet aggregation of rabbit. /
[ Shi L.;Fan P. S.;Fang J. X.;Han Z. X. ] / Zhongguo Yao Li Xue Bao
8 Studies on the mechanism of antiaggregatory effect of Moutan Cortex. /
[ Hirai A.;Terano T.;Hamazaki T.;Sajiki J.;Saito H.;Tahara K;Tamura Y.;Kumagai A. ] / Thromb Res Suppl   DOI
9 Pharmacological studies on the root bark of Paeonia moutan. II. Anti-inflammatory effect, preventive effect on stress-induced gastric erosion, inhibitory effect on gastric juice secretion and other effects of paeonol. /
[ Harada M.;Yamashita A.;Aburada M. ] / Yakugaku Zasshi   DOI
10 Macrophage migration inhibitory factor (MIF): mechanisms of action and role in disease. /
[ Lue H.;Kleemann R.;Calandra T.;Roger T.;Bernhagen J. ] / Microbes Infect   DOI
11 Identification of macrophage migration inhibitory factor(MIF) in rat spinal cord and its kinetics on experi mental spinal cord injury. /
[ Fujimoto S. ] / Hokkaido Igaku Zasshi
12 Protection of intracellular dopamine cytotoxicity by dopamine disposition and metabolism factors. /
[ Weingarten P.;Zhou Q. Y. ] / J Neurochem   DOI
13 A site accessible to extracellular TEA + and K + influences intracellular Mg2+ block of cloned potassium channels. /
[ Ludewig U.;Lorra C.;Pongs O.;Heinemann S. H. ] / Eur Biophys J
14 Caspase-1 processes IFN-gamma inducing factor and regulates LPS-¬induced IFN-gamma production. /
[ Ghayur T.;Banerjee S.;Hugunin M.;Butler D.;Herzog L.;Carter A.;Quintal L.;Sekut L.;Talanian R.;Paskind M.;Wong W.;Kamen R.;Tracey D.;Allen H. ] / Nature   DOI
15 Cloning of a new cytokine that induces IFN-gamma production by T cells. /
[ Okamura H.;Tsutsui H.;Komatsu T.;Yutsudo M.;Hakura A.;Tanimoto T.;Torigoe K.;Okura T.;Nukada Y.;Hattori K.;Akita K.;Namba M.;Taanbe F.;Konishi K.;Fukuda S.;Kurimoto M. ] / Nature   DOI
16 Mechanisms of intracellular protein transport. /
[ Rothman J.E. ] / Nature   DOI
17 /
[ 하북의학원(校釋) ] / 靈樞經校釋(下冊)
18 노화과정의 흰쥐에서 보신환이 신장의 대사효소계에 미치는 영향 /
[ 손민성;오민석;송태원 ] / 대전대논문집
19 오자지황음자가 노화백서의 혈청변화와 혈청<TEX>$\cdot$</TEX>뇌조직의 항산화활성에 미치는 영향 /
[ 서경석;이상룡 ] / 동의정신과학회지
20 左歸飮과 右歸飮이 노화 Rat의 腦 과산화지질 생성 및 활성산소 생성계 효소 활성에 미치는 영향 /
[ 윤철호;정지천;박선동 ] / 대한한의학회지
21 左歸飮과 右歸飮이 노화 Rat의 肝 과산화지질 생성 및 활성산소 생성계 효소 활성에 미치는 영향 /
[ 윤철호;정지천 ] / 대한한의학회지
22 /
[ 徐舜圭 ] / 成人病<TEX>$\cdot$</TEX>老人病學
23 /
[ 김호철 ] / 한약약리학
24 pt point mutation in pIp gene results in hyperexpression of MOG in hypomyelinated rabbit. /
[ Sypecka J. ] / Acta Neurobiol Exp
25 Induction of EAE in mice with recombinant human MOG, and treatment of EAE with a MOG peptide. /
[ Devaux B.;Enderlin F.;Wallner B.;Smilek D. E. ] / J Neuroimmunol   DOI
26 MAG and MOG enhance neurite outgrowth of embryonic mouse spinal cord neurons. /
[ Turnley AM;Bartlett PF. ] / Neuroreport   DOI
27 ATP-stimulated activation of the mitogen-activated protein kinases through ionotrophic P2X2 purinoreceptors in PC12 cells; Difference in purinoreceptor sensitivity in two PC12 cell lines. /
[ Swanson K. D.;Reigh C.;Landreth G. E. ] / J Biol Chem   DOI
28 Identification of Fos target genes by the use of selective induction systems. /
[ Braselmann S.;Bergers G.;Wrighton C.;Graninger P.;Superti-Furga G.;Busslinger M. ] / J Cell Sci Suppl
29 Early response genes as markers of neuronal activity and growth factor action. /
[ Sagar S. M.;Sharp F. R. ] / Adv Neurol
30 C-fos mediates cocaine inhibition of NGF-induced PC12 cell differentiation. /
[ Zachor D. A.;Moore J. F.;Jin J.;Theibert A. B.;Percy A K. ] / Molecular Genetic Metabolism   DOI
31 /
[ 王琦;李炳文;王慶基;彭榮琛(編著) ] / 黃帝內經素問今釋
32 The identification of a novel synaptosomal-associated protein, SNAP-25, differentially expressed by neuronal subpopulations. /
[ Oyler G. A.;Higgins G. A.;Hart R. A.;Battenberg E.;Billingsley M.;Bloom F. E.;Wilson M. C. ] / J Cell Biol   DOI
33 The t-SNAREs syntaxin 1 and SNAP-25 are present on organelles that participate in synaptic vesicle recycling. /
[ Walch-Solimena C.;Blasi J.;Edelmann L.;Chapman E. R.;von Mol-lard G. F.;Jahn R. ] / J Cell Biol   DOI
34 /
[ 리정복 ] / 장수학
35 /
[ 두호경(編著) ] / 東醫腎系學
36 Free radical theory of aging. /
[ Harman D. ] / Mutat Res   DOI
37 숙지황과 육미지황탕이 노화과정 흰쥐에서의 항산화 기전에 미치는 영향. /
[ 안상원;이철완 ] / 대전대논문집
38 六味地黃湯加味方이 흰쥐의 기억능력과 중추신경계 유전자 발형에 미치는 영향 /
[ 최보업;배현수;신민규;홍무창 ] / 동의생리병리학회지
39 六味地黃湯 構成藥物이 PC12 細胞의 酸化抑制에 미치는 영향 /
[ 서영은 ] / 석사학위논문
40 牧丹皮가 Collagen 유발 관절염에 미치는 영향 /
[ 김동혁;송봉근;김형균 ] / 전통의학회지
41 A study of paeonol injection on immune functions in rats. /
[ Li F. C.;Zhou X. L.;Mao H. L. ] / Zhongguo Zhong Xi Yi Jie He Za Zhi
42 Lysophosphatidic Acid and Apoptosis of nerve Growth Factor-differentiated PC-12 cells. /
[ Holtsberg F.W.;Steiner M.R.;Bruce-Keller A.J.;Keller J.N.;Mattson M.P.;Moyers J.C.;Steiner S.M. ] / J. Neurosc. Res   DOI
43 /
[ 한국의약품수출입협회;한국의약품시험연구소 ] / 한약(생약)규격집
44 a novel acetylcholine-&not;sterase inhibitor, attenuates hydrogen peroxide-induced injury in PC12 cells. /
[ Zhang H.Y.;Tang X.C.;Huperzine B. ] / Neurosci Lett   DOI
45 Interleukin-18 is induced in acute inflammatory demyelinating polyneuropathy. /
[ Jander S.;Stoll G. ] / J Neuro-&not;immunol
46 Induction of the proinflamma&not;-tory cytokine interleukin-18 by axonal injury. /
[ Menge T.;Jander S.;Stoll G. ] / J Neurosci Res   DOI
47 Upstream and intron regulatory regions for expression of the rat neuron-specific enolase gene. /
[ Sakimura K.;Kushiya E.;Ogura A.;Kudo Y.;Katagiri T.;Takahashi Y. ] / Mol Brain Res   DOI
48 Increased levels of neuron-specific enolase in PC12 pheochromocytoma cells as a result of nerve growth factor treatment. /
[ Vinores S. A.;Marangos P. J.;Parma A. M.;Guroff G. ] / J Neurochem   DOI
49 Enhanced heme oxygenase activity increases the antioxidant defense capacity of guinea pig liver upon acute cobalt chloride loading: comparison with rat liver. /
[ Christova T. Y.;Duridanova D. B.;Setchenska M. S. ] / Comp Biochem Physiol C Toxicol Pharmacol   DOI
50 Brain catecholamine metabolism in catechol-O&not;methyltransferase (COMT)-deficient mice. /
[ Huotari M.;Gogos J. A.;Karayiorgou M.;Koponen O.;Forsberg M.;Raasmaja A.;Hyttinen J.;Mannisto P. T. ] / Eur J Neurosci   DOI
51 The heme oxygenase system and cellular defense mechanisms. Do HO-1 and HO-2 have different functions? /
[ Maines M. D.;Panahian N. ] / Adv Exp Med Biol   DOI
52 Fundamental role of heme oxygenase in the protection against ischemic acute renal failure. /
[ Akagi R.;Takahashi T.;Sassa S. ] / JPN J Pharmacol   DOI
53 VGF: a novel role for this neuronal and neuroendocrine polypeptide in the regulation of energy balance. /
[ Salton S. R.;Ferri G. L.;Hahm S.;Snyder S. E.;Wilson A. J.;Possenti R.;Levi A. ] / Front Neuroendocrinol   DOI
54 Segment-specific expression of the neuronatin gene during early hindbrain development. /
[ Wijnholds J.;Chowdhury K.;Wehr R.;Gruss P. ] / Dev Biol   DOI
55 Neuronatin mRNA in PC12 cells: downregulation by nerve growth factor. /
[ Joseph R.;Tsang W.;Dou D.;Nelson K.;Edvardsen K. ] / Brain Res   DOI
56 Macrophage migration inhibitory factor (MIF) modulates innate immune responses induced by endotoxin and Gram-negative bacteria. /
[ Roger T.;Glauser M. P.;Calandra T. ] / J Endotoxin Res   DOI
57 Expression of purinergic P2X2 receptor-channels and their role in calcium signaling in pituitary cells. /
[ Stojilkovic S. S.;Tomic M.;Van Goor F.;Koshimizu T. ] / Biochem Cell Biol   DOI
58 Differential induction of interleukin-12, interleukin-18, and interleukin-1 beta converting enzyme mRNA in experimental autoimmune encephalomyelitis of the Lewis rat. /
[ Jander S.;Stoll G. ] / J Neuroimmunol   DOI
59 Neutralizing antibodies to IFN-gamma-inducing factor prevent experimental autoimmune encephalomyelitis. /
[ Wildbaum G.;Youssef S.;Grabie N.;Karin N. ] / J Ilmmunol
60 Neuron-specific glucose transporter(NSGT): CNS distribution of GLUT3 rat glucose transporter(RGT3) in rat central neurons. /
[ Nagamatsu S.;Sawa H.;Kamada K.;Nakamichi Y.;Yoshimoto K.;Hoshino T. ] / FEBS lett   DOI
61 Mechanical allodynia caused by intraplantar injection of P2X receptor agonist in rats: involvement of heteromeric P2X2/3 receptor signaling in capsaicin-insensitive primary afferent neurons. /
[ Tsuda M.;Koizumi S.;Kita A.;Shigemoto Y.;Ueno S.;Inoue K. ] / J Neurosci
62 Localization of ATP-gated P2X2 and P2X3 receptor immunoreactive nerves in rat taste buds. /
[ Bo X.;Alavi A.;Xiang Z.;Oglesby I.;Ford A.;Burnstock G. ] / Neuroreport   DOI
63 Functional role of alternative splicing in pituitary P2X2 receptor-channel activation and desensitization. /
[ Koshimizu T.;Tomic M.;Van Goor F.;Stojilkovic S. S. ] / Mol Endocrinol   DOI
64 Extracellular ATP inhibits starvation-induced apoptosis via P2X2 receptors in differentiated rat pheochromocytoma PC12 cells. /
[ Fujita N.;Kakimi M.;Ikeda Y.;Hiramoto T.;Suzuki K. ] / Life Science   DOI
65 NGF enhances depolarization effects on SNAP-25 expression: induction of SNAP-25b isoform. /
[ Hepp R;Dupont JL;Aunis 0;Langley K;Grant NJ. ] / Neuroreport   DOI