• Journal of the Optical Society of Korea
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• 제13권2호
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• pp.223-226
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• 2009

A Magneto-Optical Trap (MOT) for $^{87}Rb$ atoms near the surface of a dielectric coated mirror at the top of a small $20{\times}25{\times}40\;mm^3$ cell has been observed. Two beams of $3.3\;mW/cm^2$ were used for optical cooling and an anti-Helmholtz magnetic field with a spatial gradient of 9.1 G/cm was used for magnetic trapping. The thickness of the mirror coated on a cover glass was less than $100{\mu}m$. The mirror covered the top of a cell and the atom-chip was located outside the vacuum in order to exploit the long life time of the mirror and easy operation of the chip. The trapping position was found 5 mm beneath the mirror surface. The number of trapped atoms was roughly $3{\times}10^7$ atoms and the temperature was approximately a few tens mK. In this paper, we describe the construction of the mirror-MOT in detail.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2000년도 International Symposium on Bioinformatics
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• pp.59-60
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• 2000

The past few years have seen a dramatic increase in gene expression data on the basis of DNA microarrays or DNA chips. Going beyond a generic view on the genome, microarray data are able to distinguish between gene populations in different tissues of the same organism and in different states of cells belonging to the same tissue. This affords a cell-wide view of the metabolic and regulatory processes under different conditions, building an effective basis for new diagnoses and therapies of diseases. In this talk we present machine learning techniques for effective mining of DNA microarray data. A brief introduction to the research field of machine learning from the computer science and artificial intelligence point of view is followed by a review of recently-developed learning algorithms applied to the analysis of DNA chip gene expression data. Emphasis is put on graphical models, such as Bayesian networks, latent variable models, and generative topographic mapping. Finally, we report on our own results of applying these learning methods to two important problems: the identification of cell cycle-regulated genes and the discovery of cancer classes by gene expression monitoring. The data sets are provided by the competition CAMDA-2000, the Critical Assessment of Techniques for Microarray Data Mining.

• 센서학회지
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• 제14권4호
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• pp.237-243
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• 2005

Strain gauge type load cell is used widely as weight sensor. However, it has problems such as noise, power consumption, high cost and big size. Semiconductor type piezoresistive pressure sensor is practically used in recent for low hysteresis, good linearity, small size, light weight and strong on vibration. In this paper, we have fabricated the piezoresistive pressure sensor and packaged the miniature weight sensor. We packaged the miniature weight sensor by flip-chip bonding between die and PCB for durability, because the weight sensor is directly contacted on a physical solid distinct from air and oil pressure. We measured the characteristics of the weight sensor, which had the output of $10{\sim}80$ mV on the weight range of $0{\sim}2$ kg. In the result, we could fabricate the weight sensor with an accuracy of 3 %FSO linearity.

• 대한한의학방제학회지
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• 제29권4호
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• pp.267-275
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• 2021

Objectives : Previously, we reported that compound K isolated from fermented ginseng by Aspillus oryzae has a wide biochemical and pharmacological effect, including anti-cancer activity in prostate cancer PC-3 cells. Despite these findings, its signaling pathway and gene expression pattern are not clearly understood. Methods : To confirm the gene expression study of treated with compound K in PC-3 cells, a cDNA microarray chip composed of 44K human cDNA probes was used. MTT assay, western blot analysis, propidium iodide staining, and annexin V/propidium iodide staining were analyzed. Results : We confirmed the differences of gene expression profiles. Then, we analyzed with the cell cycle arrest, cell death and cell proliferation related genes using DAVID database. Conclusions : Our finding should be useful for understanding genome-wide expression patterns of compound K-mediated cell cycle arrest toward induction of cell death and be helpful for finding future cancer therapeutic targets for prostate cancer cells.

• 대한전자공학회논문지SP
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• 제44권5호
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• pp.103-111
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• 2007

본 논문에서는 single-chip CMOS Image Sensor(CIS)용 고화질 image signal processor(ISP)에 최적화된 하드웨어 구조를 제안한다. Single-chip CIS는 CIS와 ISP가 하나의 칩으로 구현된 것으로, 다양한 휴대기기에 사용된다. 휴대기기의 특성상, single-chip CIS용 ISP는 고화질이면서도 저전력을 위해 하드웨어 복잡도를 최소화해야 한다. 영상의 품질 향상을 위해서 다양한 영상 처리 블록들이 ISP에 적용되지만, 그 중에 핵심이면서 하드웨어 복잡도가 가장 큰 블록은 컬러 영상을 만들기 위한 색 보간 블록과 영상을 선명하게 하기 위한 화질 개선 필터 블록이다. 이들 블록은 데이터 처리를 위한 로직 외에도 라인 메모리를 필요로 하기 때문에 ISP의 하드웨어 복잡도의 대부분을 차지한다. 기존 ISP에서는 색 보간과 화질 개선 필터를 독립적으로 수행하였기 때문에 많은 수의 라인 메모리가 필요하였다. 따라서 하드웨어 복잡도를 낮추기 위해서는 낮은 성능의 색보간 알고리즘을 적용하거나, 화질 개선 필터를 사용하지 않아야 했다. 본 논문에서는 화질 개선을 위해 경계 적응적이면서 채널간 상관관계를 고려하는 고화질 색 보간 알고리즘을 적용하였다. 또한 채널 간 상관관계를 고려하는 색 보간 알고리즘의 특성을 이용하여 색 보간 블록과 화질 개선 필터 블록이 라인 메모리를 공유하도록 설계함으로써, 전체 라인 메모리 수를 최소화하는 새로운 구조를 제안한다. 제안된 방법을 적용하면 화질 개선 필터 블록을 위한 추가적인 라인 메모리가 불필요하기 때문에, 고화질과 낮은 복잡도 모두를 만족시킬 수 있다. 제안 방식과 기존 방식의 MSE(Mean Square Error)는 0.37로, 메모리 공유로 인한 화질의 저하는 거의 없었고, 고화질 색 보간 알고리즘을 적용했기 때문에 전체적인 화질은 향상되었다. 제안된 ISP 구조는 Verilog HDL 및 FPGA를 이용하여 실시간으로 구현 검증되었다. 0.25um CMOS 표준 셀 라이브러리를 이용하여 합성하였을 때, 총 게이트 수는 37K개였으며 7.5개의 라인 메모리가 사용되었다.

• 센서학회지
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• 제17권4호
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• pp.245-249
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• 2008

This paper presents a microfluidic cell sizing method using hydrophoretic size-based separation. By exploiting slanted obstacles in a microchannel, we can generate a lateral pressure gradient so that microparticles can be deflected and arranged along lateral flows induced by the gradient. Using such movement of particles, we discriminated 8 to 15 μm-sized beads. We measured the size of U937 cells by comparing the hydrophoretic response of the cells to those of the size-standard beads whose diameters are known. Due to its simple design and fabrication, the sizing method can be easily integrated with other microfluidic components such as cell culture chambers conducting on-chip sizing and sorting.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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• pp.84-84
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• 2013

FcBP consisting of 13 amino acids specifically binds to Immunoglobulin G Fc domain. Initially, we utilized this peptide for preparation of antibody chip as a PEG composite for enhanced solubility. After then, the peptide conjugate was immobilized on agarose resin, resulting in highly efficient affinity column for antibody purification. The efficiency was comparable to commercial Protein A column. Recently, this peptide was conjugated with cell penetratingpeptide (CPP) on a backbone of GFP, affording antibody transducer, which carries antibody into live cells by simple mixing of antibody and the transducer in cell culture media. Antibody transduction into cells was monitored by live cell imaging. More recently, the FcBP was fused to ferritin cage, which consists of 24 ferritin protein molecules. The FcBP-ferritin cage showed greatly increased binding affinity to human IgG. Its binding was analyzed by QCM and SPR analysis. Finally, it was selectively delivered by Herceptin to SKBR3, a breast cancer cell, over MCF10A, non-tumorigenic cells.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.1450-1454
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• 2008

In the conventional biology, the most of cell studies was carried out by culturing cells in the Petri dish and by investigating cellular behavior under the diverse bio-molecule (cell signalling materials, drugs or etc.) conditions. However, in vivo environments, diverse stimulations including chemical, mechanical and topological environments involved in the proliferation, differentiation and migration of cells and it is almost impossible to provide these conditions with traditional method. We have developed the methods to provide the well defined chemical and mechanical stimulations using microfluidic devices and applied these approaches to the study of environmental effect on cells. In this paper, we will introduce our microfluidic chips to provide microenvironment and its applications using several cells.

• Bulletin of the Korean Chemical Society
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• 제34권4호
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• pp.1175-1180
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• 2013

A microfabricated electrochemical cell comprising PDMS-based microchannel and in-channel gold microelectrodes was fabricated as a sensitive and a miniature alternative to the conventional electroanalytical systems. A reproducible fabrication procedure enabled patterning of multiple microelectrodes integrated within a PDMS-based fluidic network. The active area of each electrode was $200{\mu}m{\times}200{\mu}m$ with a gap of $200{\mu}m$ between the electrodes which resulted in a higher signal to noise ratio. Also, the PDMS layer served the purpose of shielding the electrical interferences to the measurements. Analytes such as potassium ferrocyanide; amino acid: cysteine and nucleoside: guanosine were characterized using the fabricated cell. The microchip was comparable to bulk electrochemical systems and its applicability was also demonstrated with flow injection based rapid amperometric detection of DNA samples. The device so developed shall find use as a disposable electrochemical cell for rapid and sensitive analysis of electroactive species in various industrial and research applications.

• 대한전자공학회논문지
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• 제23권4호
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• pp.557-564
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• 1986

This paper describes a standard cell placement strategy which consists of three kconsecutive steps` initial placement, iterative placement improvement, and string placement. In the initial placement step, cell placement was done by solving the linear ordering problem for a one-dimensional layout of standard cells and then zigzaging the resultant linear order width in the chip plane. The iterative placement improvement step is based on the iterative pairwise interchange using the estimated total routing length as a figure-or -merit. The string placement is used to reorder cells and terminals in each etandard cell row such that channel routing in the adjacent channels is not blocked by cyclic constraints and needs fewer routing tracks. The placement program is coded in PASCAL and kimplemented on a VAX-11/750 computer. Experimental results for several examples are included.