• Microbiology and Biotechnology Letters
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• v.49 no.2
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• pp.210-216
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• 2021

This study aimed to enhance ethanol productivity of Saccharomyces cerevisiae through genome editing using CRISPR/CAS9. To increase ethanol productivity, ACC1, ELO1, and OLE1 were overexpressed in S. cerevisiae using the CRISPR/CAS9 system. The strains overexpressing ACC1, ELO1, and OLE1 survived up to 24 h in YPD medium supplemented with 18% ethanol. Moreover, the ethanol yields in strains overexpressing ACC1 (428.18 mg ethanol/g glucose), ELO1 (416.15 mg ethanol/g glucose), and OLE1 (430.55 mg ethanol/g glucose) were higher than those in the control strains (400.26 mg ethanol/g glucose). In conclusion, the overexpression of these genes increased the viability of S. cerevisiae at high ethanol concentrations and the ethanol productivity without suppressing glucose consumption.

• Journal of Life Science
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• v.30 no.8
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• pp.701-707
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• 2020

Gene editing technology using the clustered regularly interspaced short palindromic repeat (CRISPR) and the CRISPR associated protein (Cas)9 has been highly anticipated in developing breeding techniques. In this study, we discuss gene scissors as a tool for silkworm molecular breeding through analysis of Bombyx mori Kynurenine 3-Monooxygenase (BmKMO) gene editing using the CRISPR/Cas9 system and analysis of generational transmission through mutagenesis and selective crossing. The nucleotide sequence of the BmKMO gene was analyzed, and three guide RNAs (gRNAs) were prepared. Each synthesized gRNA was combined with Cas9 protein and then analyzed by T7 endonuclease I after introduction into the BM-N silkworm cell line. To edit the silkworm gene, K1P gRNA and Cas9 complexes were subsequently microinjected into the silkworm embryos; the hatching rate was 18% and the incidence of mutation was 60%. The gene mutation was verified in the heterozygous G0 generation, but no phenotypic change was observed. In homozygotes generated by self-crossing, a mutant phenotype was observed. These results suggest that silkworm molecular breeding using the CRISPR/Cas9 system is possible and could be an effective way of shortening the time required.

• Journal of Educational Research in Mathematics
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• v.20 no.2
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• pp.185-202
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• 2010

This study was designed to investigate the possibility that the optimization problem solving activities based on the instrumented CAS can have an influence on the sequence of mathematics curriculum in secondary mathematics education. Some optimization problem solving activities based on CAS were constructed and executed to eleventh grade(the penultimate year of Korean high school) 7 students for nine class hours. They have experienced using CAS in mathematics class for three months, but never learned calculus. The data which consists of classroom observations(audio and video taped) and post-unit interviews with students were analyzed. In the analysis, with CAS, students can highly deal with the applied optimization problems made up of calculus, cubic equation, solution of radical equation, and graph analysis which never learned. This result shows CAS may have an influence on the sequence of mathematics curriculum in secondary mathematics education.

• Proceedings of the KAIS Fall Conference
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• 2007.05a
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• pp.112-115
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• 2007

Currently an increase in domestic sewage and industrial wastewater causes serious water pollution in Korea. To solve water pollution problems, conventional activated sludge (CAS) system is generally used in wastewater treatment plant but this process is so ineffective in nitrogen and phosphorus. Even if CAS system is the major process, it must be improved instantly so as to remove nitrogen and phosphorus. Otherwise, the serious water pollution problems can't be resolved with CAS system. Therefore this study focused on the comparison of the treatment characteristics between ASA system and CAS system. And also the mass balance of each process of ASA system. The results from operating advanced step aeration (ASA) system indicated that the removal efficiency of BOD, COD, and SS was 89.9%, 74.5%, and 89.0% respectively. In comparison, the removal efficiency of BOD, COD, and SS for CAS system was 89.5%, 71.8%, and 89.5% respectively. In addition to the results, the TN removal efficiency of ASA system was 76.5% comparing to 32.7% of CAS system. It was concluded that the TN removal efficiency of ASA system was 44% higher than CAS system. And the TP removal efficiency was 81.4% in ASA system comparing to 25.2% in CAS system. It also means that over 56% of TP was removed in ASA system comparing to CAS system.

• Journal of Periodontal and Implant Science
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• v.43 no.6
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• pp.308-314
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• 2013

Purpose: The purposes of this study were to assess the dentists' subjective satisfaction with the crestal approach sinus (CAS) kit, a device for maxillary sinus membrane elevation by the crestal approach using a special drilling system and hydraulic pressure, and to summarize the subjective satisfaction of dental implants placed after a sinus lift procedure with the CAS kit. Methods: Thirty dental clinicians who had experience with dental implant placement after a sinus lift procedure with the CAS kit from June 2010 to May 2012 were included in this study. The questionnaire for the evaluation of the dentists' subjective satisfaction with the CAS kit was sent to the respondents and returned. The questionnaire was composed of two main parts. The first part was related to the sinus membrane perforation rate. The second part was related to the dentists' subjective satisfaction with the CAS kit. Results: A total of 28 dentists answered the questionnaire. Among 924 implant cases, sinus membrane perforation occurred in 38 cases (4.1%). Among the 28 dentists, 26 dentists (92.9%) were satisfied or very satisfied with the CAS kit. In particular, 24 dentists (85.7%) reported that safety, cutting performance, and user-friendliness of the CAS drill were advantages of the CAS kit. However, 7 dentists (25%) did not routinely use the hydraulic lifter for sinus membrane elevation. Conclusions: From the survey, it was shown that the respondents were generally satisfied with the CAS kit and that the cutting performance and safety of the drill component were considered strengths of the CAS kit.

• Phonetics and Speech Sciences
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• v.6 no.3
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• pp.33-40
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• 2014

The purpose of the study was to examine alternating motion rate and its variability in children with childhood apraxia of speech (CAS) compared to typically developing children. Six children with CAS aged 9-12 years old and 10 children who were age-matched participated in the study. This study measured tokens per second and variabilities of the rates during the production of /$p^*$ a/, /$t^*$ a/, and /$k^*$ a/. For variability measures of the rates, each participant was asked to repeat speech tasks three times and the average value of the rates and its standard deviation were obtained. The results revealed that the CAS group showed slower rate only at /$k^*$ a/ than the control group. The CAS group exhibited greater variability of AMR at all the tasks than the control group. The results suggested that variability of AMR might be a more distinctive speech feature to children with CAS than the rate of the speech task.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.9
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• pp.1279-1284
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• 2011

Gelatinization properties of crude starches from acorns harvested in various countries (domestic (KAS), Chinese (CAS), and North Korea (NAS)) were analyzed, especially blue value, amylose contents, amylogram, particle size, and SEM (scanning electron microscope). Amylose contents of NAS, KAS, and CAS were 33.65%, 32.00%, and 30.48%, respectively, and a similar tendency was observed in blue value. According to amylogram, initial paste temperature was $72^{\circ}C$ for NAS and CAS and $71^{\circ}C$ for KAS, whereas peak viscosity was 580 BU for KAS and NAS and 570 BU for CAS. The cooling viscosity and setback of NAS and CAS were higher than those of KAS. The average particle size was 23.17 microns for KAS, 23.53 microns for CAS, and 82.14 microns for NAS; particle size ranging from 5~40 microns was highly distributed in KAS (68.9%) and CAS (73.8%), whereas 57.1% distribution was observed for 5~40 microns and 27.2% for >80 microns in NAS. Gelatinization ranges of SEM photography of CAS and NAS showed a more swollen, extremely disintegrated, and folded structure than that of KAS. From these results, gelatinization properties, especially viscosity, setback of amylogram, particle size, and SEM photography, differed according to the country.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.29 no.1
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• pp.43-49
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• 2023

This study reports on the preparation of sodium caseinate-cardanol (CasNa/CL)-based papers coated with different amounts of bentonite (BN) for use as a sustainable packaging material. Their chemical and morphological structures, mechanical properties, water vapor permeability, surface properties, and antioxidant activity of coated papers was assessed as a function of the BN content. The drying of the CasNa/CL coated papers led to the formation of pinholes on their surfaces owing to the presence of trapped water resulting from the difference in the drying rate between the external surface and the inside of the coated layers. Increasing the BN content reduced the pinholes on surface of CasNa/CL/BN coated papers and highly decreased the water vapor transmittance rate of the papers from 387.3±1.9 g/m²·day to 269.25±4.5 g/m²·day. Free radical scavenging assays indicated the addition of CL to the CasNa exhibited the antioxidant activity and antioxidant activity of CasNa/CL/BN did not changed as increase of BN contents. The improved water vapor barrier property and antioxidant activity of CasNa/CL/BN coated papers can be promised for various packaging applications.

• BMB Reports
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• v.50 no.5
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• pp.247-256
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• 2017

CRISPR/Cas9 is the latest tool introduced in the field of genome engineering and is so far the best genome-editing tool as compared to its precedents such as, meganucleases, zinc finger nucleases (ZFNs) and transcription activator-like effectors (TALENs). The simple design and assembly of the CRISPR/Cas9 system makes genome editing easy to perform as it uses small guide RNAs that correspond to their DNA targets for high efficiency editing. This has helped open the doors for multiplexible genome targeting in many species that were intractable using old genetic perturbation techniques. Currently, The CRISPR system is revolutionizing the way biological researches are conducted and paves a bright future not only in research but also in medicine and biotechnology. In this review, we evaluated the history, types and structure, the mechanism of action of CRISPR/Cas System. In particular, we focused on the application of this powerful tool in autophagy research.

• Journal of Korean Society of Environmental Engineers
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• v.39 no.4
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• pp.201-207
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• 2017

This study was conducted to investigate the characteristics of the stream current detector (SCD) and charge analyzing system (CAS), which are well known as the automatic coagulant dosage determination instruments. When the SCD system was used, the current charge value set at pH 9.3 could not be used as the coagulant dosage when the pH of the raw water decreased to 5.7, and the current charge value corresponding to pH 5.7 was required to be reset. In case of CAS system, polydadmac is stable as a cationic titrant when the pH of the raw water ranged 9.3 to 6.0. And it was effective to use polydadmac as a cationic titrant for high turbidity water. The current charge of polydadmac was almost constant between pH 5 and 11, however, the difference in charge of alum is six times in this pH range. Therefore, when the pH of the raw water was changed, it seems that the coagulant used in the actual water treatment plant was as a good cationic titrant.