• Title/Summary/Keyword: Capacity of use

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Production of Aminolevulinic Acid by Recombinant Escherichia coli Co-expressing hemA and otsBA Using Crude Glycerol as Carbon Source (폐글리세롤을 탄소원으로 hemA와 otsBA를 공동 발현하는 재조합대장균 배양을 통한 아미노레블린산 생산)

  • Yan, Jingmei;Pham, Diep Ngoc;Kang, Dae-Kyung;Kim, Sung Bae;Kim, Chang-Joon
    • Microbiology and Biotechnology Letters
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    • v.44 no.3
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    • pp.341-348
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    • 2016
  • This study aimed to develop a microbial process for producing aminolevulinic acid (ALA) using crude glycerol. In the culture of ALA-producing cells (Escherichia coli/pH-hemA) in a medium containing crude glycerol, the cell density and production were 1.8-fold and 1.2-fold lower than those obtained from pure glycerol, respectively. However, the cell growth and production were improved by supplementing the medium with trehalose (30 or 100 g/l). Engineered cells (E. coli/pH-hemA/pS-otsBA) were constructed to express otsBA and their culture performance was compared with that of control cells (E. coli/pH-hemA/ pSTV28). The effects of isopropyl β-D-1-thiogalactopyranoside (IPTG) concentration and the time of induction were examined to improve the cell growth and ALA production in engineered cells cultured using crude glycerol. When 0.6 mM of IPTG was added at the beginning of the exponential growth phase, the ALA produced by cells was 2,121 mg/l, which was comparable to that from pure glycerol. The results demonstrate that otsBA expression endowed cells with the capacity to tolerate the toxicity of crude glycerol for direct use.

An experimental study on bonding and bearing capacities of thin spray-on liner to evaluate its applicability as a tunnel support member (터널 지보재로서의 적용성 검토를 위한 박층 뿜칠 라이너의 부착성능과 지보성능의 평가)

  • Han, Jin-Tae;Lee, Gyu-Phil;Park, Young-Taek;Choi, Soon-Wook;Hwang, Gwi-Sung;Choi, Myung-Sik;Chang, Soo-Ho
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.15 no.6
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    • pp.571-583
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    • 2013
  • The use of Thin Spray-on Liner (TSL) as an alternative to shotcrete has drastically increased since 1990s when it was first developed and introduced to mines. In this study, tensile strength test, bond strength test, compression test with specimens coated by TSL, and two kinds of bending tests proposed by EFNARC (2008) were performed with two kinds of TSLs with different material compositions in order to evaluate their support capacities. As a result, both TSLs were shown to be satisfactory for the minimum performance requirements for a structural rock support suggested by EFNARC (2008) and tensile strength of a TSL was shown to increase as its content of polymer was higher. In contrast, its bond strength was shown to increase proportional to the content of a cementitious component especially at the early age.

A Study on the Hydraulic Properties of Aquifers in Bucheon Area (부천지역의 지하수 대수층의 수리적 특성에 관한 연구)

  • Kim, So-Hee;Lee, Yoon-Jin
    • Journal of Korean Society of Environmental Engineers
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    • v.28 no.6
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    • pp.626-633
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    • 2006
  • Bucheon city has been rapidly urbanized and steadily enlarged. Also, the city depends on groundwater as an alternative water resource. The objective of this study is to characterize the hydrogeology in this city including the recharge of groundwater and hydraulic property of an aquifer in order to investigate the chemical characteristics and contamination of groundwater in the Bucheon area. Eleven groundwater wells in the Bucheon area were selected for applying certain hydraulic tests, such as pumping and recovery tests. Theis method and Theis recovery method were used to obtain hydraulic parameters, such as hydraulic conductivity, transmissivity by applying recovery tests. The playground of school and uncovered areas layed a major function of recharging areas. The groundwater well in these areas showed high recharge, transmissivity, and hydraulic conductivity. In a downtown area, groundwaier wells showed low pumping capacity and hydraulic parameters. Groundwater samples were collected from 48 different locations in the Bucheon area and that showed very various chemical composition in groundwater Their electrical conductivity showed various ranges from 70.4 to $1,287{\mu}S/cm$. The composition more significantly affected the groundwater due to the land use and urbanization than that of the aquifer rock type.

A Study on Hybrid DeNOx Process Using Selective Catalytic Reduction and Adsorption (선택적촉매환원과 흡착을 이용한 복합 탈질공정 연구)

  • Moon, Seung-Hyun;Jeon, Dong-Hwan;Park, Sung-Youl
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.12
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    • pp.1329-1336
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    • 2007
  • This study was carried out to develop an efficient process abating high NO concentration. A hybrid process of selective catalytic reduction(SCR) and activated carbon fiber(ACF) adsorption was newly designed and tested. Used ACF in NO adsorption was regenerated by simultaneously applying heat and vacuum. The result of ACF regeneration was for superior in the desorption condition at $140^{\circ}C$ and vacuum 600 mmHg. A commercial catalyst was used at the conditions of reaction temperature at $300^{\circ}C$, $NH_3/NO$ mole ratio = 1.0 for SCR process. NO evolved from ACF regeneration reactor could be removed by SCR reactor up to 98%. But high concentration of NO was exhausted from SCR reactor for one minute when the flue gas of NO 300 ppm and deserted NO from ACF regeneration were simultaneously treated by the same SCR reactor. Therefore, it is necessary to use additional small sized SCR reactor or to increase $NH_3$ concentration for a short time along with NO concentration rather than to mix flue gas with the gas evolving from ACF regeneration at fixed $NH_3$ inlet concentration. The hybrid process of SCR and ACF showed high NO removal efficiency over 80% at any time courses. Through the repeated cycles, stable DeNOx efficiency was maintained, indicating that the hybrid process would be a good countermeasure to the spotaneously high NO concentration instead of increasing the SCR capacity.

Estimating Travel Demand by Using a Spatial-Temporal Activity Presence-Based Approach (시.공간 활동인구 추정에 의한 통행수요 예측)

  • Eom, Jin-Ki
    • Journal of Korean Society of Transportation
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    • v.26 no.5
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    • pp.163-174
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    • 2008
  • The conventional four-step travel demand model is still widely used as the state-of-practice in most transportation planning agencies even though it does not provide reliable estimates of travel demand. In order to improve the accuracy of travel demand estimation, implementing an alternative approach would be critical as much as acquiring reliable socioeconomic and travel data. Recently, the role of travel demand model is diverse to satisfy the needs of microscopic analysis regarding various policies of travel demand management and traffic operations. In this context, the activity-based approach for travel demand estimation is introduced and a case study of developing a spatial-temporal activity presence-based approach that estimates travel demand through forecasting number of people present at certain place and time is accomplished. Results show that the spatial-temporal activity presence-based approach provides reliable estimates of both number of people present and trips actually people made. It is expected that the proposed approach will provide better estimates and be used in not only long-term transport plans but short-term transport impact studies with respect to various transport policies. Finally, in order to introduce the spatial-temporal activity presence-based approach, the data such as activity-based travel diary and land use based on geographic information system are essential.

A Simple Seismic Vulnerability Sorting Method for Electric Power Utility Tunnels (전력구의 간편 지진취약도 선별법)

  • Kang, Choonghyun;Huh, Jungwon;Park, Inn-Joon;Hwang, Kyeong Min;Jang, Jung Bum
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.22 no.5
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    • pp.110-118
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    • 2018
  • Due to recent earthquakes, there is a growing awareness that Korea is not a safe zone for earthquakes any more. Therefore, the review of various aspects of the seismic safety of the infrastructures are being carried out. Because of the characteristics of the underground structure buried in the ground, the electric power utility tunnels must be considered not only for the inertia and load capacity of the structure itself but also the characteristics of the surrounding soils. An extensive and accurate numerical analysis is inevitably required in order to consider the interaction with the ground, but it is difficult to apply the soil-structure interaction analyses, which generally requires high cost and extensive time, to all electric power utility tunnel structures. In this study, the major design variables including soil characteristics are considered as independent variables, and the seismic safety factor, which is the result of the numerical analysis, is considered as a dependent variable. Thus, a method is proposed to select vulnerable electric power utility tunnels with low seismic safety factor while excluding costly and time-consuming numerical analyses through the direct correlation analysis between independent and dependent variables. Equations of boundary limits were derived based on the distribution of the seismic safety factor and the cover depth and rebar amounts with high correlation relationship. Consequently, a very efficient and simple approach is proposed to select vulnerable electric power utility tunnels without intensive numerical analyses. Among the 108 electric power utility tunnels that were investigated in this paper, 30% were screened as fragile structures, and it is confirmed that the screening method is valid by checking the safety factors of the fragile structure. The approach is relatively very simple to use and easy to expand, and can be conveniently applied to additional data to be obtained in the future.

Application of Alkali-Activated Ternary Blended Cement in Manufacture of Ready-Mixed Concrete (알칼리 활성화 3성분계 혼합시멘트의 레미콘 적용 시험)

  • Yang, Wan-Hee;Hwang, Ji-Soon;Lee, Sea-Hyun
    • Journal of the Korea Institute of Building Construction
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    • v.17 no.1
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    • pp.47-54
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    • 2017
  • Cement industry is typical carbon-emission industry. If the industrial by-products(granulated blast-furnace slag (GGBFS), fly ash, etc.) are used a large amount, it might be able to reduce cement consumption and mitigate carbon emissions. In this case, however, decrease of early strength is relatively large. Therefore, there is a limitation in increase of the amount of substitute. Considering these circumstances, it would be a good solution to reduce carbon emissions in cement industry to improve the performances of mixed cement through proper alkali-activation in Portland blended cement using GGBFS or fly ash. Therefore, this study prepared concrete in ready-mixed concrete manufacturing facilities with an addition of a binder which used 2.0% modified alkali sulfate activator after mixing Portland cement, GGBFS and fly ash in the ratio of 4:4:2 and assessed its basic properties. The results found the followings: The use of modified alkali-sulfate activator slightly reduced slump and shortened setting time. As a result, bleeding capacity decreased while early strength improved. In addition, there is no big difference in carbonation resistance. It appears that there should be continued experiments and analyses on the related long-term aged specimens.

K-means clustering analysis and differential protection policy according to 3D NAND flash memory error rate to improve SSD reliability

  • Son, Seung-Woo;Kim, Jae-Ho
    • Journal of the Korea Society of Computer and Information
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    • v.26 no.11
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    • pp.1-9
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    • 2021
  • 3D-NAND flash memory provides high capacity per unit area by stacking 2D-NAND cells having a planar structure. However, due to the nature of the lamination process, there is a problem that the frequency of error occurrence may vary depending on each layer or physical cell location. This phenomenon becomes more pronounced as the number of write/erase(P/E) operations of the flash memory increases. Most flash-based storage devices such as SSDs use ECC for error correction. Since this method provides a fixed strength of data protection for all flash memory pages, it has limitations in 3D NAND flash memory, where the error rate varies depending on the physical location. Therefore, in this paper, pages and layers with different error rates are classified into clusters through the K-means machine learning algorithm, and differentiated data protection strength is applied to each cluster. We classify pages and layers based on the number of errors measured after endurance test, where the error rate varies significantly for each page and layer, and add parity data to stripes for areas vulnerable to errors to provides differentiate data protection strength. We show the possibility that this differentiated data protection policy can contribute to the improvement of reliability and lifespan of 3D NAND flash memory compared to the protection techniques using RAID-like or ECC alone.

Effect of Phase Change Material on Hydration Heat of Mortar with Fly Ash and Blast Furnace Slag (상전이물질이 플라이애시 및 고로슬래그를 혼입한 모르타르의 수화발열에 미치는 영향)

  • Nam, Yi-Hyun;Jang, Seok-Joon;Kim, Sun-Woong;Park, Wan-Shin;Yun, Hyun-Do
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.23 no.1
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    • pp.1-8
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    • 2019
  • Phase change material(PCM) has the capacity to absorb or release energy in heat when the phase changes. This study conducted to investigate the effect of strontium-based PCM on the hydration heat and mechanical properties of mortar with fly ash and blast furnace slag. The amounts of PCM were 1%, 2%, 3%, 4%, and 5% by the cementitious materials weight. The tests about mortar flow, semi-adiabatic temperature rise, compressive and flexural strength tests were carried out for twelve types of mortar mixtures. The test results indicated that the use of PCM was effective to reduce hydration heat and retard hydration of mortar with industrial by-products. In particular, the heat generation rate of mortars with fly ash was lower than that of mortars with blast furnace slag. The compressive strength of mortar with fly ash and blast furnace slag were decreased with increasing PCM ratio.

Prediction of Long-term Behavior of Ground Anchor Based on the Field Monitoring Load Data Analysis (현장 하중계 계측자료 분석을 통한 그라운드 앵커의 장기거동 예측)

  • Park, Seong-yeol;Hwang, Bumsik;Lee, Sangrae;Cho, Wanjei
    • Journal of the Korean Geotechnical Society
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    • v.37 no.8
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    • pp.25-35
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    • 2021
  • Recently, the ground anchor method is commonly applied with nail and rock bolt to secure the stability of slopes and structures in Korea. Among them, permanent anchor which is used for long-term stability should secure bearing capacity and durability during the period of use. However, according to recent studies, phenomenon such as deformation to slope and the reduction of residual tensile load over time have been reported along the long-term behavior of the anchors. These problems of reducing residual tensile load are expected to increase in the future, which will inevitably lead to problems such as increasing maintenance costs. In this study, we identified the factors that affect the tensile load of permanent anchor from a literature study on the domestic and foreign, and investigated the prior studies that analyzed previously conducted load cell monitoring data. Afterwards, using this as basic data, the load cell measurement data collected at the actual site were analyzed to identify the tensile load reduction status of anchors, and the long-term load reduction characteristics were analyzed. Finally, by aggregating the preceding results, proposed a technique to predict the long-term load reduction characteristics of permanent anchors through short-term data to around 100 days after installation.