• Journal of Microbiology and Biotechnology
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• 제30권9호
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• pp.1282-1289
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• 2020

Previously, we performed an in silico analysis of the Periplaneta americana transcriptome. Antimicrobial peptide candidates were selected using an in silico antimicrobial peptide prediction method. It was found that periplanetasin-5 had antimicrobial activity against yeast and gram-positive and gram-negative bacteria. In the present study, we demonstrated the anti-inflammatory activities of periplanetasin-5 in mouse macrophage Raw264.7 cells. No cytotoxicity was observed at 60 ㎍/ml periplanetasin-5, and treatment decreased nitric oxide production in Raw264.7 cells exposed to lipopolysaccharide (LPS). In addition, quantitative RT-PCR and enzyme-linked immunosorbent assay revealed that periplanetasin-5 reduced cytokine (tumor necrosis factor-α, interleukin-6) expression levels in the Raw264.7 cells. Periplanetasin-5 controlled inflammation by inhibiting phosphorylation of MAPKs, an inflammatory signaling element, and reducing the degradation of IκB. Through LAL assay, LPS toxicity was found to decrease in a periplanetasin-5 dose-dependent manner. Collectively, these data showed that periplanetasin-5 had anti-inflammatory activities, exemplified in LPS-exposed Raw264.7 cells. Thus, we have provided a potentially useful antibacterial peptide candidate with anti-inflammatory activities.

• Nutrition Research and Practice
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• 제12권2호
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• pp.110-117
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• 2018

BACKGROUND/OBJECTIVES: Non-alcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver disease and is closely associated with metabolic syndrome. In the present study, we observed the effect of ethanol extract of Allium fistulosum (EAF) on NAFLD and have suggested the possibility of using EAF as a natural product for application in the development of a treatment for NAFLD. MATERIALS/METHODS: The preventive effect on hepatic lipid accumulation was estimated by using an oleic acid (OA)-induced NAFLD model in vitro and a Western diet (high-fat high-sucrose; WD)-induced obese mouse model. Animals were divided into three groups (n = 7): normal diet group (ND), WD group, and WD plus 1% EAF group. RESULTS: EAF reduced OA-stimulated lipid accumulation in HepG2 cells in the absence of cellular cytotoxicity and significantly blocked transcriptional activation of sterol regulatory element-binding protein 1 and fatty acid synthase genes. Subsequently, we investigated these effects in vivo in mice fed either ND or WD in the presence or absence of EAF supplementation. In comparison to the ND controls, the WD-fed mice exhibited increases in body weight, liver weight, epididymal fat weight, and accumulation of fat in hepatocytes, and these effects were significantly attenuated by EAF supplementation. CONCLUSIONS: Allium fistulosum attenuates the development of NAFLD, and EAF elicits anti-lipogenic activity in liver. Therefore, EAF represents a promising candidate for use in the development of novel therapeutic drugs or drug combinations for the prevention and treatment of NAFLD.

• 한국수자원학회논문집
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• 제32권2호
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• pp.99-110
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• 1999

모멘트법은 Lagrangian 벙법으로서 격자요소 내에서의 농도의 공간분포에 대한 0차, 1차, 2차 모멘트를 고려한고 각 모멘트의 보존성을 유지하면서 농도분포의 이송을 계산하는 방법이다. 따라 각 격자요소에서의 0차 모멘트, 즉 평균농도 뿐만 아니라 1차 및 2차 모멘트 값의 합리적인 초기 설정이 요구된다. 본 연구에서는 각 모멘트들의 초기값 설정방법을 검토하고, 기존 모멘트법의 Couuant 수에 대한 제약조건을 극복하기 위하여 모멘트법을 개선하였다. 모멘트법에 의한 모의 결과를 유용한 Eulerian 및 Lagrangian 기법에 의한 모의 결과와 비교 검토하여 모한 해석결과를 발생시키는 기법이며, 본 연구에서 제시한 Courant 수 제약조건의 극복에 관한 연구는 성공적으로 이루어진 것으로 나타났다. 한편, 모멘트법은 농도가 전체 계산영역의 일부에 분포하는 2차원 영역에서의 이송 모의시 계산시간에 있어서 매우 효율적인 것으로 나타났다.

• 대한화장품학회지
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• 제44권4호
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• pp.375-379
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• 2018

본 연구진은 메틸말리올라이드가 인간 피부 세포주인 HaCaT 세포에서 NRF2를 유도하고 이를 통하여 항산화 효과를 나타내는지 알아보았다. MTT assay를 통하여 메틸말리올라이드는 $10{\mu}M$ 농도에서 24 h 동안 HaCaT 세포에 처리하여도 HaCaT 세포 독성을 나타내지 않는 것을 확인하였다. 본 연구진이 구축한 HaCaT-ARE-GFP-luciferase 세포에 메틸말리올라이드를 처리하고 루시퍼라아제 활성을 측정한 결과 메틸말리올라이드는 양성 대조군인 레스베라트롤보다 ARE 결합에 따른 루시퍼라아제 활성을 더욱 강하게 증가시키는 것을 확인하였다. 또한 HaCaT 세포에 메틸말리올라이드 처리 시 NRF2에 의하여 유도되는 항산화 단백질인 HO-1과 NQO1 mRNA 및 단백질 발현이 통계적으로 유의하게 증가하였다. 마지막으로 메틸말리올라이드는 HaCaT 세포에서 TPA에 의해서 유도된 DNA 및 지질의 산화를 강력하게 억제하였다. 결론적으로 본 연구는 메틸말리올라이드가 NRF2 유도를 통하여 피부 산화적 스트레스를 억제하며 이는 메틸말리올라이드가 신규 항산화 화장품의 소재로 적합하다는 것을 시사한다.

• Journal of Ginseng Research
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• 제43권3호
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• pp.488-495
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• 2019

Background: Timosaponin AIII (TA3) is a steroidal saponin extracted from Anemarrhena asphodeloides. Here, we investigated the anticancer effects of TA3 in MG63 human osteosarcoma cells. TA3 attenuates migration and invasion of MG63 cells via regulations of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, which are involved with cancer metastasis in various cancer cells. TA3 reduced enzymatic activities and transcriptional expressions of MMP-2 and MMP-9 in MG63 cells. TA3 also inhibited Src, focal adhesion kinase, extracellular signal-regulated kinase (ERK1/2), c-Jun N-terminal kinase (JNK), p38, ${\beta}-catenin$, and cAMP response element binding signaling, which regulate migration and invasion of cells. TA3 induced apoptosis of MG63 cells via regulations of caspase-3, caspase-7, and poly(ADP-ribose) polymerase (PARP). Then, we tested several ginsenosides to be used in combination with TA3 for the synergistic anticancer effects. We found that ginsenosides Rb1 and Rc have synergistic effects on TA3-induced apoptosis in MG63 cells. Methods: We investigated the anticancer effects of TA3 and synergistic effects of various ginseng saponins on TA3-induced apoptosis in MG63 cells. To test antimetastatic effects, we performed wound healing migration assay, Boyden chamber invasion assays, gelatin zymography assay, and Western blot analysis. Annexin V/PI staining apoptosis assay was performed to determine the apoptotic effect of TA3 and ginsenosides. Results: TA3 attenuated migration and invasion of MG63 cells and induced apoptosis of MG63 cells. Ginsenosides Rb1 and Rc showed the synergistic effects on TA3-induced apoptosis in MG63 cells. Conclusions: The results strongly suggest that the combination of TA3 and the two ginsenosides Rb1 and Rc may be a strong candidate for the effective antiosteosarcoma agent.

• Journal of Information Science Theory and Practice
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• 제9권2호
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• pp.1-17
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• 2021

Pseudo relevance feedback (PRF) is a powerful query expansion (QE) technique that prepares queries using the top k pseudorelevant documents and choosing expansion elements. Traditional PRF frameworks have robustly handled vocabulary mismatch corresponding to user queries and pertinent documents; nevertheless, expansion elements are chosen, disregarding similarity to the original query's elements. Word embedding (WE) schemes comprise techniques of significant interest concerning QE, that falls within the information retrieval domain. Deep averaging networks (DANs) defines a framework relying on average word presence passed through multiple linear layers. The complete query is understandably represented using the average vector comprising the query terms. The vector may be employed for determining expansion elements pertinent to the entire query. In this study, we suggest a DANs-based technique that augments PRF frameworks by integrating WE similarities to facilitate Arabic information retrieval. The technique is based on the fundamental that the top pseudo-relevant document set is assessed to determine candidate element distribution and select expansion terms appropriately, considering their similarity to the average vector representing the initial query elements. The Word2Vec model is selected for executing the experiments on a standard Arabic TREC 2001/2002 set. The majority of the evaluations indicate that the PRF implementation in the present study offers a significant performance improvement compared to that of the baseline PRF frameworks.

• 한국정보통신학회논문지
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• 제23권10호
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• pp.1269-1274
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• 2019

본 논문에서는 5G 어플리케이션을 위한 네 개의 마이크로스트립 패치 배열 안테나를 설계 및 분석하였다. 제안한 배열 안테나는 네 개의 사각형 마이크로스트립 패치 안테나로 구성되며, 배열 안테나의 양쪽 측면에 반원형 에칭구조가 포함된다. 안테나는 시리즈 및 동일 급전 네트워크를 사용하여 공급되며, 하단의 접지면은 안테나의 주파수 특성을 개선하기 위해 변경된다. 마지막으로 안테나의 지향성 특성을 향상시키기 위해 야기-형 구조가 추가된다. 제안한 마이크로스트립 패치 배열 안테나는 21.95 ~ 31.86 GHz의 넓은 주파수 대역폭을 확보하였다. 안테나의 이득은 28 GHz대역에서 9.7 dBi의 이득을 보였으며, 제안한 주파수 대역 전반에 걸쳐 높은 이득과 높은 지향성을 유지하였다. 제안한 안테나는 낮은 프로파일과 간단한 구조로 인해 5G 어플리케이션을 위한 좋은 대안될 수 있을 것이다.

• 한국방사선학회논문지
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• 제16권1호
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• pp.53-59
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• 2022

CT영상을 기반으로 한 QCT는 CT장비와 컴퓨터영상처리기술의 발전과 함께 적은 방사선 투과양으로도 체적골밀도를 측정할 수 있는 방향으로 크게 발전하고 있다. 방사선 의료영상을 대표적인 골밀도 측정법인 이중방사선흡수계수법(DXA)은 척추나 근위대퇴골과 다른 뼈와의 상관성이 떨어지는 경우가 많고질량효과에 의한 부정확성의 근본 원인을 가지고 있기 때문에 체적골밀도측정법인 단일에너지CT영상을 이용한 QCT법이 가장 보편적인 정확한 골밀도 측정법이다. 이중에너지CT의 사용은 단단한 뼈와 동시에 종양이나 연부조직의 이상을 같이 볼 수 있는 영상을 얻을 수 있게 되어 QCT 골밀도와 함께 특정연부조직이나 장기를 검사할 수 있어 그 사용이 더욱 늘어날 것으로 예상된다. 임상수술이나 추적검사가 필요한 위치에 사용자가 해부학적으로 특정한 형상체적에 대한 국부골밀도측정 방법도 병원의 임상현장에서 활용할 기술로 대두되고 있다.

• Structural Engineering and Mechanics
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• 제83권3호
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• pp.293-304
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• 2022

In this work, a novel reliability approach for combined high and low cycle fatigue (CCF) estimation is developed by combining active learning strategy with least squares support vector machines (LS-SVM) (named as ALS-SVM) surrogate model to address the multi-resources uncertainties, including working loads, material properties and model itself. Initially, a new active learner function combining LS-SVM approach with Monte Carlo simulation (MCS) is presented to improve computational efficiency with fewer calls to the performance function. To consider the uncertainty of surrogate model at candidate sample points, the learning function employs k-fold cross validation method and introduces the predicted variance to sequentially select sampling. Following that, low cycle fatigue (LCF) loads and high cycle fatigue (HCF) loads are firstly estimated based on the training samples extracted from finite element (FE) simulations, and their simulated responses together with the sample points of model parameters in Coffin-Manson formula are selected as the MC samples to establish ALS-SVM model. In this analysis, the MC samples are substituted to predict the CCF reliability of turbine blades by using the built ALS-SVM model. Through the comparison of the two approaches, it is indicated that the reliability model by linear cumulative damage rule provides a non-conservative result compared with that by the proposed one. In addition, the results demonstrate that ALS-SVM is an effective analysis method holding high computational efficiency with small training samples to gain accurate fatigue reliability.

• Nuclear Engineering and Technology
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• 제53권7호
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• pp.2174-2183
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• 2021

Helium cooled solid breeder blanket as an important blanket candidate of the Tokamak fusion reactor uses ceramic pebble bed for tritium breeding. Considering the poor effective thermal conductivity of the ceramic breeder pebble bed, thin structure of tritium breeder pebble bed is usually adopted in the blanket design. The container wall has a great influence on the thin pebble bed packing structure, especially for the assembly of mono-sized particles, and thin pebble bed will appear anisotropic effective thermal conductivity phenomenon. In this paper, thin ceramic pebble beds composed of 1 mm diameter Li₄SiO₄ particles are generated by the EDEM 2.7. The effective thermal conductivity of different thickness pebble beds in the three-dimensional directions are analyzed by three-dimensional thermal network method. It is observed that thin Li₄SiO₄ pebble bed showing anisotropic effective thermal conductivity under the practical design size. Normally, the effective thermal conductivity along the bed vertical direction is higher than the horizontal direction due to the gravity effect. As the thickness increases from 10 mm to 40 mm, the effective thermal conductivity of the pebble bed gradually increases.