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http://dx.doi.org/10.4014/jmb.2004.04046

Anti-Inflammatory Activity of Antimicrobial Peptide Periplanetasin-5 Derived from the Cockroach Periplaneta americana  

Kim, In-Woo (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Joon Ha (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Seo, Minchul (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Lee, Hwa Jeong (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Baek, Minhee (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Mi-Ae (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Shin, Yong Pyo (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Sung Hyun (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Iksoo (College of Agriculture and Life Sciences, Chonnam National University)
Hwang, Jae Sam (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
Publication Information
Journal of Microbiology and Biotechnology / v.30, no.9, 2020 , pp. 1282-1289 More about this Journal
Abstract
Previously, we performed an in silico analysis of the Periplaneta americana transcriptome. Antimicrobial peptide candidates were selected using an in silico antimicrobial peptide prediction method. It was found that periplanetasin-5 had antimicrobial activity against yeast and gram-positive and gram-negative bacteria. In the present study, we demonstrated the anti-inflammatory activities of periplanetasin-5 in mouse macrophage Raw264.7 cells. No cytotoxicity was observed at 60 ㎍/ml periplanetasin-5, and treatment decreased nitric oxide production in Raw264.7 cells exposed to lipopolysaccharide (LPS). In addition, quantitative RT-PCR and enzyme-linked immunosorbent assay revealed that periplanetasin-5 reduced cytokine (tumor necrosis factor-α, interleukin-6) expression levels in the Raw264.7 cells. Periplanetasin-5 controlled inflammation by inhibiting phosphorylation of MAPKs, an inflammatory signaling element, and reducing the degradation of IκB. Through LAL assay, LPS toxicity was found to decrease in a periplanetasin-5 dose-dependent manner. Collectively, these data showed that periplanetasin-5 had anti-inflammatory activities, exemplified in LPS-exposed Raw264.7 cells. Thus, we have provided a potentially useful antibacterial peptide candidate with anti-inflammatory activities.
Keywords
Periplanetasin-5; antimicrobial peptide; anti-inflammatory activity; cockroach; Periplaneta americana;
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