• Korean Journal of Medicinal Crop Science
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• v.19 no.3
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• pp.162-169
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• 2011

The rhizomes and herbal medicines originating from Acorus gramineus, A. calamus, A. tatarinowii, and A. gramineus var. pusilus, show significant similarity, and the correct identification of species is very difficult. Random Amplified Polymorphic DNA (RAPD) and Sequence Characterized Amplified Region (SCAR) were used to develop a reliable method for identification of these four species. Several distinct SCAR markers were developed from species-specific RAPD amplicons for each species. Furthermore, a useful molecular marker was established for multiplex-PCR, in order to the four species could be distinguished concurrently. These markers allow efficient and rapid identification of closely-related Acorus species and will be useful for standardization of herbal medicines.

• Korean Journal of Environment and Ecology
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• v.29 no.2
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• pp.105-131
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• 2015

This study was carried out to investigate the flora of Gyodongdo island (Ganghwa-gun). The vascular plants from 11 field surveys were revealed to belong to a total of 629 taxa; 118 families, 364 genera, 561 species, 5 subspecies, 53 varieties, 7 forms and 3 hybrids. 184 taxa were the first records from this region. The plants in Gyodongdo island are composed of the deciduous broad-leaved and conifer-mixed forests which are the common ones in the middle part of the Korean Peninsula. Five taxa of Korean endemic plants such as Clematis brachyura Maxim., Viola seoulensis Nakai, Populus ${\times}$ tomentiglandulosa T. B. Lee, Forsythia koreana (Rehder) Nakai and Hemerocallis hakuunensis Nakai were collected. Endangered wild plants designated by the law called 'Protection Law for Endangered wild fauna and flora' were one taxon. The red list plants according to IUCN valuation basis were examined for 13 taxa; endangered (EN) species of Prunus yedoensis Matsum., Vulnerable (VU) species of both Utricularia pilosa (Makino) Makino and Iris ruthenica var. nana Maxim., Near Threatened (NT) species of Senecio argunensis Turcz., Least Concern (LC) species of Platycladus orientalis (L.) Franco, Potentilla discolor Bunge, Limnophila sessiliflora (Vahl) Blume, Acorus calamus L., Phacelurus latifolius (Steud.) Ohwi, Pseudoraphis ukishiba Ohwi, Belamcanda chinensis (L.) DC., and Not Evaluate (NE) species of both Astragalus sikokianus Nakai and Potamogeton oxyphyllus Miq. The floristic regional indicator plants found in this area were a total of 47 taxa comprising three taxa of grade V, four taxa of grade IV, nine taxa of grade III, 10 taxa of grade II, and 21 taxa of grade I. The naturalized plants were identified as 62 taxa and the percentage of naturalized index (NI) was 9.9 % and the percentage of urbanization index (UI) was 19.3 %, respectively. Furthermore, hemicryptophytes (28 %), therophytes (26 %), hydrophytes (13 %) and geophyte (12 %) showed high proportional ratio in life form spectrum.

• Korean Journal of Plant Resources
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• v.28 no.4
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• pp.475-486
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• 2015

This survey was conducted in order to investigate the vascular plants of Hak Reservoir Valley in the DMZ Area, Korea. The vascular plants were collected six times (from April 2014 to November 2014) and consisted a total of 238 taxa; 69 families, 172 genera, 206 species, 2 subspecies, 26 varieties and 4 forms. The observed rare plant species as designated by Korea Forest Service were 3 taxa including Carex capricornis Meinsh. ex Maxim., Acorus calamus L., Penthorum chinense Pursh. Endemic plant species were 2 taxa including Salix koriyanagi Kimura, Clematis brachyura Maxim. 17 taxa were listed as specific plant species based on phytogeographical in the investigated area. 25 taxa were recorded as naturalized plants, and their Naturalization Ratio and Urbanization Index were recorded as 10.46%, and 7.78%, respectively. Of the 238 taxa listed; 105 were pasturing plants, 92 edible plants, 76 medicinal plants, 24 ornamental plants, 6 stain plants, 4 timber plants, 2 fiber plants, 2 industrial plants and 55 unknown plants.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.5 no.4
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• pp.31-38
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• 2002

The rate of sewage treatment in South Korea was 68% in the late 1999. Sewage treatment is mostly made near big cities such as Seoul and Busan, and it is little in rural areas. Wastewater from households goes to streams directly without treatment in rural areas and pollutes streams. It is necessary to improve the progress for treatment of nutrients such as N and P which cause eutrophication in streams and lakes, because sewage treatment system in South Korea focuses on treatment of basic organic matters. Therefore it will contribute to improve discharged water quality if small sewage treatment systems by aquatic plants and microbes are introduced to rural areas where are not connected to local sewage treatment facility. This experiment was conducted to find out the best way using aquatic plants and microbes to purify wastewater from households through individual sewage treatment system. Phragmites communis, Iris pseudoacorus, Acorus calamus var. angustatus, Typha orientalis and Oenanthe javanica were used for this experiment. BOD, COD, SS, T-N and T-P were analyzed following standard methods for wastewater. The result shows that wastewater was roughly purified through pebbles and sands, and highly purified through aquatic plants and microbes especially in T-N and T-P. Iris pseudoacorus is the most effective in reduction of COD and BOD level. This system will work even in winter when plants die because microbes will be still working.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.4
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• pp.61-68
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• 2004

The purpose of this study was to develop environmentally friendly constructed wetland system in order to improve the environment. This system was constructed with two constructed wetlands andone pond. The size of the first and second wetland was 2.5m in length, 2.5m in width and 0.7m in depth for the first wetland and 0.6m in depth for the second wetland. Those were filled with pebbles with about 16~32mm in diameter from bottom to 20cm depth and onto the pebbles with about 0.5 mm in diameter sand in depth 40cm. The first constructed Wetland was planted with pragmites communis. The second was planted with Iris pseudoacorus and Acorus calamus var. aneustatus.A vertical flow system was used in the first constructed wetland and a horizontal flow system in the second. The water of outflow from the second wetland flowed into the pond. This system was installed in Yangpyeong, Kyunggi Province. The Quality of inflow and outflow were analyzed at the first time from May 20 to May 30, 2002 and at second time from June 10 to July 18, 2002. At the second period wetland was implanted with microbes in order to improve the efficiency of constructed wetlands. Following standard methods for wastewater, BOD, COD, SS, T-N and T-P were analyzed. This system was effective in reducing COD, BOD, SS, T-N and T-P level. The result shows that wastewater was purified through constructed wetland system with plants and highly purified with microbes especially in T-P. The Average total phosphorous concentration of influent and effluent in constructed wetland with microbes was 2.8mg/${\ell}$ L and 0.21mg/${\ell}$ respectively. This system can be used in rural community because this is not only effective on purification of sewage but also is harmonized with the surrounding nature.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.2 no.1
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• pp.26-31
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• 2021

Reproduction and molt are costly processes in avian life histories. These two fitness-related traits are expected to be under one of physiological trade-offs. Age-related molt is known to be higher in young birds than that in adults presumably due to the cost of reproduction in adults. The present study partially replicated a previous study using a non-invasive method of seasonal wing feather loss instead of capture-inspection for molting progress in oriental storks (Ciconia boyciana). We first examined characteristics of the known six wing feather types (i.e., primaries [P], primary coverts [PC], secondaries [S], secondary coverts [SC], and tertials [T]) from two specimens with four wings. Results were utilized as references for further investigation. We then collected a total of 3,807 wing feathers shedded by 61 captive storks for one year and classified them into six wing feather types based on the reference with structures of vane (i.e., how asymmetrical) and calamus (i.e., how rigidly attached to skin) of wing feathers. Our results indicated that annual losses of all six-type wing feathers decreased with increasing ages, ranging from 29% to 58% for PC, alula, SC, P, S, and T in order. Our results were also comparable to those of a former study, suggesting that the pattern of age-specific molt might be associated with the cost of reproduction in adults. However, juveniles might shed more wing feathers with low quality formed during the previous development stage than older birds.

• Korean Journal of Ecology and Environment
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• v.35 no.4 s.100
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• pp.285-294
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• 2002

The purpose of this study was to evaluate the relationships between purification characteristics and hydraulic conditions, and to clarify the basic and essential factors required to be considered in the construction and management of artificial wetland system for the improvement of reservoir water quality. The artificial wetland system was composed of a pumping station and six sequential plants beds with five species of macrophytes: Oenanthe javanica, Acorus calamus, Zizania latifolia, Typha angustifolia, and Phragmites australis. The system was operated on free surface-flow system, and operation conditions were $3,444-4,156\; m^3/d$ of inflow rate, 0.5-2.0 hr of HRT, 0.1-0.2 m of water depth, 6.0-9.4 m/d of hydraulic loading, and relatively low nutrients concentration (0.224-2.462 mgN/L, 0.145-0.164 mgP/L) of inflow water. The mean purification efficiencies of TN ranged from 12.1% to 14.3% by showing the highest efficiency at the Phragmites australis bed, and these of TP were 6.3-9.5% by showing the similar ranges of efficiencies among all species. The mean purification efficiencies of SS and Chl-A ranged from 17.4% to 38.5% and from 12.0% to 20.2%, respectively, and the Oenanthe javanica bed showed the highest efficiency with higher concentration of influent than others. The mean purification amount per day of each pollutant were $9.8-4.1\;g{\cdot}m^{-2}{\cdot}d^{-1}$ in BOD, $1.299-2.343\;g{\cdot}m^{-2}{\cdot}d^{-1}$ in TN, $0.085-1.821\;g{\cdot}m^{-2}{\cdot}d^{-1}$ in TP, $17.9-111.6\;g{\cdot}m^{-2}{\cdot}d^{-1}$ in SS and $0.011-0.094\;g{\cdot}m^{-2}{\cdot}d^{-1}$ in Chl-a. The purification amount per day of TN revealed the hi링hest level at the Zizania latifolia bed, and TP showed at the Acrous calamus bed. SS and Chl-a, as particulate materials, revealed the highest purification amount per day at the Oenanthe javanica bed that was high on the whole parameters. It was estimated that the purification amount per day was increased with the high concentration of influent and shoot density of macrophytes, as was shown in the purification efficiency. Correlation coefficients between purification efficiencies and hydraulic conditions (HRT and inflow rate) were 0.016-0.731 of $R^2$ in terms of HRT, and 0.015-0.868 of $R^2$ daily inflow rate. Correlation coefficients of purification amounts per day with hydraulic conditions were 0.173-0.763 of Ra in terms of HRT, and 0.209-0.770 daily inflow rate. Among the correlation coefficients between purification efficiency and hydraulic condition, the percentages of over 0.5 range of $R^2$ were 20% in HRT and in daily inflow rate. However, the percentages of over 0.5 range of correlation coefficients ($R^2$) between purification amount per day and hydraulic conditions were 53% in HRT and 73% in daily inflow rate. The relationships between purificationamount per day and hydraulic condition were more significant than those of purifi-cation efficiency. In this study, high hydraulic conditions (HRT and inflow rate) are not likely to affect significantly the purification efficiency of nutrient. Therefore, the emphasis should be on the purification amounts per day with high hydraulicloadings (HRT and inflow rate) for the improvement of eutrophic reservoir withrelatively low nutrients concentration and large quantity to be treated.

• Journal of Life Science
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• v.18 no.2
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• pp.228-233
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• 2008

Antimicrobial activities of ethanol extracts of about 60 endemic Korean herbs and medicinal plants were investigated. When compared to the group treated with phenoxyethanol as a positive control, the growth of Pseudomonas aeruginosa was inhibited by Styrax japonica, Stevia rebaudiana, Morus Leaf, Coptis chinensis and Slavia miltorrhiza Bunge, the latter showing the strongest inhibition (97%). The growth of Staphylococcus aureus was inhibited by Acorus calamus, Pinus densiflora, Ginkgo biloba, Dryopteris crassirhizoma, Chrysanthemum zawadskii, Slavia miltorrhiza Bunge and Coptis chinensis, the latter showing the strongest inhibition (99%). The growth of yeast type fungi Candida albicans was inhibited to about 35% by Scutellaria baicalensis Geogr and about 33% by Coptis chinensis as determined by paper disk method. The growth of bacterium Malassezia furfur was inhibited by Paeonia lactiflora, Rosa multiflora, Coptis chinensis, Citrus aurantium L. subsp, Paeonia suffruticosa Andrews, and Galla rhois, the latter showing the strongest inhibition (57%). The growth of Propionibacterium acnes was inhibited by Galla rhois, Paeonia lactiflora, Morus bombycis, Cucumis sativus, Stevia rebaudiana, Coptis chinensis and Slavia miltorrhiza Bunge. Interestingly, Coptis chinensis and Slavia miltorrhiza Bunge showed the strongest inhibition (99%) similar to that in postive control. The possible components and their action modes were suggested and discussed.

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.399-402
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• 2002

Angiotensin converting enzyme(ACE) belongs to the class of zinc protease and plays an important role in the regulation of blood pressure. In this experiment, we investigated the inhibitory activities of medicinal plant extracts on ACE. Fifty medicinal plants were selected and the extracts were prepared by refluxing with 70% methanol. Among the extracts, eleven medicinal plant extracts such as Sedum sarmentosum Bunge, Petasites japonicus(s.et z.) Max, Rubus coreanus, Morus bombycis Koidz, Acorus calamus var. angustatus, Glycyrhiza glabra, Equisetum hyemale, Portulaca oleracea L., Prunella vulgaris var. lilacina Nakai, Sorbus commixta Hedl, Allium thunbergii showed more than 50% inhibitory activities, and Paeonia suffruticosa Andr., lnula helenium, Acanthopanax senticosus Harms, Dendrobium moniliforme, Juglans mandshurica, Zizyphus jujuba, Leonurus artemisia, Aster scaber Thunb, Vitex rotundifolia, Platycodon grandiflorum, Prunus persica, Ligularia fischeri showed $40{\sim}49%$ inhibitory activities. Therefore these extracts which contain high ACE inhibitory activities may be useful as antihypertension agents and to the treatment of hypertension.

• Journal of Environmental Impact Assessment
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• v.23 no.6
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• pp.466-476
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• 2014

In this study, 10 species of plants with high adaptability to aquatic environments were compared for selecting plant species suitable for vegetation-based low impact development (LID) facilities. The flooding tolerances of the plants were tested by analyzing their growth status under half-immersion and full-immersion conditions, with varying durations of immersion. In decreasing order of flooding tolerance, the comparative analysis of plant height and leaf width is as follows: Hemerocallis fulva, Juncus effusus var. decipiens, Iris pseudoacorus, Phragmites communis TRIN, Typha orientalis C.Presl, Aster koraiensis Nakai, Iris sanguinea, Equisetum hyemale, Acorus calamus. Specifically, Hemerocallis fulva, Juncus effusus var. decipiens. Iris pseudoacorus showed excellent growth status under both immersion conditions. Iris sanguinea and Equisetum hyemale. withered to death by around day 27 of the experiment, but their flooding tolerance was confirmed to be relatively high. Iris pseudoacoru showed flooding tolerance under the half-immersion condition unlike under the full-immersion condition, when compared on day 21 of the experiment. Aster koraiensis Nakai also thrived better under the half-immersion condition, proving to be a highly immersion-resistant species. On the basis of the results of this experiment, Hemerocallis fulva, Juncus effusus var. decipiens and Iris pseudoacorus, Typha orientalis C.Presl were selected as species suitable for vegetation-based LID facilities.