Browse > Article
http://dx.doi.org/10.7783/KJMCS.2011.19.3.162

Development of SCAR Markers for the Authentication of Acori Rhizoma Based on the Analysis of RAPD and Multiplex-PCR  

Moon, Byeong-Cheol (Center of Herbal Resources Research, Korea Institute of Oriental Medicine)
Ji, Yun-Ui (Center of Herbal Resources Research, Korea Institute of Oriental Medicine)
Lee, Young-Mi (Center of Herbal Resources Research, Korea Institute of Oriental Medicine)
Chun, Jin-Mi (Center of Herbal Resources Research, Korea Institute of Oriental Medicine)
Lee, A-Yeong (Center of Herbal Resources Research, Korea Institute of Oriental Medicine)
Choo, Byung-Kil (College of Agriculture and Life Science, Chonbuk National University)
Kim, Ho-Kyoung (Center of Herbal Resources Research, Korea Institute of Oriental Medicine)
Publication Information
Korean Journal of Medicinal Crop Science / v.19, no.3, 2011 , pp. 162-169 More about this Journal
Abstract
The rhizomes and herbal medicines originating from Acorus gramineus, A. calamus, A. tatarinowii, and A. gramineus var. pusilus, show significant similarity, and the correct identification of species is very difficult. Random Amplified Polymorphic DNA (RAPD) and Sequence Characterized Amplified Region (SCAR) were used to develop a reliable method for identification of these four species. Several distinct SCAR markers were developed from species-specific RAPD amplicons for each species. Furthermore, a useful molecular marker was established for multiplex-PCR, in order to the four species could be distinguished concurrently. These markers allow efficient and rapid identification of closely-related Acorus species and will be useful for standardization of herbal medicines.
Keywords
Acorus Species; Random Amplified Polymorphic DNA (RAPD); Sequence Characterized Amplification Region (SCAR); Molecular Marker; Multiplex-PCR;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Shucher NJ and Carles MC. (2008). Genome-based approaches to the authentication of medicinal plants. Planta Medica. 74:603-623.   DOI   ScienceOn
2 Sugimoto N, Kiuchi F, Mikage M, Mori M, Mizukami H and Tsuda Y. (1999). DNA profiling of Acorus calamus chemotypes differing in essential oil composition. Biological Pharmaceutical Bulletin. 22:481-485.   DOI   ScienceOn
3 Techen N, Crockett SL, Khan IA and Scheffler BE. (2004). Authentication of medicinal plants using molecular biology techniques to compliment conventional methods. Current Medicinal Chemistry. 11:1391-1401.   DOI   ScienceOn
4 Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J and Kuiper M. (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research. 23:4407-4414.   DOI   ScienceOn
5 Wang J, Ha WY, Ngan FN, But PP and Shaw PC. (2001). Application of sequence characterized amplified region (SCAR) analysis to authenticate Panax species and their adulterants. Planta Medica. 67:781-783.   DOI   ScienceOn
6 Weder JK. (2002) Identification of plant food raw material by RAPD-PCR: legumes. Journal of Agricultural Food Chemistry. 50:4456-4463.   DOI   ScienceOn
7 Welsh J and McClelland M. (1991). Genomic fingerprints produced by PCR with consensus tRNA gene primers. Nucleic Acids Research. 19:861-866.   DOI   ScienceOn
8 Williams JG, Kubelik AR, Livak KJ, Rafalski JA and Tingey SV. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research. 18: 6531-6535.   DOI   ScienceOn
9 World Health Organization, (2005). Quality Control Methods for Medicinal Plant Materials. World Health Organization. Geneva, Switzerland. p. 1-115.
10 Yang DY, Fushimi H, Cai SQ and Komatsu K. (2004). Molecular analysis of Rheum species used as Rhei rhizoma based on chloroplast matK gene sequence and its application for identification. Biological Pharmaceutical Bulletin. 27:375- 383.   DOI   ScienceOn
11 Yip PY, Chau CF, Mak CY and Kwan HS. (2007). DNA methods for identification of Chinese medicinal materials. Chinese Medicine. 2:9 (The electronic version of this article is the complete one and can be found online at: http:// www.cmjournal.org/content/2/1/9).   DOI
12 Zhang YB, Shaw PC, Sze CW, Wang ZT and Tong YJ. (2007). Molecular authentication of Chinese herbal materials. Journal of Food Drug Analysis. 15:1-9.
13 Lee MY, Doh EJ, Kim ES, Kim YH, Ko BS and Oh SE. (2008). Application of the multiplex PCR method for discrimination of Artemisia iwayomogi from other Artemisia herbs. Biological Pharmaceutical Bulletin. 29:685-690.
14 Kang CH, Park CB, Choi JS and Choi YG. (2002). Genetic Relationships analysis of Asparagus cochinchinensis Lour collections by random amplified polymorphic DNA. Korean Journal of Medicinal Crop Science. 10:383-391.
15 Kiran U, Khan S, Mirza KJ, Ram M and Abdin MZ. (2010). SCAR markers: A potential tool for authentication of herbal drugs. Fitoterapia. 811:969-976.
16 Korea Food & Drug Administration. (2009). The Korean Herbal Pharmacopoeia. Korea Food & Drug Administration. Seoul, Korea. p. 1005-1006.
17 Lim JH, Jin DC, Sung JS, Bang KH, Kim OT, Cha SW and Park HW. (2007). Discrimination of Astragalus membranaceus (Fisch) Bunge from A. membranaceus (Fisch) Bunge var. mongholicus (Bunge) with SCAR Marker. Korean Journal of Medicinal Crop Science. 15:51-55
18 Ma XQ, Shi Q, Duan JA, Dong TTX and Tsim KWK. (2002). Chemical analysis of Radix astragali (Huangqi) in China: A comparison with its adulterants and seasonal variations. Journal of Agricultural Food Chemistry. 50:4861-4866.   DOI   ScienceOn
19 Ministry of Health, Labour and Welfare. (2011). The Japanese Herbal Pharmacopoeia. Ministry of Health, Labour and Welfare. Tokyo, Japan. p. 1590.
20 Moon BC, Choo BK, Cheon MS, Yoon T, Ji Y, Kim BB, Lee AY and Kim HK. (2010). Rapid molecular authentication of three medicinal plant species Cynanchum wilfordii, Cynanchum auriculatum, and Polygonum multiflorum (Fallopia multiflorum), by the development of RAPD-derived SCAR markers and multiplex-PCR. Plant Biotechnology Reports. 4:1-7.   DOI   ScienceOn
21 Paran I and Michelmore RW. (1993). Development of reliable PCR-based markers linked to downy resistance genes in lettuce. Theoretical and Applied Genetics. 85:985-993.
22 Park CG, Bang KH, Kim OT, Jin DC, Kim DH, Sung JS, Seong NS, Park HW and Lee SC. (2007). Development of SCAR marker for discriminating between violet flowered lines and white flowered lines in Chinese bellflower (Platycodon grandiflorum A.). Korean Journal of Medicinal Crop Science. 15:1-5.
23 Choo BK, Moon BC, Ji Y, Kim BB, Choi G, Yoon T and Kim HK. (2009). Development of SCAR markers for the discrimination of three species of medicinal plants, Angelica decursiva (Peucedanum decursivum), Peucedanum praeruptorum and Anthricus sylvestris, based on the internal transcribed spacer (ITS) sequence and random amplified polymorphic DNA (RAPD). Biological Pharmaceutical Bulletin. 32:24-30.   DOI   ScienceOn
24 Penner GA, Bush A, Wise R, Kim W, Momier L, Kasha K, Laroche A, Scoles G, Molnar SJ and Fedak G. (1993). Reproducibility of random amplified polymorphic DNA (RAPD) analysis among laboratories. PCR Methods Application. 2:341-345.   DOI
25 Chang WT, Thissen U, Ehlert KA, Koek MM, Jellema RH, Hankemeier T, van der Greef J and Wang M. (2006). Effects of growth conditions and processing on Rehmannia glutinosa using fingerprint strategy. Planta Medica. 72:458-467.   DOI   ScienceOn
26 China Pharmacopoeia Committee. (2010). Pharmacopoeia of the People's Republic of China. Part 1. China Chemical Industry Press. Beijing, China. p. 34-59.
27 Dan B, Steven C, Erich S and Andrew G. (2004). Chinese Herbal Medicine Matria Medica. Eastland Press. Washington, USA. p. 283-287.
28 De Feo V, Bruno M, Tahiri B, Napolitano F and Senatore F. (2003). Chemical composition and antibacterial activity of essential oils from Thymus spiulosus Ten. (Lamiaceae). Journal of Agricultural Food Chemistry. 51:3849-3853.   DOI   ScienceOn
29 De Oliveira AC, Richter T and Bennetzen JL. (1996). Regional and racial specifications in Sorghum germplasm assessed with DNA markers. Genome. 39:579-587.   DOI   ScienceOn
30 Dnyaneshwar W, Preeti C, Kalpana J and Bhushan P. (2006). Development and application of RAPD-SCAR marker for identification of Phyllanthus emblica Linn. Biological Pharmaceutical Bulletin. 29:2313-2316.   DOI   ScienceOn
31 Fushimi H, Komatsu K, Isobe M and Namba T. (1997). Application of PCR-RFLP and MASA analyses on 18Sribosomal RNA gene sequence for the identification of three ginseng drugs. Biological Pharmaceutical Bulletin. 20:765-769.   DOI   ScienceOn
32 In DS, Kim YC, Bang KH, Chung JW, Kim OT, Hyun DY, Cha SW, Kim TS and Seong NS. (2005). Genetic relationships of Panax species by RAPD and ISSR analysis. Korean Journal of Medicinal Crop Science. 13:249-253.
33 Bertea CM, Azzolin CM, Bossi S, Doglia G and Maffei ME. (2005). Identification of an EcoRI restriction site for a rapid and precise determination of $\beta$-asarone free Acorus calamus cytotypes. Phytochemistry. 66:507-514   DOI   ScienceOn
34 Kalpana J, Preeti C, Dnyaneshwar W and Bhushan P. (2004). Molecular markers in herbal drug technology. Current Science. 87:159-165.
35 Ahlawat A, Katoch M, Ram G and Ahuja A. (2010). Genetic diversity in Acorus calamus L. as revealed by RAPD markers and its relationship with β-asarone content and ploidy level. Scientia Horticulturae. 124:294-297.   DOI
36 Bang KH, Sung JS, Park CH, Jin DC, Park CG, Yu HS, Park HW and Seong NS. (2004). Discrimination of Atractylodes rhizome white using anatomical characteristics and SCAR markers. Korean Journal of Medicinal Crop Science. 12:53-59.