• 대한한의학회지
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• 제16권2호
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• pp.365-385
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• 1995

In order to prove the antitumor effect of Wekyungtang(WKT) that was originated in Bigeubchunkeumyobang(備急千金要方), Wekyungtang with Houttuyniae Herba(WKT-I) and Wekyungtang with Oldenlandiae diffusae Herba(WKT-II) experimentally, the studies were done, We evaluated the cytotoxic activity against B16- Fo as well as the synergistic effects with anticancer drugs such as cyclophophamide (CPM), cisplatin(CPT) and 5-fluorouracil (5-FU) in vitro and measured body weight, survival time, hematological changes, changes of tissues in G57BL／6 implanted with B16-Fo. The results were obtained as follows: 1. In vitro cytotoxic effect against B16-Fo was shown in all groups as compared with control group, but the concentrations showing inhibitory growth rate below 55% of control was recognized in all concentrations of Wekyungtang(WKT) against B16-Fo and also concentration of $10^4$g／ml above in all group with cyclophophamide (CPM), concentration of $10^3$g／ml in Wekyungtang(WKT-l) with cisplatin(CPT) in synergistic effect, 2. In vivo body and tumor weight were significantly suppressed in all groups as compared with control group 3. The number of platelet, WBC, RBC were significantly increased in all groups, platelet aggregation was significantly increased in WKT-I and WKT-II as compared with control group. 4. In changes of tissues heavy infiltration oh cancer was shown in portal vein, pulmonary tissue, vein, peribronchiole, aveoli, while WKT-I was effective in antihepatic metastasis and WKT-II in pulmonary matastasis. From above results it was concluded that wekyungtang(WKT), wekyungtang with Houttuyniae Herba(WKT-I) and wekyungtang with Oldenlandiae diffusae Herba(WKT -II) had antitumor effect, and also wekyungtang combined with Houttuyniae Herba or Oldenlandiae diffusae Herba were more effective than wekyungtang only and also cyclophophamide (CPM), cisplatin(CPT) showed the more synergistic effect which suggests the necessity of continuous study on the mechanism of antitumor action of Houttuyniae Herba or Oldenlandiae diffusae Herba.

• 한국지반공학회지:지반
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• 제13권1호
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• pp.19-34
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• 1997

과압밀된 점토현장에서 수행한 콘관입시헙 자료를 지구통계학 방법으로 분석하였다. 임의로 위치한 28개의 콘 사운딩 자료(A현장)와 일관되게 위치한 38개치 콘 사운딩 자료(B현장)분석에 이용되었다. 크리깅기법을 이용하여 두 현장의 콘 저항에 대한 베리어그램을 개발했다. 개발된 배리오그램은 현장에서와 수직적 상관거리 및 수평적 상관거리를 규명하는데 이용되었으며 이의 결과는 두 시험결과를 상호비교하려면 샘플링이 수평상관지리 이내에서 수행되어야하는 최적샘 플링설계로 이용된다. 베리오그램의 분석결과는 두 현장의 지질학적 형성이 수직적 및 수평적으로 큰 차이가 없음을 확인할 수 있었다. A현장에서의 크리깅한 콘 저항치를 3차원적으로 나타낸 표면은 콘 저항치의 공간적 변동을 이해하는데 도움을 주며 또한 지구통계학적으로 분식된 3차원적 표면은 분석된 현장의 어느위치에서나 최적의 콘 저항치를 나타낼 수 있다는 것을 의미한다.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제26권1호
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• pp.45-51
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• 2015

Objectives : Children with attention-deficit hyperactivity disorder (ADHD) have been shown to display more inhibitory deficits and executive function deficits. This study investigated the treatment effects of neurofeedback (NF) training on executive function by comparing the results of neuropsychological tests of the trained children at pre- and post-training. Methods : Fifteen children with ADHD, aged 6 to 14 years, participated in the study. The NF treatment consisted of slow cortical potential (SCP) training and these sessions took place once a week. The ADHD children performed 20 sessions of NF training within 6 months. Pre-training and post-training assessments encompassed Continuous Performance Test (CPT), Stroop Test, Children's Color Trails Test I&II (CCTT) and Wisconsin Card Sorting Test (WCST). Results : Patients receiving NF training showed significant improvement in visual commission error and standard deviation of auditory response time on CPT ; and total errors on WCST. But there was no significant improvement in the Stroop test and CCTT. Conclusion : SCP training using NF improves the self-regulatory capacities and impulsivity in ADHD patient, especially impulsivity in visual stimulation tasks. This study showed evidence of clinical efficacy of NF on executive function in ADHD.

• Structural Engineering and Mechanics
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• 제70권1호
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• pp.113-123
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• 2019

In the classical stability investigation of rectangular plates the classical thin plate theory (CPT) is often employed, so omitting the transverse shear deformation effect. It seems quite clear that this procedure is not totally appropriate for the investigation of moderately thick plates, so that in the following the first shear deformation theory proposed by Meksi et al. (2015), that permits to consider the transverse shear deformation influences, is used for the stability investigation of simply supported isotropic rectangular plates subjected to uni-axial and bi-axial compression loading. The obtained results are compared with those of CPT and, for rectangular plates under uniaxial compression, a novel direct formula, similar to the conventional Bryan's expression, is found for the Euler stability stress. The accuracy of the present model is also ascertained by comparing it, with model proposed by Piscopo (2010).

• 한국약용작물학회지
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• 제28권6호
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• pp.395-411
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• 2020

Background: This study investigated the anti-obesity effect of the flavonoid rich fraction (FRF) and its constituent, rutin obtained from the leaf of Morus alba L., on the lipid accumulation mechanism in 3T3-L1 adipocyte and C57BL/6 mouse models. Methods and Results: In Oil Red O staining, FRF (1,000 ㎍/㎖) treatments showed inhibition rate of 35.39% in lipid accumulation compared to that in the control. AdipoRedTM assay indicated that the triglyceride content in 3T3-L1 adipocytes treated with FRF (1,000 ㎍/㎖) was reduced to 23.22%, and free glycerol content was increased to 106.04% that of the control. FRF and its major constituent, rutin affected mRNA gene expression. Rutin contributed to the inhibition of Sterol regulatory element binding protein-1c (SREBP-1c) gene expression, and inhibited the transcription factors SREBP-1c, peroxisome proliferator-activated receptor gamma (PPAR-γ), CCAAT/enhancer binding protein α (C/EBPα), fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC). In addition, the effect of FRF administration on obesity development in C57BL/6 mice fed high-fat diet (HFD) was investigated. FRF suppressed weight gain, and reduced liver triglyceride and leptin secretion. FRF exerted potential anti-inflammatory effects by improving insulin resistance and adiponectin levels, and could thus be used to help counteract obesity. The mRNA expressions of PPAR-γ, FAS, ACC, and CPT-1 were determined in liver tissue. Quantitative real-time PCR analysis was also performed to evaluate the expression of IL-1β, IL-6, and TNF-α in epididymal adipose tissue. Compared to the control group, mice fed the HFD showed the up-regulation in PPAR-γ, FAS, IL-6, and TNF-α genes, and down-regulation in CPT1 gene expression. FRF treatement markedly reduced the expression of PPAR-γ, FAS, IL-6, and TNF-α compared to those in HFD control, whereas increased the expression level of CPT1. Conclusions: These results suggest that the FRF and its major active constituent, rutin, can be used as effective anti-obesity agents.

• 한국지반공학회논문집
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• 제26권11호
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• pp.47-61
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• 2010

연직배수공이 매설되는 점토지반에서 압밀속도를 예측하기 위해서는 다양한 종류의 고급 실내 및 현장토질실험을 수행하여 합리적으로 수리학적 특성치를 결정하는 것이 바람직하다. 그러나 실무에서와 같이 보편적인 수행할 경우에 수리학적 특성을 합리적으로 규명하는 것이 우선 필요하다. 이를 위하여 낙동강 허구의 범람원 지역에 속하는 5개 지점을 선정하여 수많은 CPT 소산시험, 보편적인 실내 투수 및 압밀실험을 실시하였으며, 그들을 이용하여 종합적으로 분석하였다. 그 결과, 이 점토의 수평방향 압밀계수($C_{h,NC}$) 및 투수계수($k_{h,OC}$)는 Balight & Levadoux(1986)의 제안법을 적용한 CPT소산시험결과가 가장 적합한 것으로 나타났다. 여기서 $C_{h,NC}$와 $k_{h,OC}$는 각각 $0.4{\sim}3.0\;cm^2/sec$ 및 $(1{\sim}3){\times}10^{-9}m/sec$ 범위에 있으며, 두 값은 깊이에 따라 약간씩 증가 및 감소하는 경향을 보였다. 이러한 퇴적환경을 잘 반영하는 것으로 해석되었다.

• 대한본초학회지
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• 제33권3호
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• pp.63-70
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• 2018

Objective : Previous studies showed that water extract of Plantago asiatica (Plantaginis asiaticae Folium, PAF) significantly controlled in body weights, adipose tissue weights and blood lipid profiles in obese C57BL/6 mice. To investigate the mechanism of anti-obesity action of PAF, expressions of obesity-related proteins were identified such as p-AMPK and p-ACC in hypothalamus, UCP-1 in brown adipose tissue, p-AMPK, p-ACC, SREBP-1c, $PPAR{\gamma}$, HMGCR and CPT-1 in liver. Method : Five-weeks old male C57BL/6 mice were divided into 5 groups; ND (normal diet + 0.9% saline), HFD (high-fat diet + 0.9% saline), PC (high-fat diet+Garcinia cambogia 500 mg/kg), PAF 100 and 300 (high-fat diet + PAF 100 or 300 mg/kg). PAF was treated orally for 6 weeks. The protein expression of AMPK, p-AMPK, ACC, p-ACC, $PPAR{\gamma}$, SREBP-1c, HMGCR, CPT-1 and UCP-1 were identified by expression levels of proteins through western blot analysis. Result : The results showed that protein expressions on hypothalamic p-AMPK and p-ACC did not differ between the HFD and PAF groups. In addition, PAF did not affect the increase of UCP-1 in brown adipose tissue. The protein expression levels of hepatic p-AMPK, p-ACC and CPT-1 increased in PAF groups compared to HFD group. And those of $PPAR{\gamma}$, SREBP-1c and HMG-CoA decreased in PAF groups compared to HFD group. Conclusion : These results suggest that the PAF administration induce weight loss via inhibition of lipid metabolism-related protein expressions in hepatic tissues. Therefore, PAF could be used as a potent material of anti-obesity products for prevention and treatment of obesity.

• Asian-Australasian Journal of Animal Sciences
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• 제33권3호
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• pp.390-397
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• 2020

Objective: The objective of this study was to measure the special expression pattern of lipid metabolism genes and investigate the molecular mechanisms underlying intramuscular fat (IMF) deposition in Longissimus dorsi muscle of Laiwu pigs. Methods: Thirty-six pigs (Laiwu n = 18; Duroc×Landrace×Yorkshire n = 18) were used for the measurement of the backfat thickness, marbling score, IMF content, and expression of lipid metabolism genes. Results: Significant correlations were found between IMF content and the mRNA expression of lipid metabolism genes. Of the 14 fat deposition genes measured, fatty acid synthase (FASN) showed the strongest correlation (r = 0.75, p = 0.001) with IMF content, and of the 6 fat removal genes, carnitine palmitoyl transferase 1B (CPT1B) exhibited the greatest negative correlation (r = -0.66, p = 0.003) with IMF content in Laiwu pig. Multiple regression analysis showed that CPT1B, FASN, solute carrier family 27 member 1 (SLC27A1), and fatty acid binding protein 3 (FABP3) contributed 38% of the prediction value for IMF content in Laiwu pigs. Of these four variables, CPT1B had the greatest contribution to IMF content (14%) followed by FASN (11%), SLC27A1 (9%), and FABP3 (4%). Conclusion: Our results indicate that the combined effects of an upregulation in fat deposition genes and downregulation in fat removal genes promotes IMF deposition in Laiwu pigs.

• Journal of Veterinary Science
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• 제22권4호
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• pp.55.1-55.17
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• 2021

Background: Naringenin and its glycoside naringin are well known citrus flavonoids with several therapeutic benefits. Although the anti-adipogenic effects of naringenin and naringin have been reported previously, the detailed mechanism underlying their anti-adipogenesis effects is poorly understood. Objectives: This study examined the anti-adipogenic effects of naringenin and naringin by determining differential gene expression patterns in these flavonoids-treated 3T3-L1 adipocytes. Methods: Lipid accumulation and triglyceride (TG) content were determined by Oil red O staining and TG assay. Glucose uptake was measured using a 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose fluorescent d-glucose analog. The phosphorylation levels of AMP-activated protein kinase (AMPK) and acetyl Co-A carboxylase (ACC) were observed via Western blot analysis. Differential gene expressions in 3T3-L1 adipocytes were evaluated via RNA sequencing analysis. Results: Naringenin and naringin inhibited both lipid accumulation and TG content, increased phosphorylation levels of both AMPK and ACC and decreased the expression level of 3-hydroxy-3-methylglutaryl CoA reductase (HMGCR) in 3T3-L1 adipocytes. RNA sequencing analysis revealed that 32 up-regulated (> 2-fold) and 17 down-regulated (< 0.6-fold) genes related to lipid metabolism, including Acaca, Fasn, Scd1, Mogat1, Dgat, Lipin1, Cpt1a, and Lepr, were normalized to the control level in naringenin-treated adipocytes. In addition, 25 up-regulated (> 2-fold) and 25 down-regulated (< 0.6-fold) genes related to lipid metabolism, including Acaca, Fasn, Fabp5, Scd1, Srebf1, Hmgcs1, Cpt1c, Lepr, and Lrp1, were normalized to the control level by naringin. Conclusions: The results indicate that naringenin and naringin have anti-adipogenic potentials that are achieved by normalizing the expression levels of lipid metabolism-related genes that were perturbed in differentiated 3T3-L1 cells.

• 사상체질의학회지
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• 제17권3호
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• pp.76-81
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• 2005

1. Objectives The purpose of this study is to find the difference of response to visual or auditory stimulus in Sasang Constitutional groups. 2. Methods Continuous performance test (CPT)-ADS program- was measured in 44 females in 20's. The mean and standard deviation of response time were measured and analysed statically in each group of Sasang Constitution. 3. Results There were significant results in the average of response time. The average of the response time to the auditory stimulus in Soyangin was shorter then that of in Soeumin 4. Conclusions The results suggest that there could be differences in the pattern of response to circumstantial stimulus in Sasang Constitution.