• Korean journal of applied entomology
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• v.55 no.3
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• pp.245-256
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• 2016

Elytra of Harmonia axyridis exhibit varied color patterns. In the present study, we deciphered the genetic basis for intraspecific diversity of elytra color patterns in H. axyridis, using amplified fragment length polymorphism (AFLP). Twenty-eight AFLP reactions were performed to generate a total of 2,741 bands. Of these, 20 bands were polymorphic for each color pattern. The polymorphic bands showed differences of genetic character among different color patterns of H. axyridis. Among them, ten candidate AFLP markers were color-linked. S1, S2, and S20 markers were detected in Succinea 1 and 2 variants of H. axyridis, whereas S3 and S5 were specifically detected in the Conspicua variant. S15, S18, and S19 were specific to the Succinea 2 variant. Polymerase chain reaction (PCR) products of these ten AFLP markers were sequenced. BLAST analysis of these sequences against the GenBank database revealed their homology to DNA fragments of unknown function. Based on the color-linked AFLP markers, sequence characterized amplified region (SCAR) markers were designed for PCR amplification of genomic DNA. Of the ten AFLP markers, five were successfully converted into SCAR markers, which could discriminate elytra color polymorphism in H. axyridis.

• Korean journal of applied entomology
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• v.48 no.2
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• pp.189-195
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• 2009

This study was performed to investigate the emergence timing and daily emergence from the different tree species logs (Korean white pine, Pine, and Japanese larch) at Cheongwon, Chungcheongbuk-do from 2006 to 2008. Pine sawyer adults began to emerge on early May (2${\sim}$10th day) and finished on mid-June (4${\sim}$20th day) in the Korean white pine and pine logs. However, pine sawyer adults began to emerge on mid-May (13th day) and finished late May (27th day) in Japanese larch logs, Japanese larch logs shows shorter emergence timing of pine sawyer adult compare to those of other two tree species. Emergence timing shows no difference between males and females. Sex ratios of emerged adult in 2007 were 0.55 from Korean white pine, 0.46 from Pine, and 0.59 from Japanese larch; it shows no difference among tree species. Female and male adults emerged throughout 24 hrs; 22.8% of the total adults emerged from 12${\sim}$14:00 (22.8%) and adults emerged lowest from 06${\sim}$08:00. Of the adults emerged from the logs, 98.9% was univoltine and the rest was biennial which emerged next year.

• Korean journal of applied entomology
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• v.56 no.1
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• pp.29-39
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• 2017

To compare the features of ground-dwelling invertebrates according agroecosystems, we selected paddy fields, dry fields, orchards in the Eastern region of Korea. The surveys were performed by using pit-fall traps twice per year from 2013 to 2015. Total 6,420 individuals of 172 species belonging to 13 orders, 58 families were investigated in the Eastern region, the species of Hymenoptera (38.26%), Orthoptera (16.28%) accounted large portion of the communities. In the geographical observation, invertebrates were caught was 2,983 individuals in Gyeongsangnam-do, the diversity index of Gyeongsangbuk-do community was higher than of the others and abundance and species richness of paddy field were higher than from dry field or orchard. To understand the relation between taxonomic groups and environmental factors, we carried out the canonical correspondence analysis and hierarchical clustering. As a result, Homoptera, Blattaria, Isoptera, and Coleoptera were positively related to soil pH, soil temperature, and moisture contents, and negatively related to the others. Invertebrate community also were patterned dependently by type of ecosystems. This results were shown that distribution of invertebrates is a few influenced the relationship of the space habituated invertebrates and environmental factors.

• Korean journal of applied entomology
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• v.41 no.2
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• pp.123-128
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• 2002

This study was carried out to test the effects of temperatures between 2$0^{\circ}C$ and 3$0^{\circ}C$ on the reproduction and development of the rice water weevil, Lissorhoptrus oryzophilus. Preoviposition periods were much longer (17.2 to 51.0 days) in the overwintering adult females collected in March than those collected in May, regardless of temperature. Oviposition periods, however, were longer (16.9 to 22.0 days) in the adult females collected in May than those collected in March at the same temperatures. The longer oviposition period observed in the females collected in May were directly associated with higher fecundity. Egg periods were shortened from as temperature increased, but the hatching rate was highest (100%) at 27$^{\circ}C$. The developmental periods from egg to adult were shortened as temperature increased : from 77.9 days at 2$0^{\circ}C$ to 38.3 days at 3$0^{\circ}C$. The developmental zero point temperature (T) and the total effect temperature (K) for egg were 16.3$^{\circ}C$ and 62.1 dgree days, respectively. The T and K from egg to adult emergence were 13.9$^{\circ}C$ and 577.6 dgree days, respectively. The adult females of the first generation did not oviposit at 2$0^{\circ}C$, but did at $25^{\circ}C$ and 3$0^{\circ}C$. The intrinsic rate of natural increase (r$_{m}$) increased as temperature augmented. Net reproductive rate (Ro) per generation was highest (75.3) at $25^{\circ}C$.>.

• Korean journal of applied entomology
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• v.42 no.4
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• pp.307-313
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• 2003

Japanese pine sawyer, Monochamus alternatus, is a primary vector of pinewood nematode, Bursaphelenchus xylophilus in Korea. Emergence characteristics of the adults were studied in Jinju, Korea using dead pine logs in which the larvae of the sawyer overwintered from 1999 to 2002. Emergence data showed that the adults began to emerge out of the logs in sunny place from May the 15th in average of the four years. Cumulative emergence ratio (CER) reached at 50% at mid-June, and the emergence terminated by early or late July depending on years. In shady place, however,50% CER was reached 17 days later than in sunny place. The 50％ CER of males reached 1 to 4 days earlier than that of females depending on years. Of the adults emerged from the logs collected from February to April in 2001, 97.6％ was univoltine, and the rest was biennial which emerged from May to July of the next year. Female and male adults emerged throughout 24 hours; 32.3% of the total adults emerged from 8 to 12 a.m. It took 68.0 seconds for the adults to escape from pine logs.

• Korean journal of applied entomology
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• v.42 no.4
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• pp.315-321
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• 2003

Larval entrance and adult emergence holes of Japanese pine sawyer (Monochamus alternatus), primary vector of pinewood nematode (Bursaphelenchus xylophilus), were measured in dead pine logs from 1999 to 2002. Their distributions within pine log were also analyzed. More numbers of entrance and emergence holes were distributed on crown than trunk part as 56.2 and 27.7 holes/m$^2$, respectively Higher proportions of entrance (27.5％) and emergence holes (22.4%) were distributed on the log with 8 to 10 cm diameter; the larger or the smaller logs had fewer holes. Surface area of entrance hole was 65.8 $\textrm{mm}^2$ and diameter of emergence holes was 7.0 mm in average. Average depth of pupal chamber was 24.8 mm from surface to the bottom of the chamber, and its volume ranged from 200 to 2.000 ㎣ Average distance between entrance and emergence holes on bark surface was 3.3 cm. Gallery length from the beginning of entrance hole to the end of emergence hole was 46.2 mm.

• Korean journal of applied entomology
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• v.47 no.1
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• pp.9-15
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• 2008

This study was conducted to investigate the seasonal occurrence of chestnut weevil, Curculio sikkimensis. The chestnuts infected by the weevils were collected from Gongju, Buyeo, and Cheongyang in Chungnam Province, Republic of Korea. Investigation was focus on the escape period of chestnut weevil larvae from the fruits, invading time and vertical distribution in soil, survival rates of larvae during overwintering, emergence period, emergence rate, and sex ratio and longevity of adults. For precocious species, larvae escaped the chestnut from the mid-September to the early-October, middle species and slow species, were escaped from the late-September to the mid-October and from the early-October to the mid-November. After escaping from the chestnut fruits all of the larvae burrowed into the soil within 35 minutes. Overwintering larvae inhabited in the range of $0{\sim}48cm$ from the soil surface and highly distributed in the range of $18{\sim}36cm$. The 74.1 % of wintering chamber were distributed within $18{\sim}36cm$ from the surface. Survival rates observed were 38.0% in 1st year, 16.0% in 2nd years, and 2% in 3rd years, respectively. Seasonal occurrence period of C. sikkimensis was from the early-Aug. to early-Oct. and the optimal occurrence period was the early and mid-Sept. Emergence rate decreased to 8.4% in 1st year, 3.6% in 2nd years, and 0.8% in 3rd years, respectively. Sex ratio was showed in the range of 0.51-0.55. It is female biased ratio. Longevity of adults was 9.9 days for female and 8.9 days for male.

• Korean journal of applied entomology
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• v.47 no.1
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• pp.23-29
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• 2008

The biology of indian meal moth and maize weevil on the trehalose gene introduced rice (var 'Nakdong') (T-Nakdong) was compared to that on the rice without gene introduction (Nakdong) at $28{\pm}1.0^{\circ}C$. Development of the both two insects was significantly delayed; on T-Nakdong, the developmental periods of female moth $({\pm}SE)$ and weevil were $38.46{\pm}0.42\;and\;36.38{\pm}0.28days$, respectively. Whereas those on Nakdong were $36.38{\pm}0.28\;and\;34.33{\pm}0.18$, respectively. Net reproduction rate of Indian meal moth on T-Nakdong $(3.0{\pm}0.14)$ was significantly lower than that on Nakdong $(9.25{\pm}0.13)$, due to the lower emergence rate $(21.08{\pm}0.04%\;vs\;48.30{\pm}0.06%)$. The life table statistics of maize weevil on T-Nakdong was estimated to be similar to those on Nakdong. The delayed development of T-Nakdong suggested that the factor for development of the insects could be changed by the introduction of trehalose gene into the rice variety.

• Asian Journal of Turfgrass Science
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• v.19 no.1
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• pp.39-46
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• 2005

The 21 strains of Korean entomopathogenic nematodes, {Heterorhabditis bacteriophora Hamyang strain(HbH), Heterorhabditis sp. 202, 205, 217, Heterorhabdiris sp. KCTC 0991BP strain, Steinernema carpocapsae Pocheon(ScP), S. longicaudum Gonaju, S. longicaudum Nonsan, Steinernema sp. 7,24, 52, 55, 60, 64, 206, 207, 209, 210, 219, and 227 strain} were evaluated for the control of a turfgrass insect pest, Exomala orientalis. Heterorhabditis spp. showed higher pathogenicity than Steinernema spp. against 3rd instar larvae of E. orientalis with $55\%$ mortality by Heterorhabditis sp. 202 strain and $50\%$ by HbH and Heterorhabditis sp.205 strain at the rate of 200 infective juveniles per larva 14 days later after treatment. The number of infective juveniles of Korean entomopathogenic nematodes in 3rd instar larvae of E. orientalis was higher in Heterorhabditis spp. than in Steinernema spp.. In general, numbers of produced infective juveniles of three species were much higher, i.e., Heterorhabditis sp.202 strain produced 273,064 infective juveniles, S. carpocapsae Pocheon strain 273,043, and Heterorhabditis sp. 217 strain 248,887, respectively.

• Asian Journal of Turfgrass Science
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• v.23 no.1
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• pp.45-60
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• 2009

Occurrence ecology and possibility of mass trapping of Popillia quadriguttata, one of the most serious turfgrass insect pest in domestic golf courses was investigated using pheromone traps in Gimpo and Jinhae. Peak period of adult occurrence of P. quadriguttata was late June and early July in Gimpo Seaside Country Club from 2006 to 2008. It was early and middle July in Youngwon Country Club in 2006. The first catched day of P. quadriguttata adult was 20 June, while the last day was 10 September in Gimpo Seaside Country Club, 2006. Mean catched number of P. quadriguttata adult in Japanese beetle pheromone trap per day in peak period of occurrence were 18.2, 25.7, 29.0, and 15.7 at 2005, 2006, 2007 and 2008 respectively. Mean catched number of P. quadriguttata adult in Japanese beetle pheromone trap were more in fairways or roughs than tees or greens. Possibility of control of scarab beetle using pheromone trap with pesticide treatment was investigated against oriental beetle, Blitopertha orientalis in Dongrae Benest Golf Club. Mean number of dead oriental beetle adult were more higher(2.9 folds) in fenitrothion EC treatment than fenitrothion EC treatment after set oriental beetle pheromone trap.