• Title/Summary/Keyword: CLP

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Effect of Medicinal Herbs Extracts on Physicochemical Characteristics of Cooked Rice (복합 약용식물 추출물이 쌀밥의 이화학적 특성에 미치는 영향)

  • Cho, Yong-Sik;Lee, Kyung-Ha;Ha, Hyun-Jee;Park, Shin-Young;Choi, Yoon-Hee;Kim, Eun-Mi
    • Journal of Applied Biological Chemistry
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    • v.53 no.4
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    • pp.219-224
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    • 2010
  • This study was conducted to investigate the effect of medical herbs extracts on physicochemical characteristics of cooked rice. Three types (CLP 1, CLP 2, CLP 3) of extracts were prepared using 11 medicinal herbs, respectively. Polished rice was cooked with different concentration of medicinal herbs extracts by electric cooker. The moisture contents in cooked rice were decreased by increased concentration of herbs extracts, while total polyphenol contents were significantly increased. DPPH radical scavenging activities of cooked rice obviously were increased as herb extracts level increased. The consistency and setback value of the rice flour was reduced at all of the extracts, while breakdown and initial pasting temperature was increased. Color of cooked rice was shown different comparing to the control. However, the hardness, adhesiveness, springiness, cohesiveness and gumminess of cooked rice was increased depending on the concentration of extracts. These results showed that medicinal herbs extracts, which can benefit human health, is inducing the changes of physicochemical characteristics of cooked rice.

Freeze Tolerance Enhanced by Antifreeze Protein in Plant

  • Hwang, Cheol-Ho;Park, Hyun-Woo;Min, Sung-Ran;Liu, Jang-Ryol
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.4
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    • pp.339-343
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    • 2000
  • When plants are exposed to subfreezing temperatures ice crystals are forming within extracelluar space in leaves. The growth of ice crystal is closely related to the degree of freezing injury. It was shown that an antifreeze protein binds to an ice nucleator through hydrogen bonds to prevent growth of ice crystal and also reduces freezing damage. The antifreeze proteins in plants are similar to PR proteins but only the PR proteins induced upon cold acclimation were shown to have dual functions in antifreezing as well as antifungal activities. Three of the genes encoded for CLP, GLP, and TLP were isolated from barley and Kentucky bluegrass based on amino acid sequence revealed after purification and low temperature-inducibility as shown in analysis of the protein. The deduced amino acid of the genes cloned showed a signal for secretion into extracellular space where the antifreezing activity sup-posed to work. The western analysis using the antisera raised against the antifreeze proteins showed a positive correlation between the amount of the protein and the level of freeze tolerance among different cultivars of barely. Besides it was revealed that TLP is responsible for a freeze tolerance induced by a treatment of trinexapac ethyl in Kentucky bluegrass. Analysis of an overwintering wild rice, Oryza rufipogon also showed that an acquisition of freeze tolerance relied on accumulation of the protein similar to CLP. The more direct evidence for the role of CLP in freeze tolerance was made with the analysis of the transgenic tobacco showing extracellular accumulation of CLP and enhanced freeze tolerance measured by amount of ion leakage and rate of photosynthetic electron transport upon freezing. These antifreeze proteins genes will be good candidates for transformation into crops such as lettuce and strawberry to develop into the new crops capable of freeze-storage and such as rose and grape to enhance a freeze tolerance for a safe survival during winter.

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Regulation of Activity of the Response Regulator RssB (Response Regulator RssB의 활성 조절)

  • Park, Hee Jeong;Bang, Iel Soo
    • Korean Journal of Microbiology
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    • v.49 no.3
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    • pp.215-220
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    • 2013
  • Against environmental stresses, many bacteria utilize the alternate sigma factor RpoS that induces transcription of the specific set of genes helpful in promoting bacterial survival. Intracellular levels of RpoS are determined mainly by its turnover through proteolysis of ClpXP protease. Delivery of RpoS to ClpXP strictly requires the adaptor protein RssB. The two-component-type response regulator RssB constantly interacts with RpoS, but diverse environmental changes inhibit this interaction through modification of RssB activity, which increases RpoS levels in bacteria. This review discusses and summarizes recent findings on regulatory factors in RssB-RpoS interactions, including IraD, IraM, IraP anti-adaptor proteins of RssB and phosphorylation of N-terminal receiver domain of RssB. New information shows that the coordinated regulation of RssB activity in controlling RpoS turnover confers efficient bacterial defense against stresses.

Performance Analysis of WATM-OFDM/l6QAM System in Frequency Selective Rayleigh Fading Channel (주파수 선택성 레일리 페이텅 통신로에서 WATM-OFDM/16QAM 시스템의 성능 분석)

  • 박기식;이영춘;강영흥;김언곤;조성언
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.4 no.3
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    • pp.635-642
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    • 2000
  • We have been derived theoretically the SER's and CLP's of Wireless ATM (WATM) cells employing an OFDM/16QAM modulation scheme in wireless channel modeled as a frequency selective Rayleigh fading channel. The amount of the performance improvement of WATM- OFDM/16QAM systems adopting various coding techniques has been evaluated. In frequency selective Rayleigh fading channel, considering CLP : $10^{-3}$ as a criterion, it is observed that the performance improvement of about 14 dB is obtained in terms of $E_b/N_o$ by employing an OFDM scheme. It is also confirmed that convolutional coding technique gives better performance than the other coding techniques. Especially, when the convolutional codes are adopted to WATM-OFDM/16QAM systems, voice transmission services are sufficiently available with 5 dB of $E_b/N_o$.

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Effects of frequency ratio on bridge aerodynamics determined by free-decay sectional model tests

  • Qin, X.R.;Kwok, K.C.S.;Fok, C.H.;Hitchcock, P.A.
    • Wind and Structures
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    • v.12 no.5
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    • pp.413-424
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    • 2009
  • A series of wind tunnel free-decay sectional model dynamic tests were conducted to examine the effects of torsional-to-vertical natural frequency ratio of 2DOF bridge dynamic systems on the aerodynamic and dynamic properties of bridge decks. The natural frequency ratios tested were around 2.2:1 and 1.2:1 respectively, with the fundamental vertical natural frequency of the system held constant for all the tests. Three 2.9 m long twin-deck bridge sectional models, with a zero, 16% (intermediate gap) and 35% (large gap) gap-to-width ratio, respectively, were tested to determine whether the effects of frequency ratio are dependent on bridge deck cross-section shapes. The results of wind tunnel tests suggest that for the model with a zero gap-width, a model to approximate a thin flat plate, the flutter derivatives, and consequently the aerodynamic forces, are relatively independent of the torsional-to-vertical frequency ratio for a relatively large range of reduced wind velocities, while for the models with an intermediate gap-width (around 16%) and a large gap-width (around 35%), some of the flutter derivatives, and therefore the aerodynamic forces, are evidently dependent on the frequency ratio for most of the tested reduced velocities. A comparison of the modal damping ratios also suggests that the torsional damping ratio is much more sensitive to the frequency ratio, especially for the two models with nonzero gap (16% and 35% gap-width). The test results clearly show that the effects of the frequency ratio on the flutter derivatives and the aerodynamic forces were dependent on the aerodynamic cross-section shape of the bridge deck.

An Integrated GFR Buffer Management Algorithm or improving Internet Traffic Performance over ATM Networks (ATM 망에서 인터넷 트래픽 성능 향상을 위한 GFR 통합 버퍼 관리 기법)

  • Jeong Kwang-Il;Kim Kwan-Woong;kwak Hyun-min;Kim Nam-Hee;Chung Hyung-Taek;Chae Kyun-Shik;Chon Byoung-Sil
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.41 no.1
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    • pp.73-82
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    • 2004
  • As a new service category to better support TCP traffic in ATM networks, the Guaranteed Frame Rate (GFR) service category aims to support minimum cell rate guarantee, fairly distribute available bandwidth while keeping the simplicity of Unspecified Bit Rate (UBR). In this paper, we proposed a buffer management scheme which uses the per-VC accounting of single FWO queue and capable of supporting both GFR.1 and GFR.2 conformance definition. The proposed buffer management deal with GFR.1 and GFR.2 conformance definitions differentially by controlling the number of CLP=0 cell and CLP=1 cell which are occupying buffer space. The simulation results show that our proposed scheme satisfies the requirements of GFR services as well as improves total fairness index and each conformance definition fairness index.

Effect of Nicardipine on the Pharmacokinetic Parameters of Cyclosporine in Rat (흰쥐에서 Cyclosporine의 약동학적 지표에 대한 Nicardipine의 영향)

  • 김희규;강주섭;이창호;신인철
    • Biomolecules & Therapeutics
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    • v.6 no.4
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    • pp.389-394
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    • 1998
  • Cyclosporine (CsA) is a major immunosuppressive drug used widely to prevent organ allograft rejection. fits potential organotoxicity by prolonged use is known to cause both direct tissue damage and indirect pharmacokinetic interactions with other drugs. This study was performed to determine the effect of nicardipine (NCP) on the pharmacokinetic parameters of CsA in Sprague-Dawley rats. Each rat was administered with CsA in saline-treated group or in NCP-treated group which was pretreated with NCP (5 mg/kg/12 hours, i.p.) for 6 days. The plasma CsA concentration were analyzed by reversed HPLC: UV system at 0.5, 1, 2, 4, 6, and 8 hours after bolus injection of CsA (10 mg/kg). Pharmacokinetic parameters (mean$\pm$ SD, n=7) such as initial plasma concentration (C(0)), mean residence time (MRT), steady-state volume of distribution (Vdss), terminal half-life (t$\frac{1}{2}$($\beta$)) and plasma clearance (CLp) of CsA in each groups (saline-group vs NCP-group) were determined as follows: C(0) (5.66$\pm$ 1.98 vs 17.98$\pm$2.36, p<0.01); Vdss (2.68$\pm$ 1.6 vs 0.94 $\pm$ 0.25, p<0.01); CLp (0.53 $\pm$0.18 vs 0.21 $\pm$0.06, p<0.01). Therefore, Our results indicate that nicardipine significantly affects the pharmacokinetic parameters of cyclosporme, especially C(0), Vdss, and CLp in NCP-treated group. We suggest that the significant pharmacokinetic interaction between cyclosporine and nicardipine should be considered and cyclosporine level should be closely monitored and dosage reduction made as necessary in clinical situation that was coadministered with CsA and NCP.

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Insight Into Genes Involved in the Production of Extracellular Chitinase in a Biocontrol Bacterium Lysobacter enzymogenes C-3

  • Choi, Hoseong;Kim, Hyun Jung;Lee, Jin Hee;Kim, Ji Soo;Park, Seur Kee;Kim, In Seon;Kim, Young Cheol
    • The Plant Pathology Journal
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    • v.28 no.4
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    • pp.439-445
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    • 2012
  • The chitinase producing Lysobacter enzymogenes C-3 has previously been shown to suppress plant pathogens in vitro and in the field, but little is known of the regulation of chitinase production, or its role in antimicrobial activity and biocontrol. In this study, we isolated and characterized chitinase-defective mutants by screening the transposon mutants of L. enzymogenes C-3. These mutations disrupted genes involved in diverse functions: glucose-galactose transpoter (gluP), disulfide bond formation protein B (dsbB), Clp protease (clp), and polyamine synthase (speD). The chitinase production of the SpeD mutant was restored by the addition of exogenous spermidine or spermine to the bacterial cultures. The speD and clp mutants lost in vitro antifungal activities against plant fungal pathogens. However, the gluP and dsbB mutants showed similar antifungal activities to that of the wild-type. The growth of the mutants in nutrient rich conditions containing chitin was similar with that of the wild-type. However, growth of the speD and gluP mutants was defective in chitin minimal medium, but was observed no growth retardation in the clp and dsbB mutant on chitin minimal medium. In this study, we identified the four genes might be involved and play different role in the production of extracellular chitinase and antifungal activity in L. enzymogenes C-3.

Le Fort I maxillary osteotomy for cleft lip and palate patients (구순구개열 환자를 위한 상악 악교정 수술)

  • Shin, Young-Min;KWON, Tae-Geon
    • The Journal of the Korean dental association
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    • v.53 no.7
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    • pp.468-475
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    • 2015
  • In cleft lip and palate (CLP) patients, there are various degree of residual maxillofacial deformities in adolescent period. Usually, orthoganthic surgery for the cleft patients needs Le Fort I osteotomy and/or mandibular set-back surgery. Previous report from other institute had been shown that there is significant relapse after maxillary movement after Le Fort I osteotomy when the surgical advancement of the maxilla was over than 5 or 7mm in average. Recent comphrehensive report showed that most of the relapse was happened within 1 year and the total horizontal relapse of the maxilla was as high as 30% in average. Therefore, overcorrection is needed in maxillary surgery for cleft patients. Another concerns for cleft orthognathic surgery is the anatomical variation in pterygomaxillary region in cleft patients compared to control patients. Patients with CLP had larger and thicker pterygomaxillary dimensions, and the results imply that careful attention to pterygomaxillary anatomy is needed in patients with CLP undergoing Le Fort I surgery. This article reviews the pre and postoperative considering factors for orthognathic surgery for CLP patients.