• 한국작물학회지
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• 제45권3호
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• pp.151-157
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• 2000

옥수수수염에 함유되어 있는 maysin 및 유사 flavonoid 물질의 분리 및 정제법을 확립하여 신품종 육성의 기초적인 자료를 제공하고자 본 시험을 수행하였으며 얻어진 결과를 요약하면 다음과 같다. 1. Preparative $C_{18}$ 컬럼에서 10% MeOH에 용출된 물질은 neochlorogenic acid, chlorogenic acid 및 4-caffeoylquinic acid였으며 30% MeOH로 용출된 물질은 rhamnosyl isoorientin이었고 maysin은 50%의 MeOH에서 용출되었다. 2. Silicic acid 컬럼으로 maysin 조추출물의 1차 정제시 100% ethyl acetate 500$m\ell$로 컬럼에 흡착되어 있는 maysin을 용출시켰으며, 이때 수거된 maysin의 순도는 75% 이상에 해당하였고, $C_{18}$ 컬럼($\frac{1}{2}$$\times$43")으로 maysin의 2차 정제시 maysin의 순도는 95% 이상에 달하였다. 3. FAB-MS에 의한 maysin의 분자량은 577M＋H m/z이고, fragmentation으로 보아 431M＋H m/z은 rhamnose에 해당하였고, $^1$H 및 $^{13}$C NMR에 의한 spectrum을 확인한 결과 분리한 물질이 maysin임을 확인할 수 있었다. 있었다.

• 상하수도학회지
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• 제23권4호
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• pp.431-438
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• 2009

The purpose of this study was to evaluate the performances of zero-valent iron (ZVI) combined SBR (Z-SBR) process in decolorization and organic removal of synthetic dye wastewater. The batch test for optimizing the operation parameters of ZVI column showed that the appropriate EBCT was around 11 min and the pH of the dye wastewater was below 7.0. During the step increase of influent color unit from 300 to 1,000cu, about 53 to 79% decolorization efficiency could be achieved in control SBR (C-SBR, without ZVI column), which resulted from destroying azo bond of synthetic dye in anaerobic condition. For the same influent color loading, Z-SBR showed always higher decolorization efficiency than C-SBR with an aid of ZVI reducing power. The TCOD concentration in Z-SBR effluent was 20-30mg/L lower than C-SBR effluent although the TCOD before and after ZVI column was nearly same. It means that breakdown of azo bond by ZVI reducing power could increase biodegradability of synthetic dye wastewater.

• Archives of Pharmacal Research
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• 제25권5호
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• pp.613-616
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• 2002

A high-performance liquid chromatographic method has been developed for the separation and quantification of chrysin and synthetic chrysin derivatives (12 chrysin alkyl and 7 chrysin acyl derivatives). The chromatography was performed using a $Nova-Pak^{\circledR}C_{18}$ column. A RP-HPLC was performed by using a binary mixture (MeOH-10 mM H$_3$PO$_4$) as a mobile phase, and the column temperature was maintained at room temperature. A flow rate was 1.0 ml/min, and the effluent was monitored at a wavelenth of 280 nm. The retention times for chrysin acyl and alkyl derivatives were within 10 minutes and 20 minutes, respectively. The absolute recovery of samples were all over 96%. The detection limits were 0.1~18 ng at S/N = 3 ratio.

• 한국안전학회지
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• 제13권4호
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• pp.49-58
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• 1998

On the stability of the Timoshenko cantilever column subjected of a compressive follower force, the influences of the moment of inertia of the tip mass at the free end and the characteristics of a translational spring at the free end of the column are studied. The equations of motion and boundary conditions of system are estabilished by using the d'Alembert virtual work of principle. On the evaluation of stability of the column, the effect of the shear deformation and rotatory inertia is considered in calculation. The moment of inertia of the tip mass at the free end of the column is changed by adjusting the distance c, from the free end of the column to the tip mass center. The free end of the column is supported elastically by a translational spring. For the maintenance of the good stability of the column, it is also proved that the constant of the translational spring at the free end must be very large for the case without a tip mass while it must be small for the case with a tip mass. Therefore, it is found that the shape of the tip mass and the characteristic of the spring at the free end are very effective elements for the stability of the column when the columns subjected to a compressive follower force are designed.

• 한국축산식품학회지
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• 제40권1호
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• pp.87-95
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• 2020

In this study, we separated and purified lipase inhibitory peptide from fermented milk by Lactobacillus plantarum Q180 with the aim of developing a new functional anti-lipase activity yogurt product. L. plantarum 180 was inoculated into 10% reconstituted skimmed milk and incubated at 37℃ until the pH of the culture reached pH 4.4. The lipase activity was measured using porcine pancreatic lipase. The lipase inhibitory peptides were gradually isolated by ultrafiltration, reversed phase column chromatography (RPC), reversed phase high-performance liquid chromatography (RP-HPLC), and gel permeation high-performance liquid chromatography (GP-HPLC) from the fermented milk by L. plantarum Q180. An ODS-AQ column was used for the RPC, a Vydac C18 column for the RP-HPLC, and a Superdex Peptide HR column for the GP-HPLC. The peptide was composed of Asp, Thr, Ile, Ser, Ala, and Gln, and the anti-lipase activity (IC₅₀) was 2,817 ㎍/mL.

• 한국콘텐츠학회논문지
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• 제10권8호
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• pp.18-25
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• 2010

본 논문에서는 열방향 2차원 PCA(Column-directional 2 Dimensional PCA, C2DPCA) 와 행방향 2차원 LDA(Row-directional 2 Dimensional LDA, R2DLDA)를 사용하여 얻은 각각의 투영 행렬을 동시에 사용하는 방법을 제안하였다. 제안 방법은 얼굴의 가로 특징과 세로 특징을 모두 포함한 저 차원의 특징 행렬을 얻음으로써, 훈련 영상의 수에 관계없이 안정적이고 높은 인식률을 얻을 수 있다. 또한, 같은 알고리즘으로 가로 방향과 세로 방향에 PCA와 LDA를 각각 달리 적용한 실험(C2DPCA & R2DLDA, C2DLDA & R2DPCA)에서 가로 방향의 특징에 2차원 LDA를 적용한 시스템(C2DPCA & R2DLDA)이 그 반대의 경우보다 저차원으로 높은 인식률을 얻을 수 있음을 확인할 수 있었다. 실험 결과 제안한 방법이 2DPCA와 2DLDA 등 의 기존 방법보다 인식율이 높은 99.4%를 얻었다. 또한 제안 방법의 인식 처리속도도 기존의 2DPCA와 2DLDA 방법보다 3배 이상 빠름을 확인하였다.

• 한국잠사곤충학회지
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• 제45권2호
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• pp.90-95
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• 2003

최근 천연색소의 다양한 생리 활성이 보고됨에 따라 오디에 함유된 C3G 색소에 대해서도 관심이 높아지고 있다. 이에 오디로부터 C3G를 분리$.$정제하고 정제된 C3G와 표준물질의 분자량을 비교ㆍ동정하였다. 또한 유전자원으로 보존되고 있는 뽕 품종 및 계통에 대한 C3G 함량을 분석하여 고함유 계통을 선발하였다. 1. Amberlite IRC-50 ion exchange resin이 충진된 유리 column을 산성화시킨 후 이온교환수로 비흡착색소, 수용성 당류, 유기산 및 아미노산 등을 제거하였으며, column에 흡착된 색소를 용출 및 감압농축하여 분말상태로 정 제된 색소물질 C3G(cyanidin-3-glucoside)를 얻었다. 2. Waters 996 Photodiods Array Detector를 이용하여 정제된 C3G(cyanidin-3-glucoside)의 흡수 spectrum을 조사한 결과, 각각 516nm과 280nm에서 최대흡수 spectrum을 보임으로써 anthocyanin계 색소의 특이적 흡수 파장 영역인 500-550nm(Band I)과 280nm전후(Band II)와 일치하여 open column에 의해 분리된 색소가 anthocyanin 계열의 색소임을 확인하였다. 3. LC-Mass(Waters 2690 alliance Separation Module)를 사용하여 분자량을 확인한 결과, 449로서 C3G 표준물질의 분자량과 일치하였다. 4. 유전자원으로 보존되고 있는 뽕 품종 및 계통에 대한 C3G 함량을 분석한 결과, 공시한 35계통의 C3G 평균 함량은 0.9%였으며 계통간 함량의 차이가 있는 것으로 나타났다. 5. '수성뽕'. '강선' 및 '절곡조생(충북)'은 C3G함량은 물론 수량, 과중 및 당도에 대한 과실적 특성조사에서 양호한 성적을 나타내어 오디생산용 유망 계통으로 선발하였다.

• Bulletin of the Korean Chemical Society
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• 제4권4호
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• pp.183-188
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• 1983

Two major and two minor polyacetylenes were isolated from fresh white Korean ginseng roots. The petroleum ether-ethyl ether fractions containing the polyacetylene compounds were collected through solvent fractionation, partition and silica gel column chromatography. Further separation of polyacetylenic fractions was proceeded by bonded normal phase HPLC utilizing a moderately nonpolar microparticulate column. The low pressure liquid chromatography was used for the semi-preparative separation. The chemical structures of the two major polyacetylenes separated were determined by UV, IR, $^1H$ NMR, $^{13}C$ NMR, mass spectra and elemental analysis. One of them is identified to be heptadeca-1-en-4, 6-diyne-3, 9, 10-triol, a new structure, and the other is heptadeca-1, 9-dien-4, 6-diyn-3-ol, known as panaxynol.

• 한국지진공학회논문집
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• 제17권4호
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• pp.159-169
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• 2013

The purpose of this study is to investigate the inelastic behavior of hollow reinforced concrete bridge column sections with reinforcement details for material quantity reduction and to provide the details and reference data. Among the numerous parameters, this study concentrates on the shape of the section, the reinforcement details, the diameter of the transverse reinforcement and loading types. Eighteen column section specimens were tested under quasi-static monotonic loading. In this study, the computer program RCAHEST (Reinforced Concrete Analysis in Higher Evaluation System Technology) was used. A modified lateral confining effect model was adopted for the hollow bridge column sections. This study documents the testing of hollow reinforced concrete bridge column sections with reinforcement details for material quantity reduction and presents conclusions based on the experimental and analytical findings.

• 복합신소재구조학회 논문집
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• 제3권4호
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• pp.27-37
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• 2012

The construction of a moment connection for a rectangular hollow section (RHS) column and a H-shaped beam is difficult because the RHS is a closed section. When a inner diaphragm is used for such a connection, in general, it is installed after cutting the HSS columns, which results in increased construction work. This paper suggests a new fabrication method to overcome such problems: An inner diaphragm is welded to inside a C-shaped section first, and then a column is fabricated by welding two C-shaped sections. This fabrication method is superior to a classic method in terms of constructibility. An experimental and a numerical study using Ansys 9.0 were performed in order to compare the strength of connections with respect to the presence of concrete, the corner shape of diaphragm, and the axis of loading. The experimental results including initial stiffness and ultimate loads are reported and the analytical results including load transfer mechanism, degree of stress concentration, and strain distribution are also reported.