• 한국동물학회:뉴스레터
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• 제14권1호
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• pp.16-21
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• 1997

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 공동 춘계학술대회
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• pp.77.2-77
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• 1999

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
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• pp.11-11
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• 2003

No Abstract, See Full Text

• 미생물학회지
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• 제50권2호
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• pp.152-157
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• 2014

이 논문은 Callyspongia elegans에서 서식하는 세균군집에 관한 내용이다. 해양세균은 marine agar를 사용하여 해면동물 C. elelgans에서 분리하였다. 그 결과 112균주를 분리하였으며, 본 연구에 사용하였다. 현미경 및 그람 염색을 통해 형태학적 표현형질을 측정하였다. 분리균주의 집락 색소는 노란색, 갈색, 아이보리색, 흰색으로 나타났다. 그람염색 결과 37균주는 그람양성균이였으며, 75균주는 그람 음성균이었다. 균주의 형태는 분리균주 중 79균주는 구균형태로 관찰되었고, 16균주는 간균이었다. 16S rDNA 유전자 염기서열 분석을 통해 분리균주들의 계통학적 특성을 파악하였다. 그 결과, 분리된 112균주는 5개의 주요 계통군이 확인되었으며, Alphaproteobacteria는 39%, Gammaproteobacteria는 22%, Acinobacteria는 14%, Firmicutes는 9%, Bacteroidetes는 6%에 속하는 것으로 나타났다. 그리고 16S rDNA 유전자 염기서열을 통해 계통분석 결과 15균주가 새로운 속 또는 종으로 분류될 가능성을 나타났으며, 앞으로 추가적인 실험이 필요한 실정이다.

• Environmental Analysis Health and Toxicology
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• 제30권
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• pp.7.1-7.7
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• 2015

Objectives The widely promising applications of graphene nanomaterials raise considerable concerns regarding their environmental and human health risk assessment. The aim of the current study was to evaluate the toxicity profiling of graphene family nanano-materials (GFNs) in alternative in vitro and in vivo toxicity testing models. Methods The GFNs used in this study are graphene nanoplatelets ([GNPs]-pristine, carboxylate [COOH] and amide [$NH_2$]) and graphene oxides (single layer [SLGO] and few layers [FLGO]). The human bronchial epithelial cells (Beas2B cells) as in vitro system and the nematode Caenorhabditis elegans as in vivo system were used to profile the toxicity response of GFNs. Cytotoxicity assays, colony formation assay for cellular toxicity and reproduction potentiality in C. elegans were used as end points to evaluate the GFNs' toxicity. Results In general, GNPs exhibited higher toxicity than GOs in Beas2B cells, and among the GNPs the order of toxicity was pristine > $NH_2$ > COOH. Although the order of toxicity of the GNPs was maintained in C. elegans reproductive toxicity, but GOs were found to be more toxic in the worms than GNPs. In both systems, SLGO exhibited profoundly greater dose dependency than FLGO. The possible reason of their differential toxicity lay in their distinctive physicochemical characteristics and agglomeration behavior in the exposure media. Conclusions The present study revealed that the toxicity of GFNs is dependent on the graphene nanomaterial's physical forms, surface functionalizations, number of layers, dose, time of exposure and obviously, on the alternative model systems used for toxicity assessment.

• Molecules and Cells
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• 제38권3호
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• pp.236-242
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• 2015

Intake of caffeine during pregnancy can cause retardation of fetal development. Although the significant influence of caffeine on animal development is widely recognized, much remains unknown about its mode of action because of its pleiotropic effects on living organisms. In the present study, by using Caenorhabditis elegans as a model organism, the effects of caffeine on development were examined. Brood size, embryonic lethality, and percent larval development were investigated, and caffeine was found to inhibit the development of C. elegans at most of the stages in a dosage-dependent fashion. Upon treatment with 30 mM caffeine, the majority ($86.1{\pm}3.4%$) of the L1 larvae were irreversibly arrested without further development. In contrast, many of the late-stage larvae survived and grew to adults when exposed to the same 30 mM caffeine. These results suggest that early-stage larvae are more susceptible to caffeine than later-stage larvae. To understand the metabolic responses to caffeine treatment, the levels of expression of cytochrome P450 (cyp) genes were examined with or without caffeine treatment using comparative microarray, and it was found that the expression of 24 cyp genes was increased by more than 2-fold (p < 0.05). Among them, induction of the cyp-35A gene family was the most prominent. Interestingly, depletion of the cyp-35A family genes one-by-one or in combination through RNA interference resulted in partial rescue from early larval developmental arrest caused by caffeine treatment, suggesting that the high-level induction of cyp-35A family genes can be fatal to the development of early-stage larvae.

• 생약학회지
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• 제49권1호
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• pp.40-46
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• 2018

Chaenomeles sinensis (Thouin) Koehne fruit (Rosaceae) has been used as a traditional medicine in Korea, Japan and China to treat sore throat, diarrhea and inflammation. The ethanol extract of C. sinensis fruit was successively partitioned as methylene chloride, ethyl acetate, n-butanol and $H_2O$ soluble fractions. Among those fractions, the n-butanol fraction showed the most potent DPPH radical scavenging and superoxide quenching activities. To verify antioxidant activities, the n-butanol fraction was checked the activities of superoxide dismutase (SOD) and catalase activities, and intracellular ROS levels and oxidative stress tolerance in Caenorhabditis elegans. Furthermore, to see if increased stress tolerance of worms by treating of the n-butanol fraction was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain. Consequently, the n-butanol fraction elevated SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose-dependent manner. Moreover, the n-butanol fraction-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.

• 생약학회지
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• 제47권3호
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• pp.280-286
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• 2016

Helianthus tuberosus Linne (Compositae) has been widely used as a folk remedy to treat various ailments including fever, bleeding, fracture and contusion. This study was designed to elucidate the lifespan extending activities MeOH extract of the tubers of Helianthus tuberosus Linne (MHT) using Caenorhabditis elegans (C. elegans) model system. In the current study, we found that the lifespan of worms was significantly extended by MHT supplement, dose-dependently. MHT also provided robust protection against various stress environments such as osmotic, thermal and oxidative condition. In addition, elevated antioxidant enzyme activities by MHT resulted in attenuation of intracellular reactive oxygen spices (ROS) levels, suggesting antioxidant capacity of MHT might be associated with longevity properties. Herein, we showed that altered food intake and growth of worms were also involved in the MHT activity. Furthermore, MHT increased body movement in aged worms, indicating possible role for MHT in healthspan.

• Journal of Microbiology and Biotechnology
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• 제30권11호
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• pp.1626-1639
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• 2020

In Caenorhabditis elegans, SHN-1 is the homologue of SHANK, a scaffolding protein. In this study, we determined the molecular basis for SHN-1/SHANK in the regulation of innate immune response to fungal infection. Mutation of shn-1 increased the susceptibility to Candida albicans infection and suppressed the innate immune response. After C. albicans infection for 6, 12, or 24 h, both transcriptional expression of shn-1 and SHN-1::GFP expression were increased, implying that the activated SHN-1 may mediate a protection mechanism for C. elegans against the adverse effects from fungal infection. SHN-1 acted in both the neurons and the intestine to regulate the innate immune response to fungal infection. In the neurons, GLR-1, an AMPA ionotropic glutamate receptor, was identified as the downstream target in the regulation of innate immune response to fungal infection. GLR-1 further positively affected the function of SER-7-mediated serotonin signaling and antagonized the function of DAT-1-mediated dopamine signaling in the regulation of innate immune response to fungal infection. Our study suggests the novel function of SHN-1/SHANK in the regulation of innate immune response to fungal infection. Moreover, our results also denote the crucial role of neurotransmitter signals in mediating the function of SHN-1/SHANK in regulating innate immune response to fungal infection.

• 한국정보통신학회논문지
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• 제25권7호
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• pp.903-909
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• 2021

예쁜꼬마선충(Caenorhabditis elegans)은 염기서열이 완전히 밝혀진 동물로 유전자 기능 분석, 동물 행동 연구 등 다양한 연구 분야에 사용되는 대표적인 생물 종이다. 그동안 선충을 이용해 물의 오염 여부를 판별하기 위한 바이오 모니터링 시스템에 대한 여러 연구들이 있었다. 본 논문은 하천의 수질 오염의 원인이 되는 화학물질을 식별하기 위해 선충의 수영 행동이 활용 가능한 지를 보여주기 위해 기계학습 기반의 바이오 모니터링 시스템을 제안한다. 선충의 수영 행동을 대표하기 위해 선충을 대상으로 가지 길이 유사성(Branch Length Similarity) 엔트로피를 계산한다. 그리고 BLS 엔트로피의 조합인 BLS 엔트로피 프로파일을 클러스터링 알고리즘을 사용해 몇 가지 패턴으로 유형화하여 데이터 집합을 만든다. 0.1ppm 농도의 포름알데히드, 벤젠, 톨루엔이 첨가된 아레나에서 선충의 수영 행동을 촬영하고 개발한 히든 마코프 모델(Hidden Markov Model: HMM)의 성능을 검증한다.