Browse > Article
http://dx.doi.org/10.7845/kjm.2014.4030

Phylogenetic Diversity of Bacterial Community Inhabited in Callyspongia elegans  

Park, So-Hyun (Department of Aquatic Life Medicine, College of Ocean Science)
Kim, Ji-Young (Marine and Environmental Research Institute, Jeju National University)
Kim, Young-Ju (Research Institute for Basic Science, Jeju National University)
Heo, Moon-Soo (Department of Aquatic Life Medicine, College of Ocean Science)
Publication Information
Korean Journal of Microbiology / v.50, no.2, 2014 , pp. 152-157 More about this Journal
Abstract
The aim of this study was to investigate the bacterial community inhabited in Callyspongia elegans. Marine bacteria were isolated from the marine sponge C. elegans using marine agar. The resulting 112 isolated pure cultures were then used for further study. They were characterized by determining morphological characteristics through Gram's staining and morphological observation. The colony pigments of bacterial isolates were characterized as yellow, brown, ivory, and white. Thirty-seven strains were found to be Gram-positive and 75 strains were Gram-negative. Seventy-nine strains were coccus-shaped, while 16 strains were rod-shaped. On the basis of the results of the comparative analyses of 16S rDNA gene sequences, the 112 isolated bacteria were divided into 5 major groups: Alphaproteobacteria (39%), Gammaproteobacteria (22%), Actinobacteria (14%), Fimicutes (9%), and Bacteroidetes (6%). It is strongly suggested that fifteen isolates are candidates for a new genera or species, based on the analyses of 16S rDNA gene sequences.
Keywords
16S rDNA; Callyspongia elegans; bacterial community; marine sponge; phylogenetic tree;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Cho, H.H., Shim, E.J., and Park, J.S. 2010. Phylogenetic diversity of bacteria associated with the marine sponges, Spirastrella abata and Cinachyrella sp. Kor. J. Microbiol. 46, 177-182.   과학기술학회마을
2 Eilers, H., Pernthaler, J., Glockner, F.O., and Aman, R. 2000. Culturability and in situ abundance of pelagic bacteria from the North Sea. Appl. Environ. Microbiol. 66, 3044-3051.   DOI   ScienceOn
3 Felsenstein, J. 1981. Evolutionary trees from DNA sequences: a maximum like lihood approach. J. Mol. Evol. 17, 368-376.   DOI   ScienceOn
4 Friedrich, A.B., Hacker, J., Fischer, I., Proksch, P., and Hentschel, U. 2001. Temporal variations of the microbial community associated with the Mediterranean sponge Aplysina aerophoba. FEMS Microbiol. Ecol. 38, 105-113.   DOI
5 Guangyi, W. 2006. Diversity and biotechnological potential of the sponge-associated microbial consortia. J. Ind. Microbiol. Biotechnol. 33, 545-551.   DOI   ScienceOn
6 Hentschel, U., Usher, K.M., and Taylor, M.W. 2006. Marine sponges as microbial fermenters. FEMS Microbiol. Ecol. 55, 167-177.   DOI   ScienceOn
7 Kennedy, J., Baker, P., Piper, C., Cotter, P.D., Walsh, M., Mooij, M.J., Bourke, M.B., Rea, M.C., O'Connor, P.M., Ross, R.P., and et al. 2009. Isolation and analysis of bacteria with antimicrobial activities from the marine sponge Haliclona simulans collected from Irish Waters. Mar. Biotechnol. 11, 384-396.   DOI   ScienceOn
8 Lafi, F.F., Garson, M.J., and Fuerst, J.A. 2005. Culturable bacterial symbionts isolated from two distinct sponge species (Pseudoceratina clavata and Rhabdastrella globostellata) from the great barrier reef display similar phylogenetic diversity. Microb. Ecol. 50, 213-220.   DOI   ScienceOn
9 Braekman, J. and Daloze, D. 2004, Chemical and biological aspects of sponge secondary metabolites. Phytochem. Rev. 3, 275-282.   DOI   ScienceOn
10 Cho, H.H. and Park, J.S. 2009. Comparative analysis of the community of culturable bacteria associated with sponges, Spirastrella abata and Spirastrella panis by 16S rDNA-RFLP. Kor. J. Microbiol. 45, 155-162.   과학기술학회마을
11 Sung, H.R. and Ghim, S.Y. 2010. Bacterial diversity and distribution of cultivable bacteria isolated from Dokdo Island. Kor. J. Microbiol. Biotechnol. 38, 263-272.   과학기술학회마을
12 Thiel, V., Leininger, S., Schmaljohann, R., Brummer, F., and Imhoff, J.F. 2007. Sponge- specific bacterial associations of the Mediterranean sponge Chondrilla nucula (Demospongiae, Tetractinomorpha). Microb. Ecol. 54, 101-111.   DOI   ScienceOn
13 Tamaki, H., Sekiguchi, Y., Hanada, S., Nakamura, K., Nomura, N., Matsumura, M., and Kamagata, Y. 2005. Comparative analysis of bacterial diversity in freshwater sediment of a shallow eutrophic lake by molecular and improved cultivation-based techniques. Appl. Environ. Microbiol. 71, 2162-2169.   DOI   ScienceOn
14 Tamura, K., Dudley, J., Nei, M., and Kumar, S. 2007. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596-1599.   DOI   ScienceOn
15 Taylor, M.W., Schupp, P.J., de Nys, R., Kjelleberg, S., and Steinberg, P.D. 2005. Biogeography of bacteria associated with the marine sponge Cymbastela concentrica. Environ. Microbiol. 7, 419-433.   DOI   ScienceOn
16 Thompson, J.D., Higgins, D.G., and Gibson, T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680.   DOI   ScienceOn
17 Thomas, T.R.A., Kavlekar, D.P., and LokaBharathi, P.A. 2010. Marine drugs from sponge-microbe association-a review. Mar. Drugs 8, 1417-1468.   DOI   ScienceOn
18 Thoms, C., Horn, M., Wagner, W., Hentschel, U., and Proksch, P. 2003. Monitoring microbial diversity and natural products profiles of the sponge Aplysina cavernicola following trasplantation. Mar. Biol. 142, 685-692.   DOI
19 Muscholl-Silberhorn, A., Thiel, V., and Imhoff, J.F. 2008. Abundance and bioactivity of cultured sponge-associated bacteria from the Mediterranean Sea. Microbiol. Ecol. 55, 94-106.   DOI   ScienceOn
20 Mohamed, N.M., Rao, V., Hamann, M.T., Kelly, M., and Hill, R.T. 2008. Monitoring bacterial diversity of the marine sponge Ircinia strobilina upon transfer into aquaculture. Appl. Environ. Microbiol. 74, 4133-4143.   DOI   ScienceOn
21 Park, J.S. 2010. Bacterial community diversity associated with two marine sponges from the South Pacific Ocean based on 16S rDNA-DGGE analysis. Kor. J. Microbiol. 46, 255-260.   과학기술학회마을
22 Park, J.S., Sim, J.J., and An, K.D. 2009. Community structure of bacteria associated with two marine sponges from Juju Island based on 16S rDNA-DGGE profile. Kor. J. Microbiol. 45, 170-176.   과학기술학회마을
23 Radwan, M., Hanora, A., Zan, J., Mohamed, N.M., Abo Elmatty, D.M., Abou-El-Ela, S.H., and Hill, R.T. 2010. Bacterial community analyses of two red sea sponges. Mar. Biotechnol. 12, 350-360.   DOI   ScienceOn
24 Lee, O.O., Wong, Y.H., and Qian, P.Y. 2009. Inter and intraspecific variations of bacterial communities associated with marine sponges from San Juan Island, Washington. Appl. Environ. Microbiol. 75, 3513-3521.   DOI   ScienceOn
25 Levina, E.V., Kalinovsky, A.I., Andriyashenko, P.V., Dmitrenok, P.S., Aminin, D.L., and Stonik, V.A. 2005. Phrygiasterol, a cytotoxic cyclopropane containing polyhydroxysteroid, and related compounds from the pacific starfish Hippasteria phrygiana. J. Nat. Prod. 68, 1541-1544.   DOI   ScienceOn
26 Alfreider, A., Pernthhaler, J., Amann, R., Sattler, B., Glockner, F.O., Wille, A., and Psenner, R. 1996. Community analysis of the bacterial assemblages in the winter cover and pelagic layers of high mountain lake by in situ hybridization. Appl. Environ. Microbiol. 62, 2138-2144.
27 Li, Z.Y., He, L.M., Wu, J., and Jiang, Q. 2006. Bacterial community diversity associated with four marine sponges from the South China Sea based on 16S rDNA-DGGE fingerprinting. J. Exp. Mar. Biol. Ecol. 329, 75-85.   DOI   ScienceOn
28 Saitou, N. and Nei, M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
29 Graeber, I., Kaesler, I., Borchert, M.S., Dieckmann, R., Page, T., Lurz, R., Nielsen, P., Dohren, H.V., Michaelis, W., and Szewzyk, U. 2008. Spongiibacter marinus gen. nov., sp. nov., a Halophilic marine bacterium isolated from the boreal sponge Haliclona sp.1. Int. J. Syst. Evol. Microbiol. 58, 585-590.   DOI   ScienceOn