• Toxicological Research
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• 제30권1호
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• pp.45-48
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• 2014

Helicobacter pylori (H. pylori) is the most important factor of gastric disease in clinical practice. Moreover, smoking, stress and a poor diet may be additive factors for gastric damage. With these factors, increasing infection of H. pylori triggers gastritis, gastric ulcers and gastric cancer. To develop a new protective agent, we are concerned with plant-derived extract. The extract of Coptis chinensis (C. chinensis) and its constituents were investigated to assess their protective activities against gastric damage. The C. chinensis extract showed a scavenging effect against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radicals, inhibition of H. pylori colonization and antiulcerogenic activities in rat. In particular, palmatine derived from C. chinensis was found to be the novel protective agent. It is better than the C. chinensis extract, berberine, a well-known constituent of C. chinensis. We suggest that palmatine from the root cortex of C. chinensis may be a good candidate for the development of new pharmaceuticals to prevent gastric disease.

• 미생물학회지
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• 제49권1호
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• pp.17-23
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• 2013

Candida 바이오필름은 숙주조직이나 인체에 삽입된 의료기구의 표면에 자라는 진균의 군락으로 전통적인 항진균제에 대한 내성을 유발한다. 황련(Coptidis chinensis)의 뿌리는 극동지방에서 의료용 목적으로 널리 사용되어 왔다. 본 연구의 목적은 임상에서 분리한 C. albicans 바이오필름 형성 균주가 형성한 바이오필름에 대한 C. chinensis 수용성 추출물의 효과와 C. albicans 바이오필름 형성을 저해하는 데 기여하는 항진균활성을 평가하는 데 있다. 바이오필름에 대한 효과는 XTT [2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide)] 환원분석법을 사용하였으며, 조사된 모든 균주에 대한 대사활성은 $98{\mu}g/ml$의 C. chinensis 수용성 추출물에 의해 현저하게 감소($57.3{\pm}14.7%$)되어 유의성 있는 항바이오필름 활성을 나타내었다. Fluorescein diacetate와 propidium iodide로 이중 염색한 결과 C. chinensis 추출물은 C. albicans의 세포막을 손상시켰다. C. chinensis 수용성 추출물은 살진균 활성을 나타냈고, C. albicans 바이오필름의 폴리스티렌 표면으로의 부착을 억제하였으며 C. albicans를 $G_o/G_1$기에 머무르게 하여 바이오필름이나 출아법에 의한 증식을 억제시켰다. 본 연구의 결과는 C. chinensis 추출물이 목표가 되는 C. albicans에 복합적으로 유해한 효과를 내어 궁극적으로는 C. albicans 바이오필름 형성을 억제함을 나타낸다. 따라서 C. chinensis 추출물은 바이오필름과 관련된 캔디다의 감염을 치료하고 제거하기 위한 천연물 기반항진균제 개발에 대한 높은 가능성을 가진다.

• 대한본초학회지
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• 제25권3호
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• pp.111-116
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• 2010

Objectives : This experimental study was performed to investigate the antibacterial effect of Caesalpinia sappan and Coptis chinensis extract against B. cereus and V. parahaemolyticus. Methods : Methanol extracts of C. sappan and C. chinensis was tested against B. cereus and V. parahaemolyticus by paper disc method. Results : The growth of B. cereus and V. parahaemolyticus was inhibited by C. sappan and C. chinensis extract among 6 kinds of medicinal plant extracts. The extract of C. sappan and C. chinensis extract inhibited the growth of V. parahaemolyticus and B. cereus, respectively. The growth of B. cereus and V. parahaemolyticus had a tendency to increase depend on the concentration of the extract. EtOEt and EtOAc fractions and EtOEt and BuOH fractions of the C. sappan extract had a high antibacterial activity against B. cereus and V. parahaemolyticus, respectively. And, BuOH and $H_2O$ fractions of the C. chinensis extract showed antibacterial activity against B. cereus highly. Conclusions : C. sappan and C. chinensis extract efficiently inhibited the growth of B. cereus and V. parahaemolyticus.

• International Journal of Oral Biology
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• 제45권4호
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• pp.143-151
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• 2020

Streptococcus mutans and Streptococcus sobrinus play important roles in dental caries. Coptis chinensis is a natural product with antimicrobial activity against enterobacteria; however, its effects on oral streptococci are still unknown. Therefore, the effects of C. chinensis on the growth and biofilm formation of the representative cariogenic bacteria S. mutans and S. sobrinus were investigated for the possible use of C. chinensis as an anticaries agent. The C. chinensis extract was diluted with sterile distilled water, and 0.1-2.5% of the extract was used in the experiment. The effects of the C. chinensis extract on the growth and glucan formation of S. mutans and S. sobrinus were measured by viable cell counting and spectrophotometry at 650 nm absorbance, respectively. Crystal violet staining was also carried out to confirm the C. chinensis extract's inhibitory effect on biofilm formation. The C. chinensis extract significantly inhibited the growth of S. mutans and S. sobrinus at concentrations of ≥ 0.3% as compared with the control group. The viable cell count of colonies decreased by 1.7-fold and 1.2-fold at 2.5% and 1.25%, respectively, compared with the control group. The biofilm formation of S. mutans and S. sobrinus was inhibited by > 20-fold at C. chinensis extract concentrations of ≥ 1.25% as compared with the control group. In summary, the C. chinensis extract inhibited the growth and biofilm and glucan formation of S. mutans and S. sobrinus. Therefore, C. chinensis might be a potential candidate for controlling dental caries.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.291-312
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• 2003

Reactive oxygen species are capable of damaging biomolecules such as lipids, proteins, and DNA, which can not only lead to various diseases, but also oxidative damage resulting aging. In our previous study, Cercis chinensis (Leguminosae) showed a potent antioxidant activity. Nineteen compounds were isolated through antioxidant activity-guided fractionation. The C. chinensis extract and some of the constituents exhibited a potent antioxidant activity on the free radicals and lipid peroxidation and a notable protective effect on the t-BuOOH induced oxidative damage. In vivo test of skin damage induced by UVB irradiation, the extract of C. chinensis and a constituent, piceatannol, exhibited a significant protective effect. The life-span of the HEK-N/F cells were extended by 1.21-2.12 fold as a result of the continuous administration of 3 $\mu\textrm{g}$/ml of the C. chinensis extract and the active constituents compared to that of the control. These observations were attributed to the inhibitory effect of the C. chinensis extract and its constituents on the age-dependent shortening of the telomere. Thus, C. chinensis was demonstrated to protect the skin cells against oxidative stress and inhibit thereby the cellular aging, followed by expectation as anti-aging cosmetic ingredient.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.117-138
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• 2003

Reactive oxygen species are capable of damaging biomolecules such as lipids, proteins, and DNA, which can not only lead to various diseases, but also oxidative damage resulting aging. In our previous study, Cercis chinensis (Leguminosae) showed a potent antioxidant activity. Nineteen compounds were isolated through antioxidant activity-guided fractionation. The C. chinensis extract and some of the constituents exhibited a potent antioxidant activity on the free radicals and lipid peroxidation and a notable protective effect on the t-BuOOH induced oxidative damage. In vivo test of skin damage induced by UVB irradiation, the extract of C. chinensis and a constituent, piceatannol, exhibited a significant protective effect. The life-span of the HEK-N/F cells were extended by 1.21-2.12 fold as a result of the continuous administration of 3 $\mu\textrm{g}$/ml of the C. chinensis extract and the active constituents compared to that of the control. These observations were attributed to the inhibitory effect of the C. chinensis extract and its constituents on the age-dependent shortening of the telomere. Thus, C. chinensis was demonstrated to protect the skin cells against oxidative stress and inhibit thereby the cellular aging, followed by expectation as antiaging cosmetic ingredient.

• Natural Product Sciences
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• 제25권4호
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• pp.334-340
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• 2019

Cuscuta chinensis Lam. and Cuscuta japonica Choisy are parasitic plants. C. chinensis seeds were traditionally used for treatment of kidney and liver deficiencies. C. japonica seeds were used as tonic medicine to improve liver function and strengthen kidneys, treatment of high blood pressure, chronic diarrhea, and sore eyes. Cuscutae Semen are seeds of only C. chinensis in Korean Herbal Pharmacopoeia (K.H.P.). The developed HPLC-PDA method easily, accurately, and sensitively quantified using eight marker compounds [hyperoside (1), astragalin, (2), quercetin (3), kaempferol (4), chlorogenic acid (5), 3,4-di-O-caffeoylquinic acid (6), 1,5-di-O-caffeoylquinic acid (7), and 4,5-di-O-caffeoylquinic acid (8)]. In addition, the method may be used to distinguish seeds between C. chinensis Lam. and C. japonica Choisy. Furthermore, the result from the current study was applied to clarify samples between steam processed and unprocessed samples of C. chinensis by pattern analysis.

• Biomolecules & Therapeutics
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• 제3권2호
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• pp.111-115
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• 1995

Natural products, total number of 175, were screened to test for their effect on the replication of human immunodeficiency virus type 1 (HIV-1). Five of them, such as Eriobotrya japonica, Eugenia caryphyllata, Cuscuta chinensis, Glycyrrhiza uralensis, and Coptis chinensis were shown to be effective in inhibiting the replication of HIV-1 in tissue culture and their selectivity indexes were 42, 40, 14, 18 and 65, respectively. To further fractionate Coptis chinensis, which is shown to be highest anti-HIV-1 activity, methanol extracts of Coptis chinensis were fractionated into methylene chloride at pH3, pH10 and water residue. The selectivity Indexes of CH$_2$C1$_2$(pH 3), CH$_2$C1$_2$(pH 10) and water residue were 50, 22 and 98 respectively. Our results show that the water residue of Coptis chinensis was the most effective for anti-HIV-1 activity.

• 한국미생물·생명공학회지
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• 제31권1호
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• pp.69-74
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• 2003

천연 식물 재료를 이용한 여드름 개선제 개발을 위해 솔잎, 오미자, 에탄올 추출물의 여드름 원인균으로 알려진 Propionibacterium acnes, Staphylococcus epidermidis와 Malasseizia furfur에 대한 증식억제 효과를 검토한 결과 여드름 원인균 3종류 모두에 항균력을 나타내었다. 오미자. 솔잎의 M. furfur에 대한 성장억제효과는 초기배양시 균의 억제효과가 뚜렷하게 나타났으며, 오미자의 경우 모든 농도에서 $10^1$CFU/mL 이하 또는 사멸하는 경향을 나타내었다. S. epidermidis에 대한 오미자, 솔잎이 높은 성장억제효과를 보였으며, 0.12% 이상일 때 강한 항균효과를 나타내었다. P. acnes의 성장은 솔잎, 오미자를 0.06% 이상 첨가한 경우 배양 1일째 모두 사멸하여 매우 높은 항균활성을 나타내었다. 솔잎, 오미자의 생육억제효과는 여드름 원인균 중 P. acnes가 가장 강하게 나타났고, M. furfur, S. epidermidis 순이었다. 각 천연물의 여드름 원인균에 대한 최소저해 농도를 측정한 결과, P. acnes의 MIC는 0.075 mg/mL로 가장 낮게 나타났고, M. furfur는 0.6-l.8 mg/mL, S. epidermidis에 대해 1.2%-l.8mg/mL으로 높게 나타났다.$ 80^{\circ}C$, $100^{\circ}C$에서 30분 동안, 121$^{\circ}C$ 15분 열처리한 오미자와 솔잎 추출물의 여드름 원인균에 대한 항균효과는 가열 온도가 증가함에 따라 다소 감소하였으나 추출물의 항균활성에는 뚜렷한 영향을 미치지 않았다.

• Journal of Applied Biological Chemistry
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• 제59권1호
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• pp.5-7
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• 2016

딱지꽃(Potentilla chinensis)은 약초식물의 하나로 항염, 지혈, 해독 그리고 해열 등의 효과가 알려져 있다. 특히 딱지꽃의 뿌리는 약제로써 중요한 가치를 지니고 있다. 그러나 기존에 딱지꽃의 줄기나 잎에 대한 유효성분에 대한 연구가 시도된 반면 딱지꽃의 뿌리의 주요성분을 분석한 사례가 없었다. 따라서 본 연구는 딱지꽃의 뿌리를 다양한 용매별로 추출하고 주요한 물질을 분리정제 하였다. 분리 정제된 물질을 NMR과 mass 분석을 통하여 물질을 동정하였고 그 결과 주요성분이 화합물 (1)임을 확인하였다. 또한, 동정된 물질의 산업적 응용을 위해 딱지꽃 뿌리에서 용매별로 화합물 (1) 추출양을 정량하였다.