Inhibition of HIV-1 Replication by Extract of Coptis chinensis

황련추출물에 의한 HIV-1 복제 저해

  • 송만기 (포항공과대학교 생명과학과) ;
  • 이안휘 (포항공과대학교 생명과학과) ;
  • 김영호 (KIST 유전공학연구소) ;
  • 이정준 (KIST 유전공학연구소) ;
  • 성영철 (포항공과대학교 생명과학과)
  • Published : 1995.06.01

Abstract

Natural products, total number of 175, were screened to test for their effect on the replication of human immunodeficiency virus type 1 (HIV-1). Five of them, such as Eriobotrya japonica, Eugenia caryphyllata, Cuscuta chinensis, Glycyrrhiza uralensis, and Coptis chinensis were shown to be effective in inhibiting the replication of HIV-1 in tissue culture and their selectivity indexes were 42, 40, 14, 18 and 65, respectively. To further fractionate Coptis chinensis, which is shown to be highest anti-HIV-1 activity, methanol extracts of Coptis chinensis were fractionated into methylene chloride at pH3, pH10 and water residue. The selectivity Indexes of CH$_2$C1$_2$(pH 3), CH$_2$C1$_2$(pH 10) and water residue were 50, 22 and 98 respectively. Our results show that the water residue of Coptis chinensis was the most effective for anti-HIV-1 activity.

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