• Title/Summary/Keyword: C. chinensis

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Inhibitory Activities of Palmatine from Coptis chinensis Against Helicobactor pylori and Gastric Damage

  • Jung, Joohee;Choi, Jae Sue;Jeong, Choon-Sik
    • Toxicological Research
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    • v.30 no.1
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    • pp.45-48
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    • 2014
  • Helicobacter pylori (H. pylori) is the most important factor of gastric disease in clinical practice. Moreover, smoking, stress and a poor diet may be additive factors for gastric damage. With these factors, increasing infection of H. pylori triggers gastritis, gastric ulcers and gastric cancer. To develop a new protective agent, we are concerned with plant-derived extract. The extract of Coptis chinensis (C. chinensis) and its constituents were investigated to assess their protective activities against gastric damage. The C. chinensis extract showed a scavenging effect against 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radicals, inhibition of H. pylori colonization and antiulcerogenic activities in rat. In particular, palmatine derived from C. chinensis was found to be the novel protective agent. It is better than the C. chinensis extract, berberine, a well-known constituent of C. chinensis. We suggest that palmatine from the root cortex of C. chinensis may be a good candidate for the development of new pharmaceuticals to prevent gastric disease.

Inhibition of Candida albicans Biofilm Formation by Coptidis chinensis through Damaging the Integrity of Cell Membrane (세포막손상 유발로 인한 황련의 캔디다 바이오필름 형성 억제)

  • Kim, Younhee
    • Korean Journal of Microbiology
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    • v.49 no.1
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    • pp.17-23
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    • 2013
  • Candida biofilms are organized microbial communities growing on the surfaces of host tissues or indwelling medical devices, and the biofilms show enhanced resistance against the conventional antifungal agents. The roots of Coptidis chinensis have been widely used for medicinal purposes in East Asia. The present study was aimed to assess the effect of C. chinensis aqueous extract upon preformed biofilms of 10 clinical Candida albicans isolates and the antifungal activities which contribute to inhibit the C. albicans biofilm formation. Its effect on preformed biofilms was judged using XTT [2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide)] reduction assay, and metabolic activity of all tested strains was reduced significantly ($57.3{\pm}14.7%$) at $98{\mu}g/ml$ of the C. chinensis extract. The extract damaged the cell membrane of C. albicans which was analyzed by fluorescein diacetate and propidium iodide staining. The anticandidal activity was fungicidal, and the extract obstructed the adhesion of C. albicans biofilms to polystyrene surfaces, arrested C. albicans cells at $G_o/G_1$ as well, and reduced the growth of biofilms or budding yeasts finally. The data suggest that C. chinensis has multiple antifungal effects on target fungi resulting in preventing the formation of biofilms. Therefore, C. chinensis holds great promise for exploring antifungal agents from natural products in treating and eliminating biofilm-associated Candida infection.

Antibacterial Activity of Caesalpinia sappan and Coptis chinensis Extracts against Bacillus cereus and Vibrio parahaemolyticus (소목(蘇木)과 황련(黃連) 추출물(抽出物)의 Bacillus cereus와 Vibrio parahaemolyticus 에 대한 항세균활성(抗細菌活性))

  • Lee, Gun-Hee;Doh, Eun-Soo;Chang, Jun-Pok;Kil, Gi-Jung
    • The Korea Journal of Herbology
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    • v.25 no.3
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    • pp.111-116
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    • 2010
  • Objectives : This experimental study was performed to investigate the antibacterial effect of Caesalpinia sappan and Coptis chinensis extract against B. cereus and V. parahaemolyticus. Methods : Methanol extracts of C. sappan and C. chinensis was tested against B. cereus and V. parahaemolyticus by paper disc method. Results : The growth of B. cereus and V. parahaemolyticus was inhibited by C. sappan and C. chinensis extract among 6 kinds of medicinal plant extracts. The extract of C. sappan and C. chinensis extract inhibited the growth of V. parahaemolyticus and B. cereus, respectively. The growth of B. cereus and V. parahaemolyticus had a tendency to increase depend on the concentration of the extract. EtOEt and EtOAc fractions and EtOEt and BuOH fractions of the C. sappan extract had a high antibacterial activity against B. cereus and V. parahaemolyticus, respectively. And, BuOH and $H_2O$ fractions of the C. chinensis extract showed antibacterial activity against B. cereus highly. Conclusions : C. sappan and C. chinensis extract efficiently inhibited the growth of B. cereus and V. parahaemolyticus.

Inhibitory effects of Coptis chinensis extract on the growth and biofilm formation of Streptococcus mutans and Streptococcus sobrinus

  • Kim, Si Yeong;Song, Yuri;Lee, Hyun Ah;Na, Hee Sam;Jung, Chul Jong;Bek, Gyung Yun;Chung, Jin
    • International Journal of Oral Biology
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    • v.45 no.4
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    • pp.143-151
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    • 2020
  • Streptococcus mutans and Streptococcus sobrinus play important roles in dental caries. Coptis chinensis is a natural product with antimicrobial activity against enterobacteria; however, its effects on oral streptococci are still unknown. Therefore, the effects of C. chinensis on the growth and biofilm formation of the representative cariogenic bacteria S. mutans and S. sobrinus were investigated for the possible use of C. chinensis as an anticaries agent. The C. chinensis extract was diluted with sterile distilled water, and 0.1-2.5% of the extract was used in the experiment. The effects of the C. chinensis extract on the growth and glucan formation of S. mutans and S. sobrinus were measured by viable cell counting and spectrophotometry at 650 nm absorbance, respectively. Crystal violet staining was also carried out to confirm the C. chinensis extract's inhibitory effect on biofilm formation. The C. chinensis extract significantly inhibited the growth of S. mutans and S. sobrinus at concentrations of ≥ 0.3% as compared with the control group. The viable cell count of colonies decreased by 1.7-fold and 1.2-fold at 2.5% and 1.25%, respectively, compared with the control group. The biofilm formation of S. mutans and S. sobrinus was inhibited by > 20-fold at C. chinensis extract concentrations of ≥ 1.25% as compared with the control group. In summary, the C. chinensis extract inhibited the growth and biofilm and glucan formation of S. mutans and S. sobrinus. Therefore, C. chinensis might be a potential candidate for controlling dental caries.

Antioxidant Activity of Cercis chinensis and Its Protective Effect on Skin Aging

  • Na, Min-Kyun;Bae, Ki-Hwan;Hong, Nam-Doo;Yoo, Jae-Kuk;Nobuhiko Miwa
    • Proceedings of the SCSK Conference
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    • 2003.09a
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    • pp.291-312
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    • 2003
  • Reactive oxygen species are capable of damaging biomolecules such as lipids, proteins, and DNA, which can not only lead to various diseases, but also oxidative damage resulting aging. In our previous study, Cercis chinensis (Leguminosae) showed a potent antioxidant activity. Nineteen compounds were isolated through antioxidant activity-guided fractionation. The C. chinensis extract and some of the constituents exhibited a potent antioxidant activity on the free radicals and lipid peroxidation and a notable protective effect on the t-BuOOH induced oxidative damage. In vivo test of skin damage induced by UVB irradiation, the extract of C. chinensis and a constituent, piceatannol, exhibited a significant protective effect. The life-span of the HEK-N/F cells were extended by 1.21-2.12 fold as a result of the continuous administration of 3 $\mu\textrm{g}$/ml of the C. chinensis extract and the active constituents compared to that of the control. These observations were attributed to the inhibitory effect of the C. chinensis extract and its constituents on the age-dependent shortening of the telomere. Thus, C. chinensis was demonstrated to protect the skin cells against oxidative stress and inhibit thereby the cellular aging, followed by expectation as anti-aging cosmetic ingredient.

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Antioxidant Activity of Cercis chinensis and Its Protective Effect on Skin Aging

  • Na, Min-Kyun;Bae, Ki-Hwan;Hong, Nam-Doo;Yoo, Jae-Kuk;Nobuhiko Miwa
    • Proceedings of the SCSK Conference
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    • 2003.09b
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    • pp.117-138
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    • 2003
  • Reactive oxygen species are capable of damaging biomolecules such as lipids, proteins, and DNA, which can not only lead to various diseases, but also oxidative damage resulting aging. In our previous study, Cercis chinensis (Leguminosae) showed a potent antioxidant activity. Nineteen compounds were isolated through antioxidant activity-guided fractionation. The C. chinensis extract and some of the constituents exhibited a potent antioxidant activity on the free radicals and lipid peroxidation and a notable protective effect on the t-BuOOH induced oxidative damage. In vivo test of skin damage induced by UVB irradiation, the extract of C. chinensis and a constituent, piceatannol, exhibited a significant protective effect. The life-span of the HEK-N/F cells were extended by 1.21-2.12 fold as a result of the continuous administration of 3 $\mu\textrm{g}$/ml of the C. chinensis extract and the active constituents compared to that of the control. These observations were attributed to the inhibitory effect of the C. chinensis extract and its constituents on the age-dependent shortening of the telomere. Thus, C. chinensis was demonstrated to protect the skin cells against oxidative stress and inhibit thereby the cellular aging, followed by expectation as antiaging cosmetic ingredient.

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Development and Validation of HPLC-PDA Method and Pattern Recognition Analyses Using Eight Marker Compounds for the Quality Control Between the Seeds of Cuscuta chinensis Lam. and Cuscuta japonica Choisy

  • Nguyen, Duc Hung;Zhao, Bing Tian;Le, Duc Dat;Ma, Eun Sook;Min, Byung Sun;Woo, Mi Hee
    • Natural Product Sciences
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    • v.25 no.4
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    • pp.334-340
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    • 2019
  • Cuscuta chinensis Lam. and Cuscuta japonica Choisy are parasitic plants. C. chinensis seeds were traditionally used for treatment of kidney and liver deficiencies. C. japonica seeds were used as tonic medicine to improve liver function and strengthen kidneys, treatment of high blood pressure, chronic diarrhea, and sore eyes. Cuscutae Semen are seeds of only C. chinensis in Korean Herbal Pharmacopoeia (K.H.P.). The developed HPLC-PDA method easily, accurately, and sensitively quantified using eight marker compounds [hyperoside (1), astragalin, (2), quercetin (3), kaempferol (4), chlorogenic acid (5), 3,4-di-O-caffeoylquinic acid (6), 1,5-di-O-caffeoylquinic acid (7), and 4,5-di-O-caffeoylquinic acid (8)]. In addition, the method may be used to distinguish seeds between C. chinensis Lam. and C. japonica Choisy. Furthermore, the result from the current study was applied to clarify samples between steam processed and unprocessed samples of C. chinensis by pattern analysis.

Inhibition of HIV-1 Replication by Extract of Coptis chinensis (황련추출물에 의한 HIV-1 복제 저해)

  • 송만기;이안휘;김영호;이정준;성영철
    • Biomolecules & Therapeutics
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    • v.3 no.2
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    • pp.111-115
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    • 1995
  • Natural products, total number of 175, were screened to test for their effect on the replication of human immunodeficiency virus type 1 (HIV-1). Five of them, such as Eriobotrya japonica, Eugenia caryphyllata, Cuscuta chinensis, Glycyrrhiza uralensis, and Coptis chinensis were shown to be effective in inhibiting the replication of HIV-1 in tissue culture and their selectivity indexes were 42, 40, 14, 18 and 65, respectively. To further fractionate Coptis chinensis, which is shown to be highest anti-HIV-1 activity, methanol extracts of Coptis chinensis were fractionated into methylene chloride at pH3, pH10 and water residue. The selectivity Indexes of CH$_2$C1$_2$(pH 3), CH$_2$C1$_2$(pH 10) and water residue were 50, 22 and 98 respectively. Our results show that the water residue of Coptis chinensis was the most effective for anti-HIV-1 activity.

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Effect of Schizandra chinensis and Pine Meddle on Growth of Pathogens Relate to Acne. (여드름 원인균의 성장에 미치는 오미자와 솔잎의 효과)

  • 성준모;박나영;이신호
    • Microbiology and Biotechnology Letters
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    • v.31 no.1
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    • pp.69-74
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    • 2003
  • Antimicrobial effect of Schizandra chinensis and pine neddle against various pathogens relate to acne (comedones). Ethanol extracts of Schizandra chinensis and pine neddle showed antimicrobial activity against Malassezia furfur Staphylococcus epidermidis and Propionibacterium acnes . The growth of M. fufur and S. epidermidis was inhibited completely by addition of 0.12% Schizandra chinensis extract to YM broth (YM) and tryptic soy broth (TSB) after 24 h incubation. The growth of Propionibacterium acnes was completely inhibited on YM and TSB containing 0.06% of ethanol extract of Schizandra chinensis and pine neddle, respectively. Minimum inhibitory concentration (MIC) of Schizandra chinensis and pine neddle against P. acnes was 0.0075% (75 $\mu\textrm{g}$/mL). The antimicrobial activities of Schizandra chinensis and pine noddle did not decrease apparantly by heat treatment at $80^{\circ}C$ for 30 min, 10$0^{\circ}C$ for 30 min and $121^{\circ}C$ for 15 min, respectively.

Isolation and Identification of Major Component from Roots of Potentilla chinensis (딱지꽃(Potentilla chinensis) 뿌리 추출물의 주요성분 분리동정)

  • Jung, Hae Soo;Kim, Hyoung Shik;Lee, Jeong Hun;Moh, Seo Jin;Yeo, Jin Hui;Park, Gi won;Moh, Sang Hyun
    • Journal of Applied Biological Chemistry
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    • v.59 no.1
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    • pp.5-7
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    • 2016
  • One of oriental medicinal plants, Potentilla chinensis, has been used for anti-inflammation, hemostatic, decryption, and antipyretic. Especially, a root of Potentilla chinensis was used as important material for oriental medication. Although several kinds of bioactive component of Potentilla chinensis extract from stems and leaves were identified, the major component of Potentilla chinensis from roots is not well established. In this study, the root of Potentilla chinensis was extracted in different solvent system and analyzed by high performance liquid chromatography (HPLC). According to HPLC analysis, a major component was isolated and its physicochemical properties were evaluated by mass spectrometry and nuclear magnetic resonance. Based on these results, isolated compound was identified as 2,3,8-Tri-O-methylellagic acid. And quantification of 2,3,8-Tri-O-methylellagic acid with different extraction solvent system was performed for industrial application.