• Title/Summary/Keyword: C&D

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Overexpressed Drosophila DNA Methyltransferase 2 Isoform C Interacts with Hsp70 in Vivo

  • Roder, Karim
    • BMB Reports
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    • v.40 no.4
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    • pp.554-561
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    • 2007
  • Shen and colleagues (Lin et al., 2004) have recently shown that overexpression of the Drosophila DNA methyltransferase 2 isoform C, dDnmt2c, extended life span of fruit flies, probably due to increased expression of small heat shock proteins such as Hsp22 or Hsp26. Here, I demonstrate with immunoprecipitations that overexpressed dDnmt2c interacts with endogenous Hsp70 protein in vivo in S2 cells. However, its C-terminal half, dDnmt2c(178-345) forms approximately 10-fold more Hsp70-containing protein complexe than wild-type dDnmt2c. Overexpressed dDnmt2c(178-345) but not the full length dDnmt2c is able to increase endogenous mRNA levels of the small heat shock proteins, Hsp26 and Hsp22. I provide evidence that dDnmt2c(178-345) increases Hsp26 promoter activity via two heat shock elements, HSE6 and HSE7. Simultaneously overexpressed Hsp40 or a dominant negative form of heat shock factor abrogates the dDnmt2c(178-345)-dependent increase in Hsp26 transcription. The data support a model in which the activation of heat shock factor normally found as an inactive monomer bound to chaperones is linked to the overexpressed C-terminus of dDnmt2c. Despite the differences observed in flies and S2 cells, these findings provide a possible explanation for the extended lifespan in dDnmt2c-overexpressing flies with increased levels of small heat shock proteins.

Taxonomy and distribution of Corycaeidae (Copepoda: Cyclopoida) in the Korean Waters in Summer (한국 연근해에서 하계에 출현하는 Corycaeidae (Copepoda : Cyclopoida)의 분류 및 분포)

  • KANG, YOUNG SHIL;HUH, SUNG-HOI;LEE, SAM SEUK
    • 한국해양학회지
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    • v.25 no.2
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    • pp.49-61
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    • 1990
  • The family Corycaeidae in the neighbouring seas of Korea was taxonomically studied based on the zooplankton samples collected in August, 1986. In this study, nine species belonging to two genera and three subgenera of the Corycaeidae were identified and described with illustrations; Corycaeus (Corycaeus) speciosus, G.(C.) crassiusculus, C. (Ditrichocorycaeus) affinis, C.(D.) andrewsi, C.(D.) erythraeus, C.(Onychocorycaeus) pacificus, c.(O.) catus, C.(O.) agilis, and Farranula gibbula. The distribution patterns of these species showed that C.(D.) affinis appeared to be and indicator species of the coastal waters, and C.(C.) speciosus, C.(C.) crassiusculus, c.(O.) catus, C.(D.) andrewsi, and Farranula gibbula appeared to be indicator species of the warm-oceanic waters.

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Studies on the Chemical Structure of the New Polysaccharide C - (The New Polysaccharides of Gum Tragacanth. II) - (Tragacanth gum 의 신다당류(新多糖類) C 의 화학구조(化學構造) - Tragacanth gum의 신다당류(新多糖類)에 관(關)한 연구(硏究) 제2보(第二報) -)

  • Lee, Sung-Hwan
    • Applied Biological Chemistry
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    • v.3
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    • pp.25-48
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    • 1962
  • The polysaccharide C prepared from gum tragacanth powder (U. S. P. grade) by the precipitation method with 85% ethanol was a neutral polysaccharide, $[{\alpha}]^{30}_D-72.2$. The polysaccharide C consisted of L-rhamnose, D-xylose, L-arabinose and D-galactose in the molar ratio 2:1:17:9 (Table 1, 2, 3, ). The polysaccharide C was methylated with dimethylsulphate and 40% NaOH, and Purdies regent. The hydrolyzate of fully methlated product ($[{\alpha}]^{22}_D-102$ in chloroform, the methoxy content 40.6%) was composed of 2, 3, 5-tri-O-methyl-L-arabofuranose (I), 3,4-di-O-methyl-L-rhamnopyranose (II), 2,3-di-O-methyl-D-xylose (III), 2,3,4-tri-O-methyl-D-galactopyranose (IV), 2,4-di-O-methyl-L-arabopyranose (?), 2,4-di-O-methyl-D-galactose(VI), 2-O-methyl-D-arabinose (VII), and L-arabopyranose(VIII) (Table 4, 5, and Fig. 4). The first partial hydrolysis (A) of the polysaccharide C with 0.05N-HCl for 4.5 hours at $80-85^{\circ}C$ released only L-arabinose: the second hydrolysis (B) with 0.1N-HCl for 5 hours at $80-85^{\circ}C$, L-arabinose and D-galactose; and the third hydrolysis (C) with 0.3N-HCl at $90-95^{\circ}C$ in sealed tube, L-rhamnose, D-xylose, L-arabinose and D-galactose. From the unhydrolyzate A' were found L-rhamnose, D-xylose, L-arabinose, and D-galactose; from B' L-rhamnose, d-xylose, L-arabinose and D-galactose; and from C' D-xylose and D-galactose respectively (Table 6). The periodate consumption and formic acid production of the polysaccharide C were measured at various time intervals. After 120 hours periodat was consumed by 1.23 mole per $C_5H_8O_4$ and formic acid was produced 0.78 mole per $C_5H_8O_4$ (Table 7). Although a definite chemical structure for this polysaccharide C may not be formulated, experimental data, especially, from methylation, partial hydrolysie and determination of its molar ratio, and periodate analysis showed that the polysaccharide C is a highly branched polysaccharide and would be constructed of galactoaraban as a main chain residue and L-arabofuranose, D-galactopyranosyl $(1{\rightarrow}1)$-L-arabofuranose, D-xylopyranosyl $(1{\rightarrow}2)$-L-rhamnopyranosyl $(1{\rightarrow}1)$-L-arabofuranose, and L-rhamnopyranosyl $(1{\rightarrow}1)$-arabofuranose, and D-galactopyranosyl-$(1{\rightarrow}2)$-L-arabopyranosyl-$(1{\rightarrow}1)$-I-arabofuranose as a branch chain or end group (page 21).

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The Effect of Salicylic Acid on $Cd^{2+}$-induced Physiological Toxicity in Commelina communis L. ($Cd^{2+}$에 의한 닭의장풀의 생리적 독성에 salicyclic acid가 미치는 영향)

  • 이준상
    • Korean Journal of Environmental Biology
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    • v.20 no.1
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    • pp.73-77
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    • 2002
  • The effect of salicylic acid (SA) on C $d^{2+}$ - induced physiological toxicity in Commelina communis was investigated. 3- weeks old Commelina communis was transferred to and grown in Hoagland solution in the presence or absence of 100 $\mu$M C $d^{2+}$ and SA for 3 weeks. In the treatment of C $d^{2+}$ + SA, the length of stem was increased to 0.7 cm for 3 weeks (C $d^{2+}$, 2.1cm; control, 7.2 cm). C $d^{2+}$ + SA reduced total chlorophyll content up to 86%, and changed chlorophyll a/b ratio below 1.6. C $d^{2+}$ + SA also reduced about 40-78% of water potential, but C $d^{2+}$ increased 16-39% from 1 week to 3 weeks. C $d^{2+}$ + SA also inhibited 27% of Fv/Fm, but in case of C $d^{2+}$, Fv/Fm was not changed. The treatment of C $d^{2+}$ + SA showed about 37-58% inhibition of photosynthetic activity when measured at various light intensity (500-1000 $\mu$mol $m^{-2}$ $s^{-1}$ ). In the case of C $d^{2+}$ treatment, photosynthetic activity was inhibited to 12-15%. Similar effect was found in terms of stomatal conductance. Therefore, it could be concluded that the treatment of C $d^{2+}$ + SA into plant decrease or block various physiological activities and lend to die by double effects of both chemicals.cts of both chemicals..

위상변성기에 의한 새로운 주파변환기의 특성

  • 오상세
    • 전기의세계
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    • v.13 no.1
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    • pp.20-23
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    • 1964
  • 본 변환기는 교번자계의 원리에 의한 보통 변환기가 아니고 회전자계의 원리를 이용한 변환기로서 상변환, 주파수변화, A.C.에서 D.C. 전원변환, D.C.에서 A.C. 전원변환, 속도제어, 진상기 등을 얻을 수 있는 경제적이고 능률적인 변환기를 얻을 수 있다. 임의의 다상을 얻을 수 있는 이 변환기는 다상 전원을 필요로 하는 수은정류기 등에 응용할 수 있고 A.C.전원에서 D.C.전원의 대용량전원을 얻어 D.C.전원이 필요로 하는 직류전철의 전원, 직류송전의 전원등에 이용할 수 있고, D.C.전원에서 A.C.전원을 얻어 교류전력기를 운전할 수 있으며 D.C.송전선에서 A.C.로 변환하여 종전의 A.C.배전선에 공급하면 D.C.송전을 이용한 송전손실을 경감시킬수 있고 주파수를 변환하여 속도제어를 하는 등 광범위하게 그 응용이 가능한 것이다.

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Meaning of the Expression a:b=c:d and Implications for Teaching (비례식 a:b=c:d의 의미 분석과 학습 지도에의 시사점)

  • Yim, Jaehoon
    • Journal of Elementary Mathematics Education in Korea
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    • v.23 no.3
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    • pp.273-288
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    • 2019
  • This study focuses on understanding proportionality, in particular, what constitutes relational understanding of a:b=c:d, which is called proportional expression. The meanings of a:b=c:d are analyzed and some suggestions are offered for improving the teaching and learning of it. The equation a:b=c:d has three different meanings. First, it represents two different structures in one proportional situation. Second, it represents a common structure in two different proportional situations. Finally, it represents a number or a quantity underlying in different proportional situations. It is important to choose and use a unit flexibly to understand the first and the second meanings of a:b=c:d, Double strip diagram and double number line are useful to visualize the meanings of a:b=c:d. In addition, what a number or a quantity in the third meaning of a:b=c:d refers to in proportional situations should be emphasized in teaching and learning of a:b=c:d.

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Embedding Algorithm between Folded Hypercube and HFH Network (폴디드 하이퍼큐브와 HFH 네트워크 사이의 임베딩 알고리즘)

  • Kim, Jongseok;Lee, Hyeongok;Kim, Sung Won
    • KIPS Transactions on Computer and Communication Systems
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    • v.2 no.4
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    • pp.151-154
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    • 2013
  • In this paper, we will analyze embedding between Folded Hypercube and HFH. We will show Folded Hypercube $FQ_{2n}$ can be embedded into HFH($C_n,C_n$) with dilation 4, expansion $\frac{(C_n)^2}{2^{2n}}$ and HFH($C_d,C_d$) can be embedded into $FQ_{4d-2}$ with dilation O(d).

A study on comparative analysis of direct current control in A.C.-D.C. interconnected power system (교류-직류 연계계통에 있어서 직류제어방식의 비교연구)

  • 정형환;왕용필;안병철;이광우
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.45 no.4
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    • pp.474-483
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    • 1996
  • In this paper, as a part of the method improving stability, the load-flow calculation in D.C. power system and the models for stability analysis are studied with A.C-D.C. interconnected power systems transmission performed. Moreover, the theory is established in relation to each control method of D.C. power systems. Then the stability of A.C-D.C.interconnected power systems is compared and considered by the way of dividing the operating control method of the rectifier inverter converter into ACR-AVR, APR-A.gamma.R, A.alpha.R-ACR. The dynamics characteristic of terminal voltage, frequency, active-reactive power and rotor angle of the generator with disturbances and load fluctuations is considered. In addition, the characteristic of direct voltage, direct current, power and control systems. From this the comparative analysis of the direct current control method, the possibility of the stability analysis of A.C.-D.C. interconnected power system is considered. (author). refs., figs., tabs.

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THE INDEX OF THE CORESTRICTION OF A VALUED DIVISION ALGEBRA

  • Hwang, Yoon-Sung
    • Journal of the Korean Mathematical Society
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    • v.34 no.2
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    • pp.279-284
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    • 1997
  • Let L/F be a finite separable extension of Henselian valued fields with same residue fields $\overline{L} = \overline{F}$. Let D be an inertially split division algebra over L, and let $^cD$ be the underlying division algebra of the corestriction $cor_{L/F} (D)$ of D. We show that the index $ind(^cD) of ^cD$ divides $[Z(\overline{D}) : Z(\overline {^cD})] \cdot ind(D), where Z(\overline{D})$ is the center of the residue division ring $\overline{D}$.

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YlaC is an Extracytoplasmic Function (ECF) Sigma Factor Contributing to Hydrogen Peroxide Resistance in Bacillus subtilis

  • Ryu Han-Bong;Shin In-Ji;Yim Hyung-Soon;Kang Sa-Ouk
    • Journal of Microbiology
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    • v.44 no.2
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    • pp.206-216
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    • 2006
  • In this study, we have attempted to characterize the functions of YlaC and YlaD encoded by ylaC and ylaD genes in Bacillus subtilis. The GUS reporter gene, driven by the yla operon promoter, was expressed primarily during the late exponential and early stationary phase, and its expression increased as the result of hydrogen peroxide treatment. Northern and Western blot analyses revealed that the level of ylaC transcripts and YlaC increased as the result of challenge with hydrogen peroxide. A YlaC-overexpressing strain evidenced hydrogen peroxide resistance and a three-fold higher peroxidase activity as compared with a deletion mutant. YlaC-overexpressing and YlaD-disrupted strains evidenced higher sporulation rates than were observed in the YlaC-disrupted and YlaD-overexpressing strains. Analyses of the results of native polyacrylamide gel electrophoresis of recombinant YlaC and YlaD indicated that interaction between YlaC and YlaD was regulated by the redox state of YlaD in vitro. Collectively, the results of this study appear to suggest that YlaC regulated by the YlaD redox state, contribute to oxidative stress resistance in B. subtilis.