• Asian-Australasian Journal of Animal Sciences
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• 제31권10호
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• pp.1677-1684
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• 2018

Objective: To find biological functions such as antibacterial and antioxidant activities in several tropical plants and to investigate the possibility of antibiotic substitute agents to prevent and treat diseases caused by pathogenic bacteria. Methods: Plants such as Poncirus trifoliata fruit (Makrut), Zingiber officinale Rosc (Khing), Areca catechu L. (Mak), Solanum melongena L. I (Makkhuayao), and Solanum melongena L. II (Makhurapro) were extracted by methanol, n-hexane, chloroform, ethyl acetate, butanol and water. The free radical scavenging activities were measured using 2-diphenyl-2-picryl hydrazyl photometric assay. Antibacterial activities with a minimum inhibitory concentration (MIC) were observed by agar diffusion assay against pathogenic strains of Escherichia coli, Burkholderia sp., Haemopilus somnus, Haemopilus parasuis, Clostridium perfringens, and Pantoea agglomerans. Results: Poncirus trifoliata fruit methanol extract showed antibacterial activities against gram-negative and gram-positive pathogens. Additionally, this showed the strongest antibacterial activity against Burkholderia sp. and Haemopilus somnus with MIC $131{\mu}g/mL$, respectively. Areca catechu L. water extract showed antibacterial activities against Burkholderia sp., Haemopilus somnus, and Haemopilus parasuis. The MIC value for Haemopilus parasuis was $105{\mu}g/mL$ in this. Antioxidant activity of Zingiber officinale Rosc n-hexane extract showed 2.23 mg/mL effective concentration 50% ($EC_{50}$) value was the highest activity among tropical plants extracts. Total polyphenol content in Zingiber officinale Rosc methanol extract was $48.4{\mu}g/mL$ and flavonoid content was $22.1{\mu}g/mL$ showed the highest values among tested plants extracts. Conclusion: Taken together, these results suggest that tropical plants used in this study may have a potential benefit as an alternative antibiotics agent through their antibacterial and antioxidant activities.

• Journal of Microbiology
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• 제45권5호
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• pp.409-417
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• 2007

Burkholderia sp. HY-10 isolated from the digestive tracts of the longicorn beetle, Prionus insularis, produced an extracellular lipase with a molecular weight of 33.5 kDa estimated by SDS-PAGE. The lipase was purified from the culture supernatant to near electrophoretic homogenity by a one-step adsorption-desorption procedure using a polypropylene matrix followed by a concentration step. The purified lipase exhibited highest activities at pH 8.5 and $60^{\circ}C$. A broad range of lipase substrates, from $C_4\;to\;C_{18}$ p-nitrophenyl esters, were hydrolyzed efficiently by the lipase. The most efficient substrate was p-nitrophenyl caproate ($C_6$). A 2485 bp DNA fragment was isolated by PCR amplification and chromosomal walking which encoded two polypeptides of 364 and 346 amino acids, identified as a lipase and a lipase foldase, respectively. The N-terminal amino acid sequence of the purified lipase and nucleotide sequence analysis predicted that the precursor lipase was proteolytically modified through the secretion step and produced a catalytically active 33.5 kDa protein. The deduced amino acid sequence for the lipase shared extensive similarity with those of the lipase family 1.2 of lipases from other bacteria. The deduced amino acid sequence contained two Cystein residues forming a disulfide bond in the molecule and three, well-conserved amino acid residues, $Ser^{131},\;His^{330},\;and\;Asp^{308}$, which composed the catalytic triad of the enzyme.

• 한국토양비료학회지
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• 제39권4호
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• pp.195-203
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• 2006

길항미생물 MP-1은 여러 종의 곰팡이와 효모 및 음식물을 부패시키는 세균 (그람양성 및 음성) 에 대하여 항균활성을 보였다. MP-1의 16S rRNA gene 염기서열은 기존에 밝혀진 Brukholderia sp.의 염기서열과 99-100% 유사하였다. 배양액으로부터의 항균물질은 에틸아세테이트 (EtOAc)를 사용하여 분리하였으며 EtOAc에 용해된 산성 분획은 silica gel, Sephadex LH-20, ODS 컬럼크로마토 그래피 및 HPLC를 사용하여서 정제를 실시 하였다. 최종적으로 Gas chromatographic-mass 스펙트럼을 통한 분석을 통하여 4 종의 항균 활성을 나타내는 물질은 phenylacetic acid, hydrocinnamic acid, 4-hydroxyphenylacetic acid 및 4-hydroxyphenylacetate methyl ester로 확인 되었다. 각각의 항균 활성물질들의 미생물에 대한 최소저해농도(MIC)는 $250{\sim}2500ug\;ml^{-1}$ 인 것으로 나타났다. 또한 6 mm paper disc 에서의 1mg (건물중) 산성 분획의 항균활성은 같은 비율로 혼합한 표준혼합용액 보다 더 효과적인 것으로 나타났다.

• 농약과학회지
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• 제18권1호
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• pp.21-25
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• 2014

Burkholderia pyrrocinia CAB08106-4은 Sclereotium cepivorum과 Sclereotium sp. 균주에 의해 발생하는 마늘 흑색 썩음균핵병에 대한 항박테리아 효능을 가지고 있다. 이는 Sclereotium cepivorum과 Sclereotium sp.에 의해 발생하는 마늘 흑색썩음 균핵병을 포함한 다양한 식물병원체를 관리하고 예방하기 위하여 개발중인 친환경적인 미생물 제품이다. 본 연구에서는 마늘 흑색 썩음균핵병의 방제재로 개발중인 Bukholderia pyrrocinia CAB08106-4 원제($1.0{\times}10^9cfu/mL$)가 잉어와 물벼룩에 미치는 영향을 평가하였으며, 그 결과 $6.67{\times}10^4cfu/mL$의 농도에서는 독성이 관찰되지 않았다. 따라서 Burkholderia pyrrocinia CAB08106-4 원제는 담수어류 및 담수무척추동물에 영향이 낮을 것으로 기대된다.

• The Plant Pathology Journal
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• 제33권2호
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• pp.148-162
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• 2017

As a step towards discovering novel pathogenesis-related proteins, we performed a genome scale computational identification and characterization of secreted and transmembrane (TM) proteins, which are mainly responsible for bacteria-host interactions and interactions with other bacteria, in the genomes of six representative Burkholderia species. The species comprised plant pathogens (B. glumae BGR1, B. gladioli BSR3), human pathogens (B. pseudomallei K96243, B. cepacia LO6), and plant-growth promoting endophytes (Burkholderia sp. KJ006, B. phytofirmans PsJN). The proportions of putative classically secreted proteins (CSPs) and TM proteins among the species were relatively high, up to approximately 20%. Lower proportions of putative type 3 non-classically secreted proteins (T3NCSPs) (~10%) and unclassified non-classically secreted proteins (NCSPs) (~5%) were observed. The numbers of TM proteins among the three clusters (plant pathogens, human pathogens, and endophytes) were different, while the distribution of these proteins according to the number of TM domains was conserved in which TM proteins possessing 1, 2, 4, or 12 TM domains were the dominant groups in all species. In addition, we observed conservation in the protein size distribution of the secreted protein groups among the species. There were species-specific differences in the functional characteristics of these proteins in the various groups of CSPs, T3NCSPs, and unclassified NCSPs. Furthermore, we assigned the complete sets of the conserved and unique NCSP candidates of the collected Burkholderia species using sequence similarity searching. This study could provide new insights into the relationship among plant-pathogenic, humanpathogenic, and endophytic bacteria.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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• pp.201.1-201
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• 2005

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2001년도 제31회 학술심포지움 및 춘계학술대회
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• pp.61-61
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• 2001

• 한국약용작물학회지
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• 제14권4호
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• pp.202-205
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• 2006

인삼의 안정적인 생산 및 합리적인 병해방제에 기여하기 위하여 연구를 실시한 결과 갈잎 퇴비에서 분리된 Streptomyces lauretii B8180과 Bacillus subtilis B8856, Burkholderia cepacia B7944 세 균주의 인삼병원균 5종에 대한 항균력이 인정되었으며, 특히 실내실험을 통하여 높은 역병 방제효과가 인정되었다. 그러나 이 실험에서와 같이 식물의 지상부에 길항균을 살포하는 방법을 통하여 역병 방제효과를 높일 수 있을 것으로 생각된다. 이 길항균들은 갈잎 퇴비에서 분리되었기 때문에, 갈잎 퇴비에서 증식시켜 인삼재배 예정지에 투입하면, 억제형 토양 (suppressive soil) 조성을 통한 주요병해 방제가능성도 클 것으로 생각된다.

• Journal of Microbiology and Biotechnology
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• 제12권1호
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• pp.77-83
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• 2002

A bacterial consortium capable of utilizing a variety of polycyclic aromatic hydrocarbons has been isolated from a former manufactured gas plant site. The consortium consisted of four members including Arthrobacter sp., Burkholderia sp., Ochrobacterium sp., and Alcaligenes sp., which were identified and characterized by the patterns of fatty acid methyl esters (FAME analysis) and carbon source utilization (BIOLOG system). With the individual members, the biodegradation characteristics of aromatic hydrocarbons depending on different growth substrates were determined. FAME analyses demonstrated that microbial fatty acid profiles changed to significant extents in response to different carbon sources, and hence, such shift profiles may be informative to characterize the biodegradation potential of a bacterium or microbial community.

• Journal of Microbiology
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• 제43권6호
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• pp.572-576
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• 2005

This study focused on detecting catabolic genes for polycyclic aromatic hydrocarbons (PAHs) distributed in the reed rhizosphere of Sunchon Bay, Korea. These marsh and mud environments were severely affected by human activities, including agriculture and fisheries. Our previous study on microbial roles in natural decontamination displayed the possibility that PAH-degrading bacteria, such as Achromobacter sp., Alcaligenes sp., Burkholderia sp. and Pseudomonas sp. play an important decontamination role in a reed rhizosphere. In order to gain further fundamental knowledge on the natural decontamination process, catabolic genes for PAH metabolism were investigated through PCR amplification of dioxygenase genes using soil genomic DNA and sequencing. Comparative analysis of predicted amino acid sequences from 50 randomly selected dioxygenase clones capable of hydroxylating inactivated aromatic nuclei indicated that these were divided into three groups, two of which might be originated from PAH-degrading bacteria. Amino acid sequences of each dioxygenase clone were a part of the genes encoding enzymes for initial catabolism of naphthalene, phenanthrene, or pyrene that might be originated from bacteria in the reed rhizosphere of Sunchon Bay.