• 한국산학기술학회논문지
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• 제11권10호
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• pp.3833-3838
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• 2010

뇌파는 시시각각 변하는 뇌 상태를 가장 효과적으로 측정하는 수단이다. 뇌는 신진대사의 근간인 호르몬과 직접적인 관계가 있으므로 뇌파와 호르몬간의 영향관계를 규명할 필요가 있다. 본 연구는 뇌파만으로 신진대사와 관련된 정보의 산출이 가능함을 밝히는 연구를 수행하였다. 주요 연구변수는, 2007년 3월 3일부터 5월 28일까지 측정한, 직장인 여성들의 스트레스 정보인 뇌파 항스트레스 지수와 코티졸 호르몬 농도이다. 이들 간의 영향관계를 밝히고자, 뇌파훈련 전후에 대한 t검증, 상관분석, 회귀분석을 주된 연구방법으로 수행하였으며, 다음과 같은 결과가 도출되었다. 첫째, 뇌파훈련에 의해 변수들의 변화에 대한 유의한 차이가 검증되었다. 둘째, 변수들 간에 상관성이 존재함을 규명하였다. 마지막으로 회귀분석에서, 변수들 간에 영향력이 있는 것으로 파악되었다. 그러므로 뇌파분석만으로도 호르몬 분석수준의 스트레스 정보를 제공할 수 있다는 결론에 도달하였다.

• Applied Biological Chemistry
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• 제38권1호
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• pp.37-41
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• 1995

쥐 뇌에서 연령증가에 따른 progesterone의 대사에 대하여 연구하였다. 그 기초실험으로 생후 5일된 암컷 쥐의 뇌를 균질기로 분쇄한 경우와 잘게다진 경우의 실험에서 잘게다진 경우가 더 좋은 활성을 보였으며 쥐뇌 0.3 g, 30분의 표준 실험조건을 결정하였다. 그 결과로 $5{\alpha}$-환원요소의 경우 생후 $5{\sim}14$일에 가장 활성이 높았으며 점차적으로 감소하여 6주 이후에는 거의 일정한 활성을 나타내었다. $3{\alpha}-hydroxy$스테로이드 산화 환원요소$(3{\alpha}-HSOR)$도 $5{\alpha}$-환원요소와 비슷한 형태의 활성을 보였다. 그러나 이때 $3{\beta}-HSOR$의 활성은 연령에 따라 변화가 없이 거의 일정함을 알 수 있었다. 따라서 연령에 따른 $5{\alpha}$-환원대사산물 생성의 감소는 $5{\alpha}$-환원효소의 감소와 $3{\alpha}-HSOR$의 감소에 의하여 일어남을 알 수 있었다.

• 한국동물학회지
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• 제7권2호
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• pp.25-36
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• 1964

The present paper deals with the comparative study on phylogenic difference in the patterns of energy metabolism of brain slices of several vertebrate species by measuring oxygen consumptionwith glucose-6-phosphate, glucose-1-phosphate, glyceraldehyde-3-phosphate or glutamate as respiratory substrate employing Warburg's manometric method, by determination of the utilization rate of glucose using glucose-1-C14 by analyzing patterns of free amino acid distribution , and by histochemical determination using glucose-1-C14 by analyzing patterns of free amino acid distribution acid distribution , and by histochemical determination of glycogen contents. 1. Glucose enhances the oxygen consumption of brain slices of animals belinging to reptile, aves and mammalia while it shows a tendency to decrease that of animals belonging to pisces and amphibia. 2. Glucose-6--phosphate increase oxygen consumption more than glucose in every species examined, while glucose-1-phosphate and glyceraldehyde-3-phosphate increase that of Rana nigromaculata only . In general m, it appears that phosphosugars are more effective as a respiratory substrate to those species which have less endogenous respiration than to those having larger endogenous respiration. 3. Similar patterns of free amino acid distribution and the relative amount are found among the species and in every species examined glutamic acid is detected in the larges amount . ${\gamma}$-Amino butyric acid, glycine, alanine and aspartic acid are found in every species. 4. Ophicephalus showed less oxygen consumption than endogenous respiration when glutamate was added to the medium. When sodium fluoride was added, the oxygen consumption was some what increased . Such phenomenon wasnot found in the frog. 5. The result of histochemcial analysis of the brain showed that glycogen was abundantly present in the fish , amphibia , and especially in the reptile and that no distinctive grains of glycogen were found in the bird and mammal . From these facts, it may be supposed that anaerobic glycolysis as energy source dominates in fish and amphibia and aerobic respiration through the oxidation of glucose dominates in bird and mamal , the reptile occupying transitional position between these two categories. The way of obtaining energy for brain activity by the oxidation of glucose supplied from the circulating blood is seemed to be first acquired by reptile and the function is completed both in aves and mammal.

• Journal of Nutrition and Health
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• 제40권1호
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• pp.14-23
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• 2007

Elevated plasma homocysteine (Hcy) is a risk factor for cognitive dysfunction and Alzheimer disease, although the mechanism is still unknown. Both folate and betaine, a choline metabolite, play essential roles in the remethylation of Hcy to methionine. Choline deficiency may be associated with low folate status and high plasma Hcy. Alterations in DNA methylation also have established critical roles for methylation in development of the nervous system. This study was undertaken to assess the effect of choline and folate deficiency on Hcy metabolism and genomic DNA methylation status of the liver and brain. Groups of adult male Sprague Dawley rats were fed on a control, choline-deficient (CD), folate-deficient (FD) or choline/folate-deficient (CFD) diets for 8 weeks. FD resulted in a significantly lower hepatic folate (23%) (p<0.001) and brain folate (69%) (p<0.05) compared to the control group. However, plasma and brain folate remained unaltered by CD and hepatic folate reduced to 85% of the control by CD (p<0.05). Plasma Hcy was significantly increased by FD $(18.34{\pm}1.62{\mu}M)$ and CFD $(19.35{\pm}3.62{\mu}M)$ compared to the control $(6.29{\pm}0.60{\mu}M)$ (p<0.001), but remained unaltered by CD. FD depressed S-adenosylmethionine (SAM) by 59% (p<0.001) and elevated S-adenosylhomocysteine (SAM) by 47% in liver compared to the control group (p<0.001). In contrast, brain SAM levels remained unaltered in CD, FD and CFD rats. Genomic DNA methylation status was reduced by FD in liver (p<0.05) Genomic DNA hypomethylation was also observed in brain by CD, FD and CFD although it was not significantly different from the control group. Genomic DNA methylation status was correlated with folate stores in liver (r=-0.397, p<0.05) and brain (r = -0.390, p<0.05), respectively. In conclusion, our data demonsoated that genomic DNA methylation and SAM level were reduced by folate deficiency in liver, but not in brain, and correlated with folate concentration in the tissue. The fact that folate deficiency had differential effects on SAM, SAH and genomic DNA methylation in liver and brain suggests that the Hcy metabolism and DNA methylation are regulated in tissue-specific ways.

• Natural Product Sciences
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• 제16권3호
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• pp.148-152
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• 2010

Microbial metabolism studies of yangonin (1), a major styryl lactone from Piper methysticum, have resulted in the production of three hydroxylated metabolites (2-4). The chemical structures of these compounds were elucidated to be 4-methoxy-6-(12-hydroxystyryl)-2-pyrone (2),4-methoxy-6-(11,12-dihydroxystyryl)-2-pyrone (3),and 4,12-dimethoxy-6-(7,8-dihydroxy-7,8-dihydrostyryl)-2-pyrone (4) on the basis of the chemical and spectroscopic analyses. The compounds 3 and 4 are reported herein as microbial metabolites of yangonin for the first time.

• Mass Spectrometry Letters
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• 제10권1호
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• pp.11-17
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• 2019

Drosophila melanogaster (fruits fly) is a representative model system widely used in biological studies because its brain function and basic cellular processes are similar to human beings. The whole head of the fly is often used to obtain the key function in brain-related diseases like degenerative brain diseases; however the biomolecular distribution of the head may be slightly different from that of a brain. Herein, lipid profiles of the head and dissected brain samples of Drosophila were studied using electrospray ionization-mass spectrometry (ESI-MS). According to the sample types, the detection of phospholipid ions was suppressed by triacylglycerol (TAG), or the specific phospholipid signals that are absent in the mass spectrum were measured. The lipid distribution was found to be different in the wild-type and the microRNA-14 deficiency model ($miR-14{\Delta}^1$) with abnormal lipid metabolism. A few phospholipids were also profiled by comparison of the head and the brain in two fly model systems. The mass spectra showed that the phospholipid distributions in the $miR-14{\Delta}^1$ model and the wild-type were different, and principal component analysis revealed a correlation between some phospholipids (phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidylserine (PS)) in $miR-14{\Delta}^1$. The overall results suggested that brain-related lipids should be profiled using fly samples after dissection for more accurate analysis.

• 대한자기공명의과학회:학술대회논문집
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• 대한자기공명의과학회 1997년도 제2차 추계학술대회 초록집
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• pp.24-24
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• 1997

• 한국환경성돌연변이발암원학회:학술대회논문집
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• 한국환경성돌연변이발암원학회 2004년도 춘계학술대회
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• pp.65-65
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• 2004

• 대한핵의학회지
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• 제38권3호
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• pp.241-252
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• 2004

목적: 다수의 뇌 자료를 기반으로 구성된 통계적 화률뇌지도는 복잡하고 개인적인 편차가 큰 뇌의 형태학적, 기능적 특성을 분석하는데 유용하다. 특히 최근에 한국인의 정상 MR 영상을 기반으로 한 구조적 기능적 뇌 표준판과 구조적 확률뇌지도가 완성되었으며. 부검뇌의 조직절편을 활용한 세포구축학적 확률뇌지도가 도입되었다. 이 연구에서는 이들 자료를 활용하여 뇌 영상의 국소계수를 객관적으로 측정하기 위한 정량화 기법을 개발하였으며, 이를 이용하여 정상 한국인의 뇌 영상자료를 분석하였다. 대상 및 방법 : T1 MRI와 FDG PET에 대한 뇌 표준판을 성별, 연령별로 개발하였으며, 한국인 정상 MR 영상으로 만들어진 89개 뇌 영역의 구조적 확률뇌지도와 독일 율리히 연구센터에서 도입한 13개 브로드만 영역에 대한 세포구축학적 확률뇌지도를 뇌 표준판 위로 각각 비선형 변환하였다. 확률뇌지도에 정의된 각 뇌 영상의 국소계수는 확률에 의한 가중평균 또는 가중합으로 구하였다. 확률뇌지도를 이용한 예비 연구로 정상 노화에 따른 포도당대사의 변화가 대상회 내에서 전 후 위치에 따라 다르게 나타나는 가를 조사하였다. 결과: SPM과의 연계된 사용을 고려하여 Matlab 상에서 작성된 정량화 프로그램은 20초 미만에 1개의 공간정규화된 영상을 처리할 수 있었다. 대상회 포도당 대사에 대한 분석에서 후대상회에 대한 문측-전대상회 및 미측-전대상회의 계수 비는 정상 노화에 따라 매우 유의하게 감소하였다. 즉. 문측-전대상회/후대상회는 매 10년마다 3.1%씩 감소하였으며($P<10^{-11}$, r=0.81) 미측-전대상회/후대상회는 1.7%씩 감소하였다($P<10^{-8}$, r=0.72). 문측-전대상회와 미측-전대상회의 계수비 감소 역시 유의하였다(P<0.0005. r=0.52, 1.5%/year). 결론: 서양인 뇌에 기반한 확률뇌지도는 그동안 제한적으로만 사용되어 왔으나 한국인 뇌 표준판과 확률뇌지도 및 이 연구에서 개발한 정량화 기법은 일반에 공개하기로 하여 더욱 널리 쓰이게 되면 국내 뇌 연구 활성화에 기여할 것으로 기대 된다.

• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.295-298
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• 2008

Creatine kinase (CK; E.C. 2.7.3.2) is an important enzyme that catalyzes the reversible transfer of a phosphoryl group from ATP to creatine in energy homeostasis. The brain-type cytosolic isoform of creatine kinase (BB-CK), which is found mainly in the brain and retina, is a key enzyme in brain energy metabolism, because high-energy phosphates are transfered through the creatine kinase/phosphocreatine shuttle system. The recombinant human BB-CK protein was overexpressed as a soluble form in Escherichia coli and crystallized at $22^{\circ}C$ using PEG 4000 as a precipitant. Native X-ray diffraction data were collected to $2.2{\AA}$ resolution using synchrotron radiation. The crystals belonged to the tetragonal space group $P4_32_12$, with cell parameters of a=b=97.963, $c=164.312{\AA},\;and\;{\alpha}={\beta}={\gamma}=90^{\circ}$. The asymmetric unit contained two molecules of CK, giving a crystal volume per protein mass $(V_m)$ of $1.80{\AA}^3\;Da^{-1}$ and a solvent content of 31.6%.