• 식물병연구
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• 제10권4호
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• pp.268-271
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• 2004

작약검은뿌리썩음병은 연작을 할 경우 현저히 발생이 증가하였으며, 태백작약 품종에 비해 의성작약 품종에서 좀 더 심한 발병을 보였다. 종구로 사용하는 뇌두를 약제에 침지시 병원균은 검출되지 않았으나 물에만 침지한 경우 병원균이 검출되었다. 연작지의 토양개량시 검은뿌리썩음병의 방제는 효과가 없었으나 다조메입제로 토양을 훈증소독할 경우 토양훈증처리구에서 주근중과 근장이 단순연작구와 비교하여 50% 정도 증가하였으며 검은뿌리썩음병의 발병율은 무처리구의 경우 40.6% 정도 발병되었으나 토양훈증처리구는 4.7% 정도로 발병되어 아주 높은 방제효과를 보였으며 종구소독과 훈증처리를 병행시 발병율은 더욱 낮아졌다.

• 한국균학회지
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• 제11권2호
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• pp.99-101
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• 1983

Black root rot caused by Cylindrocladium crotalariae is one of the most serious soilborne disease in soybean. Plant height reduction of Hwangkeum Kong was 38% by the rotting of the whole main root and 9% by rotting of the half of the main root as compared with partial discoloration in the main root. Pod number per plant and seed weight were significantly reduced by the increase of the disease severity. Important yield components such as number of pods per plant and seed weight were negatively correlated with the soybean black root rot severity.

• The Plant Pathology Journal
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• 제25권1호
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• pp.1-5
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• 2009

Root rot caused by Cylindrocarpon destructans is one of the most important diseases of ginseng (Panax ginseng C. A. Meyer). Two types of symptoms found in ginseng root rot are black root rot and rusty root (rusty spots), in which disease severities are high and low, respectively. Symptom development and related histopathological changes were examined in an inoculation test on 2-year-old ginseng roots using virulent (Cy9801) and avirulent (Cy0001) isolates of C. destructans under different temperature conditions (13, 18, 23, and $28^{\circ}C$). Black root rot was only induced by Cy9801 in the lower temperature range (13, 18, and $23^{\circ}C$) and not at the higher temperature ($28^{\circ}C$). No black root rot, but only rusty spot symptoms, were induced by Cy0001 at all temperatures tested except $13^{\circ}C$, at which no symptoms occurred on over half of inoculation sites, suggesting disease development was influenced by pathogen virulence and temperature. Wound periderms were formed in all root tissues with rust spot symptoms at $28^{\circ}C$ caused by Cy9801 and at 18, 23, and $28^{\circ}C$ temperatures caused by Cy0001. No wound periderm was formed at $13^{\circ}C$ by either Cy9801 or Cy0001. Light microscopy revealed that the wound periderm was formed by initial cell divisions in cell wall formation and/or additional cell wall layering in parenchyma cells without obvious nuclear division, followed by layering of the divided cells adjacent to the inoculation sites, blocking the spread of the rot. These results suggest that disease development declined at lower temperatures and by the formation of a wound periderm at higher temperatures, and that ginseng rusty root may develop under conditions unfavorable for further disease development of C. destructans.

• 한국균학회지
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• 제8권1호
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• pp.53-57
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• 1980

An undescribed black root rot of soybean, caused by Cylindrocladium crotalariae, was observed in Suweon area. The diseased plants showed yellowing at the top and dry rot at the root. Lesions of roots and stems in the soil were red to brown and main roots were cracked. Although not observed the disease in the field, leaves of inoculated test plants in the greenhouse exhibited circular, brown lesion surrounded by chloratic halos. The fungus was recovered in culture from the infected stem and root, and the perithecia of Calonectria crotalariae were demonstrated to be present as well as the cylindrocladium state. The fungus was pathogenic to the root, stem, petioles and leaves of soybean. The probable source of primary inoculum was microsclerotia formed in infected soybean root and stem from the previous season's soybean debris. Black root rot by this fungus was considered to be one of detrimental factors to the maximum yield of soybean. From the morphological and physiological characteristics and pathogenic behaviors, this fungus was identified as Cylindrocladium(Calonectria) crotalariae.

• 한국식물병리학회지
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• 제14권3호
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• pp.209-211
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• 1998

Black dot root rot occurred severely in greenhouse tomatoes in jangseong area of Korea in April, 1996. The causal fungus of the disease was identified as Colletotrichum coccodes based on the morphological and cultural characteristics. Pathogenicity tests revealed that isolates of the fungus were responsible for the disease, and two cultivars of cherry tomato were less susceptible to the disease than other cultivars of tomato and cherry tomato tested.

• 한국균학회지
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• 제25권1호통권80호
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• pp.6-9
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• 1997

1993년 5월, 국내 여주지역의 비닐하우스포장에서 가지점뿌리썩음병이 처음 발견되었다. 이 병의 병원 진균은 형태적 및 배양적 특성에 의해 Colletotrichum coccodes (Wallr.) Mushes로 동정되었다. 이 균의 균주들을 사용하여 병원성검정을 실시한 결과, 병원성이 확인되었다.

• 농약과학회지
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• 제11권1호
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• pp.18-26
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• 2007

콩 검음뿌리썩음병의 방제를 위한 살균제 선발을 위하여 실내 및 온실 검정을 실시하였다. 한천희석법과 96-well microtiter plate법을 이용하여 총 25종의 살균제의 병원균에 대한 생육 억제 효과를 검정하였다. 보호용 살균제 중에서 iminoctadine을 제외한 dithianon, dichlofluanid, mancozeb, captan 등은 한천희석법에서 조사한 병원균의 생육을 억제하는 $EC_{50}$값은 $500{\mu}g\;mL^{-1}$ 이상으로 효과가 매우 낮았지만, 96-well microtiter plate법에서는 4.65, 0.61, 4.64, $0.29{\mu}g\;mL^{-1}$로, 병원균의 포자 발아에 대하여 매우 높은 활성을 보였다. 그러나 ergosterol 생합성을 저해하는 살균제 (EBI) 그룹은 물질에 따라 매우 다른 살균활성을 보였다. Difenconazole의 경우에는 균사생육보다는 포자발아 억제활성이 높았고, prochloraz의 경우에는 균사 생육활성과 포자 발아억제활성이 서로 유사하였다. 이에 비하여 다른 EBI 살균제들은 포자발아보다는 균사생육저해활성이 큰 것으로 나타났다. Carbendazim과 diethofencarb의 혼합제와 dazomet만이 두 가지의 실내 검정법 모두에서 우수한 효과를 보였다. Carbendazim과 diethofencarb의 혼합제를 비롯한 8종의 살균제를 선발하여 온실검정을 실시한 결과, carbendazim과 diethofencarb의 혼합제만이 병원균을 접종하기 2일 전과 후에 토양에 처리하였을 때, 75와 76.7%의 효과를 보였고, tebuconazole은 병원균을 접종하고 2일 후에 관주처리 하였을 때, 83.3%의 효과를 보였다.

• The Plant Pathology Journal
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• 제38권4호
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• pp.272-286
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• 2022

Black root rot (BRR) caused by Lasiodiplodia theobromae is an alarming disease of mulberry that causes tremendous economic losses to sericulture farmers in India and China. Successful control of this disease can be attained by screening germplasm and identifying resistant sources. Seventy four diseased root samples were collected from farmer's fields belonging to four major mulberry growing states of South India. Based on morpho-cultural and scanning electron microscopy studies, 57 fungal isolates were characterized and identified as L. theobromae. Phylogenetic analysis of concatenated internal transcribed spacer and β-tubulin sequences revealed variation of the representative 20 isolates of L. theobromae. Following the root dip method of inoculation, pathogenicity studies on susceptible mulberry genotypes (Victory-1 and Thailand male) recognized the virulent isolate MRR-142. Accordingly, MRR-142 isolate was used to evaluate resistance on a set of 45 diverse mulberry accessions. In the repeated experiments, the mulberry accession ME-0168 which is an Indonesian origin belonging to Morus latifolia was found to be highly resistant consistently against BRR. Eight accessions (G2, ME-0006, ME-0011, ME-0093, MI-0006, MI-0291, MI-0489, and MI-0501) were found to be resistant. These promising resistant resources may be exploited in mulberry breeding for developing BRR resistant varieties and to develop mapping populations which successively helps in the identification of molecular markers associated with BRR.

• International Journal of Industrial Entomology and Biomaterials
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• 제16권2호
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• pp.49-56
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• 2008

A preliminary survey on the incidence and intensity of root disease complex (association of Meloidogyne incognita and root rot pathogens) was carried out in the sericultural areas of Karnataka. A total of 280 mulberry gardens were surveyed in 14 districts of Karnataka belonging to different types of soil (red sandy, red loamy and black cotton), farming systems (irrigated and rainfed), varieties (V-1, K-2, Local and S-13) and age of the plants (0-5, 5-10 and 10-15 years). It was observed that the association of M. incognita with Botryodiplodia theobromae and Fusarium solani causes the root disease complex in mulberry. Of the 280 gardens visited, 94 were infested with the disease complex and incidence was recorded as 33.6%. The higher intensity of root disease complex was observed when the root system had more than 100 galls/plant with infection of mixed population of B. theobromae and F. solani in sandy soil under irrigated farming. The 5-10 years old mulberry plantation with V-I variety was found to be most susceptible to root disease complex. Districts like Mysore, Kolar, Mandya, Tumkur, Chitradurga and Bangalore were observed as sensitive areas. Further, the wounds caused by M. incognita in mulberry roots favour the easy entry of root rot pathogens, which increased the severity of the disease very fast.

• 식물병연구
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• 제10권1호
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• pp.8-12
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• 2004

2003년 7월, 경상북도 봉화지역의 인삼포장에서 인삼의 줄기가 쓰러지고 뿌리썩음증상을 보이는 인삼을 관찰하였다 병든 뿌리로부터 분리된 병원세균을 접종한 결과, 인삼의 잎은 황화되거나 검은색으로 변하여 시들었고, 줄기는 속이 텅비어 있었다. 뿌리는 접종부위가 썩으면서 주름이 생기고 차츰 검은색으로 변화였다. 이 병원세균은 인삼의 모든 부위에 대해 병원성이 인정되었고 이 병원세균의 형태 및 생리적 특성을 Bergey' manual에 기록된 세균의 특성과 비교한 결과, Serratia liquefaciens로 동정되었다. 이 세균은 아직 인삼의 병원세균으로 기록되어 있지 않기에 인삼뿌리썩음병으로 이 병원세균도 추가할 것을 제안한다.