• Journal of the Korean Institute of Intelligent Systems
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• v.11 no.2
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• pp.127-131
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• 2001

생체 면역시스템은 바이러스나 병원균 등의 외부 침입자로부터 자신을 보호한다. 본 논문에서는 이러한 기능을 컴퓨터 면역시스템에 적용하기 위하여 생체 면역시스템의 기본 요소인 T세포를 모델링 하였다. 모델링한 T세포는 MHC 인식부와 항원 인식부를 가지고 있으며, 매칭을 통하여 컴퓨터내의 랜덤 파일로부터 자기 파일과 비자기 파일을 구분할 수 있다. 모델링한 T세포의 유효성을 보이기 위해서 컴퓨터 바이러스의 문제에 적용하여 자기-비자기 구분이 가능함을 보인다.

• 제어로봇시스템학회:학술대회논문집
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• 2000.10a
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• pp.530-530
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• 2000

A new paradigm of technology, based on the overall interactions of technology, humans and environment through Korperlichkeit(corporeality or philosophy of Mom), is explored. Parallelism based on holism and embodiment, and relative interactions based on correspondence and interrelationships, are the key ideas in the proposed paradigm. Biological information processing systems much resemble the key ideas of interactive technology. Robots could be easily implemented from this evolutionary engineering approach.

• Proceedings of the IEEK Conference
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• 2006.06a
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• pp.799-800
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• 2006

This thesis implemented security systems of voice awareness which is higher accessible than existing security system using biological authentication system and is inexpensive in module of security device, and has an advantage in usability. Proposed the security systems of voice awareness implemented algorithm for characteristic extraction of inputted speaker's voice signal verification, and also implemented database of access control that is founded on extractible output. And a security system of voice awareness has a function of an authority of access control to system.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2006.11a
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• pp.243-246
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• 2006

본 논문에서는 모발내에 있는 약 30여가지의 생체 미네랄과 8가지의 중금속 정보 분석을 통해 생체내에 양양상태의 과잉, 결핍 및 불균형 상태를 평가하고, 그 결과가 현재 생체에 미치는 영향을 예측하여, 건강을 유지하는 방향을 제시할 수 있는 의료용 지능적 의학진단 시스템을 구축하였다. 이 논문에서는 생체내 미네랄 정보를 다중 퍼지규칙베이스 시스템으로 구축함으로써 환자에게 보다 효율적으로 치료와 예방방법을 제시할 수 있는 의학진단시스템을 구축하였다.

• Journal of Biomedical Engineering Research
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• v.30 no.1
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• pp.1-9
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• 2009

Many types of small animal imaging modalities, like micro-CT, micro-PET, and micro-SPECT, have been recently developed worldwide. Small animal imaging systems are now recognized as indispensable tools to validate efficacy and safety of new drugs or new therapeutic methods using the animal disease models. With increasing demands for small animal imaging in biomedical research, multimodal small animal imaging systems, like micro-PET/CT or micro PET/MRI, are now also being developed. Small animal imaging with spatial resolution and sensitivity comparable to human imaging is quite challenging since laboratory small animals are much smaller than human beings. Research activities in Korea on small animal imaging systems are reviewed in this paper. In the field of micro-CT and micro-PET, many world-class technologies have been developed successfully in Korea. It is expected that the developed animal imaging system technologies can be used in the development of clinical imaging systems in Korea in the near future.

• BMB Reports
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• v.44 no.4
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• pp.250-255
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• 2011

In multicellular organisms, including humans, understanding expression specificity at the tissue level is essential for interpreting protein function, such as tissue differentiation. We developed a prediction approach via generated sequence features from overrepresented patterns in housekeeping (HK) and tissue-specific (TS) genes to classify TS expression in humans. Using TS domains and transcriptional factor binding sites (TFBSs), sequence characteristics were used as indices of expressed tissues in a Random Forest algorithm by scoring exclusive patterns considering the biological intuition; TFBSs regulate gene expression, and the domains reflect the functional specificity of a TS gene. Our proposed approach displayed better performance than previous attempts and was validated using computational and experimental methods.

• BMB Reports
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• v.45 no.4
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• pp.213-220
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• 2012

Viruses have evolved to manipulate the host cell machinery for virus propagation, in part by interfering with the host cellular signaling network. Molecular studies of individual pathways have uncovered many viral host-protein targets; however, it is difficult to predict how viral perturbations will affect the signaling network as a whole. Systems biology approaches rely on multivariate, context-dependent measurements and computational analysis to elucidate how viral infection alters host cell signaling at a network level. Here we describe recent advances in systems analyses of signaling networks in both viral and non-viral biological contexts. These approaches have the potential to uncover virus- mediated changes to host signaling networks, suggest new therapeutic strategies, and assess how cell-to-cell variability affects host responses to infection. We argue that systems approaches will both improve understanding of how individual virus-host protein interactions fit into the progression of viral pathogenesis and help to identify novel therapeutic targets.

• Korean Journal of Agricultural Science
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• v.43 no.2
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• pp.163-175
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• 2016

For the past decades, advances in computational devices have propelled mathematical modeling to become an effective tool for solving the black box of complex biological systems because of its prominent analytical power and comprehensive insight. Nevertheless, modeling is still limitedly used in the fields of agriculture and food which generally concentrate on producing experimental data rather than processing them. This study, hence, intends to introduce modeling in terms of its procedure types of structure, formulation, analyses, and software, with reviews of current notable studies from micro to macro scales so as to propose the modeling technique as a novel approach in discerning conundrums in agriculture and food systems. We expect this review to provide an eligible source for researchers who are willing to apply modeling techniques into the unexplored fields related to bio-systems that comprehensively include biology, nutrition, agriculture, food, animal science, and ecology.

• Toxicological Research
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• v.32 no.1
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• pp.5-8
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• 2016

Risk assessment is the process of quantifying the probability of a harmful effect to individuals or populations from human activities. Mechanistic approaches to risk assessment have been generally referred to as systems toxicology. Systems toxicology makes use of advanced analytical and computational tools to integrate classical toxicology and quantitative analysis of large networks of molecular and functional changes occurring across multiple levels of biological organization. Three presentations including two case studies involving both in vitro and in vivo approaches described the current state of systems toxicology and the potential for its future application in chemical risk assessment.

• Journal of Microbiology and Biotechnology
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• v.13 no.6
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• pp.926-936
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• 2003

Overexpression of human Bcl-2 protein in recombinant Chinese hamster ovary (rCHO) cells producing humanized antibody (SH2-0.32) considerably suppressed sodium butyrate (NaBu)-induced apoptosis during batch culture by using commercially available serum-free medium, which extended the culture longevity. Due to the extended culture longevity provided by the anti-apoptotic effect of Bcl-2 overexpression, the final antibody concentration of 14C6-bcl-2 culture (Bcl-2 high producer, $23\;\mu\textrm{g}\;ml^{-1}$) was 2 times higher than that of the $SH2-0.32-{\Delta}bcl-2$ culture (cells transfected with bcl-2-deficient plasmid, $10.5\;\mu\textrm{g}\;ml^{-1}$) in the presence of NaBu. To determine the effect of NaBu/Bcl-2 overexpression on the molecular integrity of protein products, antibodies purified from 14C6-bcl-2 and $SH2-0.32-{\Delta}bcl-2$ cultures in the presence of NaBu were characterized by using various molecular assay systems. For comparison, antibody purified from the parental rCHO cell culture (SH2-0.32) in the absence of NaBu was also characterized. No significant changes in molecular weight of antibodies could be observed by SDS-PAGE. From GlycoSep-N column analysis, it was found that the core oligosaccharide structure ($GlcNAc_2Man_3GlcNAc_2$) was not affected by NaBu/Bcl-2 overexpression, while the microheterogeneity of N-linked oligosaccharide structure was slightly affected. Compared with the antibody produced in the absence of NaBu, the proportion of neutral oligosaccharides was increased from 10% (14C6-bcl-2) to 16% ($SH2-0.32-{\Delta}bcl-2$) in the presence of NaBu, which was accompanied by the reduced proportion of acidic oligosaccharides, especially of monosialylated and disialylated forms. The changes in microheterogeneous oligoformal structures of antibody in turn affected the mobility of antibody isoforms in isoelectric focusing (IEF), resulting in the occurrence of some more basic antibody isoforms produced in the presence of NaBu. However, the antigen-antibody binding properties were not changed by alteration of glycosylation pattern. The competitive enzyme-linked immunosorbent assay (ELISA) showed that the antibody produced by NaBu/Bcl-2 overexpression maintained its antigen-antibody binding properties with binding affinity of about $2.5{\times}10^9{\;}M^{-1}$. Taken together, no significant effects of NaBu/Bcl-2 overexpression on the molecular integrity of antibodies, produced by using serum-free medium, could be observed by the molecular assay systems.