• Title/Summary/Keyword: Biological degradation

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Biological Decolorization Characteristics of Dyeing Wastewater (염색폐수의 생물학적 색도제거 연구)

  • Kim, Mee-Kyung;Seo, Sang-Jun;Shin, Eung-Bai
    • Journal of Korean Society on Water Environment
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    • v.22 no.2
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    • pp.333-341
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    • 2006
  • The concept for the decolorization in biological dye wastewater treatment systems is based on anaerobic treatment, for the reductive cleavage of the dyes' azo linkages, in combination with aerobic treatment, for the degradation of the products from azo dye cleavage, aromatic amines. Batch tests were conducted to examine the conditions and the factors affecting biological treatment of dye wastewater. From the tests, the removal efficiencies of organics and colors of dyeing wastewater were improved to $COD_{Cr}$ 27% and color 9% by injecting 10% of the domestic wastewater as a cosubstrate, and $COD_{Cr}$ 30%, color 22% with 30% injection of domestic wastewater. Therefore it was proved that decolorization efficiency is demonstrated with domestic wastewater as a cosubstrate. The analysis of aromatic amines in wastewater showed that decolorization was achieved by cometabolism while aromatic amines were produced by cleavage of azo bonds under anaerobic conditions and these products were removed in an aerobic tank subsequently.

The Study of Iodine Metabolism IN VIVO Utilizing I-131 (방사선 동위원소 I-131을 이용한 요드의 IN VIVO 대사 연구)

  • Byun, Si-Myung
    • Applied Biological Chemistry
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    • v.19 no.2
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    • pp.70-74
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    • 1976
  • In order to study the mechanism of biosynthesis of thyroid hormones, radioactive iodine was injected into the rats and thyroid glands were removed. Iodine compounds hydrolyzed by pancreatin viokase were separated by paper chromatography and analyzed by radioautography. Radioautograms showed that the uptake of iodine starts immediately and forms diiodotyrosine through monoiodotyrosine. Evidence supported the possibility that diiodotyrosine is a precursor of thyrosine and triiodothyronine is a degradation product of thyroxine. The rat administered propylthiouracil showed inorganic iodine concentration activity, while the binding activity was prevented.

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Quorum Sensing and Quorum-Quenching Enzymes

  • Dong, Yi-Hu;Zhang, Lian-Hui
    • Journal of Microbiology
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    • v.43 no.spc1
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    • pp.101-109
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    • 2005
  • To gain maximal benefit in a competitive environment, single-celled bacteria have adopted a community genetic regulatory mechanism, known as quorum sensing (QS). Many bacteria use QS signaling systems to synchronize target gene expression and coordinate biological activities among a local population. N-acylhomoserine lactones (AHLs) are one family of the well-characterized QS signals in Gram-negative bacteria, which regulate a range of important biological functions, including virulence and biofilm formation. Several groups of AHL-degradation enzymes have recently been identified in a range of living organisms, including bacteria and eukaryotes. Expression of these enzymes in AHL-dependent pathogens and transgenic plants efficiently quenches the microbial QS signaling and blocks pathogenic infections. Discovery of these novel quorum quenching enzymes has not only provided a promising means to control bacterial infections, but also presents new challenges to investigate their roles in host organisms and their potential impacts on ecosystems.

Enhanced Production of Fatty Acids via Redirection of Carbon Flux in Marine Microalga Tetraselmis sp.

  • Han, Mi-Ae;Hong, Seong-Joo;Kim, Z-Hun;Cho, Byung-Kwan;Lee, Hookeun;Choi, Hyung-Kyoon;Lee, Choul-Gyun
    • Journal of Microbiology and Biotechnology
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    • v.28 no.2
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    • pp.267-274
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    • 2018
  • Lipids in microalgae are energy-rich compounds and considered as an attractive feedstock for biodiesel production. To redirect carbon flux from competing pathways to the fatty acid synthesis pathway of Tetraselmis sp., we used three types of chemical inhibitors that can block the starch synthesis pathway or photorespiration, under nitrogen-sufficient and nitrogen-deficient conditions. The starch synthesis pathway in chloroplasts and the cytosol can be inhibited by 3-(3,4-dichlorophenyl)-1,1-dimethylurea and 1,2-cyclohexane diamine tetraacetic acid (CDTA), respectively. Degradation of glycine into ammonia during photorespiration was blocked by aminooxyacetate (AOA) to maintain biomass concentration. Inhibition of starch synthesis pathways in the cytosol by CDTA increased fatty acid productivity by 27% under nitrogen deficiency, whereas the blocking of photorespiration in mitochondria by AOA was increased by 35% under nitrogen-sufficient conditions. The results of this study indicate that blocking starch or photorespiration pathways may redirect the carbon flux to fatty acid synthesis.

Cloning and Sequence Analysis of Two Catechol-degrading Gene Clusters from a Phenol-utilizing Bacterium Pseudomonas putida SM25

  • Jung, Young-Hee;Ka, Jong-Ok;Cheon, Choong-Ⅰll;Lee, Myeong-Sok;Song, Eun-Sook;Daeho Cho;Park, Sang-Ho;Ha, Kwon-Soo;Park, Young-Mok
    • Journal of Microbiology
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    • v.41 no.2
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    • pp.102-108
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    • 2003
  • A 6.1 kb Sph I fragment from the genomic DNA of Pseudomonas putida SM 25 was cloned into the veetor pUC19. The open reading frame of catB was found to consist of 1,122 nucleotides. The sequence alignment of the catB gene products from different kinds of bacteria revealed an overall identity ranging from 40 to 98%. The catC gene contained an open reading frame of 96 codons, from which a protein with a molecular mass of about 10.6 kDa was predicted. The amino acids in the proposed activesite region of CatC were found to be almost conserved, including the charged residues. Since the catBC genes in P. putida SM25 were tightly linked, the could be regulated under coordinate transcription, and transcribed from a single promoter located upstream of the catB gene, as in P. putida RBI.

Biological Removal of Nitrogen Oxides from Combustion Flue Gases (연소배가스 중 질소산화물(NOx) 제거를 위한 생물학적 기술)

  • Lee, Ki-Say
    • Applied Chemistry for Engineering
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    • v.21 no.3
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    • pp.243-251
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    • 2010
  • Nitrogen oxides (NOx) in combustion flue gas are currently mitigated by chemical processes such as catalytic reduction, absorption and adsorption. However, development of environmentally sustainable biological processes is necessary in the near future. In this paper, the up-to-dated R&D trend of biological methodologies regarding NOx removal was reviewed, and their advantages and disadvantages were discussed. The principles and applications of bacterial system including nitrification and denitrification and photosynthetic microalgae system were compared. In order to enhance biological treatment rate and performance, the insoluble nitric oxide (NO) should be first absorbed using a proper solubilization agent, and then microbial degradation or fixation is to be followed. The use of microalgal system has a good prospect because it can fix $CO_2$ and NOx simultaneously and requires no additional carbon for energy source.

Surfactant Selection for the Enhanced Biological Degradation of Toluene (Toluene의 생물학적 분해능 향상을 위한 계면활성제의 선정)

  • Kim Yong-Sik;Son Young-Gyu;Khim Jee-Hyeong;Song Ji-Hyeon
    • Journal of Soil and Groundwater Environment
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    • v.10 no.4
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    • pp.26-32
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    • 2005
  • Surfactants can be used to enhance the mass transfer rate of hydrophobic compounds into the biologically active liquid phase, resulting in an increase in biodegradation rate of toluene. In this study, the mass transfer rate and the biocompatibility of toluene in the presence of various surfactants were evaluated. Four anionic and non ionic surfactants were tested: sodium dodecyl sulfate (SOS), TritonX-100, Tween 80, and BYK-345 (silicone surfactant). Experimental results showed that BYK-345 at the critical micelle concentration (CMC) enhanced the solubility of toluene. However, there was no increase in the solubility of toluene by SOS and TritonX-100 at their CMCs. With the addition of each surfactant into deionized water the mass transfer rate became faster than that of the case with no surfactant. A bottle study using toluene-degrading microorganisms showed that SOS seriously reduced toluene removal presumably due to the toxicity of the anionic surfactant and/or the substrate competition between the surfactant and toluene. In addition, the degradation rate of toluene was decreased in the presence of BYK-345, indicating that BYK-345 adversely affects the activity of microorganisms. However, TritonX-100 and Tween 80 did not decrease the degradation rate of toluene significantly. Rather, at the low concentration of TritonX-100 toluene degradation rate was even increased. Overall the experimental results suggest that TritonX-100 be the appropriate surfactant for enhanced biological degradation of toluene.

Biological Characteristics of Anodic Electrolyzed Water (산성전리수의 생물학적 특성)

  • 김윤경;민병술;민중기;이종권;이윤배;류근걸;이미영
    • Korean Journal of Environmental Biology
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    • v.22 no.2
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    • pp.265-272
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    • 2004
  • Biological characteristics of anodic electrolyzed water were investigated in this study. Linear DNAs which were incubated at $4^\circ{C}$ and $25^\circ{C}$ for 10 mins in the anodic electrolyzed water were degraded about 40% and 50%, respectively. But the DNA was amplified pretty well without any degradation through polymerase chain reaction in the presence of anodic electrolyzed water. Protein degradation hardly occurred in the distilled water during entire incubation time of 7 days, while protein began to be degraded from 4 days in the anodic electrolyzed water. Rice seeds could germinate in the distilled water and anodic electrolyzed water with the same germination ratio, however, the anodic electrolyzed water inhibited the growth of roots and total length of rice seedlings in the soil. Anodic electrolyzed water did not affect the growth curve and cell number of marine alga significantly. The anodic electrolyzed water inhibited the browning of potato by inactivating 50% of polyphenol oxidase activity.

Research Trend of The Heat-Treatment of Wood for Improvement of Dimensional Stability and Resistance to Biological Degradation (목재의 치수안정성과 내후성 개선을 위한 열처리 가공에 관한 연구 동향)

  • Kim, Yeong-Suk
    • Journal of the Korean Wood Science and Technology
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    • v.44 no.3
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    • pp.457-476
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    • 2016
  • This was investigated on the major issues and research trends regarding the heat-treatment of woods through literature reviews. The principal heat-treatment technologies utilized for industrial purposes include the Plato-process (Netherlands), the Retification process (France), the OHT-process (Germany), and the Thermowood Process (Finland). Factors that mainly influence the heat-treatment process are the wood species, process temperature, processing time, and the heating medium (air, steam, vacuum, N2, oil, etc.). Researches on investigating the optimal conditions with these process conditions being the variables stand as the mainstream. Heat-treated woods present dimensional stability improvement, but mass loss and strength reduction, a wide variations for decaying inhibition, and insufficient resistance against mold, wood borer, and termites. For further improvement in respects of durability or resistance to biological degradation, necessity to search for more suitable heat treatment process and processing conditions fit for each wood species has been suggested. Exploiting new ways to utilize heat-treated wood and extending its range of use have been considered to be important matters that need more effort put into for the sustainable and sound environment as well as saving the wood resources.

Flavor Characteristics of Hanwoo Beef in Comparison with Other Korean Foods

  • Ba, Hoa Van;Ryu, Kyeong-Seon;Hwang, In-Ho
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.3
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    • pp.435-446
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    • 2012
  • The present study identified volatile flavor components of Hanwoo longissimus muscle and other Korean foods (Doenjang, Chungukjang, sesame oil) and their traits were compared in relation with flavor precursors that include fatty acids and protein degradation products. Hanwoo longissimus muscle was purchased from a commercial abattoir while the other foods were sampled from three separate households. The results showed totals of 68 ($9.94{\mu}g/g$), 60 ($15.75{\mu}g/g$), 49 ($107.61{\mu}g/ml$) and 50 ($7.20{\mu}g/g$) volatile components for Doenjang, Chungukjang, sesame oil and Hanwoo beef longissimus, respectively (p<0.05). Aldehydes were the most predominant components in beef, but alcohols, acids and esters, and pyrazines are probably the major contributors to the flavor characteristics of other foods. SDS-PAGE revealed that beef longissimus muscle and Doenjang showed higher protein degradation than other foods which could be likely related to chiller ageing and ripening process. The total polyunsaturated fatty acids were approximately 50, 60, 41 and 5% for Doenjang, Chungukjang, sesame oil and beef longissimus muscle, respectively. Based on the mechanism(s) of generation of the volatile compounds and the chemical composition of each food sample, differences and traits of volatile flavor components among the four food types are likely due to fatty acid profiles, proteolytic activity and processing conditions. Aroma intense compounds like pyrazines and sulfur-containing compounds were limited in cooked beef in the current experimental condition (i.e., relatively low heating temperature). This suggests that higher heating temperature as in the case of roasting is needed for the generation of high aroma notes in meat. Furthermore, proteolytic activity and stability of fatty acids during ageing have a great influence on the generation of flavor components in cooked beef.