• 제목/요약/키워드: Biological Engineering

검색결과 9,444건 처리시간 0.031초

Growth Prpmotion of Taxus brevifolia Cell Suspension Culture Using Conditioned Medium

  • Kim, Myung-Hwan;Chun, Su-Mwan;Kim, Dong-Il
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • 제5권5호
    • /
    • pp.350-354
    • /
    • 2000
  • The growth promotion of a Taxus brevifolia cell suspension culture was investigated using conditioning factors. The conditioning factors produced and secreted from cultured cells usually stimulate cell division and the production of secondary metabolites. Therefore, the effective incubation time for the optimal secretion of conditioning factors was firstly determined for the promotion of cell growth. Conditioned media obtained by cultivating for 2 and 5 days showed the promotion of initial cell growth during the early cell growth period. However, the positive effect of the conditioning factors on the initial cell growth did not continue because of the depletion of the medium nutrients. Accordingly, the addition of a carbon source to the conditioned medium prolonged the positive effect on the cell growth. The addition of sucrose to the conditioned medium resulted in the maximum cell density being reached 4 days earlier compared to the control group and an increased substrate yield.

  • PDF

Evolutionary Signature of Information Transfer Complexity in Cellular Membrane Proteomes

  • Kim, Jong-Min;Kim, Byung-Gee;Oh, S.-June
    • Genomics & Informatics
    • /
    • 제7권2호
    • /
    • pp.111-121
    • /
    • 2009
  • Cell membrane proteins play crucial roles in the cell's molecular interaction with its environment and within itself. They consist of membrane-bound proteins and many types of transmembrane (TM) proteins such as receptors, transporters, channel proteins, and enzymes. Membrane proteomes of cellular organisms reveal some characteristics in their global topological distribution according to their evolutionary positions, and show their own information transfer complexity. Predicted transmembrane segments (TMSs) in membrane proteomes with HMMTOP showed near power-law distribution and frequency characteristics in 6-TMS and 7-TMS proteins in prokaryotes and eukaryotes, respectively. This reaffirms the important roles of membrane receptors in cellular communication and biological evolutionary history.

Enhanced Production of 1,2-Propanediol by tpil Deletion in Saccharomyces cerevisiae

  • Jung, Joon-Young;Choi, Eun-Sil;Oh, Min-Kyu
    • Journal of Microbiology and Biotechnology
    • /
    • 제18권11호
    • /
    • pp.1797-1802
    • /
    • 2008
  • Saccharomyces cerevisiae was metabolically engineered to improve 1,2-propanediol production. Deletion of the tpil (triosephosphate isomerase) gene in S. cerevisiae increased the carbon flux to DHAP (dihydroxylacetone phosphate) in glycolysis, resulting in increased glycerol production. Then, the mgs and gldA genes, the products of which convert DHAP to l,2-propanediol, were introduced to the tpil-deficient strain using a multicopy plasmid. As expected, the intracellular level of methylglyoxal was increased by introduction of the mgs gene in S. cerevisiae and that of 1,2-propanediol by introduction of both the mgs and gldA genes. As a result, 1.11 g/l of 1,2-propanediol was achieved in flask culture.

Study of Operation Condition of Biofilter Using Fibril-form Matrix for Odor Gas Removal (악취가스 제거를 위안 섬유상 담체를 적용한 바이오필터 운전조건에 관한 연구)

  • Jeong Gwi-Taek;Lee Gwang-Yeon;Byun Ki-Young;Lee Kyoung-Min;Sunwoo Chang-Shin;Lee Woo-Tae;Park Chan-Young;Kim Do-Hyeong;Cha Jin-Myoung;Jang Young-Seon;Park Don-Hee
    • KSBB Journal
    • /
    • 제20권5호
    • /
    • pp.341-344
    • /
    • 2005
  • This research was performed for developing of biological treatment process of odor gas such as MEK, $H_2S$, and toluene, which was generated from the food waste recycling process. To establish the operational conditions of odor gas removal by small-scale biofiltration equipment, it was continuously operated by using toluene as a treating odor object. When the odor treating microorganisms were adhered to fibril form biofilter, high removal efficiency over $93\%$ was obtained by biofilm formation. At 400 ppm of inlet odor gas concentration and 10 sec of retention time, the removal efficiency was $76\%$ and $93\%$ in 1 st stage reactor and End stage reactor, respectively. However, the removal efficiency remained over $97\%$ at the operational conditions above 15 sec of retention time.

Evaluation of Biohydrogen Production Using Various Inoculum Sources (다양한 접종원을 이용한 바이오수소 생산 평가)

  • Geumhee, Kim;Jiho, Lee;Hyoju, Yang;Yun-Yeong, Lee;Yoonyong, Yang;Sungho, Choi;Moonsuk, Hur;Byounghee, Lee;Kyung-Suk, Cho
    • Microbiology and Biotechnology Letters
    • /
    • 제50권4호
    • /
    • pp.557-562
    • /
    • 2022
  • In this study we evaluated biohydrogen production potential as operational parameters (substrate, salt concentration, and temperature) using eight inoculum sources. While the volumetric biohydrogen production rate was significantly affected by temperature and inoculum sources, substrate and salt concentration did not have a significant effect on the biohydrogen production. Mesophilic temperature (37℃) was also found more appropriate for the hydrogen production than thermophilic temperature (50℃). Rate, while the eight inoculum sources, anaerobic digestion sludge exhibited the fastest biohydrogen production. The maximum production rate from anaerobic digestion sludge was 2,729 and 1,385 ml-H2·l-1·d-1 at mesophilic and thermophilic temperature, respectively.

Mechanical and Biological Characteristics of Reinforced 3D Printing Filament Composites with Agricultural By-product

  • Kim, Hye-Been;Seo, Yu-Ri;Chang, Kyeong-Je;Park, Sang-Bae;Seonwoo, Hoon;Kim, Jin-Woo;Kim, Jangho;Lim, Ki-Taek
    • Food Engineering Progress
    • /
    • 제21권3호
    • /
    • pp.233-241
    • /
    • 2017
  • Scaffolds of cell substrates are biophysical platforms for cell attachment, proliferation, and differentiation. They ultimately play a leading-edge role in the regeneration of tissues. Recent studies have shown the potential of bioactive scaffolds (i.e., osteo-inductive) through 3D printing. In this study, rice bran-derived biocomposite was fabricated for fused deposition modeling (FDM)-based 3D printing as a potential bone-graft analogue. Rice bran by-product was blended with poly caprolactone (PCL), a synthetic commercial biodegradable polymer. An extruder with extrusion process molding was adopted to manufacture the newly blended "green material." Processing conditions affected the performance of these blends. Bio-filament composite was characterized using field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray spectroscopy (EDX). Mechanical characterization of bio-filament composite was carried out to determine stress-strain and compressive strength. Biological behaviors of bio-filament composites were also investigated by assessing cell cytotoxicity and water contact angle. EDX results of bio-filament composites indicated the presence of organic compounds. These bio-filament composites were found to have higher tensile strength than conventional PCL filament. They exhibited positive response in cytotoxicity. Biological analysis revealed better compatibility of r-PCL with rice bran. Such rice bran blended bio-filament composite was found to have higher elongation and strength compared to control PCL.

Effect of Microbubble Ozonation Process on Performance of Biological Reactor System for Excess Sludge Solubilisation (마이크로버블오존을 이용한 잉여슬러지 가용화 처리가 생물반응조의 성능에 미치는 영향)

  • Lee, Shun-Hwa;Jung, Kye-Ju;Kwon, Jin-Ha;Lee, Se-Han
    • Journal of Korean Society of Environmental Engineers
    • /
    • 제33권2호
    • /
    • pp.113-119
    • /
    • 2011
  • The study purpose was to examine an effect of zero emission of excess sludge on biological reactor and treated water quality within the biological reactor in the process of biological treatment combined with excess sludge reduction system with ozone. Under an ozone injection rate 0.03 g $O_3/g$ SS, Sludge Disintegration Number (SDN) 3 and less than pH 4 as pre-treatment process, it was possible to maintain a stable biological treatment process without sludge disintegration. In the test of $OUR_{max}$, of sludge, its value was hardly under the condition of ozone injection rate 0.03 g $O_3/g$ SS. There were almost no changes of MLVSS/MLSS within biological reactor followed by a solubilization of excess sludge. Accumulation of microorganism within biological reactor was also not observed. After solubilization of excess sludge, an increase for organic matter and SS concentrations of an effluent was not observed and T-N concentration was reduced by increasing nitrification and denitrification rate within biological reactor. Most of T-P was not removed by zero emission of excess sludge and was leaked by being included in effluents.

Removal of Metal Ions(Cd) from Aqueous Solution by Pseudomonas sp.

  • Jeong, Jeong-Hwa;Kim, Dong-Hyun;Kong, Sung-Ho;Lim, Eun-Jin;Lee, Jong-Yeol;Bae, Young-Su;Lee, Sang-Seob
    • Proceedings of the Microbiological Society of Korea Conference
    • /
    • 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
    • /
    • pp.195.2-195
    • /
    • 2005
  • PDF

Real-time Detection of Magnetic Beads using Highly Sensitive Spin-valve Devices for a Chip-cytometer

  • Roh, Jong-Wook;Son, Oh-Taek;Jung, Hyo-Il;Lee, Woo-Young
    • Proceedings of the Korean Magnestics Society Conference
    • /
    • 한국자기학회 2010년도 임시총회 및 하계학술연구발표회
    • /
    • pp.189-190
    • /
    • 2010
  • Our results demonstrate the possibility of implementing a chip-cytometer for biological applications using high-sensitive spin-valve devices integrated with a microfluidic channel. Further studies will be extended to the real-time detection of animal cells coated with magnetic beads for the biological applications.

  • PDF