• Korean Chemical Engineering Research
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• v.60 no.3
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• pp.392-397
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• 2022

Methane is one of the major greenhouse gases, recently, the biotechnological conversion from methane to high-value added chemicals have emerged as an effort to reduce methane gas emission. In this study, we optimized ectoine bio-milking conditions in which cells were repeatedly used to improve intracellular and extracellular ectoine yield from methane by using Methylomicrobium alcaliphilum 20ZDP2. First, the cultivation and intracellular ectoine accumulation conditions were optimized with respect to the growth phase and medium salinity to achieve the highest yield of synthesis. Second, ectoine excretion was optimized by determining the ectoine secretion time (15 min) in appropriate medium salinity under hypoosmotic conditions (1% NaCl). Finally, bio-milking of ectoine was successfully repeated more than 10 times using M. alcaliphilum 20ZDP2, and the ectoine yield was improved up to 129.29 mg/ DCW g.

• Journal of Microbiology and Biotechnology
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• v.29 no.7
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• pp.1043-1052
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• 2019

Active lipase-producing bacterium Burkholderia gladioli Bps-1 was rapidly isolated using a modified trypan blue and tetracycline, ampicillin plate. The electro-phoretically pure enzyme was obtained by purification using ethanol precipitation, ion-exchange chromatography, and gel filtration chromatography. The molecular weight was 34.6 kDa and the specific activity was determined to be 443.9 U/mg. The purified lipase showed the highest activity after hydrolysis with $p-NPC_{16}$ at a pH of 8.5 and $50^{\circ}C$, and the $K_m$, $k_{cat}$, and $k_{cat}/K_m$ values were 1.05 mM, $292.95s^{-1}$ and $279s^{-1}mM^{-1}$, respectively. The lipase was highly stable at $7.5{\leq}pH{\leq}10.0$. $K^+$ and $Na^+$ exerted activation effects on the lipase which had favorable tolerance to short-chain alcohols with its residual enzyme activity being 110% after being maintained in 30% ethanol for 1 h. The results demonstrated that the lipase produced by the strain B. gladioli Bps-1 has high enzyme activity and is an alkaline lipase. The lipase has promising chemical properties for a range of applications in the food-processing and detergent industries, and has particularly high potential for use in the manufacture of biodiesel.

• Membrane Journal
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• v.23 no.6
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• pp.432-438
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• 2013

Mixed-matrix membranes (MMMs) containing MIL-100(Fe), a MOF type, were fabricated in this study. MMMs up to 30 wt% MOF loading were prepared, and their gas permeabilities were tested. $H_2$, $CO_2$, $O_2$, $N_2$, and $CH_4$ gas permeabilities increased with the MOF loading, while $SF_6$, the largest kinetic diameter in this study, exhibited reduction of gas permeability with the loading. Ideal gas selectivity of $N_2/SF_6$ improved by 40% as compared with pure polyimide membrane, suggesting the proposed MMMs were suitable for $N_2/SF_6$ separation.

• Applied Chemistry for Engineering
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• v.24 no.3
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• pp.285-290
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• 2013

Recently a novel bio refinery process with using nonedible biomass, especially microalgae, has been developed in order to directly reduce $CO_2$ concentration from flue gas and simultaneously produce renewable bio fuel. Micro algae-to-biofuel processes are composed of microalgae cultivation, harvesting, lipid extraction, and bio fuel conversion. So, there are concerns about the energy efficiencies of bio refinery processes. In this study, the net energy ratio of microalgae processes were calculated for the microalgae produced from a pilot photobioreacto using $CO_2$ released from coal combustion. In this study, trans-esterification and pyrolysis processes were used to analyze the net energy efficiencies. Micro algae-to-biofuel processes might produce bio fuels with the higher energy than that of the total consumed energy for cultivation, harvesting, extraction and conversion. If the lipid content of microalgae was higher, the trans-esterification conversion process was more effective than that of pyrolysis process.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1996.05a
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• pp.63-73
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• 1996

The importance of plant factory which blend agriculture and manufacture has been discussed widely. This company has researched hydroponics technique for cultivation which has been mainly developed in the sites of Okinawa and Hyougo Prefecture. In 1987, we stopped the previous research there, and started a new one far plant factory at the Agricultural Research Center of Chiba Prefecture. In 1989, we built four experimental plant factories (120㎡) at Chiba Oil Factory Refinery in Ichihara-city, developing techniques of cultivation, equipment production and environmental control for cultivation system of tomato, strawberry and leaf vegetables. (omitted)

• 식품문화 한맛한얼
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• v.1 no.4
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• pp.97-101
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• 2008

바이오과학기술(BT)과 연관된 첨단기술 용어로 '클린 에너지', '차세대 에너지원', '바이오 연료 에탄올', '에너지 농장' 등의 말이 경쟁하듯 등장하고 있다. 원유를 대신 할 연료를 생물자원에서 얻는다고 하여 '바이오 유전개발'이라는 말도 쓰인다. 바이오 자원을 이용하여 에탄올을 생산하려는 바이오 정유공장(Bio refinery plant) 사업은 유전개발 못지 않은 미래 산업으로 대두되고 있다. 한국의 몇몇 기업은 바이오 정유공장의 원료로 사용할 뿌리작물 카사바를 열대지역 제3국가에서 대량 재배하려는 계획을 추진하고 있다.

• Journal of the Korean Society for Precision Engineering
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• v.28 no.2
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• pp.133-139
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• 2011

We have performed optical design of light guide plates (LGPs) for a photobioreactor by using an illumination design tool. An LGP having light emitting diodes (LEDs) as a light source on the four sides was designed by optimizing the distribution of LGP patterns and design of another LGP, on top of which sunlight was focused by a Fresnel lens, was executed. Finally, a hybrid-solar-and-LED-lighting scheme was described. Detailed design process and optical performances of the designed LGPs are presented.

• Mycobiology
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• v.38 no.2
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• pp.108-112
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• 2010

To investigate the possible roles of LAMMER kinase homologue, Lkh1, in Schizosaccharomyces pombe, whole proteins were extracted from wild type and lkh1-deletion mutant cells and subjected to polyacrylamide gel electrophoresis. Differentially expressed proteins were identified by tandem mass spectrometry (MS/MS) and were compared with a protein database. In whole-cell extracts, 10 proteins were up-regulated and 9 proteins were down-regulated in the mutant. In extracellular preparations, 6 proteins were up-regulated in the lkh1+ null mutant and 4 proteins successfully identified: glycolipid anchored surface precursor, $\beta$-glucosidase (Psu1), cell surface protein, glucan 1,3-$\beta$-glucosidase (Bgl2), and exo-1,3 $\beta$-glucanase (Exg1). These results suggest that Lkh1 is involved in regulating cell wall assembly.

• Journal of the Korean Society for Precision Engineering
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• v.28 no.2
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• pp.125-132
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• 2011

This paper describes current status and future prospects of the mass production of microalgae biomass. Microalgae have attracted considerable attention since they not only effectively fix $CO_2$ gas during their metabolic process but also have the great potential to be utilized for producing valuable substances as a kind of efficient light-harvesting cell factories. In this review, we outline various types of photobioreactors employed for mass production of biomass by culturing microalgae in a well controlled way and give an overview about the present state of affairs, both domestic and international, in the field of the microalgal culturing technologies.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.4
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• pp.662-666
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• 2011

In this study, distribution characteristics of dioxins in soils in Busan, Korea were investigated regarding different land use types. Soil sampling sites that distributed through the Busan city showed dioxin concentration ranging from 0.489 to $322.736pg-TEQ\;g^{-1}$ dry weight with a mean value of $26.257pg-TEQ\;g^{-1}$ dry weight. The mean dioxin concentrations of investigated soils ranged from 1.554 to $50.357pg-TEQ\;g^{-1}$ dry weight in consideration of each land use type. That in metal refinery sites with $50.357pg-TEQ\;g^{-1}$ dry weight was higher than any other sites, followed by waste incinerator sites with $44.285pg-TEQ\;g^{-1}$ dry weight. The majority of soil samples had the same dioxin congener profiling despite the different range of dioxin concentration. Octa-CDD was the major contributor among seventeen dioxin congeners with the range from 29.5 to 70.1% in real values. In contrast to real values, dioxin congener profiles in TEQ values were dominated by 2,3,4,7,8-PeCDF which contributed about 35.3~43.8% to the total dioxin concentrations. It was judged by these results that penta-CDF was the major contributor of soil samples in Busan city. The mean ratio of PCDFs/PCDDs in real values was about 0.71, but that in TEQ values was, in contrast to it, approximately 3.03.