• 생명과학회지
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• 제20권7호
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• pp.1086-1092
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• 2010

본 연구는 토종닭 순계(적갈계통, 황갈계통), 토종닭 실용계와 오골계 및 외래품종(Hy-Line Brown: HB, White Leghorn: WL)을 대상으로 13종의 MS marker (ADL0309, ADL181, ADL190, ADL279, LEI0073, LEI0192, MCW083, MCW120, MCW153, MCW214, MCW217, MCW226, MCW322)을 활용하여 집단 및 품종간의 유전적 다양성을 분석 하였다. 13종의 MS marker 내에서 총 120개의 대립유전자를 확인 하였으며 평균 9.2개의 대립유전자를 보유한 것으로 나타났다. 관측된 이형질성, 기대되는 이형질성 및 PIC의 평균값은 각각 0.63, 0.72 그리고 0.678로 확인되었다. 가장 많은 평균대립유전자를 보유한 집단은 토종닭 실용계집단이 5.9로 확인 되었으며 기대되는 이형질성이 0.629로 비교적 높게 나타났다. 이는 토종닭 순계 집단을 이용한 3원 교잡을 통해 실용계집단을 생산하는 과정에서 기인한 것으로 추정된다. 집단 및 품종간의 유전적 유연관계를 분석한 결과 토종닭 순계집단 (R, Y)과 실용계집단(C)은 서로간에 가까운 유전적 거리를 유지하고 있는 것으로 확인되었다. 각 MS marker별 품종간의 이형접합률을 이용하여 각 개체들의 집단 내에서 품종을 식별 할 수 있는 확률인 누적품종식별력(CPD) 값을 계산한 결과 13종의 MS marker를 이용하여 개체의 품종을 구분할 수 있는 확률이 99.461%로 나타났다.

• 한국식품영양과학회지
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• 제46권8호
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• pp.952-956
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• 2017

본 연구는 HPLC를 이용하여 율무근 추출물을 기능성 화장품 원료로 개발하기 위하여 지표성분인 coixol의 분석법 설정과 분석법에 대한 검증을 실시하고자 하였다. 표준액과 율무근 추출물은 다른 물질의 간섭 없이 분리되었으며, 표준액과 추출물의 피크 유지시간이 일치한 spectrum을 나타내었다. 또한, blank에서 표준액과 겹치는 피크가 없는 것으로 특이성을 확인하였다. 검량선의 상관계수($R^2$)는 0.9995로 모두 양호한 직선성을 보였으며, 직선상의 검출한계는 0.07~0.08 mg/mL였고 정량한계는 0.23~0.25 mg/mL로 나타났다. 율무근 추출물 2.25, 4.5 및 6.75 mg/mL 세 농도의 회수율은 98.36~100.30%였으며 RSD는 0.26~0.29%로 나타나 RSD 2.0% 이하로서 정확성이 있음을 알 수 있었다. 정밀성은 0.05~0.29%의 정밀도(RSD)를, 실험실 내 정밀성에서는 0.03~0.29%의 정밀도(RSD)를 나타내었고, intra-day에서의 정밀도(RSD)는 0.37~1.64%, inter-day에서는 0.36~0.81%의 정밀도를 나타내어 율무근 추출물의 지표성분 coixol의 분석법은 적합한 시험법임이 검증되었다. 본 분석법은 율무근 추출물의 기능성화장품 원료 개발을 위한 기초자료로 활용될 것으로 생각된다.

• 한국임상수의학회지
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• 제27권6호
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• pp.627-630
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• 2010

본 연구는 중환자의 개에서 전신성염증반응증후군 및 패혈증에 의하여 유발되는 백혈구 활성화 지표를 분석하기 위하여 실행되었다. 분석된 지표로는 백혈구 수치, 혈소판 수치, 혈액 도말 표본의 백혈구 변화상 및 백혈구 표면의 CD11b발현 정도 등을 검사하였다. 그 결과, 패혈증군에서 백혈구의 CD11b 발현 값 (유세포 분석)이 현저하게 높게 관찰되었으며(P<0.05), 퇴행성 변화 정도도 같은 군에서 현저하게 높게 관찰 되었다(P<0.05). 또한 비 생존군의 CD11b 발현 값도 생존군에서의 값보다 현저하게 높았다. 이러한 연구 결과로 볼 때, 백혈구의 CD11b 발현은 중환자의 개에서 그 예후를 평가하는데 유용하다고 여겨진다.

• 한국염색가공학회지
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• 제30권1호
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• pp.29-37
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• 2018

Cotton has no adsorption ability for the cationic dye and heavy metal but, if anionized cotton can be made, it will be possible. In this study, to enable the anionisation of cotton fabric, it was modified using sodium vinylsolfonate(SV) as the anionisation reagent, employing a pad-dry-cure(PDC) technique. The effects of curing time, treatment concentrations of urea, sodium hydroxide and SV on the weight increase were experimented and then, the physical characterizations of sulfoethyl cotton(SEC) depending on the finishing conditions were estimated, thus the application possibility of SV as anionisation reagent was investigated. It was not much changed by anionisation except wrinkle recovery. And the structure of SEC was elucidated by Raman and NMR spectoscopy. The feasibility of using Raman and NMR spectroscopy with the band at $1,043cm^{-1}$, and 50.5ppm, respectively as marker band to determine sulfoethyl group of SEC was reported. The total degree of SV substitution(DSV) was determined via elemental analysis. SEC with diverse total DSV up to 0.066 was obtained. In the thermal decomposition(pyrolysis) by DSC, it can be found that the pyrolysis temperature was about $30^{\circ}C$ lower than that of non-treated cotton fabric.

• Journal of Microbiology and Biotechnology
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• 제6권6호
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• pp.386-390
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• 1996

The ${\beta}$-galactosidase gene from Lactococcus lactis subsp. lactis ATCC 7962 was cloned and its enzymatic properties were characterized, with a view to assessing its potential use as a selection marker in the food-grade cloning vector. Chromosomal DNA from L. lactis subsp. lactis 7962 was cleaved with PstI and ligated into pBR322 for transformation into Escherichia coli TGl. Transformants showing ${\beta}$-galactosidase activity possessed the pBR322 plasmid containing a 10 kilobase (kb) PstI fragment and this plasmid was named pCKL11. The cloned ${\beta}$-galactosidase gene came from the chromosomal DNA of L. lactis subsp. lactis 7962 was confirmed by Southern hybridization. A restriction map of pCKL11 was constructed from the cleavage of both pCKL11 and the purified 10kb insert fraqment. The. optimum pH of the ${\beta}$-galactosidase determined with the E. coli harboring the pCKL11 was 7.0. The optimum temperature was $50^{\circ}C$, while the pI of the enzyme was 7.4. These values were the same as those of the enzyme from the parent strain.

• Journal of Plant Biotechnology
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• 제35권2호
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• pp.121-126
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• 2008

양배추와 무로부터 원형질체를 분리하였고 PEG 처리를 통하여 융합을 하였다. 고농도의 원형질체 혼합물로부터 많은 미소괴를 관찰할 수 있었다. 미소괴는 정상적인 캘러스로 자랐고 이들 캘러스로부터 총 218개의 신초가 재분화 되었다. 재분화된 개체는 순화 과정을 거처 온실에서 재배하였다. 순화된 208개체를 대상으로 마커 검정을 통하여 융합 여부를 확인한 결과, 모두 무의 NWB-CMS에 특이적인 PCR 산물을 확인할 수 없었다. 그러나 ISSR분석을 통하여 208개체 중 3개체에서 양배추와 무의 세포가 융합됨을 확인하였다. 이를 통하여 원형질체의 융합이 성공적으로 일어났음을 증명할 수 있었다. 세포융합이 일어난 3개체는 모두 양배추와 무의 형질을 보이는 중간적인 형태를 가지고 있었다. 이들은 춘화처리를 거쳐 모두 개화 되었으나 꽃의 색깔이 무의 형질과 같은 흰색이었고 이중 한 개체에서만 역교배를 통하여 3개의 종자를 얻었다.

• 생물정신의학
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• 제22권4호
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• pp.205-215
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• 2015

Objectives Sex hormones exposure during the prenatal period has an effect on cerebral lateralization. Male brains are thought to be more lateralized than female brains. Bipolar disorder was known to show abnormalities in cerebral laterality whose characteristics could be estimated by electroencephalography (EEG) coherences. We studied sex-related differences of EEG coherences between healthy controls and patients with bipolar disorder to examine the sex effects in the genesis of bipolar disorder. Methods Participants were 25 patients with bipolar disorder (11 male, 14 female) and 46 healthy controls (23 male, 23 female). EEG was recorded in the eyes closed resting state. To examine dominant EEG coherence associated with sex differences in both groups within five frequency bands (delta, theta, alpha, beta, and gamma) across several brain regions, statistical analyses were performed using analysis of covariance. Results Though statistically meaningful results were not found, some remarkable findings were noted. Healthy control females showed more increased interhemispheric coherences than control males in gamma frequency band. There were no differences in the intrahemispheric coherences between the healthy control males and females. In patients with bipolar disorder, female dominant pattern in interhemispheric coherences was attenuated compared with healthy control. Conclusions Sex differences of EEG coherences, which could be a marker for cerebral laterality, were attenuated in patients with bipolar disorder compared with healthy controls. These results imply that abnormal sex hormone exposure during early development might play some role in the pathogenesis of bipolar disorder.

• Journal of Radiation Protection and Research
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• 제41권3호
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• pp.206-210
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• 2016

Background: Ionizing radiation causes cellular damage and death through the direct damage and/or indirectly the production of ROS, which induces oxidative stress. This study was designed to evaluate the in vivo radioprotective effects of a bio-active material coated fabric (BMCF) against ${\gamma}$-irradiation-induced cellular damage in Sprague-Dawley (SD) rats. Materials and Methods: Healthy male SD rats wore bio-active material coated (concentrations in 10% and 30%) fabric for 7 days after 3 Gy of ${\gamma}$-irradiation. Radioprotective effects were evaluated by performing various biochemical assays including spleen and thymus index, WBC count, hepatic damage marker enzymes [aspartate transaminase (AST) and alanine transaminase (ALT)] in plasma, liver antioxidant enzymes, and mitochondrial activity in muscle. Results and Discussions: Exposure to ${\gamma}$-irradiation resulted in hepatocellular and immune systemic damage. Gamma-irradiation induced decreases in antioxidant enzymes. However, wearing the BMCF-30% decreased significantly AST and ALT activities in plasma. Furthermore, wearing the BMCF-30% increased SOD (superoxide dismutase) and mitochondrial activity. Conclusion: These results suggest that wearing BMCF offers effective radioprotection against ${\gamma}$-irradiation-induced cellular damage in SD rats.

• 한국가금학회지
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• 제32권1호
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• pp.23-27
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• 2005

가금 추백리와 밀접한 관계가 있는 단백질을 동정하기 위하여 인위적으로 추백리를 유도한 개체와 정상인 개체의 간에서 단백질을 추출하였으며, 2차원 전기영동(2DE)과 mass spectrometry(MS)를 이용하여 차등 발현되는 단백질을 조사하였다. 총 300여개의 단백질 spot들이 관찰되었으며, 이중 발현양에 현저한 차이를 보이는 spot을 MALDI-TOF MS와 protein database search를 통해 분석한 결과, 가금 APOAI (Apolipoprotein A1)으로 확인되었다. 추백리가 감염된 닭의 간에서 APOA1 단백질의 증가는 손상된 혈관의 재생과 밀접한 관계가 있으며 추백리의 감염 여부를 나타내 주는 Bio-marker로서 활용될 수 있을 것으로 사료된다.

• Physical Therapy Rehabilitation Science
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• 제8권3호
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• pp.158-161
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• 2019

Objective: Attentional focus is one of the critical factors that has consistently been demonstrated to enhance motor performance and motor skill. Focusing attention on the inside of the body while engaging in a particular exercise is called internal focus (IF) and focus on the external environment is called external focus (EF). The purpose of this study was to identify effects of IF and EF of attention on postural balance in healthy school-aged children. Design: Cross-sectional study. Methods: Twenty-four healthy school-aged children participated in this study. School-aged children was defined as children ages 8-12 years old. They performed the one-legged standing with EF (focusing on the marker at the level of participants' chest and 150 cm away), IF (focusing the supporting feet), and control (no instruction) respectively. The order of the focus condition was randomly selected. The center of pressure (COP) range, distance, and velocity was measured to compare the effects of applying different attentional focuses in the three conditions. Results: The results of our study show that differences in COP range, distance, and velocity among groups were not significant between the different attentional focuses, although all variables of EF were smaller than IF. It is postulated that the reason for this may be that school school-aged children between 8-12 years old go through a transitional phase from IF to EF in effective motor learning. Conclusions: These findings reveal that the type of attentional focus did not have any effect on postural balance in healthy school-aged children.