Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Identification of Upregulated APOA1 Protein of Chicken Liver in Pullorum Disease

추백리가 감염된 닭의 간에서 발현이 증가하는 APOA1 단백질의 확인

  • Jung K. C. (Division of Animal Science and Resources, Chungnam National University) ;
  • Lee Y. J. (Division of Animal Science and Resources, Chungnam National University) ;
  • Yu S. L. (Division of Animal Science and Resources, Chungnam National University) ;
  • Lee J. H. (Division of Animal Science and Resources, Chungnam National University) ;
  • Jang B. K. (National Livestock Research Institute, R.D.A.) ;
  • Koo Y. B. (School of Biotechnology and Biomedical Science, Inje University) ;
  • So H. K. (The Graduate School ol Bio d: Information Technology, Hankyong National University) ;
  • Choi K. D. (The Graduate School ol Bio d: Information Technology, Hankyong National University)
  • 정기철 (충남대학교 동물자원학부) ;
  • 이유주 (충남대학교 동물자원학부) ;
  • 유성란 (충남대학교 동물자원학부) ;
  • 이준헌 (충남대학교 동물자원학부) ;
  • 장병귀 (농촌진흥청 축산연구소) ;
  • 구용범 (인제대학교 생명공학부) ;
  • 소현경 (한경대학교 생물정보통신대학원) ;
  • 최강덕 (한경대학교 생물정보통신대학원)
  • Published : 2005.03.01
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Abstract

The aim of this study was to investigate differentially expressed proteins between normal chicken liver and chicken liver inffeted by Salmonella pullorum. 2-dimensional electrophoresis (2DE) and mass spectrometry (MS) were used to identify the proteins. More than 300 protein spots were detected on silver stained 2DE gels using pH 3$\~$10 gradients. The most outstanding protein spot was further analyzed by MALDI-TOF MS and protein database using the Mascot search engine. The protein was finally identified as APOAI (Apolipoprotein AI). Based on the known function of the APOAI, this gene acts protective action against the accumulation of platelet thrombin at the site of vascular damage for the pullorum disease. Therefore APOAI protein, identified in this study, can be a valuable biomarker in relation to the pullorum disease in chicken.

가금 추백리와 밀접한 관계가 있는 단백질을 동정하기 위하여 인위적으로 추백리를 유도한 개체와 정상인 개체의 간에서 단백질을 추출하였으며, 2차원 전기영동(2DE)과 mass spectrometry(MS)를 이용하여 차등 발현되는 단백질을 조사하였다. 총 300여개의 단백질 spot들이 관찰되었으며, 이중 발현양에 현저한 차이를 보이는 spot을 MALDI-TOF MS와 protein database search를 통해 분석한 결과, 가금 APOAI (Apolipoprotein A1)으로 확인되었다. 추백리가 감염된 닭의 간에서 APOA1 단백질의 증가는 손상된 혈관의 재생과 밀접한 관계가 있으며 추백리의 감염 여부를 나타내 주는 Bio-marker로서 활용될 수 있을 것으로 사료된다.

Keywords

References

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