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Identification of Upregulated APOA1 Protein of Chicken Liver in Pullorum Disease  

Jung K. C. (Division of Animal Science and Resources, Chungnam National University)
Lee Y. J. (Division of Animal Science and Resources, Chungnam National University)
Yu S. L. (Division of Animal Science and Resources, Chungnam National University)
Lee J. H. (Division of Animal Science and Resources, Chungnam National University)
Jang B. K. (National Livestock Research Institute, R.D.A.)
Koo Y. B. (School of Biotechnology and Biomedical Science, Inje University)
So H. K. (The Graduate School ol Bio d: Information Technology, Hankyong National University)
Choi K. D. (The Graduate School ol Bio d: Information Technology, Hankyong National University)
Publication Information
Korean Journal of Poultry Science / v.32, no.1, 2005 , pp. 23-27 More about this Journal
Abstract
The aim of this study was to investigate differentially expressed proteins between normal chicken liver and chicken liver inffeted by Salmonella pullorum. 2-dimensional electrophoresis (2DE) and mass spectrometry (MS) were used to identify the proteins. More than 300 protein spots were detected on silver stained 2DE gels using pH 3$\~$10 gradients. The most outstanding protein spot was further analyzed by MALDI-TOF MS and protein database using the Mascot search engine. The protein was finally identified as APOAI (Apolipoprotein AI). Based on the known function of the APOAI, this gene acts protective action against the accumulation of platelet thrombin at the site of vascular damage for the pullorum disease. Therefore APOAI protein, identified in this study, can be a valuable biomarker in relation to the pullorum disease in chicken.
Keywords
chicken; liver; APOAl; MALDI-TOF; 2DE; pullorum disease;
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