• Journal of the Korean Society for Precision Engineering
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• v.32 no.6
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• pp.577-585
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• 2015

We fabricated multi-scale such as macro-, micro-, and multi-scale wrinkles by using repetitive volume dividing (RVD) method and thermal curing process. Also wrinkle surface was modified with coating of a self-assembled monolayer (SAM). We measured the contact angle of each wrinkled surface, and observed the behavior of droplets on sloping surface. Through experimental study, we found out that the contact angle was much higher in case of multi-scale and SAM coated wrinkles. And micro-scale wrinkle showed a high contact angle comparing with that of macro-scale wrinkle. Dynamic behaviors of a water droplet like sliding velocity on diverse wrinkled surfaces were dependent on their static contact angles. These results showed that hydro-dynamic characteristics were changed depending on the wrinkle structure and the material forming the wrinkle. These dynamic characteristics can be utilized in bio-chip, microfluidics, and many others in order to control easily chemical reactivity.

• Design & Manufacturing
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• v.2 no.1
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• pp.45-50
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• 2008

Micro channel is to fabricate desired pattern on the polymer substrate by pressing the patterned mold against the substrate which is heated above the glass transition temperature, and it is a high throughput fabrication method for bio chip, optical microstructure, etc. due to the simultaneous large area patterning. However, the bad pattern fidelity in large area patterning is one of the obstacles to applying the hot embossing technology for mass production. In the present study, stamper of cross channel with width $100{\mu}m$ and height $50{\mu}m$ was manufactured using UV-LiGA process. Micro channel was manufactured using stamper manufactured in this study. Also replicability appliance was evaluated for micro channel and factors affected replicability were investigated using Taguchi method.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.32 no.10
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• pp.844-849
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• 2008

We present a novel cell deformability monitoring chip based on the digitally measured cell lysis rate which is dependent on the areal strain of the cell membrane. This method offers simple cell deformability monitoring by automated high-throughput testing system. We suggest the filter design considering the areal strain imposed on the cell membrane passing through the filter array having gradually increased orifice length. In the experiment using erythrocytes, we characterized the cell deformability in terms of average fracture areal strain which was $0.24{\pm}0.014\;and\;0.21{\pm}0.002$ for normal and chemically treated erythrocytes, respectively. We also verified that the areal strain of 0.15 effectively discriminates the deformability difference of normal and chemically treated erythrocytes, which can be applied to the clinical situation. We compared the lysis rates and their difference for the samples from different donors and found that the present chips can be commonly used without any calibration process. The experimental results demonstrate the simple structure and high performance of the present cell deformability monitoring chips, applicable to simple and cost-effective cell aging process monitoring.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.25 no.12
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• pp.1844-1852
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• 2001

Recent developments in microfluidic devices based on microelectromechanical systems (MEMS) technique find many practical applications, which include electronic chip cooling devices, power MEMS devices, micro sensors, and bio-medical devices among others. For the design of such micro devices, flows characteristics inside a microchannel have to be clarified which exhibit somewhat different characteristics compared to conventional flows in a macrochannel. In the present study microchannels of various hydraulic diameters are fabricated on a silicon wafer to study the pressure drop characteristics. The effect of abrupt contraction and expansion is also studied. It is found from the results that the friction factor in a straight microchannel is about 15% higher than that in a conventional macrochannel, and the loss coefficients in abrupt expansion and contraction are about 10% higher than that obtained through conventional flow analysis.

• 한국가시화정보학회:학술대회논문집
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• 2004.12a
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• pp.65-74
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• 2004

Measurement of concentration distributions of suspended particles in a micro-channel is out of the most crucial necessities in the area of Lab-on-a-chip to be used for various bio-chemical applications. One most feasible way to measure the concentration field in the micro-channel is using micro-LIF(Laser Induced Fluorescence) method. However, an accurate concentration field at a given cross plane in a micro-channel has not been successfully achieved so far due to various limitations in the light illumination and fluorescence signal detection. The present study demonstrates a novel method to provide an ultra thin laser sheet beam having five(5) microns thickness by use of a micro focus laser line generator. The laser sheet beam illuminates an exact plane of concentration measurement field to increase the signal to noise ratio and considerably reduce the depth uncertainty. Nile Blue A was used as fluorescent dye for the present LIF measurement. The enhancement of the fluorescent intensity signals was performed by a solvent mixture of water $(95\%)$ and ethanol (EtOH)/methanol (MeOH) $(5\%)$ mixture. To reduce the rms errors resulted from the CCD electronic noise and other sources, an expansion of grid size was attempted from $1\times1\;to\;3\times3\;or\;5\times5$ pixel data windows and the pertinent signal-to-noise level has been noticeably increased accordingly.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.110-111
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• 2013

FcBP consisting of 13 amino acids specifically binds to Immunoglobulin G Fc domain. Initially, we utilized this peptide for preparation of antibody chip as a PEG composite for enhanced solubility. After then, the peptide conjugate was immobilized on agarose resin, resulting in highly efficient affinity column for antibody purification. The efficiency was comparable to commercial Protein A column. Recently, this peptide was conjugated with cell penetrating peptide (CPP) on a backbone of GFP, affording antibody transducer, which carries antibody into live cells by simple mixing of antibody and the transducer in cell culture media. Antibody transduction into cells was monitored by live cell imaging. More recently, the FcBP was fused to ferritin cage, which consists of 24 ferritin protein molecules. The FcBP-ferritin cage showed greatly increased binding affinity to human IgG. Its binding was analyzed by QCM and SPR analysis. Finally, it was selectively delivered by Herceptin to SKBR3, a breast cancer cell, over MCF10A, non-tumorigenic cells (Fig. 1). Fig. 1. Fluorescent microscopic images of SKBR3 breast cancer cells (A~C) and MCF10A breast cells (D~F) treated with Cy3-trastuzumab/fFcBP-Pf_Fn complexes. Trastuzumab and FcBP-Pf_Fn, which were labeled with Cy3 (Cy3-trastuzumab) and fluorescein (fFcBP-Pf_Fn), respectively, selectively targeted SKBR3 over MCF10A.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.11 no.6
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• pp.22-27
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• 2012

Drilling process is usually the most efficient and economical method of making a hole in a solid body. However, there have been no analytical method to assess drilling process based on the shear and frictional characteristics. In this paper, procedures for analyzing shear and frictional processes of drilling have been established by adopting an equivalent turning system to drilling. A series of drilling experiments were carried out with varying feed, velocity and drill shape factors. Using the results of the experiments, the cutting characteristics including shear in the primary shear zone and friction in the chip-tool contact region of drilling process have been analyzed. The specific cutting energy tends to decrease exponentially with increase of feed rate. In drilling process 35-40% of the total energy is consumed in the friction process. This is greater than that of turning process in cutting of the same work material.

• Journal of Sensor Science and Technology
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• v.19 no.6
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• pp.403-420
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• 2010

Nano and micro structure-based biosensors are promising tool for label-free detection of biomolecular interactions with great accuracy. This review gives a brief survey on nano and micro platforms to sense a variety of analytes such as DNA, proteins and viruses. Among incredible nano and micro structure for bio-analytical applications, the scope of this paper will be limited to micro and nano resonators and nanowire field-effect transistors. Nanomechanical motion of the resonators transducers biological information to readable signals. They are commonly combined with an optical, capacitive or piezo-resistive detection systems. Binding of target molecule to the modified surface of nanowire modulates the current of the nanowire through electrical field-effect. Both detection methods have advantages of label-free, real-time and high sensitive detection. These structures can be extended to fabricate array-type sensors for multiplexed detection and high-throughput analysis. The biosensors based on these structures will be applied to lab-on-a-chip platforms and point-of-care diagnostics. Basic concepts including detection mechanisms and trends in their fields will be covered in this review.

• Journal of the Korean Society for Precision Engineering
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• v.23 no.8 s.185
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• pp.178-184
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• 2006

Hot embossing is to fabricate desired pattern on the polymer substrate by pressing the patterned mold against the substrate which is heated above the glass transition temperature, and it is a high throughput fabrication method for bio chip, optical microstructure, etc. due to the simultaneous large area patterning. However, the bad pattern fidelity in large area patterning is one of the obstacles to applying the hot embossing technology for mass production. In the present study, PDMS pad was used as a cushion on the backside of the micro-patterned 4 inch Si mold to improve the pattern fidelity over the 4 inch PMMA sheet by increasing the conformal contact between the Si mold and the PMMA sheet. The pattern replicability improvement over 4 inch wafer scale was evaluated by comparing the replicated pattern height and depth for PDMS-cushioned Si mold against the rigid Si mold without PDMS cushion.

• Journal of the Korean Society of Industry Convergence
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• v.22 no.3
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• pp.373-379
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• 2019

Nowadays, the IoT portable electronic devices have become more useful and diverse, so they require various supply voltage levels to operate. This paper presents a DC-DC buck converter with pulse width modulation (PWM) for portable electronic devices. The proposed step-down DC-DC converter consists of passive elements such as capacitors, inductors, and resistors and an integrated chip (IC) for signal control to reduce power consumption and improves ripple voltage with the resolution. The proposed DC-DC converter is simulated and analyzed in PSPICE circuit design platform, and implemented on the prototype PCB board with a Texas Instruments LM5165 IC. The proposed buck converter is showed 92.6% of peak efficiency including a load current range of 4-10 mA, 3.29 mV of the voltage ripple at 5 V output voltage for the supply voltage 12 V. Measured and Simulated power efficiency are made good agreement with each other.