• 한국자기공명학회논문지
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• 제24권1호
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• pp.1-8
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• 2020

An extracellular matrix protein, transforming growth factor-beta-induced protein (TGFBIp/βig-h3), which is induced by transforming growth factor-β in the human cornea, skin, and matrix of many connective tissues, is associated with the adhesion, migration, proliferation, and differentiation of various cells. TGFBIp contains four homologous repeat domains, known as FAS1 domains, where certain mutations have been considered to cause corneal dystrophies. In this study, backbone NMR assignments of FAS1-3/FAS1-4 tandem domain were obtained and compared with those previously known for the isolated FAS1-4 domain. The results corroborate in solution the inter-domain interaction between FAS1-3 and FAS1-4 in TGFBIp.

• 대한의생명과학회지
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• 제25권1호
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• pp.83-91
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• 2019

This study was undertaken to investigate the therapeutic effect of Angelica acutiloba on allergic dermatitis, which Angelica acutiloba is traditionally known to have antiinflammatory effects. Sprague-Dawley (SD) rats were divided into five groups: normal control group, experimental allergic dermatitis group (vehicle group), low dose of Angelica acutiloba extract (low-dose group), high dose of Angelica acutiloba extract (high-dose group), and antihistamine treated group with experimental dermatitis (antihistamine group). Total leukocyte, neutrophil, lymphocyte, monocyte and eosinophil counts were significantly higher in the vehicle group than in the control group, but these variables were significantly lower in the low- and high-dose groups than in the vehicle group. The platelet/lymphocyte ratio in the red blood cell index was significantly lower in the low- and high-dose groups than in the vehicle group. Low and high doses of the Angelica acutiloba extract did not have toxic effects on liver and kidney. Serum NO, iNOS and levels were highest in the vehicle group but significantly lower in the low- and high-dose groups, especially in the high-dose group. The results of this study suggested that the Angelica acutiloba extract had the effect of alleviating or treating the experimental allergic dermatitis, and it was concluded that the high dose was more effective.

• 한국식생활문화학회지
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• 제36권5호
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• pp.430-440
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• 2021

The purpose of this study was to provide basic data for future research and suggest product development and market segmentation strategies by identifying the perceptions and consumption behaviors of vegetarians in each segment of the vegetarian market. According to food consumption value, a total of three market segments were derived, and the markets were named based on their characteristics, including 'environment and animal protection', 'multiple consideration' and 'low interest'. As a result of analyzing the perception of vegetarian-based food, "environment and animal protection" and "multiple consideration" presented positive perceptions of a vegetarian food product. Conversely, the group stating low interest expressed negative perceptions of the vegetarian food product. An analysis of the requirements for development for vegetarian-based foods products (eg. aquafaba), indicated that the development requirements for all products, except cultured and processed meats, were high.?Considerable demand was observed for vegetarian menus and vegetarian restaurants. As a result of the analysis on the necessity of the elements of the vegetarian restaurant menu, the subjects that stated "multiple consideration" had significantly higher awareness of issues related to vegetarian foods than others.

• 한국가축번식학회지
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• 제17권3호
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• pp.263-267
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• 1993

Transgenic animals have become an important tool in the basic and applied sectors of genetic and biomedical sciences. In particular transgenes provide clear-cut markers in the spatial and temporal analysis of developing embryos for the understanding of developmental mechanisms. For the long-term use of plasmid vector in a particular purpose it would be necessary to develop one's own vector system which can be properly expressed in eukaryotic system. Plasmids were constructed from ori region of pUC19 and early region of SV40 through various steps. LacZ gene coding for $\beta$-galactosides was fused to early gene of SV40 in translational in-frame. Poly(A) tailing site of SV40 was inserted at the 3' lacZ so that initiation, elongation and terminatin be controlled by SV40 transcription (pSS4). Biological function of the constructed pSS4 was demonstrated via microinjection of the plasmid into fertilized loach eggs and subsequent detection of $\beta$-galactosidase in developing embryos. The result indicate that the newly constructed pSS4 is functional in a eukaryotic system in vivo. Thus pSS4 may be used as an efficient tool for the study of embryogenesis and a basic carrier for various genes for animal transgenesis.

• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.1024-1030
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• 2007

We investigated the human apolipoprotein E2 (apoE2) transgenic mouse as an animal model system for age-related macular degeneration (AMD). Transgenic mice expressing human apoE2 and C57BL/6J mice were fed normal chow or a high-fat diet for 4 weeks. Eyes were collected from the mice and lipid deposits in retinal pigment epithelium (RPE) were assessed using electron microscopy. The expressions of apoE, vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and pigment-epithelium derived factor (PEDF), which are molecular markers for angiogenesis, were assessed with immunohistochemistry. Eyes from apoE2 mice, regardless of diet, contained lipid accumulation in RPE under electron microscopy, whereas control C57BL/6J eyes did not. Lipid accumulation was found predominantly in the RPE and the Bruch's membrane and increased in the eyes of apoE2 mice after one month of a high-fat diet ($8{\pm}2\;per\;50{\mu}m^2$ for normal chow and $11{\pm}2\;per\;50\;{\mu}m^2,\;p<0.05)$. ApoE expression was similar in the apoE2 and control mice; however, VEGF and bFGF were overexpressed in the retinal pigment epithelium of apoE2 eyes compared with control eyes, and PEDF expression was slightly decreased. These expression patterns of VEGF, bFGF, and PEDF suggest angiogenesis is progressing in apoE2 eyes. In conclusion, the eyes of apoE2 mice develop typical lipid accumulations, a common characteristic of AMD, making them a suitable animal model for AMD. The expression profile of VEGF and bFGF on the retinal pigment epithelium suggests that apoE2 may induce neovascularization by altering angiogenic cytokines.

• Journal of Plant Biotechnology
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• 제40권4호
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• pp.224-230
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• 2013

본 연구 결과 질산은과 구연산 및 비타민 C의 처리는 레드플레임의 갈변화 방지에는 효과적이나, 레드플레임 인편의 재분화 및 생장에 있어 비타민 C와 질산은의 처리는 효과적 이지 못하였다. 반면 구연산의 경우 레드플레임의 재분화 및 생장에 도움을 주는 것으로 나타났으나 $150mg{\cdot}l^{-1}$이상의 고농도에서는 생육이 저해됨을 보여 $100mg{\cdot}l^{-1}$ 처리가 나리의 재분화 및 생장에 가장 효율적임을 확인하였다. 또한 절편체의 갈변화를 감소시키는 부분에서는 비타민 C $150mg{\cdot}l^{-1}$ 처리가 1.5%의 가장 낮은 갈변율을 나타내어 갈변화 현상 감소에 가장 적합한 처리구로 판단되었다. 본 실험결과는 향후 나리기내 식물체생산에 문제점으로 발생하는 갈변화 현상을 감소시키고 인편 재분화 및 생장을 촉진시켜 우량 나리 기내 묘의 대량증식체계 확립을 통한 우량종구묘 생산체계 확립에 기여하여 농가 소득 향상에 도움이 될 것으로 판단된다.

• 생명과학회지
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• 제31권9호
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• pp.862-872
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• 2021

최근 생물의학 분야에서의 광범위한 응용 가능성에 의하여 식물이나 미생물을 이용한 은(Ag), 금(Au), 백금(Pt), 세륨(Ce), 아연(Zn), 구리(Cu) 등의 금속 나노물질 합성에 관한 연구가 주목받고 있다. 식물은 플라보노이드, 알칼로이드, 사포닌, 스테로이드 탄닌과 각종 영양 성분과 같은 생리 활성 물질을 풍부하게 가지고 있으며, 유사하게 미생물들도 단백질과 같은 생리활성 대사산물이나 색소, 항생제 및 산과 같은 가치가 있는 화학물질을 분비한다. 최근 보고된 바에 의하면, 나노입자의 생체 합성은 무해한 방법일 뿐만 아니라 항균, 항진균, 세포 증식 억제 및 항플라스모디아 활성과 같은 생물의학 분야로서의 적용에 주요한 후보로 여겨진다. 나노입자의 이러한 생리 활성은 농도에 의존적이며, 나노입자의 모양과 크기에도 따라 달라질 수 있다. 미생물과 식물은 나노입자의 친환경적합성에 사용되는 대사산물이나 화학물질 등의 훌륭한 공급원으로서 생물 의학 분야에서 유용하게 사용된다. 미생물 또는 식물 원료를 사용하여 합성된 나노입자는 화학적인 방법으로 합성된 나노입자보다 더 낮은 독성을 나타낸다. 본 리뷰논문에서는 미생물이나 식물과 같은 생물학적 재료를 이용한 나노입자의 합성과, 합성에 사용되는 다양한 기술의 특성 및 나노입자의 항균 분야에서의 적용에 대하여 중점적으로 서술하였다.

• Natural Product Sciences
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• 제9권4호
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• pp.232-234
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• 2003

A new glycerol ether has been isolated from a marine sponge Stelletta sp. By bioactivity-guided fractionation. The structure was established on the basis of NMR and MS analyses. The compound was evaluated for cytotoxicity against a small panel of five human tumor cell lines and exhibited significant cytotoxicity.

• Natural Product Sciences
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• 제9권1호
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• pp.18-21
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• 2003

Guided by the brine shrimp lethality assay, a new (4) and three known cyclitol derivatives (1-3) were isolated from the marine sponge Stelletta sp. Norsarcotride A (4) showed significant cytotoxicity against a small panel of five human tumor cell lines.

• Journal of Ginseng Research
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• 제47권2호
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• pp.349-351
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• 2023