• Journal of Bio-Environment Control
• /
• v.30 no.4
• /
• pp.287-294
• /
• 2021

In order to expand facility agriculture and reduce greenhouse construction costs in reclaimed land, a greenhouse foundation method that satisfies economic feasibility and structural safety at the same time is required. As an alternative, the allowable bearing capacity and settlement were reviewed when the DCM(Deep cement mixing) method was applied among the soft ground reinforcement methods. To examine the applicability of the greenhouse foundation, the allowable bearing capacity and settlement were calculated by applying the theory of Terzaghi, Meyerhof, Hansen, and Schmertmann. In case of the diameter of 800mm and the width and length of the foundation of 4m, the allowable bearing capacity was 179kN/m² and the settlement was 7.25mm, which satisfies the required bearing capacity and settlement standards. The calculation results were verified through FEM(Finite element method) analysis using the Mohr-Coulomb material model. The allowable bearing capacity was 169kN/m² and the settlement was 2.52mm. The bearing capacity showed an error of 5.6% compared to calculated value, and the settlement showed and error of 65.4%. Through theoretical calculations and FEM analysis, it was confirmed that the allowable bearing capacity and settlement satisfies the design criteria as a greenhouse foundation when the width and length of the foundation were 4m. Based on the verified design values, it is expected to be able to present the foundation design criteria for greenhouses through empirical tests such as bearing capacity tests and long-term settlement monitoring.

• Journal of Bio-Environment Control
• /
• v.30 no.4
• /
• pp.419-428
• /
• 2021

In order to develope a mobile-based greenhouse energy calculation program, firstly, the overall thermal transmittance of 10 types of major covers and 16 types of insulation materials were measured. In addition, to estimate the overall thermal transmittance when the cover and insulation materials were installed in double or triple layers, 24 combinations of double installations and 59 combinations of triple installations were measured using the hotbox. Also, the overall thermal transmittance value for a single material and the thermal resistance value were used to calculate the overall thermal transmittance value at the time of multi-layer installation of covering and insulating materials, and the linear regression equation was derived to correct the error with the measured values. As a result of developing the model for estimating thermal transmittance when installing multiple layers of coverings and insulating materials based on the value of overall thermal transmittance of a single-material, the model evaluation index was 0.90 (good when it is 0.5 or more), indicating that the estimated value was very close to the actual value. In addition, as a result of the on-site test, it was evaluated that the estimated heat saving rate was smaller than the actual value with a relative error of 2%. Based on these results, a mobile-based greenhouse energy calculation program was developed that was implemented as an HTML5 standard web-based mobile web application and was designed to work with various mobile device and PC browsers with N-Screen support. It had functions to provides the overall thermal transmittance(heating load coefficient) for each combination of greenhouse coverings and thermal insulation materials and to evaluate the energy consumption during a specific period of the target greenhouse. It was estimated that an energy-saving greenhouse design would be possible with the optimal selection of coverings and insulation materials according to the region and shape of the greenhouse.

• Journal of Bio-Environment Control
• /
• v.31 no.4
• /
• pp.343-355
• /
• 2022

This study investigated the consumption tendency and awareness of paprika in order to expand and promote the consumption of Capsicum annuum L. The research investigated the relationship of preference and loyalty based on emotional response of paprika according to the semantic differential scale. The survey was conducted from January to February 2022 using a random sampling method targeting 155 general people, and a total of 142 questionnaires were analyzed excluding 13 wrong answers. The nine items on the awareness of paprika showed to be consisted of three factors such as 'Food taste', 'Usability', and 'Economics' by factor analysis. Regarding to the awareness of paprika the positive answer that 'I think paprika is good for health' among the nine questions was the highest at 92.3%. In the preference aspect of shape, blocky type had the highest preference for the shape of paprika, followed by mini and conical types in order of preference (p < 0.001). As for color preference, yellow paprika was the most preferred, followed by orange, red, and green, showing statistical significance. The emotional response of paprika by paprika image showed a statistically significant difference in the four colors. The words such as 'bright', 'clean', and 'spirited' appeared as representative emotional vocabulary for paprika. Multiple regression analysis was performed to examine the effect of paprika on the three factors of awareness, preference, and loyalty due to the quality of life. As a result, the higher the paprika preference and quality of life, and the higher the taste and availability factors, the higher the paprika awareness and loyalty. As the variable that has the most influence on the loyalty of the survey respondents, preference was found to have the highest explanatory power at 43%. From these results, it was judged as a very important factor in the survey on the shape and color preference of paprika. Therefore, the recent increase in awareness that paprika is good for health is thought to act as a positive factor in revitalizing the domestic market and increasing consumption of paprika in the future. Also, among the three types of paprika, the yellow blunt type showed the highest preference. Therefore, in order to produce and promote this type of paprika, it is also important to increase the cultivation to suit the purchasing propensity of consumers.

• Journal of Bio-Environment Control
• /
• v.31 no.2
• /
• pp.133-141
• /
• 2022

Bulbophyllum auricomum Lindl. is a rare orchid and has flowers with an attractive fragrance. The present study investigated the tissue culture method for micropropagation. Capsules derived from artificial self-pollination were obtained for the best seed germination in MS basal medium. Plant growth regulators (1.0 mg·L^-1 of BAP and 2.0 mg·L^-1 of NAA) were affected by callus induction from subcultured pseudobulb explants. For the callus subculture, different natural plant extracts were tested in 11 treatment media. Among them, MS medium with 150 mL·L^-1 of coconut water was generally effective in fresh weight (1.75 ± 0.08) and (3.01 ± 0.20) of callus proliferation and PLBs induction at 1 and 2 months, respectively, followed by an MS combination of 30 g·L^-1 of banana and 20 g·L^-1 of potato extract. The results of a comparative study of different MS mediums containing plant growth regulators with a natural extract combination and MS medium supplemented with natural extract only showed that MS medium supplemented with a combination of natural extracts (150 mL·L^-1 of coconut water) and plant growth regulators (2.0 mg·L^-1 of BAP and 1.0 mg·L^-1 of NAA) obtained the highest shoot regeneration (3.37 ± 0.17) and (6.41 ± 0.68) after 1 month and 2 months of culturing, respectively.

• Journal of Bio-Environment Control
• /
• v.32 no.1
• /
• pp.48-56
• /
• 2023

High-pressure sodium (HPS) lamps have been widely used as a useful supplemental light source to emit sufficient photosynthetically active radiation and provide a radiant heat, which contribute the heat requirement in greenhouses. The objective of this study to analyze the thermal characteristics of HPS lamp and thermal behavior in supplemented greenhouse, and evaluate the performance of a horizontal leaf temperature of sweet pepper plants using computational fluid dynamics (CFD) simulation. We simulated horizontal leaf temperature on upper canopy according to three growth stage scenarios, which represented 1.0, 1.6, and 2.2 plant height, respectively. We also measured vertical leaf and air temperature accompanied by heat generation of HPS lamps. There was large leaf to air temperature differential due to non-uniformity in temperature. In our numerical calculation, thermal energy of HPS lamps contributed of 50.1% the total heat requirement on Dec. 2022. The CFD model was validated by comparing measured and simulated data at the same operating condition. Mean absolute error and root mean square error were below 0.5, which means the CFD simulation values were highly accurate. Our result about vertical leaf and air temperature can be used in decision making for efficient thermal energy management and crop growth.

• Korean Journal of Poultry Science
• /
• v.35 no.4
• /
• pp.375-380
• /
• 2009

This study was conducted to investigate the effect of feeding induced molting on the visceral organs and blood component profile in laying hens and designed to test 400 flocks of 60 week old Leghorn laying hens for 34 weeks. A total of four molting treatment methods by including the molted with customary molting by fasting method (c), feeding single diet of corn (T1), feeding single diet of wheat bran (T2) and feeding single diet of alfalfa meal (T3) were tested, and each treatment was repeated for 5 times, and 20 laying hens were randomly assigned in an cage for each repeat. As the result of the experiment, ovary was $2.03{\sim}6%$ and oviduct was $2.51{\sim}3.47%$ in visceral organs for body weight at pre-molting term, but there was no significant difference. At post-molting, no significant difference was found, ovary was $0.25{\sim}0.41%$, uterus of control, T1, T2 and T3 was 1.12%, 0.82%, 0.48% and 0.90%, respectively. T2 was significantly lower than control, T3 (p<0.05) at the 50% of egg production. Ovary was $2.20{\sim}2.60%$ and oviduct was $2.98{\sim}3.45%$. In addition, ovary was $2.65{\sim}3.01%$, oviduct was $3.23{\sim}3.64%$ at the peak egg production, but there was no significant difference by non-feeding and feeding molting treatments. In blood component profile, cholesterol was $179.8{\sim}245.7\;mg/dL$ at pre-molting, but there was no significant difference and at post-molting, concentration of cholestrol in control, T1, T2 and T3 was 353.6, 229.1, 261.8 and 300.6 mg/dL, respectively. T1 was significantly lower than control and T3 (p<0.05). In addition, first laying day was $228.1{\sim}271.8\;mg/dL$, 50% of egg production was $236.5{\sim}284.8\;mg/dL$, there was no significant difference. Concentration of cholestrol in control, T1, T2 and T3 was 324.1, 591.6, 363.0 and 315.6 mg/dL, respectively, at the peak egg production period. T1 was significantly higher than other treatment (p<0.05).

• Korean Journal of Poultry Science
• /
• v.42 no.1
• /
• pp.33-40
• /
• 2015

This study was conducted to investigate the effects of dietary quercetin on growth performance, blood biochemical parameters, immunoglobulin, and blood antioxidant activity in broiler chickens. Three hundred twenty one-day old Ross broilers were divided 8 treatments (C(-), basal diet; C(+), basal diet with antibiotics; vitamin E 20 IU; vitamin E 200 IU; quercetin 20 ppm; quercetin 200 ppm; methoxylated quercetin 20 ppm; methoxylated quercetin 200 ppm) with 4 replicates and 10 birds per replicate. Birds were reared for 35 days and their feed intake and weight gain were measured weekly. At 35d, eight birds of average weight from each replicate were selected for blood collection and analysis. Weight gain of birds in the groups fed quercetin was higher when compare to NC but there was no significant difference. In the serum, creatinine, BUN and AST in quercetin groups significantly decreased compared to those of control (NC and PC) (P<0.05). The contents of IgA and IgM were significantly lower in quercetin groups than those of NC (P<0.05). SOD like activity and MDA content tended to decrease in quercetin groups, however, there was no significant difference among treatments. In conclusion, supplemental quercetin to poultry diet could be positive aspect on performance and blood metabolites. Optimum adding levels was more than 20 ppm.

• Journal of Life Science
• /
• v.28 no.7
• /
• pp.835-840
• /
• 2018

Quantitative determination of the protein expression levels is one of the most important parts in assessment of the safety of foods derived from genetically modified (GM) crops. Overexpression of AtCYP78A7, a gene encoding cytochrome P450 protein, has been reported to improve tolerance to abiotic stress, such as drought and salt stress, in transgenic rice (Oryza sativa L.). In the present study, an enzyme-linked immunosorbent assay (ELISA) kit for diagnosing AtCYP78A7 protein including AtCYP78A7-specific monoclonal antibody was developed. GST-AtCYP78A7 recombinant protein was induced and purified by affinity column. Four monoclonal antibodies (mAb 6A7, mAb 4C2, mAb 11H6, and mAb 7E8) against recombinant protein were also produced and biotinylated with avidin-HRP. After pairing test using GST-AtCYP78A7 protein and lysate of rice samples, mAb 4C2 and mAb 7E8 were selected as a capture antibody and a detecting antibody, respectively, for ELISA kit. Product test using rice samples indicated that percentages of detected protein in total protein were greater than 0.1% in AtCYP78A7-overexpressing transgenic rice (Line 10B-5 and 18A-4), whereas those in negative control non-transgenic rice (Ilpum and Hwayoung) were less than 0.1%. The ELISA kit developed in this study can be useful for the rapid detection and safety assessment of transgenic rice overexpressing AtCYP78A7.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.15 no.3
• /
• pp.263-268
• /
• 2009

The Yellow Sea is extremely important to the economy and to the health and well-being of surrounding countries, Korea and China. Recently, the Yellow Sea is under constantly increasing threat of degradation due to the increase of environmental pollution and over-fishing. The governments of Korea and China have been aware of the importance of the Yellow Sea and reached an Environmental Agreement between Korea and China at the governmental level(November, 1993) According to this environmental agreement the Yellow Sea Environmental Cooperative Research between Korea and China has been undertaken since 1997. The joint cruise had been conducted once a year at 33 stations in the 4 lines of the Yellow Sea where the 9 stations of the D line was newly added in the 7th cruise in 2003. The samples were analyzed by scientists of both countries at the WSFRI, Korea and the OEMNC of the SEPA, China in turn, the annual report has been published every year during 1998-2008. The scientific efforts to fix the cruise time in October and to extend research frequency, as twice a year, should be considered, and this requires the governmental supports such as research funds and other related administrational assistance on both sides. Finally, scientists should also pay a concentrated attention to standardize the analytical methods including quality control and to improve this Yellow Sea research as one of the most representative international projects in the Yellow Sea where sharing additional informations available, if exist of dumping sites and material content, and of the freshwater quality will be of great help to broaden the output of this joint research project.

• Journal of Life Science
• /
• v.15 no.5 s.72
• /
• pp.819-825
• /
• 2005

The Antioxidative accvities of the cell free extracts containing high glutathione by Saccharomyces cerevisiae FF-8 were tested in vitro experimental models : DPPH method for radical scavenging activity, ferric TBA method and ferric thiocyanate method using linoleic acid and tissue microsome for lipid peroxidation inhibitions. The concentration of intercellular glutathione by cultivating S. cerevisiae FF-8 in the YM optimal medium obtained $204\mug/ml$, which was increased by 2.76-fold from $74\mug/ml$ in the YM basal medium. A comparition between the YM basal medium and the YM optimal medium on antioxidative substance produced by S. cerevisiae FF-8 was investigated. In DPPH ($\alpha, \alpha-diphenyl-\beta-picrylhydrazyl$) method, the electron donating activity of the glutathione produced by S. cerevisiae FF-8 cultured in the YM optimal medium was as high as that of BHT ($ 0.05\%w/v $). The antioxidative a.tivity was measured by inhibition against lipid peroxidation of rat tissues' microsomes. The results of anti-oxidant activity of the cell free extracts by S. rerevisiae FF-8 cultured in the YM optimal medium was shown in the following order . $ liver 60.98\% > kidney 56.43\% > heart 52.91\% > brain 52.13\% > testis 45.57\% > spleen 42.95\% $. In antioxidative activities determined by ferric thiocyanate method and TBA methods against lipid peroxidation, the lipid peroxidation in the control mixture increased more rapidly than the typical peroxidation curve of linoleic acid from one day. The antioxidative activity of the cell free extracts by cultivating S. cerevisine FF-8 in the YM optimal medium were higher than that of the YM basal medium. These data indicate that the cell free extracts containing a high intercellular glutathione of S. cerevisiae FF-8 cultured in YM optimal medium showed strong antioxidative capacities by DPPH radical scavenging activity and ferric thiocyanate and TBARS measurements.