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http://dx.doi.org/10.12791/KSBEC.2022.31.2.133

Effects of Different Natural Extracts and Plant Growth Regulators on Plant Regeneration and Callus Induction from Pseudobulbs Explants through in vitro Seed Germination of Endangered Orchid Bulbophyllum auricomum Lindl.  

Aung, Win Theingi (Department of Lifestyle Medicine, College of Environmental and Bioresource Sciences, Jeonbuk National University)
Bang, Keuk Soo (Department of Oriental Medicine Resources, College of Environmental and Bioresource Sciences, Jeonbuk National University)
Yoon, Seo A (Institute of Life Science and Natural Resources, Wonkwang University)
Ko, Baul (Urban Agriculture Division. National Institute of Horticultural & Herbal Science, RDA)
Bae, Jong Hyang (Department of Horticulture Industry, Wonkwang University)
Publication Information
Journal of Bio-Environment Control / v.31, no.2, 2022 , pp. 133-141 More about this Journal
Abstract
Bulbophyllum auricomum Lindl. is a rare orchid and has flowers with an attractive fragrance. The present study investigated the tissue culture method for micropropagation. Capsules derived from artificial self-pollination were obtained for the best seed germination in MS basal medium. Plant growth regulators (1.0 mg·L-1 of BAP and 2.0 mg·L-1 of NAA) were affected by callus induction from subcultured pseudobulb explants. For the callus subculture, different natural plant extracts were tested in 11 treatment media. Among them, MS medium with 150 mL·L-1 of coconut water was generally effective in fresh weight (1.75 ± 0.08) and (3.01 ± 0.20) of callus proliferation and PLBs induction at 1 and 2 months, respectively, followed by an MS combination of 30 g·L-1 of banana and 20 g·L-1 of potato extract. The results of a comparative study of different MS mediums containing plant growth regulators with a natural extract combination and MS medium supplemented with natural extract only showed that MS medium supplemented with a combination of natural extracts (150 mL·L-1 of coconut water) and plant growth regulators (2.0 mg·L-1 of BAP and 1.0 mg·L-1 of NAA) obtained the highest shoot regeneration (3.37 ± 0.17) and (6.41 ± 0.68) after 1 month and 2 months of culturing, respectively.
Keywords
artificial self-pollination; tissue culture; natural extract; plant growth regulator;
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