• Journal of Applied Biological Chemistry
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• 제64권4호
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• pp.433-438
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• 2021

폴리에틸렌은 농업용 필름으로 널리 사용되고 있지만, 이를 친환경적으로 분해하기 위한 기술은 부족한 상황이다. 이에 최근 폴리에틸렌 분해를 위한 친환경 기술 개발에 대한 관심이 높아지고 있다. 폴레에틸렌의 생물학적 분해에는 몇가지 산화 단계가 필요할 것이라고 예상된다. 먼저, 폴리에틸렌 사슬에 2차 알코올이 형성되고, 알코올은 카르보닐기로 산화된다. 이후 과정에서 카르보닐기는 Baeyer-Villiger monooxygenase (BVMO)에 의해 에스터로 전환되고, 이 에스터는 마지막 단계에서 lipase와 esterase에 의해 절단될 것으로 예상된다. 본 연구에서는 폴리에틸렌 분해 과정에 관여하는 중요한 효소 중 하나인 BVMO의 반응 메커니즘, 분류, 효소공학 측면에서 리뷰하였다. 또한 BVMO를 사용한 폴리에틸렌 분해 분야의 향후 연구전망도 간략히 덧붙였다.

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1083-1089
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• 2007

Because of their selectivity and catalytic efficiency, BVMOs are highly valuable biocatalysts for the chemoenzymatic synthesis of a broad range of useful compounds. In this study, we investigated the microbial Baeyer-Villiger oxidation and sulfoxidation of thioanisole and bicyclo[3.2.0]hept-2-en-6-one using whole Escherichia coli cells that recombined with each of the Baeyer-Villiger monooxygenases originated from Pseudomonas aeruginosa PAOl and two from Streptomyces coelicolor A3(2). The three BVMOs were identified in the microbial genome database by a recently described protein sequence motif; e.g., BVMO motif(FXGXXXHXXXW). The reaction products were identified as (R)-/(S)-sulfoxide and 2-oxabicyclo/3-oxabicyclo[3.3.0]oct-6-en-2-one by GC-MS analysis. Consequently, this study demonstrated that the three enzymes can indeed catalyze the Baeyer-Villiger reaction as a biocatalyst, and effective annotation tools can be efficiently exploited as a source of novel BVMOs.

• Journal of Microbiology and Biotechnology
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• 제22권6호
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• pp.832-837
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• 2012

The oxidative potential of the fungus Penicillium brasilianum, a strain isolated as endophytic from a Meliaceae plant (Melia azedarach), was investigated using 1-indanone as substrate to track the production of monooxygenases. The fungus produced the dihydrocoumarin from 1-indanone with the classical Baeyer-Villiger reaction regiochemistry, and (-)-(R)-3-hydroxy-1-indanone with 78% ee. Minor compounds that had resulted from lipase and SAM activities were also detected. The biotransformation procedures were also applied using a collection of Penicillium and Aspergillus fungi obtained from M. azedarach and Murraya paniculata. The results showed that Baeyer-Villiger were mostly active in fungi isolated from M. azedarach. Almost all fungi tested produced 3-hydroxy-1-indanone.