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The Analysis and Application of a Recombinant Monooxygenase Library as a Biocatalyst for the Baeyer- Villiger Reaction  

Park, Ji-Yeoun (Korea Research Institute of Bioscience and Biotechnology)
Kim, Dong-Hyun (Korea Research Institute of Bioscience and Biotechnology)
Kim, Su-Jin (Korea Research Institute of Bioscience and Biotechnology)
Kim, Jin-Hee (Korea Research Institute of Bioscience and Biotechnology)
Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
Lee, Choong-Hwan (Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.7, 2007 , pp. 1083-1089 More about this Journal
Abstract
Because of their selectivity and catalytic efficiency, BVMOs are highly valuable biocatalysts for the chemoenzymatic synthesis of a broad range of useful compounds. In this study, we investigated the microbial Baeyer-Villiger oxidation and sulfoxidation of thioanisole and bicyclo[3.2.0]hept-2-en-6-one using whole Escherichia coli cells that recombined with each of the Baeyer-Villiger monooxygenases originated from Pseudomonas aeruginosa PAOl and two from Streptomyces coelicolor A3(2). The three BVMOs were identified in the microbial genome database by a recently described protein sequence motif; e.g., BVMO motif(FXGXXXHXXXW). The reaction products were identified as (R)-/(S)-sulfoxide and 2-oxabicyclo/3-oxabicyclo[3.3.0]oct-6-en-2-one by GC-MS analysis. Consequently, this study demonstrated that the three enzymes can indeed catalyze the Baeyer-Villiger reaction as a biocatalyst, and effective annotation tools can be efficiently exploited as a source of novel BVMOs.
Keywords
Baeyer-Villiger oxidation; sulfide oxidation; monooxygenase; biocatalysis; GC-MS;
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Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
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